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Strain Name:
C57BL/6J-MtgxR4231Btlr/Mmmh
Stock Number:
041050-MU
Citation ID:
RRID:MMRRC_041050-MU
Other Names:
R4231 (G1), C57BL/6J-MtgxR4231Btlr
Major Collection:

Strain Information

Myo5a
Name: myosin VA
Synonyms: MVa, MyoVA, Myo5, flail, 9630007J19Rik, Dbv
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 17918
HGNC: HGNC:7602
Homologene: 20100
Casp8
Name: caspase 8
Synonyms: Mch5, MACH, FLICE, Caspase-8
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12370
HGNC: HGNC:1509
Homologene: 7657
Arhgef18
Name: Rho/Rac guanine nucleotide exchange factor 18
Synonyms: D030053O22Rik, A430078G23Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 102098
Homologene: 32254
Senp2
Name: SUMO/sentrin specific peptidase 2
Synonyms: 4930538C18Rik, 2310007L05Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 75826
VEGA: 16
Homologene: 11005
Kif20a
Name: kinesin family member 20A
Synonyms: Rabkinesin-6, Rab6kifl
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 19348
VEGA: 18
HGNC: HGNC:9787
Homologene: 38093
Atg14
Name: autophagy related 14
Synonyms: D14Ertd114e, D14Ertd436e, Barkor
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 100504663
VEGA: 14
Homologene: 45423
Ddx1
Name: DEAD box helicase 1
Synonyms: DEAD (Asp-Glu-Ala-Asp) box polypeptide 1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 104721
VEGA: 12
HGNC: HGNC:2734
Homologene: 3627
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 58,114,885 bp
  • T to C, chromosome 1 at 58,844,770 bp
  • T to C, chromosome 1 at 72,658,542 bp
  • T to C, chromosome 1 at 74,147,663 bp
  • A to G, chromosome 1 at 97,884,124 bp
  • A to G, chromosome 1 at 190,808,900 bp
  • C to T, chromosome 1 at 194,644,454 bp
  • A to G, chromosome 2 at 14,981,440 bp
  • T to C, chromosome 2 at 30,413,011 bp
  • A to T, chromosome 2 at 60,668,521 bp
  • C to T, chromosome 2 at 111,528,201 bp
  • A to T, chromosome 2 at 130,044,589 bp
  • C to T, chromosome 2 at 145,860,446 bp
  • A to T, chromosome 2 at 148,442,960 bp
  • C to T, chromosome 3 at 36,920,236 bp
  • T to C, chromosome 3 at 51,542,730 bp
  • T to C, chromosome 3 at 54,633,083 bp
  • T to G, chromosome 3 at 59,257,223 bp
  • A to G, chromosome 3 at 87,740,991 bp
  • C to A, chromosome 4 at 114,244,659 bp
  • A to T, chromosome 4 at 121,622,102 bp
  • C to T, chromosome 4 at 141,465,074 bp
  • T to C, chromosome 4 at 148,617,601 bp
  • G to A, chromosome 5 at 57,719,289 bp
  • A to G, chromosome 5 at 113,560,984 bp
  • A to G, chromosome 5 at 113,771,418 bp
  • A to G, chromosome 5 at 139,108,621 bp
  • T to C, chromosome 5 at 142,971,966 bp
  • A to T, chromosome 5 at 143,093,090 bp
  • A to G, chromosome 6 at 113,690,856 bp
  • T to C, chromosome 7 at 4,752,835 bp
  • T to C, chromosome 7 at 126,174,182 bp
  • T to C, chromosome 7 at 140,652,740 bp
  • T to C, chromosome 8 at 3,450,317 bp
  • T to C, chromosome 8 at 33,572,137 bp
  • T to A, chromosome 8 at 71,685,545 bp
  • T to A, chromosome 9 at 19,441,590 bp
  • T to C, chromosome 9 at 21,756,202 bp
  • T to A, chromosome 9 at 22,249,745 bp
  • A to T, chromosome 9 at 48,398,837 bp
  • A to T, chromosome 9 at 72,619,390 bp
  • A to G, chromosome 9 at 75,189,997 bp
  • A to G, chromosome 9 at 80,345,329 bp
  • G to A, chromosome 9 at 108,805,648 bp
  • G to T, chromosome 9 at 119,631,544 bp
  • G to A, chromosome 10 at 76,319,470 bp
  • A to T, chromosome 10 at 84,814,694 bp
  • A to T, chromosome 10 at 107,686,283 bp
  • A to G, chromosome 10 at 127,193,189 bp
  • G to A, chromosome 11 at 5,243,881 bp
  • A to T, chromosome 11 at 34,403,143 bp
  • C to T, chromosome 11 at 58,219,478 bp
  • C to T, chromosome 12 at 13,223,857 bp
  • A to G, chromosome 12 at 13,393,343 bp
  • A to T, chromosome 12 at 53,141,038 bp
  • T to C, chromosome 12 at 113,135,827 bp
  • C to T, chromosome 13 at 24,911,653 bp
  • A to G, chromosome 13 at 69,619,541 bp
  • G to T, chromosome 13 at 93,498,925 bp
  • T to C, chromosome 13 at 111,768,494 bp
  • T to C, chromosome 13 at 118,386,840 bp
  • T to C, chromosome 14 at 47,551,345 bp
  • T to C, chromosome 14 at 54,569,526 bp
  • A to G, chromosome 14 at 55,073,534 bp
  • A to T, chromosome 14 at 110,751,388 bp
  • T to A, chromosome 14 at 123,599,913 bp
  • C to T, chromosome 15 at 83,620,069 bp
  • T to C, chromosome 16 at 22,011,554 bp
  • T to C, chromosome 16 at 57,506,768 bp
  • A to G, chromosome 18 at 34,632,038 bp
  • A to T, chromosome 19 at 5,654,089 bp
  • C to T, chromosome X at 70,394,529 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4231 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041050-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.