Strain Name:
C57BL/6J-MtgxR4244Btlr/Mmmh
Stock Number:
041060-MU
Citation ID:
RRID:MMRRC_041060-MU
Other Names:
R4244 (G1), C57BL/6J-MtgxR4244Btlr
Major Collection:

Strain Information

Dock5
Name: dedicator of cytokinesis 5
Synonyms: lr2, 1110060D06Rik, rlc
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 68813
VEGA: 14
Homologene: 57016
Ptpn5
Name: protein tyrosine phosphatase, non-receptor type 5
Synonyms: Step
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 19259
HGNC: HGNC:9657
Homologene: 8423
Tbcd
Name: tubulin-specific chaperone d
Synonyms: A030005L14Rik, 2310057L06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 108903
Homologene: 4368
Ccdc18
Name: coiled-coil domain containing 18
Synonyms: 1700021E15Rik, 4932411G06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 73254
Homologene: 35455
Copa
Name: coatomer protein complex subunit alpha
Synonyms: xenin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 12847
HGNC: HGNC:2230
Homologene: 3218
Ap1g1
Name: adaptor protein complex AP-1, gamma 1 subunit
Synonyms: gamma-adaptin, Adtg, D8Ertd374e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 11765
HGNC: HGNC:555
Homologene: 47995
Grm4
Name: glutamate receptor, metabotropic 4
Synonyms: mGluR4, Gprc1d
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 268934
HGNC: HGNC:4596
Homologene: 20230
Rfx7
Name: regulatory factor X, 7
Synonyms: 2510005N23Rik, 9930116O05Rik, D130086K05Rik, Rfxdc2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 319758
Homologene: 11275
Kcnv1
Name: potassium channel, subfamily V, member 1
Synonyms: 2700023A03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 67498
VEGA: 15
Homologene: 22811
Scn7a
Name: sodium channel, voltage-gated, type VII, alpha
Synonyms: Nav2.3, NaG, Nav2, 1110034K09Rik, Scn6a, Nax
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 20272
Homologene: 55706
Nav3
Name: neuron navigator 3
Synonyms: Pomfil1p, POMFIL1, 4732483H20Rik, unc53H3, steerin 3, 9630020C08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 260315
Homologene: 56688
Dock10
Name: dedicator of cytokinesis 10
Synonyms: Jr5, Jr4, ZIZ3, 9330153B10Rik, A630054M16Rik, Zizimin3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 210293
Homologene: 45952
Snx25
Name: sorting nexin 25
Synonyms: LOC382008, SBBI31
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 102141
Homologene: 23744
Cyld
Name: CYLD lysine 63 deubiquitinase
Synonyms: CYLD1, 2010013M14Rik, C130039D01Rik, 2900009M21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 74256
HGNC: HGNC:2584
Homologene: 9069
Map3k6
Name: mitogen-activated protein kinase kinase kinase 6
Synonyms: Ask2, MEKK6, MAPKKK6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 53608
HGNC: HGNC:6858
Homologene: 3435
Vwa5a
Name: von Willebrand factor A domain containing 5A
Synonyms: BCSC-1, 5830475I06Rik, Loh11cr2a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 67776
HGNC: HGNC:6658
Homologene: 18222
Cdh20
Name: cadherin 20
Synonyms: Cdh7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 23836
HGNC: HGNC:1760
Homologene: 8015
Slc27a1
Name: solute carrier family 27 (fatty acid transporter), member 1
Synonyms: FATP1, Fatp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 26457
Homologene: 8063
Cfap20dc
Name: CFAP20 domain containing
Synonyms: 4930452B06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 74430
Homologene: 18873
Slc5a5
Name: solute carrier family 5 (sodium iodide symporter), member 5
Synonyms: NIS
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 114479
Homologene: 37311
Zscan29
Name: zinc finger SCAN domains 29
Synonyms: Zfp690
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 99334
Homologene: 17586
Ccrl2
Name: chemokine (C-C motif) receptor-like 2
Synonyms: 1810047I05Rik, L-CCR, CCR11, Cmkbr1l2, Ackr5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 54199
HGNC: HGNC:1612
Homologene: 2948
Spaca9
Name: sperm acrosome associated 9
Synonyms: Mast, 1700026L06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 69987
HGNC: HGNC:1367
Homologene: 10346
Efcab3
Name: EF-hand calcium binding domain 3
Synonyms: 4921510J17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 70894
Homologene: 18304
Ehf
Name: ets homologous factor
Synonyms: 9030625L19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 13661
HGNC: HGNC:3246
Homologene: 7301
Or2ak6
Name: olfactory receptor family 2 subfamily AK member 6
Synonyms: GA_x6K02T2NKPP-708319-707399, MOR285-2, Olfr319
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 258493
Zar1
Name: zygote arrest 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 317755
Homologene: 18370
Gm9892
Name: predicted gene 9892
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 100040220
Or2y3
Name: olfactory receptor family 2 subfamily Y member 3
Synonyms: MOR256-4, GA_x6K02T2PSCP-2531299-2530355, Olfr131
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 258867
Homologene: 105889
Thnsl1
Name: threonine synthase-like 1 (bacterial)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 208967
Homologene: 32609
Sh3d21
Name: SH3 domain containing 21
Synonyms: 1700029G01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 66938
Homologene: 12057
Rab3gap1
Name: RAB3 GTPase activating protein subunit 1
Synonyms: 1700003B17Rik, 4732493F09Rik, p130
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 226407
Homologene: 45617
Vmn1r79
Name: vomeronasal 1 receptor 79
Synonyms: Gm9807
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 100042437
Homologene: 128342
Gm5174
Name: predicted gene 5174
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 382395
VEGA: 10
Homologene: 141143
Krt28
Name: keratin 28
Synonyms: 4733401L19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 70843
Homologene: 124711
Ccdc137
Name: coiled-coil domain containing 137
Synonyms: 3110023B02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 67291
Homologene: 34992
Actr1b
Name: ARP1 actin-related protein 1B, centractin beta
Synonyms: Arp1b, 2310066K23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 226977
HGNC: HGNC:168
Homologene: 101541
Sp5
Name: trans-acting transcription factor 5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 64406
Homologene: 11190
Or5d39
Name: olfactory receptor family 5 subfamily D member 39
Synonyms: GA_x6K02T2Q125-49641892-49640942, MOR174-16, Olfr1167
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258291
Homologene: 138307
D10Bwg1379e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 36,701,830 bp
  • T to A, chromosome 1 at 80,566,755 bp
  • C to T, chromosome 1 at 104,942,143 bp
  • T to C, chromosome 1 at 127,937,567 bp
  • A to G, chromosome 1 at 172,110,718 bp
  • A to G, chromosome 2 at 21,212,248 bp
  • T to C, chromosome 2 at 28,692,986 bp
  • T to C, chromosome 2 at 66,742,001 bp
  • T to C, chromosome 2 at 70,477,038 bp
  • T to C, chromosome 2 at 88,149,288 bp
  • G to A, chromosome 2 at 103,279,627 bp
  • C to T, chromosome 2 at 121,164,794 bp
  • C to T, chromosome 4 at 126,150,718 bp
  • T to C, chromosome 4 at 133,251,947 bp
  • T to C, chromosome 5 at 72,580,393 bp
  • C to T, chromosome 5 at 108,148,972 bp
  • T to A, chromosome 7 at 12,177,044 bp
  • C to T, chromosome 7 at 47,091,548 bp
  • A to G, chromosome 8 at 46,105,254 bp
  • A to T, chromosome 8 at 52,196,400 bp
  • A to G, chromosome 8 at 70,890,286 bp
  • A to G, chromosome 8 at 71,584,973 bp
  • A to T, chromosome 8 at 88,730,755 bp
  • G to A, chromosome 8 at 109,833,490 bp
  • T to C, chromosome 9 at 38,737,816 bp
  • A to G, chromosome 9 at 72,591,769 bp
  • T to C, chromosome 9 at 111,055,354 bp
  • C to A, chromosome 10 at 18,630,420 bp
  • C to A, chromosome 10 at 86,656,280 bp
  • T to C, chromosome 10 at 109,769,296 bp
  • T to G, chromosome 11 at 58,702,451 bp
  • G to T, chromosome 11 at 99,374,550 bp
  • A to T, chromosome 11 at 105,111,803 bp
  • T to C, chromosome 11 at 120,462,018 bp
  • T to A, chromosome 11 at 121,594,281 bp
  • T to C, chromosome 14 at 8,482,521 bp
  • A to G, chromosome 14 at 67,774,582 bp
  • G to A, chromosome 15 at 45,114,444 bp
  • A to G, chromosome 17 at 27,502,735 bp
  • C to T, chromosome 17 at 38,082,430 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4244 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041060-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.