Strain Name:
C57BL/6J-MtgxR4245Btlr/Mmmh
Stock Number:
041061-MU
Citation ID:
RRID:MMRRC_041061-MU
Other Names:
R4245 (G1), C57BL/6J-MtgxR4245Btlr
Major Collection:

Gene Information

Ptk2
Name: PTK2 protein tyrosine kinase 2
Synonyms: Fadk, FAK, FRNK
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 14083
VEGA: 15
HGNC: HGNC:9611
Homologene: 7314
Mthfd1
Name: methylenetetrahydrofolate dehydrogenase (NADP+ dependent), methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthase
Synonyms: DCS, E430024A07Rik, Mthfd
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 108156
VEGA: 12
HGNC: HGNC:7432
Homologene: 55940
Wdr59
Name: WD repeat domain 59
Synonyms: 5430401O09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 319481
Homologene: 38685
Zfp985
Name: zinc finger protein 985
Synonyms: Gm13154
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 433804
Homologene: 133076
Tg
Name: thyroglobulin
Synonyms: Tgn
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 21819
Homologene: 2430
Ndufs6
Name: NADH:ubiquinone oxidoreductase core subunit S6
Synonyms: IP13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 407785
HGNC: HGNC:7713
Homologene: 37935
Cdc23
Name: CDC23 cell division cycle 23
Synonyms: D18Ertd243e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 52563
HGNC: HGNC:1724
Homologene: 3426
Ccdc171
Name: coiled-coil domain containing 171
Synonyms: A330015D16Rik, 4930473A06Rik, 4930418J05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 320226
Homologene: 27942
Rfx7
Name: regulatory factor X, 7
Synonyms: 2510005N23Rik, Rfxdc2, 9930116O05Rik, D130086K05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 319758
Homologene: 11275
Dusp27
Name: dual specificity phosphatase 27 (putative)
Synonyms: C130085G02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 240892
Homologene: 18949
Ccdc184
Name: coiled-coil domain containing 184
Synonyms: AI836003
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 239650
VEGA: 15
Homologene: 18612
Sf3a1
Name: splicing factor 3a, subunit 1
Synonyms: 1200014H24Rik, 5930416L09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 67465
Homologene: 4294
Fhdc1
Name: FH2 domain containing 1
Synonyms: 6330505N24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229474
Homologene: 18920
Dnah5
Name: dynein, axonemal, heavy chain 5
Synonyms: b2b1154Clo, Mdnah5, b2b1565Clo, b2b1537Clo, Dnahc5, b2b3491Clo, b2b1134Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 110082
HGNC: HGNC:2950
Homologene: 1048
Muc4
Name: mucin 4
Synonyms: 4933405I11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 140474
HGNC: HGNC:7514
Homologene: 124469
Acacb
Name: acetyl-Coenzyme A carboxylase beta
Synonyms: Acc2, Accb
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 100705
HGNC: HGNC:85
Homologene: 74382
Scn7a
Name: sodium channel, voltage-gated, type VII, alpha
Synonyms: Nax, NaG, Nav2, Scn6a, Nav2.3, 1110034K09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 20272
Homologene: 55706
Dock10
Name: dedicator of cytokinesis 10
Synonyms: ZIZ3, Jr5, Jr4, 9330153B10Rik, A630054M16Rik, Zizimin3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 210293
Homologene: 45952
4931408C20Rik
Name: RIKEN cDNA 4931408C20 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 210940
Homologene: 86827
Jakmip2
Name: janus kinase and microtubule interacting protein 2
Synonyms: 6430702L21Rik, D930046L20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 76217
VEGA: 18
Homologene: 8866
C8a
Name: complement component 8, alpha polypeptide
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230558
HGNC: HGNC:1352
Homologene: 472
Vmn2r79
Name: vomeronasal 2, receptor 79
Synonyms: EG621430
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 621430
Homologene: 115466
Map3k6
Name: mitogen-activated protein kinase kinase kinase 6
Synonyms: Ask2, MEKK6, MAPKKK6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 53608
HGNC: HGNC:6858
Homologene: 3435
Mpeg1
Name: macrophage expressed gene 1
Synonyms: Mpg-1, MPS1, Perforin-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 17476
VEGA: 19
Homologene: 11216
Olfr313
Name: olfactory receptor 313
Synonyms: MOR222-2, GA_x6K02T2NKPP-590035-589109
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 258529
Homologene: 77375
Myo18b
Name: myosin XVIIIb
Synonyms: 4933411E19Rik, 4932408L24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 74376
Homologene: 53435
Zscan29
Name: zinc finger SCAN domains 29
Synonyms: Zfp690
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 99334
Homologene: 17586
Vill
Name: villin-like
Synonyms: Villp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 22351
Homologene: 22650
Rab3il1
Name: RAB3A interacting protein (rabin3)-like 1
Synonyms: 1200014K04Rik, Rab3ail1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 74760
HGNC: HGNC:9780
Homologene: 40880
Olfr18
Name: olfactory receptor 18
Synonyms: MOR145-1, MTPCR34, GA_x6K02T2PVTD-14054886-14053957
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 18315
Homologene: 138313
Radil
Name: Ras association and DIL domains
Synonyms: D930005D10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231858
Homologene: 77648
Ehf
Name: ets homologous factor
Synonyms: 9030625L19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 13661
HGNC: HGNC:3246
Homologene: 7301
Zar1
Name: zygote arrest 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 317755
Homologene: 18370
Thnsl1
Name: threonine synthase-like 1 (bacterial)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 208967
Homologene: 32609
Eepd1
Name: endonuclease/exonuclease/phosphatase family domain containing 1
Synonyms: 2310005P05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 67484
Homologene: 12144
Ptgir
Name: prostaglandin I receptor (IP)
Synonyms: IP, prostacyclin receptor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 19222
HGNC: HGNC:9602
Homologene: 7496
Actr1b
Name: ARP1 actin-related protein 1B, centractin beta
Synonyms: 2310066K23Rik, Arp1b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 226977
HGNC: HGNC:168
Homologene: 101541
Phactr3
Name: phosphatase and actin regulator 3
Synonyms: 1500003N10Rik, scapinin, 4930415A02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 74189
Homologene: 14482
Pcdhga1
Name: protocadherin gamma subfamily A, 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 93709
HGNC: HGNC:8696
Homologene: 56824
Gm17690
Name: predicted gene, 17690
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
4930449A18Rik
Name: RIKEN cDNA 4930449A18 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Gm5493
Name: predicted gene 5493
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Gm10143
Name: predicted gene 10143
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 26,682,080 bp
  • T to C, chromosome 1 at 36,701,830 bp
  • T to A, chromosome 1 at 80,566,755 bp
  • T to C, chromosome 1 at 166,101,116 bp
  • A to G, chromosome 2 at 21,212,248 bp
  • T to C, chromosome 2 at 66,742,001 bp
  • G to A, chromosome 2 at 103,279,627 bp
  • C to T, chromosome 2 at 121,164,794 bp
  • G to A, chromosome 2 at 178,283,189 bp
  • T to A, chromosome 3 at 59,825,782 bp
  • C to A, chromosome 3 at 84,444,826 bp
  • T to A, chromosome 3 at 96,168,077 bp
  • A to G, chromosome 4 at 83,554,808 bp
  • A to G, chromosome 4 at 104,876,346 bp
  • T to C, chromosome 4 at 133,251,947 bp
  • A to G, chromosome 4 at 147,582,939 bp
  • T to C, chromosome 5 at 72,580,393 bp
  • G to A, chromosome 5 at 112,692,395 bp
  • A to T, chromosome 5 at 114,230,784 bp
  • T to C, chromosome 5 at 142,543,791 bp
  • A to G, chromosome 7 at 16,906,869 bp
  • A to G, chromosome 7 at 87,002,416 bp
  • T to C, chromosome 8 at 111,490,364 bp
  • T to C, chromosome 9 at 20,314,333 bp
  • T to C, chromosome 9 at 25,594,624 bp
  • A to G, chromosome 9 at 72,591,769 bp
  • T to A, chromosome 9 at 119,071,291 bp
  • C to T, chromosome 11 at 4,167,774 bp
  • T to C, chromosome 11 at 58,817,778 bp
  • T to C, chromosome 12 at 76,301,273 bp
  • A to T, chromosome 13 at 73,320,393 bp
  • A to G, chromosome 15 at 28,219,189 bp
  • T to C, chromosome 15 at 66,696,469 bp
  • C to T, chromosome 15 at 73,231,976 bp
  • T to A, chromosome 15 at 98,168,869 bp
  • T to C, chromosome 16 at 32,753,802 bp
  • A to G, chromosome 17 at 22,747,226 bp
  • T to C, chromosome 18 at 34,637,047 bp
  • T to A, chromosome 18 at 37,663,552 bp
  • A to G, chromosome 18 at 43,577,436 bp
  • A to T, chromosome 19 at 10,030,154 bp
  • T to A, chromosome 19 at 10,200,367 bp
  • C to A, chromosome 19 at 12,462,908 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4245 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041061-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.