Strain Name:
C57BL/6J-MtgxR4259Btlr/Mmmh
Stock Number:
041072-MU
Citation ID:
RRID:MMRRC_041072-MU
Other Names:
R4259 (G1), C57BL/6J-MtgxR4259Btlr
Major Collection:

Gene Information

Cep290
Name: centrosomal protein 290
Synonyms: Nphp6, b2b1454Clo, b2b1752Clo, Kiaa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 216274
VEGA: 10
Homologene: 77213
Actr10
Name: ARP10 actin-related protein 10
Synonyms: Arp11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 56444
VEGA: 12
Homologene: 10220
Pdgfrb
Name: platelet derived growth factor receptor, beta polypeptide
Synonyms: Pdgfr, CD140b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 18596
HGNC: HGNC:8804
Homologene: 1960
Lama1
Name: laminin, alpha 1
Synonyms: Lama
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 16772
VEGA: 17
HGNC: HGNC:6481
Homologene: 21146
Paf1
Name: Paf1, RNA polymerase II complex component
Synonyms: 5730511K23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 54624
Homologene: 5441
Tbc1d32
Name: TBC1 domain family, member 32
Synonyms: Bromi, C6orf170, b2b2284Clo, D630037F22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 544696
VEGA: 10
Homologene: 51889
Itpr3
Name: inositol 1,4,5-triphosphate receptor 3
Synonyms: Itpr-3, Ip3r3, tf
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 16440
HGNC: HGNC:6182
Homologene: 1675
Pgk2
Name: phosphoglycerate kinase 2
Synonyms: Tcp-2, Pgk-2, Tcp-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 18663
VEGA: 17
HGNC: HGNC:8898
Homologene: 57138
Dnah12
Name: dynein, axonemal, heavy chain 12
Synonyms: Hdhc3, Dnahc7l, LOC380889, HL-19, DLP12, HL19, DHC3, 4921531P07Rik, Dnahc12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 110083
HGNC: HGNC:2943
Homologene: 56821
Slc38a4
Name: solute carrier family 38, member 4
Synonyms: SNAT4, 1700012A18Rik, 1110012E16Rik, Ata3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 69354
VEGA: 15
Homologene: 75077
1700010I14Rik
Name: RIKEN cDNA 1700010I14 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 66931
Homologene: 49841
Col9a3
Name: collagen, type IX, alpha 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 12841
HGNC: HGNC:2219
Homologene: 20438
Cyp2b19
Name: cytochrome P450, family 2, subfamily b, polypeptide 19
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 13090
Homologene: 104114
Kmt2b
Name: lysine (K)-specific methyltransferase 2B
Synonyms: 2610014H22Rik, Wbp7, Mll2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 75410
Homologene: 22838
4930407I10Rik
Name: RIKEN cDNA 4930407I10 gene
Synonyms: LOC328573
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 328573
VEGA: 15
Homologene: 130065
Krt72
Name: keratin 72
Synonyms: Krt72-ps, K6irs2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 105866
Homologene: 25876
Pdk2
Name: pyruvate dehydrogenase kinase, isoenzyme 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 18604
HGNC: HGNC:8810
Homologene: 68265
Lrrk1
Name: leucine-rich repeat kinase 1
Synonyms: C230002E15Rik, D130026O16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233328
Homologene: 23464
Pde6c
Name: phosphodiesterase 6C, cGMP specific, cone, alpha prime
Synonyms: cpfl1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 110855
VEGA: 19
HGNC: HGNC:8787
Homologene: 4521
Cd248
Name: CD248 antigen, endosialin
Synonyms: 2610111G01Rik, Cd164l1, Tem1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 70445
VEGA: 19
Homologene: 10699
Sgsm2
Name: small G protein signaling modulator 2
Synonyms: D630003G22Rik, Rutbc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 97761
Homologene: 14780
Slc5a4b
Name: solute carrier family 5 (neutral amino acid transporters, system A), member 4b
Synonyms: 2010104G07Rik, SAAT1, pSGLT2, SGLT3b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 64454
Homologene: 56968
Llgl1
Name: LLGL1 scribble cell polarity complex component
Synonyms: Lgl1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 16897
HGNC: HGNC:6628
Homologene: 31220
Olfr1076
Name: olfactory receptor 1076
Synonyms: MOR189-2, GA_x6K02T2Q125-47993761-47994702
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258401
Homologene: 17233
Prkn
Name: parkin RBR E3 ubiquitin protein ligase
Synonyms: Park2, Parkin, PRKN
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 50873
HGNC: HGNC:8607
Homologene: 3355
Fam71f1
Name: family with sequence similarity 71, member F1
Synonyms: LOC330277
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 330277
Homologene: 13077
Hcn1
Name: hyperpolarization activated cyclic nucleotide gated potassium channel 1
Synonyms: hyperpolarization-activated, cyclic nucleotide-gated K+ 1, C630013B14Rik, Bcng1, HAC2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 15165
HGNC: HGNC:4845
Homologene: 32093
Olfr480
Name: olfactory receptor 480
Synonyms: GA_x6K02T2PBJ9-10395807-10394869, MOR204-32
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 56861
Homologene: 72039
Slc26a1
Name: solute carrier family 26 (sulfate transporter), member 1
Synonyms: Sat1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231583
Homologene: 32539
Prl3b1
Name: prolactin family 3, subfamily b, member 1
Synonyms: Pl-2, PL, Csh2, mplII, Pl2, mPL-II, prolactin-like
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 18776
Homologene: 7476
Olfr150
Name: olfactory receptor 150
Synonyms: GA_x6K02T2PVTD-33434302-33435240, M93, MOR171-18
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 258602
VEGA: 9
Gpr171
Name: G protein-coupled receptor 171
Synonyms: H963, F730001G15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229323
Homologene: 36330
Igkv4-90
Name: immunoglobulin kappa chain variable 4-90
Synonyms: Gm16906
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 434034
Gm4975
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Gm20394
Name: predicted gene 20394
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Sat1
Name: spermidine/spermine N1-acetyl transferase 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 20229
Homologene: 37716
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 2 at 86,508,999 bp
  • A to G, chromosome 2 at 180,610,228 bp
  • A to G, chromosome 3 at 59,097,527 bp
  • T to A, chromosome 5 at 108,672,630 bp
  • C to G, chromosome 6 at 29,320,801 bp
  • T to C, chromosome 6 at 68,807,477 bp
  • G to A, chromosome 7 at 26,763,382 bp
  • T to C, chromosome 7 at 28,395,807 bp
  • T to C, chromosome 7 at 30,581,081 bp
  • A to T, chromosome 7 at 66,330,764 bp
  • T to A, chromosome 7 at 108,065,893 bp
  • A to T, chromosome 8 at 63,928,296 bp
  • A to T, chromosome 9 at 39,737,703 bp
  • A to G, chromosome 10 at 56,049,771 bp
  • A to G, chromosome 10 at 76,103,852 bp
  • A to C, chromosome 10 at 100,514,492 bp
  • C to T, chromosome 11 at 60,709,568 bp
  • T to A, chromosome 11 at 74,892,028 bp
  • T to C, chromosome 11 at 95,041,144 bp
  • C to A, chromosome 11 at 100,351,842 bp
  • T to C, chromosome 12 at 70,952,985 bp
  • T to C, chromosome 13 at 27,243,906 bp
  • A to T, chromosome 13 at 117,975,348 bp
  • A to G, chromosome 14 at 26,798,926 bp
  • A to G, chromosome 15 at 82,063,726 bp
  • A to G, chromosome 15 at 96,998,493 bp
  • T to C, chromosome 15 at 101,778,257 bp
  • T to C, chromosome 17 at 8,995,234 bp
  • T to C, chromosome 17 at 11,854,795 bp
  • T to A, chromosome 17 at 27,106,324 bp
  • T to G, chromosome 17 at 40,207,383 bp
  • T to C, chromosome 17 at 67,752,418 bp
  • A to C, chromosome 18 at 61,077,631 bp
  • A to G, chromosome 19 at 5,068,838 bp
  • G to A, chromosome 19 at 38,162,845 bp
  • T to C, chromosome X at 155,215,186 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4259 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041072-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.