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Strain Name:
C57BL/6J-MtgxR4299Btlr/Mmmh
Stock Number:
041087-MU
Citation ID:
RRID:MMRRC_041087-MU
Other Names:
R4299 (G1), C57BL/6J-MtgxR4299Btlr
Major Collection:

Strain Information

Myh9
Name: myosin, heavy polypeptide 9, non-muscle
Synonyms: D0Jmb2, E030044M24Rik, NMHC II-A, Myhn-1, Myhn1, myosin IIA, Fltn
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 17886
HGNC: HGNC:7579
Homologene: 129835
Rgs14
Name: regulator of G-protein signaling 14
Synonyms: Rap1/rap2 interacting protein
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 51791
HGNC: HGNC:9996
Homologene: 4735
Macf1
Name: microtubule-actin crosslinking factor 1
Synonyms: Acf7, Aclp7, trabeculin alpha
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11426
Homologene: 136191
Cbfa2t2
Name: CBFA2/RUNX1 translocation partner 2
Synonyms: MTGR1, C330013D05Rik, Cbfa2t2h
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 12396
HGNC: HGNC:1536
Homologene: 3733
Clasp1
Name: CLIP associating protein 1
Synonyms: 5730583A19Rik, 1700030C23Rik, mCLASP1, CLASP1alpha, CLASP1, B130045P17Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 76707
Homologene: 41024
Kmt2e
Name: lysine (K)-specific methyltransferase 2E
Synonyms: 1810033J14Rik, D230038D11Rik, 9530077A04Rik, Mll5
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 69188
Homologene: 18822
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 72,394,720 bp
  • T to C, chromosome 1 at 110,061,001 bp
  • C to T, chromosome 1 at 118,459,854 bp
  • C to A, chromosome 1 at 138,283,724 bp
  • T to A, chromosome 1 at 156,705,799 bp
  • T to A, chromosome 1 at 174,152,312 bp
  • C to A, chromosome 2 at 4,333,071 bp
  • A to T, chromosome 2 at 30,285,188 bp
  • A to T, chromosome 2 at 86,298,241 bp
  • A to G, chromosome 2 at 91,169,803 bp
  • G to A, chromosome 2 at 150,190,733 bp
  • G to A, chromosome 2 at 154,523,928 bp
  • G to T, chromosome 3 at 133,367,842 bp
  • C to T, chromosome 3 at 146,068,806 bp
  • A to G, chromosome 4 at 11,597,865 bp
  • C to T, chromosome 4 at 52,440,238 bp
  • A to T, chromosome 4 at 63,398,032 bp
  • T to C, chromosome 4 at 88,628,182 bp
  • C to A, chromosome 4 at 98,677,321 bp
  • A to G, chromosome 4 at 118,365,406 bp
  • C to G, chromosome 4 at 121,054,258 bp
  • A to G, chromosome 4 at 123,399,406 bp
  • A to C, chromosome 5 at 21,920,487 bp
  • T to A, chromosome 5 at 23,464,914 bp
  • T to C, chromosome 5 at 31,250,599 bp
  • A to G, chromosome 5 at 31,487,526 bp
  • A to T, chromosome 5 at 66,313,378 bp
  • T to C, chromosome 5 at 73,666,902 bp
  • C to A, chromosome 5 at 109,091,964 bp
  • A to G, chromosome 5 at 124,819,925 bp
  • T to C, chromosome 5 at 139,709,471 bp
  • T to C, chromosome 5 at 145,573,677 bp
  • G to T, chromosome 5 at 147,683,907 bp
  • A to G, chromosome 6 at 84,068,077 bp
  • C to T, chromosome 7 at 17,095,233 bp
  • A to T, chromosome 7 at 31,044,982 bp
  • A to G, chromosome 7 at 81,328,035 bp
  • T to A, chromosome 7 at 103,787,995 bp
  • G to A, chromosome 7 at 118,984,214 bp
  • T to C, chromosome 7 at 140,652,243 bp
  • T to C, chromosome 8 at 86,531,525 bp
  • T to C, chromosome 8 at 110,962,359 bp
  • A to G, chromosome 9 at 27,052,327 bp
  • A to G, chromosome 9 at 36,763,344 bp
  • T to G, chromosome 9 at 38,430,812 bp
  • T to A, chromosome 9 at 38,975,759 bp
  • T to C, chromosome 9 at 39,144,999 bp
  • T to C, chromosome 9 at 39,592,979 bp
  • A to G, chromosome 9 at 64,155,162 bp
  • G to A, chromosome 9 at 107,257,449 bp
  • T to G, chromosome 9 at 119,765,506 bp
  • C to A, chromosome 10 at 121,745,446 bp
  • C to G, chromosome 11 at 61,140,817 bp
  • A to T, chromosome 11 at 67,055,800 bp
  • A to C, chromosome 11 at 69,909,061 bp
  • A to T, chromosome 11 at 73,454,001 bp
  • T to C, chromosome 12 at 112,739,305 bp
  • A to G, chromosome 13 at 55,383,753 bp
  • T to A, chromosome 14 at 58,893,523 bp
  • T to A, chromosome 14 at 62,925,140 bp
  • T to C, chromosome 15 at 54,252,095 bp
  • T to C, chromosome 15 at 77,769,964 bp
  • T to C, chromosome 15 at 100,796,640 bp
  • T to C, chromosome 16 at 20,107,004 bp
  • G to A, chromosome 16 at 22,805,411 bp
  • A to T, chromosome 17 at 21,229,021 bp
  • A to T, chromosome 17 at 25,115,256 bp
  • T to C, chromosome 17 at 34,731,144 bp
  • C to T, chromosome 17 at 37,055,900 bp
  • T to A, chromosome 18 at 20,595,951 bp
  • C to G, chromosome 18 at 67,088,583 bp
  • G to A, chromosome 18 at 82,987,497 bp
  • A to T, chromosome 18 at 84,094,501 bp
  • C to T, chromosome 19 at 6,049,921 bp
  • T to C, chromosome 19 at 40,183,928 bp
  • A to T, chromosome X at 87,300,707 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4299 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041087-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.