Strain Name:
C57BL/6J-MtgxR4328Btlr/Mmmh
Stock Number:
041098-MU
Citation ID:
RRID:MMRRC_041098-MU
Other Names:
R4328 (G1), C57BL/6J-MtgxR4328Btlr
Major Collection:

Strain Information

Hcn2
Name: hyperpolarization-activated, cyclic nucleotide-gated K+ 2
Synonyms: HAC1, trls
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 15166
HGNC: HGNC:4846
Homologene: 31022
Crym
Name: crystallin, mu
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 12971
HGNC: HGNC:2418
Homologene: 1424
Cwf19l2
Name: CWF19-like 2, cell cycle control (S. pombe)
Synonyms: 3230401L03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 244672
Homologene: 12366
Nup210l
Name: nucleoporin 210-like
Synonyms: 4930548O11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 77595
Homologene: 28122
Hira
Name: histone cell cycle regulator
Synonyms: Tuple1, D16Ertd95e, Gm15797
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 15260
HGNC: HGNC:4916
Homologene: 48172
Prep
Name: prolyl endopeptidase
Synonyms: prolyl oligopeptidase, D10Wsu136e, Pop
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 19072
VEGA: 10
HGNC: HGNC:9358
Homologene: 2042
Snrnp200
Name: small nuclear ribonucleoprotein 200 (U5)
Synonyms: U5-200KD, HELIC2, A330064G03Rik, Ascc3l1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 320632
Homologene: 5859
Pafah1b1
Name: platelet-activating factor acetylhydrolase, isoform 1b, subunit 1
Synonyms: Lis1, LIS-1, lissencephaly-1 protein, Mdsh, Pafaha, PAF-AH 45
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 18472
HGNC: HGNC:8574
Homologene: 371
Rabgap1
Name: RAB GTPase activating protein 1
Synonyms: Gapcena
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 227800
Homologene: 49301
Arap2
Name: ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 2
Synonyms: Centd1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 212285
Homologene: 9064
Palm
Name: paralemmin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 18483
VEGA: 10
HGNC: HGNC:8594
Homologene: 1937
Cd55
Name: CD55 molecule, decay accelerating factor for complement
Synonyms: Daf-GPI, complement-glycosylphosphatidylinositol, GPI-DAF, Cromer blood group, Daf1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 13136
HGNC: HGNC:2665
Homologene: 479
Twsg1
Name: twisted gastrulation BMP signaling modulator 1
Synonyms: 9030422N06Rik, Tsg, D17Ertd403e, 1810013J15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 65960
VEGA: 17
Homologene: 10774
Gm3604
Name: predicted gene 3604
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 100041979
Homologene: 136271
Ppp3cb
Name: protein phosphatase 3, catalytic subunit, beta isoform
Synonyms: PP2BA beta, Calnb, CnAbeta, 1110063J16Rik, Cnab
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 19056
HGNC: HGNC:9315
Homologene: 56429
Kirrel
Name: kirre like nephrin family adhesion molecule 1
Synonyms: Neph1, Kirrel1, 6720469N11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 170643
Homologene: 10089
Gskip
Name: GSK3B interacting protein
Synonyms: 4933433P14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 66787
VEGA: 12
Homologene: 9522
Tmem107
Name: transmembrane protein 107
Synonyms: 2810049P21Rik, 1110004B13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 66910
Homologene: 12052
Ttyh1
Name: tweety family member 1
Synonyms: tty, 6330408P11Rik, 4930459B04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 57776
Homologene: 10779
Ryr1
Name: ryanodine receptor 1, skeletal muscle
Synonyms: calcium release channel isoform 1, Ryr, skrr
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 20190
Homologene: 68069
Col13a1
Name: collagen, type XIII, alpha 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 12817
HGNC: HGNC:2190
Homologene: 22421
Med12l
Name: mediator complex subunit 12-like
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 329650
Homologene: 43143
Col12a1
Name: collagen, type XII, alpha 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 12816
HGNC: HGNC:2188
Homologene: 3217
Gpn2
Name: GPN-loop GTPase 2
Synonyms: Atpbd1b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 100210
Homologene: 7006
9930021J03Rik
Name: RIKEN cDNA 9930021J03 gene
Synonyms: Gm9832
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 240613
Homologene: 19046
Tmem62
Name: transmembrane protein 62
Synonyms: B830009D23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 96957
Homologene: 11801
Ppargc1b
Name: peroxisome proliferative activated receptor, gamma, coactivator 1 beta
Synonyms: PGC-1beta/ERRL1, 4631412G21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 170826
Homologene: 15776
Lingo2
Name: leucine rich repeat and Ig domain containing 2
Synonyms: B230217C06Rik, Lrrn6c, LERN3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 242384
Homologene: 17621
Aadacl4fm2
Name: AADACL4 family member 2
Synonyms: Gm13124
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 627085
Homologene: 135767
Niban1
Name: niban apoptosis regulator 1
Synonyms: Niban, Fam129a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 63913
Homologene: 62170
Alcam
Name: activated leukocyte cell adhesion molecule
Synonyms: DM-GRASP, CD166, MuSC, SC1, BEN
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 11658
HGNC: HGNC:400
Homologene: 1229
Ip6k2
Name: inositol hexaphosphate kinase 2
Synonyms: 1500005N04Rik, Ihpk2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 76500
Homologene: 56929
Or2t1
Name: olfactory receptor family 2 subfamily T member 1
Synonyms: MTPCR53, MOR274-1, GA_x6K02T2PLTE-6714644-6715597, Olfr31
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 18330
HGNC: HGNC:8277
Homologene: 74028
Tent5b
Name: terminal nucleotidyltransferase 5B
Synonyms: 4732473B16Rik, Fam46b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 100342
Homologene: 24928
Ro60
Name: Ro60, Y RNA binding protein
Synonyms: SS-A/Ro, Ssa, A530054J02Rik, 1810007I17Rik, Ssa2, Trove2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 20822
Homologene: 3383
Zfp184
Name: zinc finger protein 184 (Kruppel-like)
Synonyms: 4930500C15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 193452
Homologene: 113602
Pak3
Name: p21 (RAC1) activated kinase 3
Synonyms: PAK-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 18481
HGNC: HGNC:8592
Homologene: 55664
Bin3
Name: bridging integrator 3
Synonyms: 1700015F07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 57784
VEGA: 14
HGNC: HGNC:1054
Homologene: 5472
Klhl40
Name: kelch-like 40
Synonyms: 2310024D23Rik, Kbtbd5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 72330
VEGA: 9
Homologene: 17571
Or7g34
Name: olfactory receptor family 7 subfamily G member 34
Synonyms: GA_x6K02T2PVTD-13313295-13312357, MOR147-3, Olfr854
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 258515
Homologene: 128144
Or5w12
Name: olfactory receptor family 5 subfamily W member 12
Synonyms: GA_x6K02T2Q125-49177531-49176599, MOR177-2, Olfr1135
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258654
Homologene: 17331
Or8d1
Name: olfactory receptor family 8 subfamily D member 1
Synonyms: MTPCR09, MOR171-9, GA_x6K02T2PVTD-32550930-32551856, Olfr26
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 18324
VEGA: 9
HGNC: HGNC:8481
Homologene: 79676
Erlec1
Name: endoplasmic reticulum lectin 1
Synonyms: 4933407N01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 66753
Homologene: 9247
Zpbp2
Name: zona pellucida binding protein 2
Synonyms: 1700017D11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 69376
Homologene: 18892
Ly6e
Name: lymphocyte antigen 6 complex, locus E
Synonyms: Sca-2, Tsa1, Ly67, 9804, RIG-E, TSA-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 17069
HGNC: HGNC:6727
Homologene: 56411
Med24
Name: mediator complex subunit 24
Synonyms: Trap100, D11Ertd307e, R75526, 100kDa, DRIP100, Gse2, Pparb2, Thrap4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 23989
Homologene: 40795
Prickle4
Name: prickle planar cell polarity protein 4
Synonyms: LOC381104
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 381104
Homologene: 22752
Pax8
Name: paired box 8
Synonyms: Pax-8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 18510
HGNC: HGNC:8622
Homologene: 2589
Zfp239
Name: zinc finger protein 239
Synonyms: Mok-2, Mok2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 22685
Homologene: 68480
Gm3336
Name: predicted gene 3336
Synonyms: 2410018E23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 100502950
Homologene: 136417
Gm12367
Name: predicted gene 12367
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 118568669
2300009A05Rik
Name: RIKEN cDNA 2300009A05 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 69478
Homologene: 19058
Eif1ad16
Name: eukaryotic translation initiation factor 1A domain containing 16
Synonyms: Gm6803
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 627873
VEGA: 12
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 130,447,367 bp
  • A to T, chromosome 1 at 130,452,483 bp
  • G to A, chromosome 1 at 143,766,707 bp
  • T to C, chromosome 1 at 151,636,418 bp
  • A to G, chromosome 2 at 24,441,651 bp
  • T to A, chromosome 2 at 37,532,615 bp
  • C to A, chromosome 2 at 87,671,664 bp
  • T to A, chromosome 2 at 120,980,510 bp
  • T to A, chromosome 2 at 127,222,217 bp
  • T to C, chromosome 3 at 59,265,267 bp
  • T to C, chromosome 3 at 87,084,774 bp
  • C to A, chromosome 3 at 90,175,835 bp
  • T to A, chromosome 4 at 35,225,985 bp
  • T to A, chromosome 4 at 35,708,462 bp
  • C to T, chromosome 4 at 133,486,603 bp
  • T to C, chromosome 4 at 133,588,608 bp
  • T to A, chromosome 4 at 144,555,594 bp
  • A to T, chromosome 5 at 62,621,863 bp
  • T to C, chromosome 6 at 117,871,784 bp
  • A to T, chromosome 7 at 4,130,581 bp
  • T to C, chromosome 7 at 29,083,059 bp
  • C to T, chromosome 7 at 120,195,339 bp
  • C to T, chromosome 8 at 70,720,585 bp
  • A to T, chromosome 9 at 3,458,878 bp
  • T to A, chromosome 9 at 19,567,022 bp
  • T to C, chromosome 9 at 38,855,836 bp
  • T to C, chromosome 9 at 63,398,956 bp
  • T to A, chromosome 9 at 79,700,389 bp
  • G to A, chromosome 9 at 108,805,648 bp
  • A to G, chromosome 9 at 121,778,890 bp
  • T to C, chromosome 10 at 45,120,649 bp
  • T to C, chromosome 10 at 61,863,979 bp
  • T to C, chromosome 10 at 79,724,611 bp
  • G to A, chromosome 10 at 79,807,686 bp
  • C to T, chromosome 11 at 30,949,972 bp
  • G to T, chromosome 11 at 69,071,475 bp
  • T to C, chromosome 11 at 74,682,240 bp
  • A to G, chromosome 11 at 98,557,606 bp
  • A to G, chromosome 11 at 98,707,116 bp
  • T to A, chromosome 12 at 88,018,515 bp
  • T to C, chromosome 12 at 105,700,701 bp
  • T to C, chromosome 13 at 21,959,902 bp
  • A to T, chromosome 13 at 62,369,265 bp
  • T to A, chromosome 14 at 14,328,193 bp
  • A to T, chromosome 14 at 20,530,948 bp
  • A to G, chromosome 14 at 70,118,605 bp
  • C to A, chromosome 15 at 74,958,521 bp
  • C to A, chromosome 16 at 18,896,612 bp
  • T to C, chromosome 16 at 52,253,216 bp
  • C to A, chromosome 17 at 47,688,618 bp
  • T to C, chromosome 17 at 65,948,738 bp
  • A to T, chromosome 18 at 61,382,469 bp
  • G to T, chromosome 19 at 29,743,561 bp
  • T to C, chromosome X at 143,733,209 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4328 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041098-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.