Strain Name:
C57BL/6J-MtgxR4347Btlr/Mmmh
Stock Number:
041102-MU
Citation ID:
RRID:MMRRC_041102-MU
Other Names:
R4347 (G1), C57BL/6J-MtgxR4347Btlr
Major Collection:

Gene Information

Usp5
Name: ubiquitin specific peptidase 5 (isopeptidase T)
Synonyms: Ucht
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 22225
Homologene: 55758
Srek1
Name: splicing regulatory glutamine/lysine-rich protein 1
Synonyms: SRrp86, AL118220, Sfrs12, SRrp508
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 218543
Homologene: 10581
Kif1b
Name: kinesin family member 1B
Synonyms: KIF1Bp204, KIF1Bp130, A530096N05Rik, D4Mil1e, N-3 kinesin, Kif1b alpha, Kif1b beta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 16561
Homologene: 99835
Vim
Name: vimentin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 22352
Homologene: 2538
Syvn1
Name: synovial apoptosis inhibitor 1, synoviolin
Synonyms: 1200010C09Rik, Hrd1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 74126
Homologene: 32700
Gpihbp1
Name: GPI-anchored HDL-binding protein 1
Synonyms: 1110002J19Rik, GPI-HBP1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 68453
Homologene: 136164
Olfml1
Name: olfactomedin-like 1
Synonyms: ONT2, MVAL564, mONT2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 244198
Homologene: 45688
Efl1
Name: elongation factor like GTPase 1
Synonyms: 4932434J20Rik, D7Ertd791e, Eftud1, 6030468D11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 101592
Homologene: 11599
Arhgap28
Name: Rho GTPase activating protein 28
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 268970
Homologene: 18264
Plekhh2
Name: pleckstrin homology domain containing, family H (with MyTH4 domain) member 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 213556
VEGA: 17
Homologene: 35317
Kcnab1
Name: potassium voltage-gated channel, shaker-related subfamily, beta member 1
Synonyms: Akr8a8, Kvbeta1.1, mKv(beta)1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 16497
HGNC: HGNC:6228
Homologene: 56491
Nrp1
Name: neuropilin 1
Synonyms: NPN-1, Neuropilin-1, Npn1, NP-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 18186
HGNC: HGNC:8004
Homologene: 2876
Mprip
Name: myosin phosphatase Rho interacting protein
Synonyms: Rhoip3, Gm34094, p116 Rho interacting protein, RIP3, p116Rip
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 26936
Homologene: 9034
Ddx60
Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 60
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234311
Homologene: 23031
Abca5
Name: ATP-binding cassette, sub-family A (ABC1), member 5
Synonyms: ABC13, B930033A02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 217265
HGNC: HGNC:35
Homologene: 10263
4921536K21Rik
Name: RIKEN cDNA 4921536K21 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 67430
Homologene: 12129
Dvl3
Name: dishevelled segment polarity protein 3
Synonyms: b2b2866Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 13544
HGNC: HGNC:3087
Homologene: 20928
Slc25a54
Name: solute carrier family 25, member 54
Synonyms: SCaMC-1like, 4930443G12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 74686
Homologene: 82464
Prr27
Name: proline rich 27
Synonyms: 4930432K09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 73779
Homologene: 136277
Acox1
Name: acyl-Coenzyme A oxidase 1, palmitoyl
Synonyms: D130055E20Rik, Acyl-CoA oxidase, AOX
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 11430
HGNC: HGNC:119
Homologene: 38299
Gal3st2b
Name: galactose-3-O-sulfotransferase 2B
Synonyms: Gm9994
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 100041596
Homologene: 41471
Igkv13-84
Name: immunoglobulin kappa chain variable 13-84
Synonyms: Igk-V33
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 692152
Trim3
Name: tripartite motif-containing 3
Synonyms: Rnf22, BERP1, HAC1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 55992
Homologene: 21290
Chil4
Name: chitinase-like 4
Synonyms: Chi3l4, Ym2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 104183
Homologene: 74931
4930503E14Rik
Name: RIKEN cDNA 4930503E14 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 74954
Homologene: 115686
Gm7135
Name: predicted gene 7135
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 634346
Homologene: 86643
Igkv10-96
Name: immunoglobulin kappa variable 10-96
Synonyms: Gm16637
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 692165
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 93,939,808 bp
  • T to A, chromosome 1 at 97,348,310 bp
  • T to G, chromosome 2 at 13,575,518 bp
  • A to G, chromosome 3 at 65,297,475 bp
  • T to C, chromosome 3 at 106,202,828 bp
  • A to G, chromosome 3 at 109,102,739 bp
  • C to A, chromosome 4 at 149,247,234 bp
  • A to G, chromosome 5 at 87,842,672 bp
  • T to A, chromosome 6 at 68,632,180 bp
  • T to A, chromosome 6 at 68,939,776 bp
  • T to A, chromosome 6 at 124,821,195 bp
  • A to T, chromosome 7 at 82,697,966 bp
  • C to T, chromosome 7 at 105,619,387 bp
  • C to T, chromosome 7 at 107,567,833 bp
  • T to C, chromosome 8 at 62,024,973 bp
  • T to C, chromosome 8 at 128,480,991 bp
  • C to T, chromosome 11 at 3,890,122 bp
  • T to C, chromosome 11 at 59,759,453 bp
  • A to T, chromosome 11 at 110,299,968 bp
  • T to C, chromosome 11 at 116,198,661 bp
  • C to T, chromosome 13 at 103,748,759 bp
  • C to A, chromosome 14 at 44,171,178 bp
  • T to G, chromosome 15 at 75,598,168 bp
  • G to A, chromosome 16 at 20,531,299 bp
  • C to T, chromosome 17 at 67,873,142 bp
  • G to A, chromosome 17 at 84,619,702 bp
  • C to T, chromosome 19 at 6,049,921 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4347 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041102-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.