Strain Name:
C57BL/6J-MtgxR4374Btlr/Mmmh
Stock Number:
041118-MU
Citation ID:
RRID:MMRRC_041118-MU
Other Names:
R4374 (G1), C57BL/6J-MtgxR4374Btlr
Major Collection:

Strain Information

Frem2
Name: Fras1 related extracellular matrix protein 2
Synonyms: 8430406N05Rik, 6030440P17Rik, my, ne, b2b1562Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 242022
Homologene: 18454
Lrsam1
Name: leucine rich repeat and sterile alpha motif containing 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 227738
Homologene: 44526
Phf20l1
Name: PHD finger protein 20-like 1
Synonyms: CGI-72, E130113K22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 239510
VEGA: 15
Homologene: 9341
Osbpl8
Name: oxysterol binding protein-like 8
Synonyms: ORP-8, D330025H14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 237542
VEGA: 10
Homologene: 68813
Akap13
Name: A kinase (PRKA) anchor protein 13
Synonyms: AKAP-Lbc, PROTO-LBC, PROTO-LB, Ht31, 5730522G15Rik, 1700026G02Rik, 5830460E08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 75547
HGNC: HGNC:371
Homologene: 4903
Nsmaf
Name: neutral sphingomyelinase (N-SMase) activation associated factor
Synonyms: Fan, factor associated with N-SMase activation
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 18201
HGNC: HGNC:8017
Homologene: 2652
Cpsf7
Name: cleavage and polyadenylation specific factor 7
Synonyms: C330017N18Rik, 5730453I16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 269061
VEGA: 19
Homologene: 11731
Zdhhc13
Name: zinc finger, DHHC domain containing 13
Synonyms: kojak, 2410004E01Rik, Hip14l, skc4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 243983
Homologene: 75106
Sqle
Name: squalene epoxidase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 20775
Homologene: 2355
Myt1
Name: myelin transcription factor 1
Synonyms: NZF-2b, NZF-2a, Nzf2, Nztf2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 17932
HGNC: HGNC:7622
Homologene: 3332
Pole
Name: polymerase (DNA directed), epsilon
Synonyms: pol-epsilon
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 18973
HGNC: HGNC:9177
Homologene: 4539
Ercc1
Name: excision repair cross-complementing rodent repair deficiency, complementation group 1
Synonyms: Ercc-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 13870
HGNC: HGNC:3433
Homologene: 1501
Hk1
Name: hexokinase 1
Synonyms: Hk-1, mHk1-s, Hk1-s
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 15275
HGNC: HGNC:4922
Homologene: 100530
Lama1
Name: laminin, alpha 1
Synonyms: Lama
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 16772
VEGA: 17
HGNC: HGNC:6481
Homologene: 21146
Pfkp
Name: phosphofructokinase, platelet
Synonyms: PFK-C, 1200015H23Rik, 9330125N24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 56421
HGNC: HGNC:8878
Homologene: 20579
Dapk1
Name: death associated protein kinase 1
Synonyms: 2310039H24Rik, 2810425C21Rik, D13Ucla1, DAP-Kinase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 69635
VEGA: 13
HGNC: HGNC:2674
Homologene: 3626
Armh4
Name: armadillo-like helical domain containing 4
Synonyms: 3632451O06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 67419
Homologene: 12127
Zmiz1
Name: zinc finger, MIZ-type containing 1
Synonyms: Rai17, Zimp10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 328365
Homologene: 10667
Myo7a
Name: myosin VIIA
Synonyms: Myo7, USH1B, nmf371, polka, Hdb
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 17921
HGNC: HGNC:7606
Homologene: 219
Myh6
Name: myosin, heavy polypeptide 6, cardiac muscle, alpha
Synonyms: alpha myosin, Myhc-a, alpha cardiac MHC, cardiomyopathy, hypertrophic 1, Myhca, A830009F23Rik, alpha-MHC, alphaMHC
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 17888
HGNC: HGNC:7576
Homologene: 124414
Ppp6r2
Name: protein phosphatase 6, regulatory subunit 2
Synonyms: 1110033O10Rik, 8430411H09Rik, B230107H12Rik, Pp6r2, Saps2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 71474
VEGA: 15
Homologene: 36455
Tpo
Name: thyroid peroxidase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 22018
Homologene: 461
Gm10845
Name: predicted gene 10845
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 100038734
VEGA: 14
Gm16069
Name: predicted gene 16069
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 102636781
Ccdc83
Name: coiled-coil domain containing 83
Synonyms: 4930549K11Rik, 4930554C01Rik, 4932423M01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 75338
Homologene: 32709
Fktn
Name: fukutin
Synonyms: Fukutin, Fcmd
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 246179
HGNC: HGNC:3622
Homologene: 31402
Abhd2
Name: abhydrolase domain containing 2
Synonyms: LABH2, 2210009N18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 54608
Homologene: 23121
Or52ae7
Name: olfactory receptor family 52 subfamily AE member 7
Synonyms: GA_x6K02T2PBJ9-6191595-6192545, MOR26-3, Olfr608
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 258751
Zfp112
Name: zinc finger protein 112
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 57745
Homologene: 49338
Setbp1
Name: SET binding protein 1
Synonyms: Seb
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 240427
Homologene: 9192
Amz1
Name: archaelysin family metallopeptidase 1
Synonyms: 6530401C20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231842
Homologene: 18290
Csf1r
Name: colony stimulating factor 1 receptor
Synonyms: CSF-1R, M-CSFR, CD115, Fim-2, Fms, Csfmr, Fim2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 12978
HGNC: HGNC:2433
Homologene: 3817
Cd209c
Name: CD209c antigen
Synonyms: SIGNR2, mSIGNR2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 170776
HGNC: HGNC:1641
Homologene: 87186
Or5k15
Name: olfactory receptor family 5 subfamily J member 15
Synonyms: GA_x54KRFPKG5P-55108059-55107100, MOR184-6, Olfr178
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 258999
Homologene: 79412
Tbc1d30
Name: TBC1 domain family, member 30
Synonyms: 4930505D03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 74694
VEGA: 10
Homologene: 18930
Rpl11
Name: ribosomal protein L11
Synonyms: 2010203J19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 67025
Homologene: 37376
4930579G18Rik
Name: RIKEN cDNA 4930579G18 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 75913
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to T, chromosome 2 at 32,955,191 bp
  • C to T, chromosome 2 at 87,490,071 bp
  • T to C, chromosome 2 at 181,797,697 bp
  • C to A, chromosome 3 at 53,545,502 bp
  • G to A, chromosome 3 at 89,181,927 bp
  • A to G, chromosome 4 at 6,409,862 bp
  • T to C, chromosome 4 at 53,720,201 bp
  • G to A, chromosome 4 at 136,051,143 bp
  • T to A, chromosome 5 at 110,337,205 bp
  • T to C, chromosome 5 at 140,752,439 bp
  • G to A, chromosome 7 at 19,347,132 bp
  • C to T, chromosome 7 at 24,126,373 bp
  • T to C, chromosome 7 at 48,808,841 bp
  • A to G, chromosome 7 at 75,608,984 bp
  • A to G, chromosome 7 at 79,323,530 bp
  • A to T, chromosome 7 at 90,226,778 bp
  • G to A, chromosome 7 at 98,102,674 bp
  • T to C, chromosome 7 at 103,470,071 bp
  • T to C, chromosome 8 at 3,954,635 bp
  • C to A, chromosome 10 at 62,315,540 bp
  • T to A, chromosome 10 at 111,269,419 bp
  • A to G, chromosome 10 at 121,294,712 bp
  • T to C, chromosome 12 at 30,103,152 bp
  • A to G, chromosome 13 at 6,620,989 bp
  • A to G, chromosome 13 at 60,719,684 bp
  • T to C, chromosome 14 at 25,636,010 bp
  • T to A, chromosome 14 at 49,770,436 bp
  • C to A, chromosome 14 at 54,653,894 bp
  • T to A, chromosome 14 at 54,962,108 bp
  • G to T, chromosome 14 at 79,863,123 bp
  • C to T, chromosome 15 at 59,324,550 bp
  • C to T, chromosome 15 at 66,604,837 bp
  • T to G, chromosome 15 at 89,265,158 bp
  • A to C, chromosome 16 at 58,889,879 bp
  • A to G, chromosome 17 at 67,804,518 bp
  • G to A, chromosome 18 at 61,119,006 bp
  • T to C, chromosome 18 at 78,859,922 bp
  • A to T, chromosome 19 at 10,539,637 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4374 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041118-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.