Strain Name:
C57BL/6J-MtgxR4425Btlr/Mmmh
Stock Number:
041144-MU
Citation ID:
RRID:MMRRC_041144-MU
Other Names:
R4425 (G1), C57BL/6J-MtgxR4425Btlr
Major Collection:

Gene Information

Ppp1r16b
Name: protein phosphatase 1, regulatory subunit 16B
Synonyms: C130078N17Rik, Wdt4, ANKRD4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 228852
Homologene: 9194
Zfp423
Name: zinc finger protein 423
Synonyms: ataxia1, Roaz, Ebfaz, Zfp104
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 94187
Homologene: 9010
Lrig3
Name: leucine-rich repeats and immunoglobulin-like domains 3
Synonyms: 9430095K15Rik, 9030421L11Rik, 9130004I02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 320398
VEGA: 10
Homologene: 62452
Epb41l2
Name: erythrocyte membrane protein band 4.1 like 2
Synonyms: D10Ertd398e, 4.1G, Epb4.1l2, NBL2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 13822
VEGA: 10
HGNC: HGNC:3379
Homologene: 37478
Top2a
Name: topoisomerase (DNA) II alpha
Synonyms: Top-2, DNA Topoisomerase II alpha
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 21973
Homologene: 830
Nfrkb
Name: nuclear factor related to kappa B binding protein
Synonyms: A530090G11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 235134
HGNC: HGNC:7802
Homologene: 4492
Rims2
Name: regulating synaptic membrane exocytosis 2
Synonyms: 2810036I15Rik, RIM2, Syt3-rs
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 116838
VEGA: 15
Homologene: 81639
Arfgap1
Name: ADP-ribosylation factor GTPase activating protein 1
Synonyms: ARF1 GAP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 228998
Homologene: 5517
Rad51
Name: RAD51 recombinase
Synonyms: Reca, Rad51a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 19361
HGNC: HGNC:9817
Homologene: 2155
Eef1d
Name: eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein)
Synonyms: 5730529A16Rik, 1700026P12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 66656
HGNC: HGNC:3211
Homologene: 23404
Syvn1
Name: synovial apoptosis inhibitor 1, synoviolin
Synonyms: 1200010C09Rik, Hrd1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 74126
Homologene: 32700
Efl1
Name: elongation factor like GTPase 1
Synonyms: Eftud1, 4932434J20Rik, 6030468D11Rik, D7Ertd791e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 101592
Homologene: 11599
Lrrc8c
Name: leucine rich repeat containing 8 family, member C
Synonyms: E430036I04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 100604
Homologene: 12997
Cngb1
Name: cyclic nucleotide gated channel beta 1
Synonyms: Cngb1, Cngb1b, BC016201
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 333329
HGNC: HGNC:2151
Homologene: 136420
Tek
Name: TEK receptor tyrosine kinase
Synonyms: tie-2, Tie2, Cd202b, Hyk
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 21687
Homologene: 397
Ttn
Name: titin
Synonyms: shru, connectin, mdm, 2310057K23Rik, D330041I19Rik, 1100001C23Rik, D830007G01Rik, 2310036G12Rik, L56, 2310074I15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 22138
Homologene: 130650
Capns2
Name: calpain, small subunit 2
Synonyms: 30K-2, 2310005G05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 69543
Homologene: 57167
Arap3
Name: ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 3
Synonyms: E030006K04Rik, Centd3, DRAG1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 106952
VEGA: 18
Homologene: 11199
Arhgap30
Name: Rho GTPase activating protein 30
Synonyms: 6030405P05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 226652
Homologene: 18766
Slc35f4
Name: solute carrier family 35, member F4
Synonyms: 4930550L21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 75288
Homologene: 45939
Fhod1
Name: formin homology 2 domain containing 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 234686
Homologene: 136406
Tpo
Name: thyroid peroxidase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 22018
Homologene: 461
Fbxl4
Name: F-box and leucine-rich repeat protein 4
Synonyms: FBL4, FBL5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 269514
Homologene: 8128
Ceacam10
Name: carcinoembryonic antigen-related cell adhesion molecule 10
Synonyms: Bgp3, Cea10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 26366
Vsig8
Name: V-set and immunoglobulin domain containing 8
Synonyms: EG240916
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 240916
Homologene: 18616
Vwa8
Name: von Willebrand factor A domain containing 8
Synonyms: 4932416F07Rik, 1300010F03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 219189
VEGA: 14
Homologene: 44881
Dalrd3
Name: DALR anticodon binding domain containing 3
Synonyms: 6330580J24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 67789
Homologene: 32388
Shkbp1
Name: Sh3kbp1 binding protein 1
Synonyms: SB1, B930062H15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 192192
Homologene: 26700
Krt78
Name: keratin 78
Synonyms: 2310030B04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 332131
VEGA: 15
Homologene: 65261
Acad11
Name: acyl-Coenzyme A dehydrogenase family, member 11
Synonyms: 5730439E10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 102632
Homologene: 49896
Vmn1r228
Name: vomeronasal 1 receptor 228
Synonyms: V1re3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 171226
Homologene: 74320
Zfp811
Name: zinc finger protein 811
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 240063
Cyp2c50
Name: cytochrome P450, family 2, subfamily c, polypeptide 50
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 107141
Homologene: 137231
Abcc3
Name: ATP-binding cassette, sub-family C (CFTR/MRP), member 3
Synonyms: MRP3, 1700019L09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 76408
HGNC: HGNC:54
Homologene: 68364
Cfh
Name: complement component factor h
Synonyms: Sas1, Sas-1, Mud-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 12628
HGNC: HGNC:4883
Homologene: 20086
Gm12500
Name: predicted gene 12500
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 791415
Cep44
Name: centrosomal protein 44
Synonyms: 4933440G23Rik, BC088983
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 382010
Homologene: 12738
Elmo1
Name: engulfment and cell motility 1
Synonyms: 6330578D22Rik, C230095H21Rik, CED-12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 140580
Homologene: 56685
Chrnb1
Name: cholinergic receptor, nicotinic, beta polypeptide 1 (muscle)
Synonyms: Acrb, Achr-2, AChR beta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 11443
HGNC: HGNC:1961
Homologene: 594
Il10rb
Name: interleukin 10 receptor, beta
Synonyms: D21S58h, Il10r2, D16H21S58, Crfb4, CRF2-4, 6620401D04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 16155
HGNC: HGNC:5965
Homologene: 523
Sh3rf3
Name: SH3 domain containing ring finger 3
Synonyms: Sh3md4, 4831416G18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 237353
Homologene: 77551
Vmn1r183
Name: vomeronasal 1 receptor 183
Synonyms: LOC209824, V1rd15
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 209824
Homologene: 104166
Cd163
Name: CD163 antigen
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 93671
HGNC: HGNC:1631
Homologene: 128811
Olfr713
Name: olfactory receptor 713
Synonyms: P3, GA_x6K02T2PBJ9-9415724-9416677, MOR263-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 259036
Homologene: 17470
Amh
Name: anti-Mullerian hormone
Synonyms: MIS, Mullerian inhibiting substance
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 11705
VEGA: 10
HGNC: HGNC:464
Homologene: 68060
Adamts8
Name: a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 8
Synonyms: METH2, METH-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 30806
HGNC: HGNC:224
Homologene: 5108
Olfr1307
Name: olfactory receptor 1307
Synonyms: GA_x6K02T2Q125-72988111-72987173, MOR245-19P
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 257956
Homologene: 128383
Nphp1
Name: nephronophthisis 1 (juvenile) homolog (human)
Synonyms: nephrocystin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 53885
HGNC: HGNC:7905
Homologene: 229
Tagap1
Name: T cell activation GTPase activating protein 1
Synonyms: 2610315E15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 380608
VEGA: 17
Tbcb
Name: tubulin folding cofactor B
Synonyms: 2410007D12Rik, Ckap1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 66411
HGNC: HGNC:1989
Homologene: 981
Igkv10-95
Name: immunoglobulin kappa variable 10-95
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 434031
Gm21761
Name: predicted gene, 21761
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 106029237
Trav12-2
Name: T cell receptor alpha variable 12-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 100043322
Gm8741
Name: predicted gene 8741
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 667636
Pcdhgc4
Name: protocadherin gamma subfamily C, 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 93707
HGNC: HGNC:8717
Homologene: 110932
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 140,100,875 bp
  • C to T, chromosome 1 at 171,407,383 bp
  • G to T, chromosome 1 at 172,563,147 bp
  • G to A, chromosome 2 at 76,903,086 bp
  • T to C, chromosome 2 at 111,945,189 bp
  • T to A, chromosome 2 at 119,134,194 bp
  • T to C, chromosome 2 at 127,788,799 bp
  • C to T, chromosome 2 at 158,757,254 bp
  • C to A, chromosome 2 at 180,981,076 bp
  • T to C, chromosome 3 at 108,086,736 bp
  • T to A, chromosome 4 at 22,422,699 bp
  • A to G, chromosome 4 at 94,863,667 bp
  • T to A, chromosome 5 at 105,607,889 bp
  • A to T, chromosome 6 at 68,680,622 bp
  • T to C, chromosome 6 at 124,327,903 bp
  • A to T, chromosome 7 at 24,055,548 bp
  • A to T, chromosome 7 at 24,781,008 bp
  • T to C, chromosome 7 at 27,343,302 bp
  • A to T, chromosome 7 at 30,235,364 bp
  • G to A, chromosome 7 at 82,763,283 bp
  • A to C, chromosome 7 at 107,036,491 bp
  • G to A, chromosome 8 at 56,538,617 bp
  • G to A, chromosome 8 at 87,782,973 bp
  • T to A, chromosome 8 at 92,901,624 bp
  • C to T, chromosome 8 at 95,299,716 bp
  • T to C, chromosome 8 at 105,337,351 bp
  • A to G, chromosome 9 at 30,956,656 bp
  • G to T, chromosome 9 at 31,399,962 bp
  • T to C, chromosome 9 at 104,073,645 bp
  • T to C, chromosome 9 at 108,571,601 bp
  • C to A, chromosome 10 at 25,506,180 bp
  • T to C, chromosome 10 at 59,083,576 bp
  • A to G, chromosome 10 at 80,806,921 bp
  • T to C, chromosome 10 at 126,013,404 bp
  • A to T, chromosome 11 at 69,786,947 bp
  • T to C, chromosome 11 at 94,346,044 bp
  • A to G, chromosome 11 at 99,001,405 bp
  • T to C, chromosome 12 at 30,104,016 bp
  • C to G, chromosome 13 at 20,600,212 bp
  • C to A, chromosome 13 at 119,912,372 bp
  • C to T, chromosome 14 at 49,318,850 bp
  • A to G, chromosome 14 at 53,616,875 bp
  • A to G, chromosome 14 at 79,082,806 bp
  • T to C, chromosome 15 at 39,437,924 bp
  • C to A, chromosome 15 at 75,902,799 bp
  • T to C, chromosome 15 at 101,947,940 bp
  • C to A, chromosome 16 at 91,407,715 bp
  • G to A, chromosome 17 at 6,956,112 bp
  • T to C, chromosome 17 at 20,776,599 bp
  • A to T, chromosome 17 at 32,797,547 bp
  • G to T, chromosome 17 at 35,336,086 bp
  • A to G, chromosome 18 at 37,815,988 bp
  • A to G, chromosome 18 at 37,978,600 bp
  • C to T, chromosome 19 at 6,049,921 bp
  • A to T, chromosome 19 at 40,090,692 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4425 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041144-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.