Strain Name:
C57BL/6J-MtgxR4427Btlr/Mmmh
Stock Number:
041145-MU
Citation ID:
RRID:MMRRC_041145-MU
Other Names:
R4427 (G1), C57BL/6J-MtgxR4427Btlr
Major Collection:

Gene Information

Frem2
Name: Fras1 related extracellular matrix protein 2
Synonyms: b2b1562Clo, 8430406N05Rik, my, ne, 6030440P17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 242022
Homologene: 18454
Dst
Name: dystonin
Synonyms: bullous pemphigoid antigen 1, BPAG1, Bpag, Macf2, Bpag1, nmf339, BPAG1-n, ah, bullous pemphigoid antigen 1, athetoid, A830042E19Rik, 2310001O04Rik, nmf203
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 13518
HGNC: HGNC:1090
Homologene: 136716
Crybg3
Name: beta-gamma crystallin domain containing 3
Synonyms: Gm9581
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 224273
Homologene: 28544
Rps6ka2
Name: ribosomal protein S6 kinase, polypeptide 2
Synonyms: Rsk3, Rps6ka-rs1, 90kDa, p90rsk, pp90rsk, D17Wsu134e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 20112
VEGA: 17
Homologene: 100680
Pi4ka
Name: phosphatidylinositol 4-kinase alpha
Synonyms: Pik4ca
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 224020
HGNC: HGNC:8983
Homologene: 11171
Ogdh
Name: oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide)
Synonyms: 2210403E04Rik, alpha-ketoglutarate dehydrogenase, 2210412K19Rik, d1401
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 18293
HGNC: HGNC:8124
Homologene: 55662
Utp18
Name: UTP18 small subunit processome component
Synonyms: 6230425C22Rik, Wdr50
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 217109
Homologene: 41087
Herc1
Name: HECT and RLD domain containing E3 ubiquitin protein ligase family member 1
Synonyms: 2810449H11Rik, D130015N03Rik, tbl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235439
HGNC: HGNC:4867
Homologene: 31207
Lamc1
Name: laminin, gamma 1
Synonyms: laminin B2, Lamb2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 226519
HGNC: HGNC:6492
Homologene: 1724
Zfp982
Name: zinc finger protein 982
Synonyms: Gm13152
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 195531
Gas2l1
Name: growth arrest-specific 2 like 1
Synonyms: 4930500E24Rik, TU-71.1, GAR22, D0Jmb1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 78926
Homologene: 4733
Vwc2
Name: von Willebrand factor C domain containing 2
Synonyms: A930041G11Rik, Brorin, cradin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 319922
Homologene: 18542
Tpm1
Name: tropomyosin 1, alpha
Synonyms: Tm3, alpha-TM, TM2, Tpm-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 22003
Homologene: 128637
Pwwp2a
Name: PWWP domain containing 2A
Synonyms: D930040F23Rik, 4631424J17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 70802
Homologene: 19687
Memo1
Name: mediator of cell motility 1
Synonyms: 0610016J10Rik, D930048L02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 76890
VEGA: 17
Homologene: 6272
Phactr4
Name: phosphatase and actin regulator 4
Synonyms: 3110001B12Rik, C330013F19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 100169
Homologene: 41537
Exoc1
Name: exocyst complex component 1
Synonyms: Sec3p, A730011E05Rik, SEC3, Sec3l1, 2810407P21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 69940
Homologene: 41241
BC055324
Name: cDNA sequence BC055324
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 381306
Homologene: 10058
Siglec15
Name: sialic acid binding Ig-like lectin 15
Synonyms: Cd33l3, EG620235
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 620235
Homologene: 52643
Klhl8
Name: kelch-like 8
Synonyms: 2310001P09Rik, D5Ertd431e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 246293
Homologene: 10819
Trmt2a
Name: TRM2 tRNA methyltransferase 2A
Synonyms: Htf9c
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 15547
Homologene: 7374
Tcaim
Name: T cell activation inhibitor, mitochondrial
Synonyms: LOC382117, D9Ertd402e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 382117
Homologene: 45481
Kcnd2
Name: potassium voltage-gated channel, Shal-related family, member 2
Synonyms: Kv4.2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 16508
HGNC: HGNC:6238
Homologene: 40828
Rap1gap2
Name: RAP1 GTPase activating protein 2
Synonyms: LOC380710, Garnl4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 380711
Homologene: 56695
4930432E11Rik
Name: RIKEN cDNA 4930432E11 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 243900
Rcsd1
Name: RCSD domain containing 1
Synonyms: A430105K13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 226594
Homologene: 33475
Ugcg
Name: UDP-glucose ceramide glucosyltransferase
Synonyms: Ugcgl, GlcT-1, Epcs21
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 22234
Homologene: 37763
Cryga
Name: crystallin, gamma A
Synonyms: Secc, Cryg-4, DGcry-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 12964
HGNC: HGNC:2408
Homologene: 129704
Ltf
Name: lactotransferrin
Synonyms: Lf, lactoferrin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 17002
HGNC: HGNC:6720
Homologene: 1754
A2ml1
Name: alpha-2-macroglobulin like 1
Synonyms: Ovos2, BC048546
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 232400
Homologene: 45969
Ppp1r9b
Name: protein phosphatase 1, regulatory subunit 9B
Synonyms: SPL, spinophilin, neurabin II, Spn
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 217124
HGNC: HGNC:9298
Homologene: 32787
Vmn2r73
Name: vomeronasal 2, receptor 73
Synonyms: EG620928
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 620928
Homologene: 115466
Thbs2
Name: thrombospondin 2
Synonyms: Thrombospondin-2, Thbs-2, TSP2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 21826
VEGA: 17
Homologene: 2438
Ccm2l
Name: cerebral cavernous malformation 2-like
Synonyms: BC020535
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 228788
Homologene: 130991
Ccdc189
Name: coiled-coil domain containing 189
Synonyms: Gm166, LOC233899
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233899
Homologene: 79950
Tmx3
Name: thioredoxin-related transmembrane protein 3
Synonyms: A730024F05Rik, Txndc10, 6430411B10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 67988
VEGA: 18
Homologene: 10381
Krt7
Name: keratin 7
Synonyms: Cytokeratin 7, D15Wsu77e, K7, Krt2-7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 110310
HGNC: HGNC:6445
Homologene: 4058
Sgce
Name: sarcoglycan, epsilon
Synonyms: e-SG
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 20392
Homologene: 31205
Klhl30
Name: kelch-like 30
Synonyms: 4631423F02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 70788
Homologene: 18891
Poc1b
Name: POC1 centriolar protein B
Synonyms: Wdr51b, 4933430F16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 382406
Homologene: 41728
Zfp300
Name: zinc finger protein 300
Synonyms: D930016N04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 245368
Homologene: 65054
Gsto2
Name: glutathione S-transferase omega 2
Synonyms: 1700020F09Rik, 4930425C18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 68214
Homologene: 57057
Evi2b
Name: ecotropic viral integration site 2b
Synonyms: Evi2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 216984
HGNC: HGNC:3500
Homologene: 48438
Rab36
Name: RAB36, member RAS oncogene family
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 76877
HGNC: HGNC:9775
Homologene: 3610
BC053393
Name: cDNA sequence BC053393
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 407814
Homologene: 86792
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 34,181,460 bp
  • A to T, chromosome 1 at 65,100,616 bp
  • A to G, chromosome 1 at 91,353,704 bp
  • T to C, chromosome 1 at 153,249,633 bp
  • A to G, chromosome 1 at 163,954,284 bp
  • C to A, chromosome 1 at 165,655,895 bp
  • A to G, chromosome 2 at 153,066,027 bp
  • A to G, chromosome 3 at 53,539,162 bp
  • T to A, chromosome 4 at 59,219,555 bp
  • T to C, chromosome 4 at 132,387,041 bp
  • A to T, chromosome 4 at 147,512,623 bp
  • A to G, chromosome 5 at 76,563,263 bp
  • G to A, chromosome 5 at 103,874,294 bp
  • G to A, chromosome 6 at 4,691,459 bp
  • T to A, chromosome 6 at 21,216,897 bp
  • T to C, chromosome 6 at 128,545,046 bp
  • A to T, chromosome 7 at 29,579,253 bp
  • A to G, chromosome 7 at 85,857,773 bp
  • A to G, chromosome 7 at 127,588,116 bp
  • T to C, chromosome 9 at 66,496,005 bp
  • A to G, chromosome 9 at 67,032,565 bp
  • C to A, chromosome 9 at 111,023,604 bp
  • T to C, chromosome 9 at 122,814,496 bp
  • G to A, chromosome 10 at 75,044,496 bp
  • T to C, chromosome 10 at 99,155,139 bp
  • A to G, chromosome 11 at 5,063,908 bp
  • C to T, chromosome 11 at 6,355,421 bp
  • A to G, chromosome 11 at 11,154,235 bp
  • T to C, chromosome 11 at 43,682,517 bp
  • T to A, chromosome 11 at 46,584,420 bp
  • C to A, chromosome 11 at 74,407,322 bp
  • T to A, chromosome 11 at 79,516,356 bp
  • T to C, chromosome 11 at 93,866,438 bp
  • A to T, chromosome 11 at 95,001,324 bp
  • T to A, chromosome 15 at 101,420,497 bp
  • C to T, chromosome 16 at 17,281,044 bp
  • A to G, chromosome 16 at 18,249,229 bp
  • T to C, chromosome 16 at 59,543,199 bp
  • T to A, chromosome 17 at 7,299,405 bp
  • C to A, chromosome 17 at 14,680,335 bp
  • T to A, chromosome 17 at 74,202,307 bp
  • T to G, chromosome 18 at 78,043,621 bp
  • T to A, chromosome 18 at 90,523,601 bp
  • T to C, chromosome 19 at 47,871,773 bp
  • C to T, chromosome X at 21,083,166 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4427 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041145-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.