Strain Name:
C57BL/6J-MtgxR4431Btlr/Mmmh
Stock Number:
041146-MU
Citation ID:
RRID:MMRRC_041146-MU
Other Names:
R4431 (G1), C57BL/6J-MtgxR4431Btlr
Major Collection:

Gene Information

Adamts20
Name: a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 20
Synonyms: ADAMTS-20, bt
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 223838
Homologene: 11808
Chrna4
Name: cholinergic receptor, nicotinic, alpha polypeptide 4
Synonyms: Acra-4, Acra4, a4 nicotinic receptor, alpha4 nAChR, alpha4-nAChR
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 11438
HGNC: HGNC:1958
Homologene: 592
Tert
Name: telomerase reverse transcriptase
Synonyms: TR
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 21752
VEGA: 13
Homologene: 31141
Strn
Name: striatin, calmodulin binding protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 268980
Homologene: 2380
Ttc3
Name: tetratricopeptide repeat domain 3
Synonyms: TPRD, D16Ium21e, 2610202A04Rik, D16Ium21
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 22129
Homologene: 2487
Pomp
Name: proteasome maturation protein
Synonyms: 2510048O06Rik, Ump1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 66537
Homologene: 41077
Fbxo42
Name: F-box protein 42
Synonyms: 6720460I06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 213499
Homologene: 16234
Uhrf1bp1
Name: UHRF1 (ICBP90) binding protein 1
Synonyms: F830021D11Rik, 1110020K19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 224648
Homologene: 9816
Slc12a3
Name: solute carrier family 12, member 3
Synonyms: TSC, NCC
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 20497
Homologene: 287
Ints12
Name: integrator complex subunit 12
Synonyms: 1110020M19Rik, A230056J18Rik, 4930529N21Rik, Phf22, 2810027J24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 71793
Homologene: 32474
Trf
Name: transferrin
Synonyms: Tfn, HP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 22041
Homologene: 68153
Wnt2b
Name: wingless-type MMTV integration site family, member 2B
Synonyms: Wnt13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 22414
Homologene: 22526
Chd2
Name: chromodomain helicase DNA binding protein 2
Synonyms: 2810040A01Rik, 5630401D06Rik, 2810013C04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 244059
HGNC: HGNC:1917
Homologene: 37462
Lamc1
Name: laminin, gamma 1
Synonyms: laminin B2, Lamb2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 226519
HGNC: HGNC:6492
Homologene: 1724
Vps13b
Name: vacuolar protein sorting 13B
Synonyms: 2310042E16Rik, Coh1, 1810042B05Rik, C330002D13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 666173
VEGA: 15
HGNC: HGNC:2183
Homologene: 49516
Wdfy1
Name: WD repeat and FYVE domain containing 1
Synonyms: 1700120F24Rik, 1700013B03Rik, Jr1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 69368
Homologene: 10825
Lhfpl4
Name: lipoma HMGIC fusion partner-like protein 4
Synonyms: 1190004M23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 269788
Homologene: 14943
Gpr68
Name: G protein-coupled receptor 68
Synonyms: OGR1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 238377
HGNC: HGNC:4519
Homologene: 2603
4930522L14Rik
Name: RIKEN cDNA 4930522L14 gene
Synonyms: Gm42152
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 100041734
HGNC: null
Homologene: null
Cldn8
Name: claudin 8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 54420
HGNC: HGNC:2050
Homologene: 8117
Gtpbp1
Name: GTP binding protein 1
Synonyms: GTPBP1, GP-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 14904
VEGA: 15
HGNC: HGNC:4669
Homologene: 3165
Aak1
Name: AP2 associated kinase 1
Synonyms: D6Ertd245e, 5530400K14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 269774
Homologene: 128746
Slc12a9
Name: solute carrier family 12 (potassium/chloride transporters), member 9
Synonyms: CIP1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 83704
Homologene: 5429
Pkhd1
Name: polycystic kidney and hepatic disease 1
Synonyms: FPC, tigmin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 241035
HGNC: HGNC:9016
Homologene: 16336
Mipol1
Name: mirror-image polydactyly 1
Synonyms: D12Ertd19e, 1700081O04Rik, 6030439O22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 73490
Homologene: 16340
Pus7
Name: pseudouridylate synthase 7
Synonyms: C330017I15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 78697
Homologene: 6998
Bspry
Name: B-box and SPRY domain containing
Synonyms: zetin 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 192120
Homologene: 9781
Gramd4
Name: GRAM domain containing 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 223752
Homologene: 18199
Cfap300
Name: cilia and flagella associated protein 300
Synonyms: 9230110C19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 234912
VEGA: 9
Homologene: 13163
Shank1
Name: SH3 and multiple ankyrin repeat domains 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 243961
Homologene: 22949
Chrm2
Name: cholinergic receptor, muscarinic 2, cardiac
Synonyms: Chrm-2, muscarinic acetylcholine receptor 2, M2, AChR M2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 243764
HGNC: HGNC:1951
Homologene: 20190
Nuggc
Name: nuclear GTPase, germinal center associated
Synonyms: SLIP-GC, LOC239151, Gm600
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 100503545
Homologene: 72641
Olfr1243
Name: olfactory receptor 1243
Synonyms: MOR231-4, GA_x6K02T2Q125-50972538-50971621
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 258971
HGNC: null
Homologene: 27317
Abca2
Name: ATP-binding cassette, sub-family A (ABC1), member 2
Synonyms: D2H0S1474E, Abc2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 11305
HGNC: HGNC:32
Homologene: 55590
Ptar1
Name: protein prenyltransferase alpha subunit repeat containing 1
Synonyms: 2010013L17Rik, 4930428J16Rik, 1700084D09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 72351
VEGA: 19
Homologene: 34254
Klhl25
Name: kelch-like 25
Synonyms: 2810402K13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 207952
Homologene: 32546
Man1c1
Name: mannosidase, alpha, class 1C, member 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 230815
Homologene: 69303
0610040J01Rik
Name: RIKEN cDNA 0610040J01 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 76261
Homologene: 49537
Cfh
Name: complement component factor h
Synonyms: Sas1, Sas-1, Mud-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 12628
HGNC: HGNC:4883
Homologene: 20086
Vmn2r22
Name: vomeronasal 2, receptor 22
Synonyms: EG546913
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 546913
HGNC: null
Homologene: 84037
Atxn7l1
Name: ataxin 7-like 1
Synonyms: Atxn7l4, 2810423G08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 380753
Homologene: 53366
Fmo1
Name: flavin containing monooxygenase 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 14261
HGNC: HGNC:3769
Homologene: 55520
Tmem67
Name: transmembrane protein 67
Synonyms: 5330408M12Rik, b2b1163.1Clo, b2b1291.1Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 329795
Homologene: 71886
Lrrc71
Name: leucine rich repeat containing 71
Synonyms: 4933430H15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 74485
Homologene: 83646
Bag4
Name: BCL2-associated athanogene 4
Synonyms: 2410112I15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 67384
HGNC: HGNC:940
Homologene: 31270
Sytl5
Name: synaptotagmin-like 5
Synonyms: ENSMUSG00000054453
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
Alteration at locus: Chemically Induced
NCBI: 236643
Homologene: 16345
2510039O18Rik
Name: RIKEN cDNA 2510039O18 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 77034
Homologene: 12668
Spz1
Name: spermatogenic leucine zipper 1
Synonyms: 1700027M20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 79401
VEGA: 13
Homologene: 49934
4930523C07Rik
Name: RIKEN cDNA 4930523C07 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 67647
Homologene: 124386
Kat2b-ps
Name: K(lysine) acetyltransferase 2B, pseudogene
Synonyms: P/CAF pseudogene, Pcaf-ps
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 100504388
HGNC: null
Homologene: null
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 20,523,314 bp
  • T to A, chromosome 1 at 79,713,866 bp
  • T to C, chromosome 1 at 140,136,266 bp
  • A to G, chromosome 1 at 153,221,528 bp
  • A to T, chromosome 1 at 160,044,579 bp
  • G to A, chromosome 1 at 162,833,712 bp
  • A to G, chromosome 2 at 25,442,852 bp
  • A to G, chromosome 2 at 89,527,643 bp
  • A to G, chromosome 2 at 181,028,620 bp
  • A to G, chromosome 3 at 87,742,836 bp
  • A to G, chromosome 3 at 104,952,940 bp
  • T to C, chromosome 3 at 133,102,481 bp
  • T to A, chromosome 4 at 12,051,473 bp
  • T to A, chromosome 4 at 62,482,667 bp
  • A to T, chromosome 4 at 134,703,018 bp
  • C to A, chromosome 4 at 141,200,550 bp
  • T to C, chromosome 4 at 147,941,565 bp
  • A to G, chromosome 5 at 23,746,489 bp
  • G to T, chromosome 5 at 63,898,839 bp
  • A to T, chromosome 5 at 93,392,584 bp
  • A to T, chromosome 5 at 109,736,574 bp
  • G to A, chromosome 5 at 137,321,513 bp
  • A to G, chromosome 5 at 147,875,479 bp
  • A to G, chromosome 6 at 36,524,162 bp
  • A to G, chromosome 6 at 86,986,318 bp
  • A to G, chromosome 6 at 113,193,844 bp
  • T to C, chromosome 6 at 123,637,858 bp
  • C to T, chromosome 7 at 44,319,652 bp
  • T to C, chromosome 7 at 73,435,961 bp
  • T to A, chromosome 7 at 75,865,414 bp
  • C to T, chromosome 8 at 25,769,488 bp
  • A to T, chromosome 8 at 94,343,085 bp
  • A to G, chromosome 9 at 8,027,177 bp
  • T to C, chromosome 9 at 103,211,876 bp
  • C to T, chromosome 12 at 33,326,065 bp
  • G to A, chromosome 12 at 57,303,524 bp
  • A to T, chromosome 12 at 100,899,391 bp
  • T to A, chromosome 13 at 73,627,475 bp
  • A to G, chromosome 13 at 92,575,329 bp
  • T to A, chromosome 14 at 65,611,210 bp
  • A to C, chromosome 15 at 35,770,753 bp
  • T to G, chromosome 15 at 79,716,197 bp
  • A to G, chromosome 15 at 86,130,160 bp
  • C to A, chromosome 15 at 94,344,043 bp
  • A to C, chromosome 16 at 88,562,731 bp
  • T to C, chromosome 16 at 94,410,958 bp
  • A to G, chromosome 17 at 27,885,931 bp
  • A to G, chromosome 17 at 78,736,462 bp
  • G to T, chromosome 19 at 23,694,331 bp
  • A to T, chromosome X at 9,960,023 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4431 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041146-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.