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Strain Name:
C57BL/6J-MtgxR4445Btlr/Mmmh
Stock Number:
041151-MU
Citation ID:
RRID:MMRRC_041151-MU
Other Names:
R4445 (G1), C57BL/6J-MtgxR4445Btlr
Major Collection:

Strain Information

Cntnap2
Name: contactin associated protein-like 2
Synonyms: 5430425M22Rik, Caspr2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 66797
Homologene: 69159
Mtmr3
Name: myotubularin related protein 3
Synonyms: FYVE-DSP1, 1700092A20Rik, ZFYVE10
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 74302
HGNC: HGNC:7451
Homologene: 23662
Lyst
Name: lysosomal trafficking regulator
Synonyms: D13Sfk13
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 17101
VEGA: 13
HGNC: HGNC:1968
Homologene: 61
Cd59a
Name: CD59a antigen
Synonyms: Cd59, protectin
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 12509
HGNC: HGNC:1689
Epb41l2
Name: erythrocyte membrane protein band 4.1 like 2
Synonyms: NBL2, 4.1G, D10Ertd398e, Epb4.1l2
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 13822
VEGA: 10
HGNC: HGNC:3379
Homologene: 37478
Tbce
Name: tubulin-specific chaperone E
Synonyms: 2610206D02Rik, C530005D02Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 70430
Homologene: 37744
Ppil2
Name: peptidylprolyl isomerase (cyclophilin)-like 2
Synonyms: C130078A06Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 66053
HGNC: HGNC:9261
Homologene: 8643
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 58,666,921 bp
  • G to A, chromosome 1 at 172,535,227 bp
  • T to G, chromosome 2 at 36,937,551 bp
  • A to G, chromosome 2 at 76,784,833 bp
  • A to G, chromosome 2 at 76,856,866 bp
  • A to G, chromosome 2 at 104,110,818 bp
  • G to A, chromosome 3 at 109,098,668 bp
  • A to T, chromosome 3 at 129,788,531 bp
  • A to G, chromosome 4 at 33,499,035 bp
  • G to A, chromosome 4 at 115,326,783 bp
  • T to C, chromosome 5 at 8,973,643 bp
  • G to A, chromosome 5 at 92,020,309 bp
  • T to C, chromosome 5 at 121,525,228 bp
  • G to T, chromosome 5 at 123,148,104 bp
  • C to T, chromosome 6 at 46,759,851 bp
  • T to C, chromosome 6 at 50,043,630 bp
  • T to G, chromosome 6 at 57,137,530 bp
  • C to T, chromosome 7 at 42,042,450 bp
  • T to C, chromosome 7 at 98,066,404 bp
  • T to C, chromosome 7 at 102,948,798 bp
  • T to A, chromosome 7 at 106,689,146 bp
  • C to A, chromosome 7 at 140,574,389 bp
  • C to T, chromosome 8 at 45,277,055 bp
  • G to A, chromosome 8 at 70,290,143 bp
  • T to C, chromosome 8 at 83,934,860 bp
  • A to G, chromosome 9 at 27,334,252 bp
  • T to A, chromosome 9 at 53,934,129 bp
  • T to G, chromosome 9 at 67,982,495 bp
  • C to T, chromosome 9 at 71,869,063 bp
  • A to G, chromosome 10 at 14,409,763 bp
  • T to C, chromosome 10 at 25,443,803 bp
  • A to C, chromosome 10 at 79,379,040 bp
  • G to A, chromosome 11 at 4,488,143 bp
  • A to T, chromosome 11 at 6,265,770 bp
  • G to A, chromosome 11 at 77,471,441 bp
  • C to G, chromosome 11 at 94,097,253 bp
  • G to A, chromosome 12 at 116,207,715 bp
  • T to C, chromosome 13 at 13,709,564 bp
  • A to T, chromosome 13 at 13,998,395 bp
  • T to C, chromosome 13 at 21,750,343 bp
  • G to A, chromosome 13 at 69,629,721 bp
  • T to C, chromosome 13 at 80,889,063 bp
  • A to T, chromosome 14 at 52,204,527 bp
  • A to G, chromosome 14 at 61,204,686 bp
  • G to A, chromosome 14 at 73,029,893 bp
  • A to G, chromosome 15 at 8,252,188 bp
  • A to G, chromosome 15 at 91,350,020 bp
  • A to G, chromosome 15 at 102,715,740 bp
  • A to T, chromosome 16 at 17,103,600 bp
  • A to G, chromosome 16 at 58,461,007 bp
  • A to T, chromosome 16 at 59,624,313 bp
  • A to G, chromosome 17 at 27,145,102 bp
  • T to C, chromosome 18 at 39,774,200 bp
  • A to T, chromosome 19 at 5,502,994 bp
  • A to T, chromosome 19 at 41,963,933 bp
  • A to G, chromosome 19 at 46,668,023 bp
  • T to C, chromosome 19 at 55,302,737 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4445 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041151-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.