Strain Name:
C57BL/6J-MtgxR4445Btlr/Mmmh
Stock Number:
041151-MU
Citation ID:
RRID:MMRRC_041151-MU
Other Names:
R4445 (G1), C57BL/6J-MtgxR4445Btlr
Major Collection:

Gene Information

Cntnap2
Name: contactin associated protein-like 2
Synonyms: 5430425M22Rik, Caspr2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 66797
Homologene: 69159
Mtmr3
Name: myotubularin related protein 3
Synonyms: FYVE-DSP1, 1700092A20Rik, ZFYVE10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 74302
HGNC: HGNC:7451
Homologene: 23662
Lyst
Name: lysosomal trafficking regulator
Synonyms: D13Sfk13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 17101
VEGA: 13
HGNC: HGNC:1968
Homologene: 61
Cd59a
Name: CD59a antigen
Synonyms: Cd59, protectin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 12509
HGNC: HGNC:1689
Epb41l2
Name: erythrocyte membrane protein band 4.1 like 2
Synonyms: NBL2, 4.1G, D10Ertd398e, Epb4.1l2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 13822
VEGA: 10
HGNC: HGNC:3379
Homologene: 37478
Tbce
Name: tubulin-specific chaperone E
Synonyms: 2610206D02Rik, C530005D02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 70430
Homologene: 37744
Ppil2
Name: peptidylprolyl isomerase (cyclophilin)-like 2
Synonyms: C130078A06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 66053
HGNC: HGNC:9261
Homologene: 8643
Rngtt
Name: RNA guanylyltransferase and 5'-phosphatase
Synonyms: mouse capping enzyme
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 24018
Homologene: 37851
Mapkapk5
Name: MAP kinase-activated protein kinase 5
Synonyms: PRAK, MK5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 17165
HGNC: HGNC:6889
Homologene: 69077
Vps13c
Name: vacuolar protein sorting 13C
Synonyms: C230055H22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 320528
VEGA: 9
Homologene: 41188
Ddx56
Name: DEAD box helicase 56
Synonyms: 2600001H07Rik, NOH61, D11Ertd619e, DEAD (Asp-Glu-Ala-Asp) box polypeptide 56
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 52513
Homologene: 6498
Tcf12
Name: transcription factor 12
Synonyms: ME1, ALF1, HEB, HTF4, HTF-4, REB, bHLHb20, HEBAlt
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 21406
Homologene: 40774
Mms19
Name: MMS19 cytosolic iron-sulfur assembly component
Synonyms: 2610042O15Rik, Mms19, C86341, Mms19l
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 72199
Homologene: 41480
Arrdc3
Name: arrestin domain containing 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 105171
Homologene: 69318
Crot
Name: carnitine O-octanoyltransferase
Synonyms: 1200003H03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 74114
HGNC: HGNC:2366
Homologene: 10899
Chd8
Name: chromodomain helicase DNA binding protein 8
Synonyms: 5830451P18Rik, Duplin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 67772
Homologene: 72405
Adgrl1
Name: adhesion G protein-coupled receptor L1
Synonyms: Lec2, lectomedin-2, 2900070I05Rik, Lphn1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 330814
Homologene: 8951
Zdhhc6
Name: zinc finger, DHHC domain containing 6
Synonyms: 5930409M18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 66980
VEGA: 19
Homologene: 41487
Spag9
Name: sperm associated antigen 9
Synonyms: 4733401I23Rik, syd1, 4831406C20Rik, Mapk8ip4, JIP4, 3110018C07Rik, JLP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 70834
Homologene: 2954
Calcoco1
Name: calcium binding and coiled coil domain 1
Synonyms: 1810009B06Rik, CoCoA, Gcap11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 67488
Homologene: 10845
Nsun2
Name: NOL1/NOP2/Sun domain family member 2
Synonyms: Misu
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 28114
Homologene: 9817
Homer3
Name: homer scaffolding protein 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 26558
Homologene: 37972
Dync2i1
Name: dynein 2 intermediate chain 1
Synonyms: D430033N04Rik, Wdr60, Dync2l1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 217935
VEGA: 12
Homologene: 23067
Myo7a
Name: myosin VIIA
Synonyms: Myo7, USH1B, nmf371, polka, Hdb
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 17921
HGNC: HGNC:7606
Homologene: 219
Cdkl2
Name: cyclin-dependent kinase-like 2 (CDC2-related kinase)
Synonyms: Kkm, KKIAMRE, 5330436L21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 53886
HGNC: HGNC:1782
Homologene: 55793
Ttn
Name: titin
Synonyms: connectin, L56, mdm, 1100001C23Rik, D830007G01Rik, 2310036G12Rik, 2310074I15Rik, 2310057K23Rik, D330041I19Rik, shru
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 22138
Homologene: 130650
Setd1b
Name: SET domain containing 1B
Synonyms: KMT2G
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 208043
Homologene: 134654
Elmod1
Name: ELMO/CED-12 domain containing 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 270162
VEGA: 9
Homologene: 10280
Cplane1
Name: ciliogenesis and planar polarity effector 1
Synonyms: b2b012Clo, Jbts17, Hug, 2410089E03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 73692
Homologene: 11315
Klkb1
Name: kallikrein B, plasma 1
Synonyms: Kal-3, Kal3, Klk3, PSA
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 16621
HGNC: HGNC:6371
Homologene: 68097
Vmn2r59
Name: vomeronasal 2, receptor 59
Synonyms: EG628444
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 628444
Homologene: 129683
Vmn2r82
Name: vomeronasal 2, receptor 82
Synonyms: EG624845
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 624845
Homologene: 83483
Sacs
Name: sacsin
Synonyms: E130115J16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 50720
Cyp4a12a
Name: cytochrome P450, family 4, subfamily a, polypeptide 12a
Synonyms: Cyp4a12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 277753
Homologene: 134044
Olfr356
Name: olfactory receptor 356
Synonyms: GA_x6K02T2NLDC-33631647-33632594, MOR134-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258617
Homologene: 85948
Igsf9b
Name: immunoglobulin superfamily, member 9B
Synonyms: LOC235086, AI414108
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235086
Homologene: 19472
Arl6
Name: ADP-ribosylation factor-like 6
Synonyms: 2210411E14Rik, 1110018H24Rik, BBS3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 56297
Homologene: 12960
Dcbld2
Name: discoidin, CUB and LCCL domain containing 2
Synonyms: 1700055P21Rik, Esdn, CLCP1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 73379
Homologene: 12499
Cfap45
Name: cilia and flagella associated protein 45
Synonyms: 1700028D05Rik, Nesg1, Ccdc19
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 71870
Homologene: 71837
Ip6k3
Name: inositol hexaphosphate kinase 3
Synonyms: D830007E07Rik, Ihpk3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 271424
Homologene: 65257
Pabpc2
Name: poly(A) binding protein, cytoplasmic 2
Synonyms: Pabp2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 18459
Homologene: 56509
Cyp17a1
Name: cytochrome P450, family 17, subfamily a, polypeptide 1
Synonyms: p450c17, Cyp17, steroid 17-alpha hydroxylase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 13074
HGNC: HGNC:2593
Homologene: 73875
Olfr694
Name: olfactory receptor 694
Synonyms: GA_x6K02T2PBJ9-9067220-9066273, MOR283-9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 258444
Homologene: 79345
Adgrg6
Name: adhesion G protein-coupled receptor G6
Synonyms: LOC215798, 1190004A11Rik, DREG, Gpr126
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 215798
Homologene: 10724
Ankrd13b
Name: ankyrin repeat domain 13b
Synonyms: B930093C12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 268445
Homologene: 27367
Slc2a13
Name: solute carrier family 2 (facilitated glucose transporter), member 13
Synonyms: A630029G22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 239606
Homologene: 43139
Slc25a54
Name: solute carrier family 25, member 54
Synonyms: 4930443G12Rik, SCaMC-1like
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 74686
Homologene: 82464
Flacc1
Name: flagellum associated containing coiled-coil domains 1
Synonyms: 4933405P16Rik, 4933425F06Rik, Als2cr12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 108812
Homologene: 51399
Olfr538
Name: olfactory receptor 538
Synonyms: GA_x6K02T2PBJ9-42723314-42724246, MOR253-13P, MOR253-12P, MOR253-10P, MOR253-13P, MOR253-12P, Olfr1553-ps1, Olfr1523-ps1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 258201
Homologene: 110491
Olfr574
Name: olfactory receptor 574
Synonyms: GA_x6K02T2PBJ9-5659738-5660748, MOR14-9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 258357
Homologene: 27137
Vmn1r11
Name: vomeronasal 1 receptor 11
Synonyms: V1rc3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 113860
Homologene: 128345
Cysltr2
Name: cysteinyl leukotriene receptor 2
Synonyms: CYSLT2R, Cyslt2, CysLT2, 2300001H05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 70086
VEGA: 14
Homologene: 10688
H4c12
Name: H4 clustered histone 12
Synonyms: Hist1h4k
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 319160
HGNC: HGNC:4788
Homologene: 134461
Lrit3
Name: leucine-rich repeat, immunoglobulin-like and transmembrane domains 3
Synonyms: LOC242235
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 242235
Homologene: 19426
Gm21786
Name: predicted gene, 21786
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Nscme3l
Name: NSE3 homolog, SMC5-SMC6 complex component like
Synonyms: 1700020D05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 75555
Homologene: 137364
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 58,666,921 bp
  • G to A, chromosome 1 at 172,535,227 bp
  • T to G, chromosome 2 at 36,937,551 bp
  • A to G, chromosome 2 at 76,784,833 bp
  • A to G, chromosome 2 at 76,856,866 bp
  • A to G, chromosome 2 at 104,110,818 bp
  • G to A, chromosome 3 at 109,098,668 bp
  • A to T, chromosome 3 at 129,788,531 bp
  • A to G, chromosome 4 at 33,499,035 bp
  • G to A, chromosome 4 at 115,326,783 bp
  • T to C, chromosome 5 at 8,973,643 bp
  • G to A, chromosome 5 at 92,020,309 bp
  • T to C, chromosome 5 at 121,525,228 bp
  • G to T, chromosome 5 at 123,148,104 bp
  • C to T, chromosome 6 at 46,759,851 bp
  • T to C, chromosome 6 at 50,043,630 bp
  • T to G, chromosome 6 at 57,137,530 bp
  • C to T, chromosome 7 at 42,042,450 bp
  • T to C, chromosome 7 at 98,066,404 bp
  • T to C, chromosome 7 at 102,948,798 bp
  • T to A, chromosome 7 at 106,689,146 bp
  • C to A, chromosome 7 at 140,574,389 bp
  • C to T, chromosome 8 at 45,277,055 bp
  • G to A, chromosome 8 at 70,290,143 bp
  • T to C, chromosome 8 at 83,934,860 bp
  • A to G, chromosome 9 at 27,334,252 bp
  • T to A, chromosome 9 at 53,934,129 bp
  • T to G, chromosome 9 at 67,982,495 bp
  • C to T, chromosome 9 at 71,869,063 bp
  • A to G, chromosome 10 at 14,409,763 bp
  • T to C, chromosome 10 at 25,443,803 bp
  • A to C, chromosome 10 at 79,379,040 bp
  • G to A, chromosome 11 at 4,488,143 bp
  • A to T, chromosome 11 at 6,265,770 bp
  • G to A, chromosome 11 at 77,471,441 bp
  • C to G, chromosome 11 at 94,097,253 bp
  • G to A, chromosome 12 at 116,207,715 bp
  • T to C, chromosome 13 at 13,709,564 bp
  • A to T, chromosome 13 at 13,998,395 bp
  • T to C, chromosome 13 at 21,750,343 bp
  • G to A, chromosome 13 at 69,629,721 bp
  • T to C, chromosome 13 at 80,889,063 bp
  • A to T, chromosome 14 at 52,204,527 bp
  • A to G, chromosome 14 at 61,204,686 bp
  • G to A, chromosome 14 at 73,029,893 bp
  • A to G, chromosome 15 at 8,252,188 bp
  • A to G, chromosome 15 at 91,350,020 bp
  • A to G, chromosome 15 at 102,715,740 bp
  • A to T, chromosome 16 at 17,103,600 bp
  • A to G, chromosome 16 at 58,461,007 bp
  • A to T, chromosome 16 at 59,624,313 bp
  • A to G, chromosome 17 at 27,145,102 bp
  • T to C, chromosome 18 at 39,774,200 bp
  • A to T, chromosome 19 at 5,502,994 bp
  • A to T, chromosome 19 at 41,963,933 bp
  • A to G, chromosome 19 at 46,668,023 bp
  • T to C, chromosome 19 at 55,302,737 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4445 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041151-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.