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Tg(APPswe,PSEN1dE9)85Dbo: Two expression plasmids (Mo/HuAPP695swe and PS1-dE9) were designed to each be controlled by independent mouse prion protein (PrP) promoter elements, directing transgene expression predominantly to CNS neurons. The Mo/HuAPP695swe transgene expresses a humanized mouse amyloid beta (A4) precursor protein gene, modified at three amino acids to reflect the human residues and further modified to contain the K595N/M596L (otherwise known as K670N/M671L) mutations linked to familial Alzheimers. The PS1-dE9 transgene expresses a mutant human presenilin 1 carrying the exon-9-deleted variant (PSEN1dE9) associated with familial Alzheimer.
Nfatc2tm1Rao: A neo cassette was inserted into an exon encoding amino acids near amino-terminus of the DNA binding domain.
Note: The mice are homozygous for the NFATc2 knockout but heterozygous for the APP/PS1 genes since homozygous APP/PS1 mice do not survive.
These mice still deposit robust Abeta peptide containing plaques in the brain but do not have the characteristic reactive microglial or astrocyte activation associated with plaque deposition.
To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.
The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.
Manocha GD, Ghatak A, Puig KL, Kraner SD, Norris CM, Combs CK. NFATc2Modulates Microglial Activation in the AβPP/PS1 Mouse Model of Alzheimer'sDisease. J Alzheimers Dis. 2017;58(3):775-787. doi: 10.3233/JAD-151203. (Medline PMID: 28505967)
NFATc2KO (Nfatc2tm1Rao) sibling females (MMRRC:197) were bred to multiple non-sibling APP/PS1 (B6.Cg-Tg(APPswe,PSEN1dE9)85Dbo/Mmjax - JAX Strain #005864) males. The F1 offspring heterozygous for both traits were then bred to each other to produce F2 mice homozygous for Nfatc2tm1Rao and hemizygous for the transgene.
Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain's tissue samples taken when archived by or ordered from the assigned MMRRC Center.
Colony Surveillance Program and Current Health Reports
Note: All mice are homozygous for the Nfatc2tm1Rao allele
When this strain becomes available, Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
The submitter or their institution limits the distribution to non-profit institutions only.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.
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