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Availability & Fees Order this Strain
The Fgfr1tm8.1Sor allele consists of several amino acid substitutions (L423A, V429A, Y463F, Y766F, Y776F) such that FGFR1 cannot bind to either the Frs2, CrkL, Plc-gamma, or Grb14 adaptors. A targeting vector carrying the Fgfr1tm8.1Sor mutation was generated by cloning a 5′ homology arm (spanning exon 9) and a 3′ homology arm (spanning exons 10–18) into PGKneolox2DTA.
Homozygous: Embryonic lethal around E9.5 with a variety of defects.
Heterozygous: Mice are viable and fertile.
The targeting vector was electroporated into 129S4 AK7 ES cells. ES cell chimeras were bred to Meox2tm1(Cre)Sor mice maintained on a 129S4 genetic background to remove the neomycin selection cassette. The Meox2tm1(Cre)Sor allele was subsequently crossed out. The Fgfr1tm8.1Sor allele was maintained on the 129S4 genetic background.
Brewer JR, Molotkov A, Mazot P, Hoch RV and Soriano P. Fgfr1 regulates development through the combinatorial use of signaling proteins. Genes Dev. 2015 Sep 1;29(17):1863-74. (Medline PMID: 26341559)
Colony Surveillance Program and Current Health Reports
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
The donor or their institution limits the distribution to non-profit institutions only.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.