Strain Name:
C57BL/6J-MtgxR4459Btlr/Mmmh
Stock Number:
041579-MU
Citation ID:
RRID:MMRRC_041579-MU
Other Names:
R4459 (G1), C57BL/6J-MtgxR4459Btlr
Major Collection:

Gene Information

Pou2f1
Name: POU domain, class 2, transcription factor 1
Synonyms: Otf-1, Oct-1A, oct-1, Oct-1C, Oct1, Otf1, Oct-1B, Oct-1z, 2810482H01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 18986
HGNC: HGNC:9212
Homologene: 37658
Kcnj5
Name: potassium inwardly-rectifying channel, subfamily J, member 5
Synonyms: GIRK4, Kir3.4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 16521
VEGA: 9
HGNC: HGNC:6266
Homologene: 20248
Abcc4
Name: ATP-binding cassette, sub-family C (CFTR/MRP), member 4
Synonyms: MRP4, MOAT-B, D630049P08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 239273
VEGA: 14
HGNC: HGNC:55
Homologene: 74563
Snx30
Name: sorting nexin family member 30
Synonyms: 4732481H14Rik, C030041J06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 209131
Homologene: 2811
Dmxl1
Name: Dmx-like 1
Synonyms: C630007L23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 240283
HGNC: HGNC:2937
Homologene: 21136
Ash1l
Name: ASH1 like histone lysine methyltransferase
Synonyms: 8030453L17Rik, KMT2H, E430018P19Rik, chromatin remodeling factor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 192195
Homologene: 10225
Rasal2
Name: RAS protein activator like 2
Synonyms: A330066M24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 226525
HGNC: HGNC:9874
Homologene: 35217
Lrfn4
Name: leucine rich repeat and fibronectin type III domain containing 4
Synonyms: SALM3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 225875
Homologene: 36415
Rrm2b
Name: ribonucleotide reductase M2 B (TP53 inducible)
Synonyms: p53R2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 382985
Homologene: 56723
Klhl26
Name: kelch-like 26
Synonyms: C630013N10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234378
Homologene: 41247
Itga2
Name: integrin alpha 2
Synonyms: VLA-2 receptor, alpha 2 subunit, DX5, CD49B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 16398
VEGA: 13
HGNC: HGNC:6137
Homologene: 1662
Stxbp2
Name: syntaxin binding protein 2
Synonyms: C79054, Munc-18b, Sxtp2, muSec1, Munc18b, Munc-18-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 20911
Homologene: 55530
Efcab6
Name: EF-hand calcium binding domain 6
Synonyms: 4931407K02Rik, 4932408N08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 77627
Homologene: 11259
Arsi
Name: arylsulfatase i
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 545260
Homologene: 44428
Zfp804b
Name: zinc finger protein 804B
Synonyms: LOC207618
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 207618
Homologene: 52304
Rmnd5a
Name: required for meiotic nuclear division 5 homolog A
Synonyms: Gid2, 1110007A06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 68477
Homologene: 5668
Guf1
Name: GUF1 homolog, GTPase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231279
Homologene: 6505
Olfr898
Name: olfactory receptor 898
Synonyms: GA_x6K02T2PVTD-32037624-32038565, MOR170-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 258871
VEGA: 9
Homologene: 133654
Letm2
Name: leucine zipper-EF-hand containing transmembrane protein 2
Synonyms: D030041N04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 270035
Homologene: 56358
Wdr90
Name: WD repeat domain 90
Synonyms: 3230401M21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 106618
Homologene: 27066
Mypn
Name: myopalladin
Synonyms: 1110056A04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 68802
VEGA: 10
Homologene: 23778
Smg8
Name: smg-8 homolog, nonsense mediated mRNA decay factor (C. elegans)
Synonyms: 1200011M11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 74133
Homologene: 32393
Hes6
Name: hairy and enhancer of split 6
Synonyms: bHLHb41
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 55927
Homologene: 23111
Gm15446
Name: predicted gene 15446
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 100642166
BC049715
Name: cDNA sequence BC049715
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 320135
Homologene: 52193
Tyk2
Name: tyrosine kinase 2
Synonyms: JTK1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 54721
VEGA: 9
Homologene: 20712
Olfr1391
Name: olfactory receptor 1391
Synonyms: MOR256-27, GA_x6K02T2QP88-6107233-6106298
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 258460
Homologene: 17264
Gc
Name: vitamin D binding protein
Synonyms: DBP, vitamin D binding protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 14473
HGNC: HGNC:4187
Homologene: 486
Prl7a2
Name: prolactin family 7, subfamily a, member 2
Synonyms: PLP-F, Prlpf
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 19114
Homologene: 49263
Olfr112
Name: olfactory receptor 112
Synonyms: GA_x6K02T2PSCP-2023769-2022807, 4930580F03Rik, MOR218-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 258096
Olfr1110
Name: olfactory receptor 1110
Synonyms: MOR172-4, GA_x6K02T2Q125-48621299-48620361
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258765
Anks1b
Name: ankyrin repeat and sterile alpha motif domain containing 1B
Synonyms: LOC380650, C030032C09Rik, AIDA-1b, Gm10937, E530015N03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 77531
Homologene: 51570
Cyp11b1
Name: cytochrome P450, family 11, subfamily b, polypeptide 1
Synonyms: Cyp11b-1, Cyp11b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 110115
Homologene: 106948
Dnaaf6
Name: dynein axonemal assembly factor 6
Synonyms: 4930521A18Rik, Pih1d3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 74708
Homologene: 16553
9130019O22Rik
Name: RIKEN cDNA 9130019O22 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 78921
Homologene: 118005
Pcdhgb6
Name: protocadherin gamma subfamily B, 6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 93703
HGNC: HGNC:8713
Homologene: 49573
Ighg2b
Name: immunoglobulin heavy constant gamma 2B
Synonyms: gamma2b, IgG2b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 16016
Gm21786
Name: predicted gene, 21786
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Olfr1038-ps
Name: olfactory receptor 1038, pseudogene
Synonyms: GA_x6K02T2Q125-47591072-47592031, MOR185-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 259015
Homologene: 17463
Esp3
Name: exocrine gland secreted peptide 3
Synonyms: Gm21945
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 100126766
VEGA: 17
Mettl4-ps1
Name: methyltransferase like 4, pseudogene 1
Synonyms: ENSMUSG00000079356
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 668740
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 31,223,324 bp
  • A to G, chromosome 1 at 91,412,339 bp
  • A to G, chromosome 1 at 157,175,832 bp
  • A to G, chromosome 1 at 165,895,006 bp
  • T to C, chromosome 2 at 86,122,825 bp
  • T to C, chromosome 2 at 87,135,661 bp
  • G to A, chromosome 3 at 88,966,234 bp
  • A to T, chromosome 4 at 59,885,022 bp
  • A to T, chromosome 5 at 6,771,481 bp
  • C to T, chromosome 5 at 69,565,493 bp
  • T to C, chromosome 5 at 89,441,287 bp
  • A to T, chromosome 5 at 109,943,241 bp
  • A to T, chromosome 6 at 50,043,391 bp
  • C to T, chromosome 6 at 71,396,881 bp
  • G to A, chromosome 6 at 136,840,052 bp
  • A to G, chromosome 7 at 127,384,745 bp
  • C to A, chromosome 8 at 3,635,518 bp
  • A to G, chromosome 8 at 25,586,699 bp
  • T to C, chromosome 8 at 70,451,544 bp
  • T to C, chromosome 9 at 21,124,415 bp
  • A to G, chromosome 9 at 32,322,395 bp
  • T to A, chromosome 9 at 38,349,992 bp
  • A to G, chromosome 10 at 63,192,432 bp
  • G to A, chromosome 10 at 90,510,844 bp
  • A to T, chromosome 11 at 49,327,876 bp
  • T to C, chromosome 11 at 87,085,570 bp
  • T to C, chromosome 12 at 113,306,958 bp
  • A to G, chromosome 13 at 27,665,996 bp
  • C to G, chromosome 13 at 114,843,483 bp
  • C to T, chromosome 14 at 118,599,393 bp
  • T to A, chromosome 15 at 37,945,153 bp
  • T to A, chromosome 15 at 74,836,359 bp
  • C to T, chromosome 15 at 83,904,289 bp
  • T to A, chromosome 17 at 25,861,002 bp
  • G to A, chromosome 17 at 37,563,474 bp
  • C to G, chromosome 17 at 40,633,587 bp
  • T to A, chromosome 17 at 71,556,513 bp
  • C to T, chromosome 18 at 37,742,706 bp
  • A to T, chromosome 18 at 49,961,216 bp
  • G to A, chromosome 18 at 60,916,651 bp
  • C to T, chromosome 19 at 4,612,634 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4459 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041579-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.