Strain Name:
C57BL/6J-MtgxR4465Btlr/Mmmh
Stock Number:
041580-MU
Citation ID:
RRID:MMRRC_041580-MU
Other Names:
R4465 (G1), C57BL/6J-MtgxR4465Btlr
Major Collection:

Gene Information

Atrn
Name: attractin
Synonyms: Mgca
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 11990
HGNC: HGNC:885
Homologene: 22542
Clasp1
Name: CLIP associating protein 1
Synonyms: 5730583A19Rik, 1700030C23Rik, CLASP1alpha, B130045P17Rik, mCLASP1, CLASP1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 76707
Homologene: 41024
Ndnf
Name: neuron-derived neurotrophic factor
Synonyms: A930038C07Rik, epidermacan
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 68169
Homologene: 11593
Erbin
Name: Erbb2 interacting protein
Synonyms: 1700028E05Rik, Erbb2ip, Erbin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 59079
Homologene: 41282
Dis3
Name: DIS3 homolog, exosome endoribonuclease and 3'-5' exoribonuclease
Synonyms: 2810028N01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 72662
VEGA: 14
Homologene: 6910
Lrrk2
Name: leucine-rich repeat kinase 2
Synonyms: 9330188B09Rik, D630001M17Rik, 4921513O20Rik, cI-46, LOC381026
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 66725
Homologene: 18982
Acsbg2
Name: acyl-CoA synthetase bubblegum family member 2
Synonyms: Bgr
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 328845
Homologene: 57133
Cldn8
Name: claudin 8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 54420
HGNC: HGNC:2050
Homologene: 8117
Klk12
Name: kallikrein related-peptidase 12
Synonyms: 2310008B01Rik, KLK-L5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 69511
HGNC: HGNC:6360
Homologene: 44690
Eps15
Name: epidermal growth factor receptor pathway substrate 15
Synonyms: 2410112D09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 13858
HGNC: HGNC:3419
Homologene: 128359
Zfpm2
Name: zinc finger protein, multitype 2
Synonyms: B330005D23Rik, FOG2, FOG-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 22762
VEGA: 15
Homologene: 8008
Col12a1
Name: collagen, type XII, alpha 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 12816
HGNC: HGNC:2188
Homologene: 3217
Dnah11
Name: dynein, axonemal, heavy chain 11
Synonyms: b2b1279Clo, Dnahc11, b2b598Clo, lrd, b2b1727Clo, b2b1203Clo, b2b1289Clo, avc4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 13411
HGNC: HGNC:2942
Homologene: 2801
Adgrb3
Name: adhesion G protein-coupled receptor B3
Synonyms: A830096D10Rik, Bai3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 210933
HGNC: HGNC:945
Homologene: 1289
Rab19
Name: RAB19, member RAS oncogene family
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 19331
Homologene: 22623
Slc22a29
Name: solute carrier family 22. member 29
Synonyms: D630002G06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 236293
Homologene: 77136
Cyp4f40
Name: cytochrome P450, family 4, subfamily f, polypeptide 40
Synonyms: EG631304
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 631304
VEGA: 17
Homologene: 117991
Slx4
Name: SLX4 structure-specific endonuclease subunit homolog (S. cerevisiae)
Synonyms: Btbd12, D16Bwg1016e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 52864
Homologene: 23770
Olfr1097
Name: olfactory receptor 1097
Synonyms: MOR206-2, GA_x6K02T2Q125-48376288-48375341
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258840
Homologene: 37005
Snx25
Name: sorting nexin 25
Synonyms: SBBI31, LOC382008
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 102141
Homologene: 23744
Map3k6
Name: mitogen-activated protein kinase kinase kinase 6
Synonyms: MAPKKK6, Ask2, MEKK6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 53608
HGNC: HGNC:6858
Homologene: 3435
Mup6
Name: major urinary protein 6
Synonyms: Gm12544
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 620807
Homologene: 74304
Lao1
Name: L-amino acid oxidase 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 100470
Homologene: 44223
Slc5a1
Name: solute carrier family 5 (sodium/glucose cotransporter), member 1
Synonyms: sodium glucose cotransporter 1, Sglt1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 20537
Homologene: 55456
F11
Name: coagulation factor XI
Synonyms: plasma thromboplastin antecedent, Cf11, 1600027G01Rik, FXI
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 109821
HGNC: HGNC:3529
Homologene: 86654
0610040J01Rik
Name: RIKEN cDNA 0610040J01 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 76261
Homologene: 49537
Aldh18a1
Name: aldehyde dehydrogenase 18 family, member A1
Synonyms: Pycs, 2810433K04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 56454
HGNC: HGNC:9722
Homologene: 2142
Olfr1084
Name: olfactory receptor 1084
Synonyms: MOR192-2, GA_x6K02T2Q125-48121788-48120847, MOR192-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258235
Homologene: 104285
Slc7a11
Name: solute carrier family 7 (cationic amino acid transporter, y+ system), member 11
Synonyms: System xc, 9930009M05Rik, sut, xCT, xc
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 26570
Homologene: 22684
Olfr441
Name: olfactory receptor 441
Synonyms: MOR261-3, GA_x6K02T2P3E9-4442577-4441645
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 258649
Homologene: 88438
Gsdmcl1
Name: gasdermin C-like 1
Synonyms: Gsdmdc2, 1700022L20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 74236
Sdc2
Name: syndecan 2
Synonyms: 4833414L08Rik, Synd2, fibroglycan, Hspg1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 15529
Homologene: 2253
Zdhhc22
Name: zinc finger, DHHC-type containing 22
Synonyms: LOC238331
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 238331
VEGA: 12
Homologene: 45486
Actg1
Name: actin, gamma, cytoplasmic 1
Synonyms: Actl, E51
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 11465
HGNC: HGNC:144
Homologene: 74402
Tmem181a
Name: transmembrane protein 181A
Synonyms: C76977, 5930418K15Rik, Gpr178, Tmem181
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 77106
Homologene: 44787
Tas2r107
Name: taste receptor, type 2, member 107
Synonyms: T2R07, mt2r43, mGR06, STC 5-1, T2R4, Tas2r7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 387342
Homologene: 137369
Gm11541
Name: predicted gene 11541
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 432589
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to T, chromosome 1 at 25,094,366 bp
  • C to A, chromosome 1 at 118,561,078 bp
  • A to T, chromosome 2 at 86,639,134 bp
  • A to T, chromosome 2 at 86,891,150 bp
  • A to G, chromosome 2 at 130,960,468 bp
  • C to T, chromosome 3 at 50,435,416 bp
  • T to C, chromosome 4 at 60,004,000 bp
  • G to A, chromosome 4 at 109,366,530 bp
  • C to A, chromosome 4 at 118,965,307 bp
  • A to G, chromosome 4 at 133,246,333 bp
  • A to G, chromosome 5 at 33,146,516 bp
  • G to T, chromosome 5 at 63,898,839 bp
  • T to C, chromosome 6 at 39,388,126 bp
  • T to C, chromosome 6 at 43,115,918 bp
  • T to A, chromosome 6 at 65,704,196 bp
  • A to G, chromosome 6 at 131,660,009 bp
  • A to T, chromosome 7 at 43,773,383 bp
  • T to A, chromosome 8 at 45,241,474 bp
  • A to G, chromosome 8 at 46,068,229 bp
  • C to A, chromosome 9 at 79,672,910 bp
  • A to T, chromosome 11 at 94,704,222 bp
  • G to A, chromosome 11 at 120,348,032 bp
  • G to A, chromosome 12 at 86,988,223 bp
  • T to C, chromosome 12 at 117,987,451 bp
  • T to C, chromosome 13 at 103,844,885 bp
  • G to A, chromosome 14 at 99,084,114 bp
  • T to A, chromosome 15 at 32,921,238 bp
  • T to G, chromosome 15 at 41,096,161 bp
  • T to C, chromosome 15 at 63,849,764 bp
  • A to G, chromosome 15 at 91,747,820 bp
  • A to G, chromosome 16 at 3,989,055 bp
  • A to C, chromosome 16 at 88,562,731 bp
  • T to A, chromosome 17 at 6,295,786 bp
  • T to A, chromosome 17 at 32,671,212 bp
  • A to G, chromosome 17 at 56,861,580 bp
  • A to T, chromosome 19 at 8,162,724 bp
  • G to A, chromosome 19 at 40,557,667 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4465 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041580-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.