Strain Name:
C57BL/6J-MtgxR4035Btlr/Mmmh
Stock Number:
041613-MU
Citation ID:
RRID:MMRRC_041613-MU
Other Names:
R4035 (G1), C57BL/6J-MtgxR4035Btlr
Major Collection:

Gene Information

Zfa-ps
Name: zinc finger protein, autosomal, pseudogene
Synonyms: Zfa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 22639
Ptch1
Name: patched 1
Synonyms: Ptc1, Patched 1, A230106A15Rik, wig, Ptc
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 19206
HGNC: HGNC:9585
Homologene: 223
Trpc2
Name: transient receptor potential cation channel, subfamily C, member 2
Synonyms: trp2, 3010009O07Rik, TRPC2a, mTrp2, Trrp2, TRPC2b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 22064
Homologene: 135989
Ifi203
Name: interferon activated gene 203
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 15950
HGNC: HGNC:5395
Homologene: 115929
Ctcf
Name: CCCTC-binding factor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 13018
Homologene: 4786
Ccdc88c
Name: coiled-coil domain containing 88C
Synonyms: Daple, 0610010D24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 68339
VEGA: 12
Homologene: 18903
Utp20
Name: UTP20 small subunit processome component
Synonyms: DRIM, 3830408P06Rik, mDRIM
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 70683
VEGA: 10
Homologene: 38373
Cwf19l2
Name: CWF19-like 2, cell cycle control (S. pombe)
Synonyms: 3230401L03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 244672
Homologene: 12366
Hcn4
Name: hyperpolarization-activated, cyclic nucleotide-gated K+ 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 330953
VEGA: 9
Homologene: 3997
Prtg
Name: protogenin
Synonyms: Igdcc5, A230098A12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 235472
Homologene: 54453
Hmgcr
Name: 3-hydroxy-3-methylglutaryl-Coenzyme A reductase
Synonyms: 3-hydroxy-3-methylglutaryl-CoA reductase, HMG-CoAR, Red
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 15357
HGNC: HGNC:5006
Homologene: 30994
Patj
Name: PATJ, crumbs cell polarity complex component
Synonyms: Cipp, Inadl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 12695
Homologene: 72199
Kat7
Name: K(lysine) acetyltransferase 7
Synonyms: Hbo1, Hboa, Myst2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 217127
Homologene: 5134
Api5
Name: apoptosis inhibitor 5
Synonyms: AAC-11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 11800
HGNC: HGNC:594
Homologene: 4809
Dop1a
Name: DOP1 leucine zipper like protein A
Synonyms: B130005I07Rik, Dopey1, D9Ertd809e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 320615
Homologene: 26645
Rttn
Name: rotatin
Synonyms: C530033I08Rik, 4921538A15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 246102
VEGA: 18
Homologene: 65275
Cep350
Name: centrosomal protein 350
Synonyms: 4933409L06Rik, 6430546F08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 74081
Homologene: 8879
Ttk
Name: Ttk protein kinase
Synonyms: Esk1, Mps1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 22137
Homologene: 2489
Abcb8
Name: ATP-binding cassette, sub-family B (MDR/TAP), member 8
Synonyms: 4833412N02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 74610
HGNC: HGNC:49
Homologene: 5203
Osbpl2
Name: oxysterol binding protein-like 2
Synonyms: ORP-2, C130070J12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 228983
Homologene: 77324
Srsf4
Name: serine/arginine-rich splicing factor 4
Synonyms: SRp75, 5730499P16Rik, Sfrs4, MNCb-2616
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 57317
Homologene: 25624
Isl2
Name: insulin related protein 2 (islet 2)
Synonyms: islet 2, 3110001N10Rik, islet-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 104360
Homologene: 10730
Nolc1
Name: nucleolar and coiled-body phosphoprotein 1
Synonyms: NOPP140, 3230402K17Rik, P130
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 70769
VEGA: 19
Lcorl
Name: ligand dependent nuclear receptor corepressor-like
Synonyms: Mlr1, A830039H10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 209707
Homologene: 82325
Atp2a3
Name: ATPase, Ca++ transporting, ubiquitous
Synonyms: Serca3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 53313
HGNC: HGNC:813
Homologene: 69131
Snx27
Name: sorting nexin family member 27
Synonyms: ESTM47, 5730552M22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 76742
Homologene: 12797
Hmcn2
Name: hemicentin 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 665700
Homologene: 90772
Ttn
Name: titin
Synonyms: shru, connectin, mdm, 2310057K23Rik, D330041I19Rik, 1100001C23Rik, D830007G01Rik, 2310036G12Rik, L56, 2310074I15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 22138
Homologene: 130650
Ndst4
Name: N-deacetylase/N-sulfotransferase (heparin glucosaminyl) 4
Synonyms: 4930439H17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 64580
Homologene: 11208
Fyco1
Name: FYVE and coiled-coil domain containing 1
Synonyms: 2810409M01Rik, Mem2, ZFYVE7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 17281
Homologene: 11561
Mroh2b
Name: maestro heat-like repeat family member 2B
Synonyms: 4930455B06Rik, Heatr7b2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 223825
VEGA: 15
Homologene: 128807
Prpsap1
Name: phosphoribosyl pyrophosphate synthetase-associated protein 1
Synonyms: PAP39, 5730409F23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 67763
HGNC: HGNC:9466
Homologene: 55687
Adcy2
Name: adenylate cyclase 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 210044
VEGA: 13
HGNC: HGNC:233
Homologene: 75133
Henmt1
Name: HEN1 methyltransferase homolog 1 (Arabidopsis)
Synonyms: 4921515J06Rik, Hen1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 66715
Homologene: 32638
Gsdme
Name: gasdermin E
Synonyms: 4932441K13Rik, Fin15, 2310037D07Rik, Dfna5h, Dfna5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 54722
HGNC: HGNC:2810
Homologene: 3242
Krba1
Name: KRAB-A domain containing 1
Synonyms: A930040G15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 77827
Homologene: 15880
Mfsd2b
Name: major facilitator superfamily domain containing 2B
Synonyms: Gm1964
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 432628
Homologene: 47753
Slc39a10
Name: solute carrier family 39 (zinc transporter), member 10
Synonyms: 2900042E17Rik, Zip10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 227059
Homologene: 34076
Ano5
Name: anoctamin 5
Synonyms: Gdd1, Tmem16e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 233246
Homologene: 100071
Zfp267
Name: zinc finger protein 267
Synonyms: D3Ertd254e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 241944
Homologene: 117700
Gm13084
Name: predicted gene 13084
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 381569
Homologene: 77858
Fnip2
Name: folliculin interacting protein 2
Synonyms: D630023B12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 329679
Homologene: 46417
Etfdh
Name: electron transferring flavoprotein, dehydrogenase
Synonyms: 0610010I20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 66841
HGNC: HGNC:3483
Homologene: 3275
Lztr1
Name: leucine-zipper-like transcriptional regulator, 1
Synonyms: 1200003E21Rik, TCFL2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 66863
HGNC: HGNC:6742
Homologene: 4925
Scel
Name: sciellin
Synonyms: 9230114I02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 64929
VEGA: 14
Homologene: 2850
Gbp10
Name: guanylate-binding protein 10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 626578
Homologene: 128731
1700013D24Rik
Name: RIKEN cDNA 1700013D24 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 76921
Homologene: 135963
Nlrp4f
Name: NLR family, pyrin domain containing 4F
Synonyms: C330026N02Rik, Nalp4f, Nalp-kappa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 97895
VEGA: 13
Homologene: 87252
Olfr878
Name: olfactory receptor 878
Synonyms: GA_x6K02T2PVTD-31600511-31601440, MOR163-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 258794
HGNC: HGNC:8473
Homologene: 17395
Coro2b
Name: coronin, actin binding protein, 2B
Synonyms: E130012P22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 235431
HGNC: HGNC:2256
Homologene: 21228
Ube2z
Name: ubiquitin-conjugating enzyme E2Z
Synonyms: C030047H17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 268470
Homologene: 11319
Sema4g
Name: sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4G
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 26456
Homologene: 22682
Spesp1
Name: sperm equatorial segment protein 1
Synonyms: 4921508E09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 66712
VEGA: 9
Homologene: 49838
Bhlhe41
Name: basic helix-loop-helix family, member e41
Synonyms: 6430520M22Rik, Bhlhb3, DEC2, Sharp1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 79362
Homologene: 137371
Olfr212
Name: olfactory receptor 212
Synonyms: GA_x54KRFPKN04-58149882-58150865, 4931403F16Rik, MOR119-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 258019
Homologene: 74186
Cxcl2
Name: chemokine (C-X-C motif) ligand 2
Synonyms: Scyb, Scyb2, Mgsa-b, MIP-2a, CINC-2a, GROb, Gro2, MIP-2, Mip2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 20310
HGNC: HGNC:4604
Homologene: 117695
Gm16517
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 46,812,074 bp
  • T to C, chromosome 1 at 155,959,795 bp
  • T to A, chromosome 1 at 173,929,474 bp
  • A to T, chromosome 2 at 31,336,612 bp
  • T to G, chromosome 2 at 76,909,821 bp
  • C to T, chromosome 2 at 94,425,613 bp
  • G to A, chromosome 2 at 180,161,560 bp
  • T to G, chromosome 3 at 36,164,840 bp
  • C to T, chromosome 3 at 79,479,501 bp
  • C to T, chromosome 3 at 79,613,711 bp
  • T to C, chromosome 3 at 94,524,244 bp
  • T to C, chromosome 3 at 108,958,685 bp
  • C to T, chromosome 3 at 125,438,736 bp
  • T to A, chromosome 4 at 98,591,920 bp
  • C to T, chromosome 4 at 131,900,102 bp
  • T to A, chromosome 4 at 143,810,456 bp
  • A to G, chromosome 5 at 24,400,621 bp
  • A to T, chromosome 5 at 45,734,041 bp
  • A to T, chromosome 5 at 90,904,413 bp
  • T to A, chromosome 5 at 105,224,458 bp
  • A to G, chromosome 6 at 48,411,680 bp
  • A to T, chromosome 6 at 50,229,448 bp
  • A to G, chromosome 6 at 116,516,629 bp
  • A to G, chromosome 6 at 124,356,920 bp
  • A to G, chromosome 6 at 145,863,028 bp
  • A to G, chromosome 7 at 51,566,485 bp
  • G to A, chromosome 7 at 102,084,504 bp
  • A to T, chromosome 8 at 105,664,157 bp
  • A to T, chromosome 9 at 3,456,803 bp
  • G to A, chromosome 9 at 37,918,641 bp
  • A to G, chromosome 9 at 55,542,470 bp
  • A to G, chromosome 9 at 58,843,889 bp
  • T to A, chromosome 9 at 62,273,036 bp
  • A to G, chromosome 9 at 62,425,789 bp
  • G to T, chromosome 9 at 72,842,709 bp
  • C to A, chromosome 9 at 83,854,837 bp
  • T to C, chromosome 9 at 86,494,433 bp
  • T to C, chromosome 9 at 123,801,283 bp
  • T to A, chromosome 10 at 52,544,540 bp
  • A to G, chromosome 10 at 79,669,365 bp
  • C to T, chromosome 10 at 88,762,806 bp
  • T to G, chromosome 11 at 72,975,177 bp
  • A to T, chromosome 11 at 95,281,939 bp
  • A to G, chromosome 11 at 96,061,067 bp
  • A to T, chromosome 11 at 116,473,008 bp
  • A to G, chromosome 12 at 4,870,578 bp
  • G to A, chromosome 12 at 100,930,524 bp
  • T to G, chromosome 13 at 63,524,959 bp
  • T to C, chromosome 13 at 65,194,007 bp
  • A to T, chromosome 13 at 68,657,465 bp
  • C to G, chromosome 13 at 96,651,063 bp
  • A to G, chromosome 14 at 103,530,004 bp
  • T to C, chromosome 15 at 4,945,148 bp
  • T to C, chromosome 16 at 17,524,467 bp
  • T to C, chromosome 18 at 88,995,653 bp
  • A to T, chromosome 19 at 45,001,414 bp
  • GCA to GCACCA, chromosome 19 at 46,081,358 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4035 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041613-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.