Strain Name:
C57BL/6J-MtgxR4056Btlr/Mmmh
Stock Number:
041617-MU
Citation ID:
RRID:MMRRC_041617-MU
Other Names:
R4056 (G1), C57BL/6J-MtgxR4056Btlr
Major Collection:

Gene Information

Sbf2
Name: SET binding factor 2
Synonyms: B430219L04Rik, SBF2, 4833411B01Rik, mMTMH1, Mtmr13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 319934
HGNC: HGNC:2135
Homologene: 41810
Mast2
Name: microtubule associated serine/threonine kinase 2
Synonyms: Mtssk, MAST205
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 17776
Homologene: 7428
Net1
Name: neuroepithelial cell transforming gene 1
Synonyms: Net1 homolog, mNET1, 9530071N24Rik, 0610025H04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 56349
VEGA: 13
Homologene: 4283
Iqgap2
Name: IQ motif containing GTPase activating protein 2
Synonyms: 4933417J23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 544963
VEGA: 13
HGNC: HGNC:6111
Homologene: 101543
Plekha5
Name: pleckstrin homology domain containing, family A member 5
Synonyms: Ayu21-9, Gt(Ayu21)9Imeg, AMH-Cre, PEPP2, 2810431N21Rik, Tg(AMH-cre)1Flor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 109135
Homologene: 10377
Pcsk9
Name: proprotein convertase subtilisin/kexin type 9
Synonyms: Narc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 100102
Homologene: 17790
Btg1
Name: B cell translocation gene 1, anti-proliferative
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 12226
VEGA: 10
HGNC: HGNC:1130
Homologene: 37521
Abcf1
Name: ATP-binding cassette, sub-family F (GCN20), member 1
Synonyms: Abc50, GCN20, D17Wsu166e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 224742
HGNC: HGNC:70
Homologene: 849
Alms1
Name: ALMS1, centrosome and basal body associated
Synonyms: Alstrom syndrome 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 236266
HGNC: HGNC:428
Homologene: 49406
Gabarapl1
Name: gamma-aminobutyric acid (GABA) A receptor-associated protein-like 1
Synonyms: Apg8l, Atg8l, 3110025G09Rik, 9130422N19Rik, GECI
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 57436
HGNC: HGNC:4068
Homologene: 134420
Cntfr
Name: ciliary neurotrophic factor receptor
Synonyms: Cntfralpha
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 12804
HGNC: HGNC:2170
Homologene: 9619
Cyp1a1
Name: cytochrome P450, family 1, subfamily a, polypeptide 1
Synonyms: P450-1, cytochrome P450 subfamily I, polypeptide 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 13076
VEGA: 9
HGNC: HGNC:2595
Homologene: 68062
Pros1
Name: protein S (alpha)
Synonyms: protein S
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 19128
HGNC: HGNC:9456
Homologene: 264
Ikbkb
Name: inhibitor of kappaB kinase beta
Synonyms: IKKbeta, IKK2, IKK-2, IKK-beta, IKK[b]
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 16150
HGNC: HGNC:5960
Homologene: 7782
Ints2
Name: integrator complex subunit 2
Synonyms: 2810417D08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 70422
Homologene: 10801
Kalrn
Name: kalirin, RhoGEF kinase
Synonyms: 2210407G14Rik, Hapip, E530005C20Rik, LOC224126
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 545156
HGNC: HGNC:4814
Homologene: 57160
Wif1
Name: Wnt inhibitory factor 1
Synonyms: WIF-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 24117
Homologene: 31430
Trpv5
Name: transient receptor potential cation channel, subfamily V, member 5
Synonyms: ECaC1, CaT2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 194352
HGNC: HGNC:3145
Homologene: 10520
Bmper
Name: BMP-binding endothelial regulator
Synonyms: CV-2, Crim3, Cv2, 3110056H04Rik, crossveinless-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 73230
VEGA: 9
Homologene: 12494
Dnah17
Name: dynein, axonemal, heavy chain 17
Synonyms: LOC382552, 2810003K23Rik, Dnahcl1, Dnahc17
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 69926
HGNC: HGNC:2946
Homologene: 72102
Nav3
Name: neuron navigator 3
Synonyms: 9630020C08Rik, Pomfil1p, 4732483H20Rik, unc53H3, steerin 3, POMFIL1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 260315
Homologene: 56688
Gm1966
Name: predicted gene 1966
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 434223
HGNC: null
Homologene: 32708
Rims1
Name: regulating synaptic membrane exocytosis 1
Synonyms: RIM1alpha, RIM1, C030033M19Rik, RIM1a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 116837
Homologene: 128399
4930458L03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
4932425I24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
Mpo
Name: myeloperoxidase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 17523
HGNC: HGNC:7218
Homologene: 55450
Col6a4
Name: collagen, type VI, alpha 4
Synonyms: Dvwa, Vwa6, EG235580, 1110001D15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 68553
HGNC: null
Homologene: 130754
Adamts18
Name: a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 18
Synonyms: E130314N14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 208936
Homologene: 65241
Hpse2
Name: heparanase 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 545291
VEGA: 19
Homologene: 19680
Rxfp2
Name: relaxin/insulin-like family peptide receptor 2
Synonyms: Great, Gpr106, LGR8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 140498
Homologene: 15402
Slc22a23
Name: solute carrier family 22, member 23
Synonyms: 3110004L20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 73102
Homologene: 41457
Fcgbp
Name: Fc fragment of IgG binding protein
Synonyms: A430096B05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 215384
Homologene: 68369
Vmn1r13
Name: vomeronasal 1 receptor 13
Synonyms: V1rc5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 113862
HGNC: null
Homologene: 74368
Zfyve16
Name: zinc finger, FYVE domain containing 16
Synonyms: B130024H06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 218441
Homologene: 8826
Plekhg3
Name: pleckstrin homology domain containing, family G (with RhoGef domain) member 3
Synonyms: MGC40768
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 263406
VEGA: 12
Homologene: 77478
Ctnna3
Name: catenin (cadherin associated protein), alpha 3
Synonyms: 4930429L08Rik, alphaT-catenin, Catna3, Vr22
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 216033
HGNC: HGNC:2511
Homologene: 56583
5730455P16Rik
Name: RIKEN cDNA 5730455P16 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 70591
Homologene: 11177
Hs3st2
Name: heparan sulfate (glucosamine) 3-O-sulfotransferase 2
Synonyms: 6430516N12Rik, A830061E14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 195646
HGNC: HGNC:5195
Homologene: 21220
Rhbg
Name: Rhesus blood group-associated B glycoprotein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 58176
Homologene: 9469
Sesn3
Name: sestrin 3
Synonyms: SEST3, 5630400E15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 75747
Homologene: 14386
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 22,292,939 bp
  • C to T, chromosome 3 at 88,243,448 bp
  • A to G, chromosome 4 at 41,658,900 bp
  • T to A, chromosome 4 at 106,444,702 bp
  • T to A, chromosome 4 at 116,337,501 bp
  • T to C, chromosome 5 at 150,051,633 bp
  • G to A, chromosome 6 at 41,659,705 bp
  • G to A, chromosome 6 at 57,209,985 bp
  • A to G, chromosome 6 at 85,587,803 bp
  • T to C, chromosome 6 at 129,538,630 bp
  • G to T, chromosome 6 at 140,589,232 bp
  • A to T, chromosome 7 at 28,104,116 bp
  • G to T, chromosome 7 at 106,604,009 bp
  • T to C, chromosome 7 at 110,441,466 bp
  • T to A, chromosome 7 at 121,500,702 bp
  • A to T, chromosome 8 at 22,665,978 bp
  • T to A, chromosome 8 at 113,737,580 bp
  • C to T, chromosome 9 at 14,322,716 bp
  • A to G, chromosome 9 at 23,399,629 bp
  • T to A, chromosome 9 at 57,700,149 bp
  • C to A, chromosome 9 at 106,026,466 bp
  • T to C, chromosome 10 at 65,002,568 bp
  • T to A, chromosome 10 at 96,618,354 bp
  • A to T, chromosome 10 at 109,880,533 bp
  • G to A, chromosome 10 at 121,082,194 bp
  • T to A, chromosome 11 at 80,375,503 bp
  • A to C, chromosome 11 at 86,242,952 bp
  • T to C, chromosome 11 at 87,795,187 bp
  • T to C, chromosome 11 at 118,070,538 bp
  • T to A, chromosome 12 at 76,565,247 bp
  • G to A, chromosome 13 at 3,884,949 bp
  • A to C, chromosome 13 at 34,299,004 bp
  • T to A, chromosome 13 at 64,921,655 bp
  • T to C, chromosome 13 at 92,504,549 bp
  • A to G, chromosome 13 at 95,750,033 bp
  • A to T, chromosome 16 at 34,314,209 bp
  • A to G, chromosome 16 at 38,298,214 bp
  • A to T, chromosome 16 at 62,900,645 bp
  • A to G, chromosome 17 at 35,959,915 bp
  • T to C, chromosome 19 at 43,294,275 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4056 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041617-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.