Strain Name:
C57BL/6J-MtgxR4064Btlr/Mmmh
Stock Number:
041620-MU
Citation ID:
RRID:MMRRC_041620-MU
Other Names:
R4064 (G1), C57BL/6J-MtgxR4064Btlr
Major Collection:

Gene Information

Espl1
Name: extra spindle pole bodies 1, separase
Synonyms: separase, SSE, ESP1, PRCE, PRCE, Cerp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 105988
VEGA: 15
Homologene: 32151
Tmem59l
Name: transmembrane protein 59-like
Synonyms: 5330410G16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 67937
Homologene: 8104
Cdc5l
Name: cell division cycle 5-like (S. pombe)
Synonyms: PCDC5RP, 1200002I02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 71702
VEGA: 17
HGNC: HGNC:1743
Homologene: 13291
Hnrnpll
Name: heterogeneous nuclear ribonucleoprotein L-like
Synonyms: 2510028H02Rik, 2810036L13Rik, Hnrpll
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 72692
Homologene: 26701
Rab11fip3
Name: RAB11 family interacting protein 3 (class II)
Synonyms: Rab11-FIP3, D030060O14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 215445
Homologene: 49396
Fbxo15
Name: F-box protein 15
Synonyms: Fbx15, ecat3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 50764
VEGA: 18
Homologene: 17644
Zfp292
Name: zinc finger protein 292
Synonyms: Zn-16, Zn-15, Krox-10, Zfp-15, 9430062L07Rik, 5730450D02Rik, Zfp15
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 30046
Homologene: 8493
Psmb7
Name: proteasome (prosome, macropain) subunit, beta type 7
Synonyms: MC14
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 19177
HGNC: HGNC:9544
Homologene: 2093
Parp4
Name: poly (ADP-ribose) polymerase family, member 4
Synonyms: p193, PH5P, E230037B21Rik, VPARP, VAULT3, Adprtl1, C030027K23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 328417
HGNC: HGNC:271
Homologene: 124423
Fosb
Name: FBJ osteosarcoma oncogene B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 14282
HGNC: HGNC:3797
Homologene: 31403
Pus10
Name: pseudouridylate synthase 10
Synonyms: 2810013G11Rik, 4933435A13Rik, Ccdc139
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 74467
Homologene: 12566
Rreb1
Name: ras responsive element binding protein 1
Synonyms: 1110037N09Rik, B930013M22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 68750
Homologene: 2218
Mphosph9
Name: M-phase phosphoprotein 9
Synonyms: 4930548D04Rik, MPP-9, MPP9, B930097C17Rik, 9630025B04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 269702
HGNC: HGNC:7215
Homologene: 11256
Zscan22
Name: zinc finger and SCAN domain containing 22
Synonyms: D530006B18Rik, Hkr2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 232878
HGNC: HGNC:4929
Homologene: 68122
Tarsl2
Name: threonyl-tRNA synthetase-like 2
Synonyms: A530046H20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 272396
Homologene: 65036
Zfp282
Name: zinc finger protein 282
Synonyms: HUB1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 101095
Homologene: 2647
Rp1
Name: retinitis pigmentosa 1 (human)
Synonyms: oxygen-regulated protein 1, Orp1, mG145, Dcdc3, Rp1h
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 19888
Homologene: 4564
Mfsd13a
Name: major facilitator superfamily domain containing 13a
Synonyms: 4930449A08Rik, 4930538D17Rik, Tmem180
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 75146
Homologene: 12592
Tbc1d2b
Name: TBC1 domain family, member 2B
Synonyms: 1810061M12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 67016
Homologene: 56694
Otogl
Name: otogelin-like
Synonyms: Gm6924
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 628870
Homologene: 46008
Sh3pxd2b
Name: SH3 and PX domains 2B
Synonyms: G431001E03Rik, Fad49, Tks4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 268396
Homologene: 27952
Axl
Name: AXL receptor tyrosine kinase
Synonyms: Tyro7, Ark, Ufo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 26362
HGNC: HGNC:905
Homologene: 7583
Ago4
Name: argonaute RISC catalytic subunit 4
Synonyms: 5730550L01Rik, argonaute 4, Eif2c4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 76850
Homologene: 41184
Spata20
Name: spermatogenesis associated 20
Synonyms: Tisp78
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 217116
Homologene: 11264
Ccdc30
Name: coiled-coil domain containing 30
Synonyms: 1700111D19Rik, 4930445I03Rik, 1700001O02Rik, 1700041C02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 73332
Homologene: 137391
Dpy19l3
Name: dpy-19-like 3 (C. elegans)
Synonyms: 9330164H19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233115
Homologene: 18692
Abcc2
Name: ATP-binding cassette, sub-family C (CFTR/MRP), member 2
Synonyms: multidrug resistance protein 2, Mrp2, Cmoat
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 12780
HGNC: HGNC:53
Homologene: 68052
Alkbh2
Name: alkB homolog 2, alpha-ketoglutarate-dependent dioxygenase
Synonyms: Abh2, mABH2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231642
Homologene: 18393
Etfdh
Name: electron transferring flavoprotein, dehydrogenase
Synonyms: 0610010I20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 66841
HGNC: HGNC:3483
Homologene: 3275
Rgl2
Name: ral guanine nucleotide dissociation stimulator-like 2
Synonyms: Rlf, KE1.5, Rab2l, Rgt2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 19732
HGNC: HGNC:9769
Homologene: 3494
Afmid
Name: arylformamidase
Synonyms: Kf, formylkynureninase, formylase, kynurenine formamidase, 9030621K19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 71562
Homologene: 41731
Otop2
Name: otopetrin 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 237987
Homologene: 27803
Serpinb7
Name: serine (or cysteine) peptidase inhibitor, clade B, member 7
Synonyms: 4631416M05Rik, megsin, ovalbumin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 116872
Homologene: 68363
Olfr1141
Name: olfactory receptor 1141
Synonyms: GA_x6K02T2Q125-49257818-49256883, MOR177-10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258630
Homologene: 74080
Vmn1r9
Name: vomeronasal 1 receptor 9
Synonyms: V1rc30
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 171203
Homologene: 76459
Slc26a9
Name: solute carrier family 26, member 9
Synonyms: anion transporter/exchanger-9, E030002L01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 320718
Homologene: 14179
Mrps24
Name: mitochondrial ribosomal protein S24
Synonyms: Rpms24, 3110030K20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 64660
Homologene: 57043
Gtpbp2
Name: GTP binding protein 2
Synonyms: nmf205
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 56055
HGNC: HGNC:4670
Homologene: 10420
Olfr1
Name: olfactory receptor 1
Synonyms: MOR135-13, GA_x6K02T2P1NL-3556334-3555390, I54
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 258923
Homologene: 115482
Duoxa2
Name: dual oxidase maturation factor 2
Synonyms: 9030623N16Rik, Nip2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 66811
Homologene: 57037
RP23-105H6.2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Gm6124
Name: predicted gene 6124
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 620018
Homologene: 45597
Nhlh2
Name: nescient helix loop helix 2
Synonyms: Nscl-2, Hen2, NSCL2, 6230401I09Rik, bHLHa34
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 18072
HGNC: HGNC:7818
Homologene: 4090
Gm17511
Name: predicted gene, 17511
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 100502641
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 4,345,400 bp
  • A to T, chromosome 1 at 107,446,036 bp
  • T to C, chromosome 1 at 131,763,187 bp
  • T to C, chromosome 2 at 38,640,176 bp
  • A to G, chromosome 2 at 87,753,789 bp
  • G to T, chromosome 2 at 122,300,577 bp
  • A to G, chromosome 2 at 169,962,325 bp
  • T to C, chromosome 3 at 79,605,791 bp
  • A to G, chromosome 3 at 102,012,736 bp
  • A to G, chromosome 4 at 34,810,863 bp
  • T to C, chromosome 4 at 119,401,007 bp
  • A to T, chromosome 4 at 126,515,862 bp
  • C to T, chromosome 5 at 114,124,226 bp
  • A to T, chromosome 5 at 124,290,917 bp
  • G to A, chromosome 6 at 47,880,094 bp
  • T to C, chromosome 6 at 57,071,321 bp
  • C to T, chromosome 7 at 12,907,014 bp
  • G to T, chromosome 7 at 19,305,192 bp
  • C to A, chromosome 7 at 25,764,020 bp
  • G to A, chromosome 7 at 35,716,617 bp
  • T to A, chromosome 7 at 39,222,806 bp
  • G to A, chromosome 7 at 65,652,270 bp
  • T to A, chromosome 7 at 127,286,045 bp
  • T to C, chromosome 8 at 70,485,719 bp
  • T to A, chromosome 9 at 90,218,922 bp
  • A to T, chromosome 10 at 107,790,649 bp
  • G to A, chromosome 11 at 5,704,676 bp
  • A to G, chromosome 11 at 23,728,983 bp
  • G to T, chromosome 11 at 32,422,263 bp
  • A to T, chromosome 11 at 73,395,522 bp
  • G to A, chromosome 11 at 94,480,554 bp
  • G to A, chromosome 11 at 115,329,375 bp
  • A to G, chromosome 11 at 117,836,528 bp
  • T to C, chromosome 13 at 37,930,317 bp
  • A to G, chromosome 14 at 56,624,140 bp
  • A to G, chromosome 15 at 102,312,989 bp
  • T to C, chromosome 17 at 26,024,394 bp
  • T to A, chromosome 17 at 33,937,108 bp
  • C to T, chromosome 17 at 45,410,890 bp
  • G to A, chromosome 17 at 46,167,327 bp
  • T to C, chromosome 17 at 80,032,772 bp
  • C to T, chromosome 18 at 84,959,118 bp
  • T to G, chromosome 19 at 43,804,993 bp
  • T to C, chromosome 19 at 46,368,272 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4064 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041620-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.