Strain Name:
C57BL/6J-MtgxR4283Btlr/Mmmh
Stock Number:
041651-MU
Citation ID:
RRID:MMRRC_041651-MU
Other Names:
R4283 (G1), C57BL/6J-MtgxR4283Btlr
Major Collection:

Gene Information

Dnmt3a
Name: DNA methyltransferase 3A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 13435
HGNC: HGNC:2978
Homologene: 7294
Slc17a6
Name: solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 6
Synonyms: 2900073D12Rik, VGLUT2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 140919
Homologene: 121617
Hsd17b12
Name: hydroxysteroid (17-beta) dehydrogenase 12
Synonyms: keratonectin, 2610510O05Rik, keratoadhesin, KIK-I
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 56348
Homologene: 95094
Ncaph
Name: non-SMC condensin I complex, subunit H
Synonyms: Brrn1, A730011O11Rik, HCAP-H
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 215387
HGNC: HGNC:1112
Homologene: 133986
Arl6ip1
Name: ADP-ribosylation factor-like 6 interacting protein 1
Synonyms: AIP-6, ARMER
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 54208
HGNC: HGNC:697
Homologene: 41008
Ptpn21
Name: protein tyrosine phosphatase, non-receptor type 21
Synonyms: PTPD1, PTPRL10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 24000
VEGA: 12
HGNC: HGNC:9651
Homologene: 5110
Ric1
Name: RAB6A GEF complex partner 1
Synonyms: C030046E11Rik, C130057E09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 226089
Homologene: 13806
Iqgap3
Name: IQ motif containing GTPase activating protein 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 404710
Homologene: 26400
Upf2
Name: UPF2 regulator of nonsense transcripts homolog (yeast)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 326622
Homologene: 6101
Cd274
Name: CD274 antigen
Synonyms: B7-H1, Pdcd1lg1, PD-L1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 60533
Homologene: 8560
Strip2
Name: striatin interacting protein 2
Synonyms: D330017J20Rik, Fam40b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 320609
Homologene: 66198
6030468B19Rik
Name: RIKEN cDNA 6030468B19 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 77727
Homologene: 90660
Adgrg5
Name: adhesion G protein-coupled receptor G5
Synonyms: LOC382045, PGR27, Gpr114
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 382045
Homologene: 17828
Copg1
Name: coatomer protein complex, subunit gamma 1
Synonyms: Copg, D6Ertd71e, D6Wsu16e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 54161
HGNC: HGNC:2236
Homologene: 56745
Crtc2
Name: CREB regulated transcription coactivator 2
Synonyms: 4632407F12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 74343
Homologene: 18765
Rec8
Name: REC8 meiotic recombination protein
Synonyms: Rec8L1, mrec
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 56739
Homologene: 3768
Tfeb
Name: transcription factor EB
Synonyms: bHLHe35, Tcfeb, TFEB
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 21425
Homologene: 5182
Ghr
Name: growth hormone receptor
Synonyms: GHR/BP, GHBP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 14600
HGNC: HGNC:4263
Homologene: 134
Ttn
Name: titin
Synonyms: mdm, 2310036G12Rik, D830007G01Rik, shru, L56, 2310074I15Rik, 2310057K23Rik, connectin, D330041I19Rik, 1100001C23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 22138
Homologene: 130650
Zfp429
Name: zinc finger protein 429
Synonyms: 2810487A22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 72807
Homologene: 110878
Tas2r123
Name: taste receptor, type 2, member 123
Synonyms: mt2r55, Tas2r23, STC 9-2, mGR23, T2R23
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 353167
Homologene: 45450
Shank2
Name: SH3 and multiple ankyrin repeat domains 2
Synonyms: ProSAP1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 210274
Homologene: 105965
Gm10750
Name: predicted gene 10750
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Gmip
Name: Gem-interacting protein
Synonyms: 5031419I10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 78816
Homologene: 9570
Ninl
Name: ninein-like
Synonyms: LOC381387, LOC381388, 4930519N13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 78177
Homologene: 57024
Tas2r143
Name: taste receptor, type 2, member 143
Synonyms: mt2r36, Tas2r43
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 387514
Homologene: 74285
Vmn2r52
Name: vomeronasal 2, receptor 52
Synonyms: EG384534
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 384534
Homologene: 129605
Tas2r104
Name: taste receptor, type 2, member 104
Synonyms: T2R04, mt2r45, mGR04, Tas2r4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 387340
Homologene: 130072
Hdac10
Name: histone deacetylase 10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 170787
Homologene: 23749
Gm17541
Name: predicted gene, 17541
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 100416664
VEGA: 12
Zfp28
Name: zinc finger protein 28
Synonyms: 2810438M17Rik, Zfp-28, mkr-5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 22690
Homologene: 69047
Pcgf1
Name: polycomb group ring finger 1
Synonyms: Nspc1, 2010002K04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 69837
Homologene: 13090
Pcsk4
Name: proprotein convertase subtilisin/kexin type 4
Synonyms: PC4, SPC5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 18551
HGNC: HGNC:8746
Homologene: 22495
Cplx2
Name: complexin 2
Synonyms: Gm34843, 921-L
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 12890
HGNC: HGNC:2310
Homologene: 38212
Defb40
Name: defensin beta 40
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 360217
Homologene: 86851
Card9
Name: caspase recruitment domain family, member 9
Synonyms: LOC332579
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 332579
Homologene: 14150
Camk2b
Name: calcium/calmodulin-dependent protein kinase II, beta
Synonyms: CaMK II
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 12323
HGNC: HGNC:1461
Homologene: 111050
Defa27
Name: defensin, alpha, 27
Synonyms: ENSMUSG00000074438, Gm15299
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 100041811
Homologene: 113382
Platr9
Name: pluripotency associated transcript 9
Synonyms: 2410114N07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 73696
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 2 at 5,973,558 bp
  • A to G, chromosome 2 at 26,357,297 bp
  • T to A, chromosome 2 at 76,754,824 bp
  • G to C, chromosome 2 at 94,033,586 bp
  • A to G, chromosome 2 at 127,121,085 bp
  • A to G, chromosome 2 at 149,015,996 bp
  • T to C, chromosome 2 at 150,953,416 bp
  • T to C, chromosome 3 at 88,098,860 bp
  • A to G, chromosome 3 at 90,259,236 bp
  • T to C, chromosome 4 at 34,905,264 bp
  • C to T, chromosome 6 at 29,941,937 bp
  • T to A, chromosome 6 at 42,401,073 bp
  • T to A, chromosome 6 at 83,079,733 bp
  • C to T, chromosome 6 at 87,908,545 bp
  • C to T, chromosome 6 at 131,685,411 bp
  • G to A, chromosome 6 at 132,848,045 bp
  • A to T, chromosome 7 at 6,393,701 bp
  • C to T, chromosome 7 at 10,170,638 bp
  • A to G, chromosome 7 at 51,645,076 bp
  • AAAATAAATAAATAAATAAATAAATA to AAAATAAATAAATAAATAAATAAATAAATA, chromosome 7 at 118,121,899 bp
  • C to T, chromosome 7 at 144,395,480 bp
  • A to G, chromosome 8 at 18,978,077 bp
  • A to G, chromosome 8 at 21,315,616 bp
  • T to A, chromosome 8 at 69,813,601 bp
  • T to G, chromosome 8 at 94,937,698 bp
  • C to A, chromosome 10 at 80,329,453 bp
  • A to C, chromosome 11 at 5,987,099 bp
  • A to G, chromosome 11 at 117,806,261 bp
  • G to A, chromosome 12 at 3,901,665 bp
  • A to G, chromosome 12 at 4,689,656 bp
  • T to C, chromosome 12 at 98,733,475 bp
  • A to G, chromosome 13 at 54,379,564 bp
  • A to C, chromosome 13 at 67,390,795 bp
  • A to G, chromosome 14 at 55,618,634 bp
  • C to A, chromosome 15 at 3,333,448 bp
  • A to G, chromosome 15 at 89,125,623 bp
  • A to G, chromosome 17 at 47,789,774 bp
  • A to T, chromosome 19 at 29,380,471 bp
  • A to G, chromosome 19 at 29,586,550 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4283 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041651-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.