Strain Name:
C57BL/6J-MtgxR4300Btlr/Mmmh
Stock Number:
041657-MU
Citation ID:
RRID:MMRRC_041657-MU
Other Names:
R4300 (G1), C57BL/6J-MtgxR4300Btlr
Major Collection:

Gene Information

Setd5
Name: SET domain containing 5
Synonyms: 2900045N06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 72895
Homologene: 12485
Dip2c
Name: disco interacting protein 2 homolog C
Synonyms: 2900024P20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 208440
Homologene: 40996
Pcnt
Name: pericentrin (kendrin)
Synonyms: m275Asp, Pcnt2, m239Asp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 18541
VEGA: 10
Srbd1
Name: S1 RNA binding domain 1
Synonyms: D530025C17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 78586
VEGA: 17
Homologene: 9994
Tfap4
Name: transcription factor AP4
Synonyms: Tcfap4, bHLHc41, AP-4, D930048N17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 83383
VEGA: 16
Homologene: 2424
Prc1
Name: protein regulator of cytokinesis 1
Synonyms: D7Ertd348e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 233406
HGNC: HGNC:9341
Homologene: 37868
Herc1
Name: HECT and RLD domain containing E3 ubiquitin protein ligase family member 1
Synonyms: D130015N03Rik, 2810449H11Rik, tbl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 235439
HGNC: HGNC:4867
Homologene: 31207
Cyth1
Name: cytohesin 1
Synonyms: Pscd1, CLM1, CTH-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 19157
HGNC: HGNC:9501
Homologene: 31262
Mtfr1
Name: mitochondrial fission regulator 1
Synonyms: Fam54a2, 4930579E05Rik, 2810026O10Rik, 1700080D04Rik, 1300002C08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 67472
Homologene: 8763
Tubgcp3
Name: tubulin, gamma complex associated protein 3
Synonyms: Spc98p, GCP3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 259279
Homologene: 4609
Dnajc12
Name: DnaJ heat shock protein family (Hsp40) member C12
Synonyms: Jdp1, mJDP1, J domain protein 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 30045
Homologene: 8492
Spata31
Name: spermatogenesis associated 31
Synonyms: Fam75a, 4930458L03Rik, Spata31a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 78124
Homologene: 84621
Kcnn4
Name: potassium intermediate/small conductance calcium-activated channel, subfamily N, member 4
Synonyms: IKCa1, IK1, SK4, mIKCa1, KCa3.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 16534
HGNC: HGNC:6293
Homologene: 1696
A2m
Name: alpha-2-macroglobulin
Synonyms: A2mp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 232345
HGNC: HGNC:7
Homologene: 37248
Cacna1b
Name: calcium channel, voltage-dependent, N type, alpha 1B subunit
Synonyms: Cchn1a, Cav2.2, alpha(1B)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 12287
HGNC: HGNC:1389
Homologene: 20184
Stox2
Name: storkhead box 2
Synonyms: 4933409N07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 71069
Homologene: 18414
Txlnb
Name: taxilin beta
Synonyms: Mdp77, 2310001N14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 378431
Homologene: 44534
Top2b
Name: topoisomerase (DNA) II beta
Synonyms: Top-2, D230016L12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 21974
Homologene: 134711
Rfx4
Name: regulatory factor X, 4 (influences HLA class II expression)
Synonyms: 4933412G19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 71137
HGNC: HGNC:9985
Homologene: 31119
Pik3cg
Name: phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma
Synonyms: PI3Kgamma, p110gamma, PI3Kgamma, 5830428L06Rik, PI(3)Kgamma
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 30955
HGNC: HGNC:8978
Homologene: 68269
Sun1
Name: Sad1 and UNC84 domain containing 1
Synonyms: 4632417G13Rik, Unc84a, 5730434D03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 77053
Homologene: 11544
Ccs
Name: copper chaperone for superoxide dismutase
Synonyms: CCS, Ccsd
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 12460
VEGA: 19
HGNC: HGNC:1613
Homologene: 3762
Slc14a2
Name: solute carrier family 14 (urea transporter), member 2
Synonyms: UT-A3, UT-A5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 27411
VEGA: 18
Homologene: 5183
Armc2
Name: armadillo repeat containing 2
Synonyms: 2610018I05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 213402
Homologene: 41848
Rmc1
Name: regulator of MON1-CCZ1
Synonyms: 3110002H16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 76482
Homologene: 8341
4930503B20Rik
Name: RIKEN cDNA 4930503B20 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 75015
Homologene: 41344
Lrrc8d
Name: leucine rich repeat containing 8D
Synonyms: 4930525N13Rik, 2810473G09Rik, Lrrc5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 231549
Homologene: 10004
Sirpb1b
Name: signal-regulatory protein beta 1B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 668101
Homologene: 82993
Kcnd3
Name: potassium voltage-gated channel, Shal-related family, member 3
Synonyms: potassium channel Kv4.3M, Kv4.3, potassium channel Kv4.3L
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 56543
HGNC: HGNC:6239
Homologene: 21036
Cd177
Name: CD177 antigen
Synonyms: Pdp3, 1190003K14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 68891
Homologene: 49628
Fuca1
Name: fucosidase, alpha-L- 1, tissue
Synonyms: Afuc, 0610006A03Rik, 9530055J05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 71665
HGNC: HGNC:4006
Homologene: 20078
Olfr981
Name: olfactory receptor 981
Synonyms: GA_x6K02T2PVTD-33720892-33721824, MOR223-8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 258283
VEGA: 9
Homologene: 64849
Mboat2
Name: membrane bound O-acyltransferase domain containing 2
Synonyms: 2810049G06Rik, Oact2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 67216
VEGA: 12
Homologene: 6971
Pcdhgc4
Name: protocadherin gamma subfamily C, 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 93707
HGNC: HGNC:8717
Homologene: 110932
Olfr192
Name: olfactory receptor 192
Synonyms: GA_x54KRFPKG5P-55327126-55326203, MOR183-11_p
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 404309
Homologene: 131129
RP24-282D16.12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
1700119I11Rik
Name: RIKEN cDNA 1700119I11 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 2 at 24,635,239 bp
  • A to T, chromosome 3 at 15,548,761 bp
  • T to A, chromosome 3 at 19,215,457 bp
  • C to T, chromosome 3 at 105,658,766 bp
  • T to A, chromosome 3 at 146,650,920 bp
  • G to A, chromosome 4 at 135,929,950 bp
  • G to A, chromosome 5 at 105,813,740 bp
  • A to T, chromosome 5 at 139,227,594 bp
  • T to G, chromosome 6 at 113,150,162 bp
  • G to A, chromosome 6 at 121,673,475 bp
  • G to T, chromosome 7 at 24,377,604 bp
  • T to C, chromosome 7 at 24,750,420 bp
  • G to A, chromosome 7 at 80,311,216 bp
  • G to T, chromosome 8 at 12,657,600 bp
  • A to T, chromosome 8 at 47,193,992 bp
  • A to C, chromosome 9 at 40,023,139 bp
  • A to G, chromosome 9 at 66,489,406 bp
  • G to A, chromosome 9 at 72,385,476 bp
  • A to G, chromosome 10 at 17,827,925 bp
  • C to A, chromosome 10 at 41,927,056 bp
  • C to A, chromosome 10 at 63,386,730 bp
  • G to C, chromosome 10 at 76,367,391 bp
  • T to C, chromosome 10 at 84,905,102 bp
  • A to G, chromosome 11 at 118,183,894 bp
  • A to G, chromosome 12 at 24,959,083 bp
  • A to G, chromosome 12 at 32,176,672 bp
  • A to G, chromosome 13 at 9,610,711 bp
  • A to T, chromosome 13 at 64,919,761 bp
  • C to A, chromosome 13 at 91,863,338 bp
  • T to G, chromosome 14 at 16,409,189 bp
  • T to C, chromosome 16 at 4,551,360 bp
  • T to C, chromosome 16 at 59,098,278 bp
  • C to A, chromosome 17 at 85,985,204 bp
  • A to G, chromosome 18 at 12,188,862 bp
  • G to A, chromosome 18 at 37,815,826 bp
  • G to A, chromosome 18 at 78,207,068 bp
  • T to C, chromosome 19 at 4,834,257 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4300 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041657-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.