Strain Name:
C57BL/6J-MtgxR4377Btlr/Mmmh
Stock Number:
041676-MU
Citation ID:
RRID:MMRRC_041676-MU
Other Names:
R4377 (G1), C57BL/6J-MtgxR4377Btlr
Major Collection:

Gene Information

Gene Symbol: Kit (Mus musculus (mouse))
Name: KIT proto-oncogene receptor tyrosine kinase
Synonyms: Gsfsow3, Steel Factor Receptor, c-KIT, Gsfsco5, Dominant white spotting, Gsfsco1, belly-spot, Tr-kit, SCO1, SCO5, SOW3, CD117
Chromosome: 5
Alteration at locus: Chemically Induced
Monarch Initiative (Disease and Phenotype Associations): MGI:96677
Gene Symbol: Kmt2c (Mus musculus (mouse))
Name: lysine (K)-specific methyltransferase 2C
Synonyms: HALR, E330008K23Rik, Mll3
Chromosome: 5
Alteration at locus: Chemically Induced
Monarch Initiative (Disease and Phenotype Associations): MGI:2444959
Gene Symbol: Espl1 (Mus musculus (mouse))
Name: extra spindle pole bodies 1, separase
Synonyms: SSE, separase, ESP1, PRCE, Cerp, PRCE
Chromosome: 15
Alteration at locus: Chemically Induced
Monarch Initiative (Disease and Phenotype Associations): MGI:2146156
Gene Symbol: Ncoa3 (Mus musculus (mouse))
Name: nuclear receptor coactivator 3
Synonyms: TRAM1, Src3, TRAM-1, AIB1, RAC3, pCIP, KAT13B, 2010305B15Rik, bHLHe42
Chromosome: 2
Alteration at locus: Chemically Induced
Monarch Initiative (Disease and Phenotype Associations): MGI:1276535
Gene Symbol: Gon4l (Mus musculus (mouse))
Name: gon-4-like (C.elegans)
Synonyms: 1500041I23Rik, 2610100B20Rik
Chromosome: 3
Alteration at locus: Chemically Induced
Monarch Initiative (Disease and Phenotype Associations): MGI:1917579
Gene Symbol: Rad54l2 (Mus musculus (mouse))
Name: RAD54 like 2 (S. cerevisiae)
Synonyms: Srisnf2l, Arip4, G630026H09Rik
Chromosome: 9
Alteration at locus: Chemically Induced
Monarch Initiative (Disease and Phenotype Associations): MGI:1933196
Gene Symbol: Rtel1 (Mus musculus (mouse))
Name: regulator of telomere elongation helicase 1
Chromosome: 2
Alteration at locus: Chemically Induced
Monarch Initiative (Disease and Phenotype Associations): MGI:2139369
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 138,067,925 bp
  • A to G, chromosome 1 at 155,232,450 bp
  • A to C, chromosome 2 at 148,732,264 bp
  • T to G, chromosome 2 at 166,054,497 bp
  • T to A, chromosome 2 at 181,355,796 bp
  • T to C, chromosome 3 at 51,448,365 bp
  • A to G, chromosome 3 at 87,867,407 bp
  • C to A, chromosome 3 at 88,907,387 bp
  • T to A, chromosome 3 at 107,756,739 bp
  • T to A, chromosome 4 at 45,941,877 bp
  • T to A, chromosome 4 at 74,128,298 bp
  • T to C, chromosome 4 at 113,236,518 bp
  • G to A, chromosome 4 at 136,051,143 bp
  • A to G, chromosome 4 at 155,890,580 bp
  • C to T, chromosome 5 at 25,315,326 bp
  • T to C, chromosome 5 at 72,296,975 bp
  • T to C, chromosome 5 at 75,640,499 bp
  • T to C, chromosome 5 at 87,775,821 bp
  • T to A, chromosome 5 at 110,337,205 bp
  • A to G, chromosome 5 at 114,452,961 bp
  • C to A, chromosome 6 at 21,950,064 bp
  • C to T, chromosome 6 at 29,493,203 bp
  • G to A, chromosome 6 at 47,798,392 bp
  • G to T, chromosome 6 at 122,071,007 bp
  • T to A, chromosome 6 at 137,380,266 bp
  • T to C, chromosome 6 at 140,579,465 bp
  • A to G, chromosome 7 at 6,317,681 bp
  • T to C, chromosome 7 at 103,470,071 bp
  • A to G, chromosome 7 at 127,328,281 bp
  • T to C, chromosome 8 at 3,954,635 bp
  • A to C, chromosome 8 at 61,533,372 bp
  • T to A, chromosome 9 at 39,719,807 bp
  • A to G, chromosome 9 at 106,693,222 bp
  • C to A, chromosome 10 at 62,315,540 bp
  • T to A, chromosome 10 at 111,269,419 bp
  • T to C, chromosome 11 at 100,012,427 bp
  • T to A, chromosome 12 at 4,770,182 bp
  • A to G, chromosome 13 at 49,494,431 bp
  • T to A, chromosome 13 at 49,805,200 bp
  • A to G, chromosome 13 at 60,719,684 bp
  • T to G, chromosome 14 at 16,409,189 bp
  • T to C, chromosome 14 at 25,636,010 bp
  • A to G, chromosome 14 at 33,369,510 bp
  • T to A, chromosome 14 at 49,770,436 bp
  • T to A, chromosome 14 at 54,962,108 bp
  • CT to CTTGGATT, chromosome 15 at 10,537,562 bp
  • G to A, chromosome 15 at 10,537,566 bp
  • A to G, chromosome 15 at 76,594,335 bp
  • A to G, chromosome 15 at 102,312,989 bp
  • G to A, chromosome 16 at 91,634,094 bp
  • T to C, chromosome 18 at 78,859,922 bp
  • C to T, chromosome 19 at 4,329,054 bp
  • T to C, chromosome 19 at 7,290,689 bp
  • C to A, chromosome X at 36,824,159 bp
Genotype Determination
ES Cell Line
N/A
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
MeSH Terms
    Strain Development
    C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
    Suggested Control Mice
    Wild-type littermates or C57BL/6J mice
    Primary Reference
    MUTAGENETIX record for R4377 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

    Colony and Husbandry Informationon

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
    Coat Color
    Black
    MMRRC Breeding System
    Random intra-strain mating
    Breeding Scheme(s)
    When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
    Overall Breeding Performance
    Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
    Average litter size
    Undetermined
    Recommended wean age
    3 weeks

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    The donor or their institution limits the distribution to non-profit institutions only.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
    041676-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.

    Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.