Strain Name:
C57BL/6J-MtgxR4386Btlr/Mmmh
Stock Number:
041680-MU
Citation ID:
RRID:MMRRC_041680-MU
Other Names:
R4386 (G1), C57BL/6J-MtgxR4386Btlr
Major Collection:

Gene Information

Cdc25a
Name: cell division cycle 25A
Synonyms: D9Ertd393e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 12530
HGNC: HGNC:1725
Homologene: 1355
March4
Name: membrane-associated ring finger (C3HC4) 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 381270
Homologene: 66199
Usp5
Name: ubiquitin specific peptidase 5 (isopeptidase T)
Synonyms: Ucht
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 22225
Homologene: 55758
Pik3c2a
Name: phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 alpha
Synonyms: PI3KC2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 18704
HGNC: HGNC:8971
Homologene: 20581
Psmd1
Name: proteasome (prosome, macropain) 26S subunit, non-ATPase, 1
Synonyms: P112, S1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 70247
HGNC: HGNC:9554
Homologene: 2100
Arid1b
Name: AT rich interactive domain 1B (SWI-like)
Synonyms: 9330189K18Rik, B230217J03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 239985
Homologene: 32344
Zfp369
Name: zinc finger protein 369
Synonyms: NRIF2, D230020H11Rik, B930030B22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 170936
Homologene: 129707
Lama1
Name: laminin, alpha 1
Synonyms: Lama
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 16772
VEGA: 17
HGNC: HGNC:6481
Homologene: 21146
Ncoa2
Name: nuclear receptor coactivator 2
Synonyms: SRC-2, TIF-2, D1Ertd433e, TIF2, Grip1, bHLHe75, glucocorticoid receptor-interacting protein 1, KAT13C, TIF2/GRIP-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 17978
HGNC: HGNC:7669
Homologene: 4768
Kirrel
Name: kirre like nephrin family adhesion molecule 1
Synonyms: Neph1, Kirrel1, 6720469N11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 170643
Homologene: 10089
Arap1
Name: ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1
Synonyms: Centd2, 2410002L19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 69710
Homologene: 12326
Cul2
Name: cullin 2
Synonyms: 4932411N15Rik, 1300003D18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 71745
HGNC: HGNC:2552
Homologene: 2662
Nsun6
Name: NOL1/NOP2/Sun domain family member 6
Synonyms: NOPD1, 4933414E04Rik, 4933403D21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 74455
Homologene: 12564
Nadk
Name: NAD kinase
Synonyms: 4432404C02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 192185
Homologene: 49724
Arhgap1
Name: Rho GTPase activating protein 1
Synonyms: p50rhoGAP, B230365D05Rik, Cdc42GAP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 228359
HGNC: HGNC:673
Homologene: 20909
Pkhd1
Name: polycystic kidney and hepatic disease 1
Synonyms: FPC, tigmin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 241035
HGNC: HGNC:9016
Homologene: 16336
Nuak1
Name: NUAK family, SNF1-like kinase, 1
Synonyms: B230104P22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 77976
Homologene: 8896
Kif1a
Name: kinesin family member 1A
Synonyms: C630002N23Rik, ATSV, Kns1, LOC381283, N-3 kinesin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 16560
HGNC: HGNC:888
Homologene: 99729
Skint5
Name: selection and upkeep of intraepithelial T cells 5
Synonyms: OTTMUSG00000008560
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 242627
Homologene: 135888
Ciz1
Name: CDKN1A interacting zinc finger protein 1
Synonyms: 2900056O04Rik, 0610038H21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 68379
Homologene: 8112
Olfr1395
Name: olfactory receptor 1395
Synonyms: GA_x6K02T2QP88-6286247-6285294, MOR277-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 258877
Homologene: 105890
Tnfaip3
Name: tumor necrosis factor, alpha-induced protein 3
Synonyms: A20, zinc finger protein A20, Tnfip3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 21929
Homologene: 4582
Rev3l
Name: REV3 like, DNA directed polymerase zeta catalytic subunit
Synonyms: Rev, Sez4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 19714
HGNC: HGNC:9968
Homologene: 48147
Olfr510
Name: olfactory receptor 510
Synonyms: GA_x6K02T2PBJ9-10997715-10998659, MOR204-34
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 258308
Cps1
Name: carbamoyl-phosphate synthetase 1
Synonyms: 4732433M03Rik, CPS, D1Ucla3, CPSase I
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 227231
HGNC: HGNC:2323
Homologene: 68208
Olfr291
Name: olfactory receptor 291
Synonyms: MOR254-2, GA_x6K02T2NHDJ-11231385-11230438
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 258410
Homologene: 83130
Sdk1
Name: sidekick cell adhesion molecule 1
Synonyms: 6720466O15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 330222
Homologene: 27395
Pabpc2
Name: poly(A) binding protein, cytoplasmic 2
Synonyms: Pabp2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 18459
Homologene: 56509
Spock1
Name: sparc/osteonectin, cwcv and kazal-like domains proteoglycan 1
Synonyms: testican 1, Ticn1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 20745
Homologene: 80193
Scgb1b2
Name: secretoglobin, family 1B, member 2
Synonyms: Mja1l, LGP, Scgb1b1, Apbh, Abph, Abpa2, lacrimal gland protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 57426
Homologene: 114479
Slc24a3
Name: solute carrier family 24 (sodium/potassium/calcium exchanger), member 3
Synonyms: NCKX3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 94249
Homologene: 41390
Fah
Name: fumarylacetoacetate hydrolase
Synonyms: swst
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 14085
HGNC: HGNC:3579
Homologene: 110
Vmn2r112
Name: vomeronasal 2, receptor 112
Synonyms: EG628185
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 628185
Homologene: 86604
Usp45
Name: ubiquitin specific petidase 45
Synonyms: Gcap7, 3110003C05Rik, 4930550B20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 77593
Homologene: 17674
Cluap1
Name: clusterin associated protein 1
Synonyms: 2310030D15Rik, 2610111M03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 76779
Homologene: 14831
Oosp1
Name: oocyte secreted protein 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 170834
Homologene: 87189
Vmn1r35
Name: vomeronasal 1 receptor 35
Synonyms: V1rc12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 171185
Homologene: 110800
Fam129c
Name: family with sequence similarity 129, member C
Synonyms: Bcnp1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 100037278
Homologene: 45462
Kif12
Name: kinesin family member 12
Synonyms: N-9 kinesin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 16552
Homologene: 7796
Acsm3
Name: acyl-CoA synthetase medium-chain family member 3
Synonyms: Sa, Sah
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 20216
Homologene: 74559
Hbq1b
Name: hemoglobin, theta 1B
Synonyms: F830116E18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 544763
HGNC: HGNC:4833
Homologene: 3895
Wfdc9
Name: WAP four-disulfide core domain 9
Synonyms: OTTMUSG00000001088
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 629754
Homologene: 77391
Tmem128
Name: transmembrane protein 128
Synonyms: 2810021O14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 66309
Homologene: 11944
Tmem186
Name: transmembrane protein 186
Synonyms: 4432406C05Rik, 2810440M13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 66690
Homologene: 9129
Fam221a
Name: family with sequence similarity 221, member A
Synonyms: D330028D13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 231946
Homologene: 18214
Olfr157
Name: olfactory receptor 157
Synonyms: mOR37c, Olfr37c, MOR262-12, OR37C, GA_x6K02T2N78B-16110014-16110970
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 100040268
Homologene: 133619
Gm13784
Name: predicted gene 13784
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
Ighv6-5
Name: immunoglobulin heavy variable V6-5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 639510
Gm6158
Name: predicted gene 6158
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 620499
VEGA: 14
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 13,177,165 bp
  • A to G, chromosome 1 at 20,414,292 bp
  • T to C, chromosome 1 at 67,170,995 bp
  • G to A, chromosome 1 at 72,428,814 bp
  • T to A, chromosome 1 at 86,128,192 bp
  • T to A, chromosome 1 at 93,068,550 bp
  • T to A, chromosome 2 at 14,996,522 bp
  • C to T, chromosome 2 at 32,370,099 bp
  • T to C, chromosome 2 at 91,668,237 bp
  • A to G, chromosome 2 at 145,606,826 bp
  • A to T, chromosome 2 at 164,650,538 bp
  • C to T, chromosome 3 at 87,089,151 bp
  • T to C, chromosome 4 at 21,830,505 bp
  • C to T, chromosome 4 at 43,836,124 bp
  • C to A, chromosome 4 at 63,171,208 bp
  • G to A, chromosome 4 at 63,171,218 bp
  • T to C, chromosome 4 at 113,483,893 bp
  • A to T, chromosome 4 at 155,582,575 bp
  • T to C, chromosome 5 at 38,262,074 bp
  • A to T, chromosome 5 at 114,241,921 bp
  • T to C, chromosome 5 at 142,094,626 bp
  • G to A, chromosome 6 at 49,378,432 bp
  • A to T, chromosome 6 at 66,679,589 bp
  • C to T, chromosome 6 at 124,818,474 bp
  • T to A, chromosome 7 at 31,290,664 bp
  • T to A, chromosome 7 at 84,599,136 bp
  • T to C, chromosome 7 at 84,856,548 bp
  • T to A, chromosome 7 at 101,385,571 bp
  • T to A, chromosome 7 at 108,668,253 bp
  • A to G, chromosome 7 at 116,354,099 bp
  • G to T, chromosome 7 at 119,773,871 bp
  • A to G, chromosome 8 at 71,607,511 bp
  • A to G, chromosome 9 at 109,889,733 bp
  • A to G, chromosome 10 at 19,007,010 bp
  • A to G, chromosome 10 at 39,836,681 bp
  • T to A, chromosome 10 at 84,394,044 bp
  • T to A, chromosome 11 at 32,287,295 bp
  • T to A, chromosome 11 at 49,149,015 bp
  • G to A, chromosome 12 at 114,416,717 bp
  • A to G, chromosome 13 at 57,440,450 bp
  • A to G, chromosome 13 at 65,296,992 bp
  • G to T, chromosome 14 at 24,070,294 bp
  • A to C, chromosome 16 at 3,933,722 bp
  • G to A, chromosome 16 at 8,636,023 bp
  • A to G, chromosome 17 at 4,994,972 bp
  • T to A, chromosome 17 at 22,601,322 bp
  • A to G, chromosome 17 at 67,773,712 bp
  • T to C, chromosome 18 at 3,434,856 bp
  • T to C, chromosome 18 at 39,775,185 bp
  • C to T, chromosome 19 at 11,667,794 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4386 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041680-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.