Strain Name:
C57BL/6J-MtgxR4397Btlr/Mmmh
Stock Number:
041685-MU
Citation ID:
RRID:MMRRC_041685-MU
Other Names:
R4397 (G1), C57BL/6J-MtgxR4397Btlr
Major Collection:

Gene Information

Gfap
Name: glial fibrillary acidic protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 14580
HGNC: HGNC:4235
Homologene: 1554
Nup188
Name: nucleoporin 188
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 227699
Homologene: 45683
Nphs1
Name: nephrosis 1, nephrin
Synonyms: nephrin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 54631
HGNC: HGNC:7908
Homologene: 20974
Baz1b
Name: bromodomain adjacent to zinc finger domain, 1B
Synonyms: Wbscr9, WSTF
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 22385
HGNC: HGNC:961
Homologene: 22651
Suco
Name: SUN domain containing ossification factor
Synonyms: osteopotentia, Opt, AI848100
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 226551
HGNC: HGNC:1240
Homologene: 32212
Virma
Name: vir like m6A methyltransferase associated
Synonyms: 1110037F02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 66185
Homologene: 41043
Dnajc15
Name: DnaJ heat shock protein family (Hsp40) member C15
Synonyms: 1110003P16Rik, Dnajd1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 66148
VEGA: 14
Homologene: 22740
Nup133
Name: nucleoporin 133
Synonyms: 4832420O05Rik, mermaid
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234865
Homologene: 32402
Ptpn21
Name: protein tyrosine phosphatase, non-receptor type 21
Synonyms: PTPD1, PTPRL10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 24000
VEGA: 12
HGNC: HGNC:9651
Homologene: 5110
Prpf8
Name: pre-mRNA processing factor 8
Synonyms: DBF3/PRP8, Prp8, D11Bwg0410e, Sfprp8l
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 192159
Homologene: 4706
Map3k12
Name: mitogen-activated protein kinase kinase kinase 12
Synonyms: Zpk, DLK, MUK
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 26404
HGNC: HGNC:6851
Homologene: 4592
Slit2
Name: slit guidance ligand 2
Synonyms: Slil3, Drad-1, E130320P19Rik, E030015M03Rik, b2b1200.1Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 20563
Homologene: 3516
Iqgap3
Name: IQ motif containing GTPase activating protein 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 404710
Homologene: 26400
Bmp1
Name: bone morphogenetic protein 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 12153
VEGA: 14
HGNC: HGNC:1067
Homologene: 55955
Kremen1
Name: kringle containing transmembrane protein 1
Synonyms: Krm1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 84035
Homologene: 12935
Hjurp
Name: Holliday junction recognition protein
Synonyms: C330011F01Rik, A730008H23Rik, 6430706D22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 381280
Homologene: 10184
H13
Name: histocompatibility 13
Synonyms: H-13, 1200006O09Rik, 5031424B04Rik, 4930443L17Rik, Spp, Hm13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 14950
Homologene: 7749
Rnf7
Name: ring finger protein 7
Synonyms: Rbx2, Sag
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 19823
Homologene: 84476
Homer3
Name: homer scaffolding protein 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 26558
Homologene: 37972
Magi3
Name: membrane associated guanylate kinase, WW and PDZ domain containing 3
Synonyms: 4732496O19Rik, 6530407C02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 99470
Homologene: 26431
Plxna2
Name: plexin A2
Synonyms: OCT, Plxn2, 2810428A13Rik, PlexA2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 18845
HGNC: HGNC:9100
Homologene: 56427
Klb
Name: klotho beta
Synonyms: betaKlotho
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 83379
Homologene: 12820
Prss38
Name: protease, serine 38
Synonyms: LOC216797, Gm249
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 216797
Homologene: 18831
Lamc3
Name: laminin gamma 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 23928
HGNC: HGNC:6494
Homologene: 21222
Fam135b
Name: family with sequence similarity 135, member B
Synonyms: A830008O07Rik, 1700010C24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 70363
VEGA: 15
Homologene: 66605
Nlrp1a
Name: NLR family, pyrin domain containing 1A
Synonyms: Nalp1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 195046
Homologene: 133820
Naip6
Name: NLR family, apoptosis inhibitory protein 6
Synonyms: Naip-rs4A, Naip-rs4, Birc1f
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 17952
HGNC: HGNC:7634
Homologene: 113589
Lrp4
Name: low density lipoprotein receptor-related protein 4
Synonyms: 6430526J12Rik, Megf7, mdig
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 228357
HGNC: HGNC:6696
Homologene: 17964
Plcb3
Name: phospholipase C, beta 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 18797
VEGA: 19
HGNC: HGNC:9056
Homologene: 47960
Rpgrip1
Name: retinitis pigmentosa GTPase regulator interacting protein 1
Synonyms: 4930505G06Rik, A930002K18Rik, 4930401L23Rik, nmf247
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 77945
Homologene: 10679
Tnxb
Name: tenascin XB
Synonyms: Tnx, TN-MHC
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 81877
Homologene: 49589
Vmn2r88
Name: vomeronasal 2, receptor 88
Synonyms: V2r13, V2r3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 669149
Homologene: 129606
Gm9581
Name: predicted gene 9581
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Agap2
Name: ArfGAP with GTPase domain, ankyrin repeat and PH domain 2
Synonyms: Centg1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 216439
VEGA: 10
Homologene: 86815
Vmn2r79
Name: vomeronasal 2, receptor 79
Synonyms: EG621430
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 621430
Homologene: 115466
Hcls1
Name: hematopoietic cell specific Lyn substrate 1
Synonyms: HS1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 15163
HGNC: HGNC:4844
Homologene: 38034
Mfsd2b
Name: major facilitator superfamily domain containing 2B
Synonyms: Gm1964
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 432628
Homologene: 47753
Iqsec2
Name: IQ motif and Sec7 domain 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 245666
Homologene: 19407
Mex3b
Name: mex3 RNA binding family member B
Synonyms: 4931439A04Rik, Rkhd3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 108797
Homologene: 13570
Psg16
Name: pregnancy specific glycoprotein 16
Synonyms: bCEA, Cea11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 26436
Slc25a11
Name: solute carrier family 25 (mitochondrial carrier oxoglutarate carrier), member 11
Synonyms: 2310022P18Rik, 2oxoc
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 67863
Homologene: 2637
Nars2
Name: asparaginyl-tRNA synthetase 2 (mitochondrial)(putative)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 244141
Homologene: 57002
Fancg
Name: Fanconi anemia, complementation group G
Synonyms: Xrcc9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 60534
HGNC: HGNC:3588
Homologene: 3402
Aig1
Name: androgen-induced 1
Synonyms: CGI-103, 1500031O19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 66253
Homologene: 32297
Olfr770
Name: olfactory receptor 770
Synonyms: GA_x6K02T2PULF-10819232-10818297, MOR114-5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 258862
Homologene: 27285
Trpv6
Name: transient receptor potential cation channel, subfamily V, member 6
Synonyms: CaT1, Cac, CAT, Ecac2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 64177
Homologene: 56812
Gjd3
Name: gap junction protein, delta 3
Synonyms: Gja11, connexin-30.2, cx30.2, connexin 30.2, Gjc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 353155
Homologene: 17530
Phactr3
Name: phosphatase and actin regulator 3
Synonyms: scapinin, 1500003N10Rik, 4930415A02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 74189
Homologene: 14482
Adgrg4
Name: adhesion G protein-coupled receptor G4
Synonyms: LOC236798, PGR17, Gpr112
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 236798
Homologene: 72131
Gvin-ps3
Name: GTPase, very large interferon inducible, pseudogene 3
Synonyms: Gm8979, Gm21884
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 668108
Gm20528
Name: predicted gene 20528
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
A830036E02Rik
Name: RIKEN cDNA A830036E02 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Pcdhga9
Name: protocadherin gamma subfamily A, 9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 93717
HGNC: HGNC:8707
Homologene: 56827
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • CAGAGAGAGAGAGA to CAGAGAGAGAGA, chromosome 1 at 88,211,984 bp
  • GT to GTT, chromosome 1 at 88,266,524 bp
  • A to G, chromosome 1 at 161,844,852 bp
  • C to T, chromosome 1 at 194,749,317 bp
  • C to T, chromosome 2 at 30,343,497 bp
  • A to G, chromosome 2 at 31,931,952 bp
  • T to C, chromosome 2 at 91,511,670 bp
  • A to G, chromosome 2 at 152,677,552 bp
  • A to G, chromosome 2 at 178,175,406 bp
  • T to C, chromosome 3 at 88,104,358 bp
  • T to C, chromosome 3 at 104,219,714 bp
  • A to G, chromosome 4 at 11,513,901 bp
  • A to T, chromosome 4 at 43,008,897 bp
  • G to T, chromosome 5 at 48,220,081 bp
  • A to G, chromosome 5 at 65,380,039 bp
  • C to T, chromosome 5 at 135,244,446 bp
  • A to G, chromosome 6 at 41,625,238 bp
  • T to C, chromosome 7 at 17,090,698 bp
  • T to G, chromosome 7 at 30,481,965 bp
  • T to C, chromosome 7 at 82,869,823 bp
  • A to G, chromosome 7 at 87,001,891 bp
  • T to C, chromosome 7 at 96,973,564 bp
  • A to G, chromosome 7 at 106,082,923 bp
  • G to A, chromosome 8 at 70,290,143 bp
  • G to A, chromosome 8 at 123,944,301 bp
  • A to G, chromosome 9 at 96,478,410 bp
  • A to C, chromosome 10 at 13,652,982 bp
  • G to A, chromosome 10 at 127,090,483 bp
  • G to T, chromosome 10 at 129,133,581 bp
  • A to T, chromosome 11 at 5,199,610 bp
  • T to C, chromosome 11 at 59,373,028 bp
  • G to A, chromosome 11 at 70,644,851 bp
  • T to G, chromosome 11 at 71,097,204 bp
  • T to C, chromosome 11 at 75,492,523 bp
  • A to T, chromosome 11 at 98,982,421 bp
  • A to T, chromosome 11 at 99,422,082 bp
  • G to A, chromosome 11 at 102,896,984 bp
  • C to G, chromosome 12 at 4,866,206 bp
  • G to T, chromosome 12 at 98,688,248 bp
  • A to G, chromosome 12 at 98,715,060 bp
  • C to T, chromosome 13 at 100,300,600 bp
  • A to T, chromosome 14 at 51,417,978 bp
  • T to C, chromosome 14 at 52,136,670 bp
  • C to T, chromosome 14 at 70,490,542 bp
  • T to C, chromosome 14 at 77,874,794 bp
  • T to A, chromosome 15 at 71,448,676 bp
  • C to T, chromosome 15 at 102,501,259 bp
  • T to C, chromosome 16 at 36,937,300 bp
  • A to G, chromosome 16 at 59,560,095 bp
  • C to T, chromosome 17 at 34,678,662 bp
  • A to T, chromosome 18 at 37,738,641 bp
  • T to C, chromosome 19 at 6,965,825 bp
  • T to G, chromosome X at 56,932,343 bp
  • C to T, chromosome X at 152,209,053 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4397 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041685-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.