Strain Name:
C57BL/6J-MtgxR4429Btlr/Mmmh
Stock Number:
041699-MU
Citation ID:
RRID:MMRRC_041699-MU
Other Names:
R4429 (G1), C57BL/6J-MtgxR4429Btlr
Major Collection:

Gene Information

Chrna4
Name: cholinergic receptor, nicotinic, alpha polypeptide 4
Synonyms: alpha4 nAChR, Acra4, Acra-4, a4 nicotinic receptor, alpha4-nAChR
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 11438
HGNC: HGNC:1958
Homologene: 592
Pcdh9
Name: protocadherin 9
Synonyms: C630029H24Rik, 1500001L12Rik, LOC382930, A730003J17Rik, C530050I23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 211712
HGNC: HGNC:8661
Homologene: 10698
Ahcyl2
Name: S-adenosylhomocysteine hydrolase-like 2
Synonyms: 4631427C17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 74340
Homologene: 69337
Hap1
Name: huntingtin-associated protein 1
Synonyms: HAP-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 15114
HGNC: HGNC:4812
Homologene: 2935
Stk11ip
Name: serine/threonine kinase 11 interacting protein
Synonyms: LKB1IP, LIP1, 1200014D22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 71728
Homologene: 12406
Sf3b3
Name: splicing factor 3b, subunit 3
Synonyms: 5730409A01Rik, 1810061H24Rik, SAP130, RSE1, D8Ertd633e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 101943
Homologene: 6579
Nol9
Name: nucleolar protein 9
Synonyms: 6030462G04Rik, 4632412I24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 74035
Homologene: 32589
Pparg
Name: peroxisome proliferator activated receptor gamma
Synonyms: Nr1c3, PPAR-gamma, Ppar-gamma2, PPARgamma, PPARgamma2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 19016
HGNC: HGNC:9236
Homologene: 7899
Slc12a3
Name: solute carrier family 12, member 3
Synonyms: NCC, TSC
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 20497
Homologene: 287
Uba6
Name: ubiquitin-like modifier activating enzyme 6
Synonyms: 5730469D23Rik, Ube1l2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231380
Homologene: 10080
Cnpy3
Name: canopy FGF signaling regulator 3
Synonyms: 2410050O22Rik, ERDA5, CAG4A, 1600025D17Rik, Tnrc5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 72029
Homologene: 4803
Nsd2
Name: nuclear receptor binding SET domain protein 2
Synonyms: 5830445G22Rik, 9430010A17Rik, Whsc1l, D030027O06Rik, C130020C13Rik, D930023B08Rik, Whsc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 107823
Homologene: 26175
Map3k14
Name: mitogen-activated protein kinase kinase kinase 14
Synonyms: Nik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 53859
HGNC: HGNC:6853
Homologene: 2940
Cldn8
Name: claudin 8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 54420
HGNC: HGNC:2050
Homologene: 8117
Dnah8
Name: dynein, axonemal, heavy chain 8
Synonyms: Hst6.7b, P1-Loop, Dnahc8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 13417
VEGA: 17
HGNC: HGNC:2952
Homologene: 1049
Sptbn2
Name: spectrin beta, non-erythrocytic 2
Synonyms: Spnb3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 20743
VEGA: 19
Homologene: 48482
Gtf2e2
Name: general transcription factor II E, polypeptide 2 (beta subunit)
Synonyms: C330006J08Rik, 34kDa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 68153
HGNC: HGNC:4651
Homologene: 37573
Lemd3
Name: LEM domain containing 3
Synonyms: Man1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 380664
Homologene: 8633
Siglecg
Name: sialic acid binding Ig-like lectin G
Synonyms: mSiglec-G, A630096C01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 243958
Homologene: 13228
Trhde
Name: TRH-degrading enzyme
Synonyms: 9330155P21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 237553
Homologene: 75007
Scn1a
Name: sodium channel, voltage-gated, type I, alpha
Synonyms: Nav1.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 20265
Homologene: 21375
Myo7a
Name: myosin VIIA
Synonyms: Myo7, USH1B, nmf371, polka, Hdb
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 17921
HGNC: HGNC:7606
Homologene: 219
Ano4
Name: anoctamin 4
Synonyms: A330096O15Rik, Tmem16d
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 320091
Homologene: 66599
Pclo
Name: piccolo (presynaptic cytomatrix protein)
Synonyms: Acz, Pico
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 26875
Homologene: 69111
Ephb3
Name: Eph receptor B3
Synonyms: Sek4, MDK5, HEK2, Etk2, Tyro6, Cek10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 13845
HGNC: HGNC:3394
Homologene: 20938
Iqcg
Name: IQ motif containing G
Synonyms: G1-374-12, stubby12d, repro1, 2400003L07Rik, esgd12d
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 69707
Homologene: 32776
Zfp867
Name: zinc finger protein 867
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 237775
Homologene: 64827
Nox3
Name: NADPH oxidase 3
Synonyms: het, nmf250
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 224480
HGNC: HGNC:7890
Homologene: 49435
Timm29
Name: translocase of inner mitochondrial membrane 29
Synonyms: 1810026J23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 69773
Homologene: 34702
Rhbdf1
Name: rhomboid 5 homolog 1
Synonyms: Dist1, Egfr-rs, Dist
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 13650
Homologene: 32085
Abca5
Name: ATP-binding cassette, sub-family A (ABC1), member 5
Synonyms: ABC13, B930033A02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 217265
HGNC: HGNC:35
Homologene: 10263
Myo5c
Name: myosin VC
Synonyms: 9130003O20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 208943
VEGA: 9
HGNC: HGNC:7604
Homologene: 135711
Zfp661
Name: zinc finger protein 661
Synonyms: 2810405K07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 72180
Homologene: 23275
Catip
Name: ciliogenesis associated TTC17 interacting protein
Synonyms: LOC241112, Gm216
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 241112
Serpina5
Name: serine (or cysteine) peptidase inhibitor, clade A, member 5
Synonyms: Pci, antitrypsin, alpha-1 antiproteinase, PAI-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 268591
HGNC: HGNC:8723
Homologene: 20159
0610040J01Rik
Name: RIKEN cDNA 0610040J01 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 76261
Homologene: 49537
Tmem67
Name: transmembrane protein 67
Synonyms: 5330408M12Rik, b2b1291.1Clo, b2b1163.1Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 329795
Homologene: 71886
Meioc
Name: meiosis specific with coiled-coil domain
Synonyms: LOC380729, LOC268491, Gm1564
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 268491
Homologene: 19559
Spdl1
Name: spindle apparatus coiled-coil protein 1
Synonyms: 1700018I02Rik, 2810049B11Rik, 2600001J17Rik, Ccdc99
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 70385
Homologene: 9837
Prag1
Name: PEAK1 related kinase activating pseudokinase 1
Synonyms: NACK, D8Ertd82e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 244418
Homologene: 18256
Slco6d1
Name: solute carrier organic anion transporter family, member 6d1
Synonyms: 4921511I05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 70866
Homologene: 86643
St8sia4
Name: ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 4
Synonyms: ST8SiaIV, PST, PST-1, Siat8d
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 20452
Homologene: 4147
Rita1
Name: RBPJ interacting and tubulin associated 1
Synonyms: 1110008J03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 100764
Homologene: 12585
Rsph6a
Name: radial spoke head 6 homolog A (Chlamydomonas)
Synonyms: RSP4, Rshl1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 83434
Homologene: 36476
Bag4
Name: BCL2-associated athanogene 4
Synonyms: 2410112I15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 67384
HGNC: HGNC:940
Homologene: 31270
Zp3r
Name: zona pellucida 3 receptor
Synonyms: SP56
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 22789
Homologene: 7609
Vmn1r174
Name: vomeronasal 1 receptor 174
Synonyms: V1rd22
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 404291
Homologene: 79577
Hacd4
Name: 3-hydroxyacyl-CoA dehydratase 4
Synonyms: 4933428I03Rik, Ptplad2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 66775
Homologene: 12033
Sytl5
Name: synaptotagmin-like 5
Synonyms: ENSMUSG00000054453
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 236643
Homologene: 16345
Mrps10
Name: mitochondrial ribosomal protein S10
Synonyms: 1110038B19Rik, Rpms10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 64657
Homologene: 10028
A730013G03Rik
Name: RIKEN cDNA A730013G03 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 320952
Lect2
Name: leukocyte cell-derived chemotaxin 2
Synonyms: chondromodulin-II
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 16841
VEGA: 13
HGNC: HGNC:6550
Homologene: 1730
Tmem181a
Name: transmembrane protein 181A
Synonyms: 5930418K15Rik, Gpr178, C76977, Tmem181
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 77106
Homologene: 44787
Havcr2
Name: hepatitis A virus cellular receptor 2
Synonyms: Tim3, Timd3, TIM-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 171285
Homologene: 129541
Lrrc72
Name: leucine rich repeat containing 72
Synonyms: 4933421E18Rik, 1700108M19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 71156
Homologene: 90358
Gm27047
Name: predicted gene, 27047
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Gm6594
Name: predicted pseudogene 6594
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 625514
VEGA: 17
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 74,368,732 bp
  • A to G, chromosome 1 at 75,528,892 bp
  • A to T, chromosome 1 at 95,660,849 bp
  • T to C, chromosome 1 at 98,496,366 bp
  • T to C, chromosome 1 at 130,591,391 bp
  • G to C, chromosome 1 at 192,833,740 bp
  • A to T, chromosome 2 at 66,350,985 bp
  • A to G, chromosome 2 at 127,578,708 bp
  • A to G, chromosome 2 at 181,028,620 bp
  • T to A, chromosome 4 at 12,051,473 bp
  • A to T, chromosome 4 at 88,434,947 bp
  • C to T, chromosome 4 at 152,041,174 bp
  • T to G, chromosome 5 at 14,678,100 bp
  • T to G, chromosome 5 at 33,843,202 bp
  • G to T, chromosome 5 at 63,898,839 bp
  • C to T, chromosome 5 at 86,120,547 bp
  • A to G, chromosome 5 at 120,609,561 bp
  • T to G, chromosome 6 at 29,894,875 bp
  • T to A, chromosome 6 at 115,440,023 bp
  • T to A, chromosome 6 at 130,631,011 bp
  • T to A, chromosome 7 at 19,074,063 bp
  • T to C, chromosome 7 at 23,754,140 bp
  • C to T, chromosome 7 at 43,417,926 bp
  • T to C, chromosome 7 at 98,053,188 bp
  • C to T, chromosome 8 at 25,769,488 bp
  • T to A, chromosome 8 at 33,752,493 bp
  • A to G, chromosome 8 at 36,146,642 bp
  • A to T, chromosome 8 at 94,343,085 bp
  • A to C, chromosome 8 at 110,826,118 bp
  • G to C, chromosome 9 at 21,593,479 bp
  • A to G, chromosome 9 at 75,294,001 bp
  • T to A, chromosome 10 at 88,992,942 bp
  • A to T, chromosome 10 at 114,503,123 bp
  • T to C, chromosome 10 at 120,977,988 bp
  • C to T, chromosome 11 at 32,213,369 bp
  • C to T, chromosome 11 at 34,822,563 bp
  • T to A, chromosome 11 at 46,456,560 bp
  • C to T, chromosome 11 at 59,465,037 bp
  • T to C, chromosome 11 at 100,354,272 bp
  • T to C, chromosome 11 at 102,675,720 bp
  • A to G, chromosome 11 at 103,227,584 bp
  • T to C, chromosome 11 at 110,311,410 bp
  • T to C, chromosome 12 at 36,208,624 bp
  • C to A, chromosome 12 at 104,103,406 bp
  • C to T, chromosome 13 at 56,545,725 bp
  • T to C, chromosome 14 at 93,887,384 bp
  • G to A, chromosome 16 at 21,214,463 bp
  • G to A, chromosome 16 at 33,019,490 bp
  • A to C, chromosome 16 at 88,562,731 bp
  • G to A, chromosome 17 at 3,682,958 bp
  • T to A, chromosome 17 at 6,295,786 bp
  • A to G, chromosome 17 at 30,752,146 bp
  • T to A, chromosome 17 at 46,747,144 bp
  • T to A, chromosome 17 at 47,378,199 bp
  • T to A, chromosome 17 at 82,539,494 bp
  • T to C, chromosome 19 at 4,738,355 bp
  • A to T, chromosome X at 9,960,023 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4429 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041699-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.