Strain Name:
C57BL/6J-MtgxR4446Btlr/Mmmh
Stock Number:
041707-MU
Citation ID:
RRID:MMRRC_041707-MU
Other Names:
R4446 (G1), C57BL/6J-MtgxR4446Btlr
Major Collection:

Strain Information

Mtmr3
Name: myotubularin related protein 3
Synonyms: 1700092A20Rik, ZFYVE10, FYVE-DSP1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 74302
HGNC: HGNC:7451
Homologene: 23662
Sptbn1
Name: spectrin beta, non-erythrocytic 1
Synonyms: Spnb2, elf1, elf3, spectrin G, brain spectrin, Spnb-2, 9930031C03Rik, non-erythrocytic, beta fodrin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 20742
Homologene: 2354
Sema6d
Name: sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D
Synonyms: 1110067B02Rik, Sema6D-6, Sema6D-4, Sema6D-1, Sema6D-2, Sema6D-5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 214968
Homologene: 16195
Dysf
Name: dysferlin
Synonyms: 2310004N10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 26903
HGNC: HGNC:3097
Homologene: 20748
Dgkh
Name: diacylglycerol kinase, eta
Synonyms: 5930402B05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 380921
VEGA: 14
HGNC: HGNC:2854
Homologene: 99373
Asxl2
Name: ASXL transcriptional regulator 2
Synonyms: 4930556B16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 75302
Homologene: 10102
Cep131
Name: centrosomal protein 131
Synonyms: Azi1, AZ1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 12009
Homologene: 7638
Hmmr
Name: hyaluronan mediated motility receptor (RHAMM)
Synonyms: Rhamm, CD168
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 15366
HGNC: HGNC:5012
Homologene: 8271
Mms19
Name: MMS19 cytosolic iron-sulfur assembly component
Synonyms: Mms19, 2610042O15Rik, Mms19l, C86341
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 72199
Homologene: 41480
Slc25a25
Name: solute carrier family 25 (mitochondrial carrier, phosphate carrier), member 25
Synonyms: 1110030N17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 227731
Homologene: 62165
Arrdc3
Name: arrestin domain containing 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 105171
Homologene: 69318
Tmprss6
Name: transmembrane serine protease 6
Synonyms: 1300008A22Rik, matriptase-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 71753
VEGA: 15
Homologene: 12408
Crot
Name: carnitine O-octanoyltransferase
Synonyms: 1200003H03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 74114
HGNC: HGNC:2366
Homologene: 10899
C2cd3
Name: C2 calcium-dependent domain containing 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 277939
Homologene: 19524
Acadvl
Name: acyl-Coenzyme A dehydrogenase, very long chain
Synonyms: VLCAD
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 11370
HGNC: HGNC:92
Homologene: 5
Zdhhc6
Name: zinc finger, DHHC domain containing 6
Synonyms: 5930409M18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 66980
VEGA: 19
Homologene: 41487
Klf5
Name: Kruppel-like transcription factor 5
Synonyms: CKLF, 4930520J07Rik, IKLF, Bteb2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 12224
VEGA: 14
HGNC: HGNC:6349
Homologene: 37520
Rnf13
Name: ring finger protein 13
Synonyms: Rzf, 2010001H16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 24017
Homologene: 13493
Homer3
Name: homer scaffolding protein 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 26558
Homologene: 37972
Plxna2
Name: plexin A2
Synonyms: Plxn2, PlexA2, 2810428A13Rik, OCT
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 18845
HGNC: HGNC:9100
Homologene: 56427
Umod
Name: uromodulin
Synonyms: Urehd1, urehr4, Tamm-Horsfall glycoprotein, uromucoid
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 22242
Homologene: 2522
Cdkl2
Name: cyclin dependent kinase like 2
Synonyms: 5330436L21Rik, KKIAMRE, Kkm
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 53886
HGNC: HGNC:1782
Homologene: 55793
Tatdn2
Name: TatD DNase domain containing 2
Synonyms: mKIAA0218
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 381801
Homologene: 8848
Ttn
Name: titin
Synonyms: D330041I19Rik, 2310057K23Rik, connectin, shru, 1100001C23Rik, L56, mdm, D830007G01Rik, 2310074I15Rik, 2310036G12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 22138
Homologene: 130650
Myo3a
Name: myosin IIIA
Synonyms: 9030416P08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 667663
HGNC: HGNC:7601
Homologene: 49486
Fzd1
Name: frizzled class receptor 1
Synonyms: Fz1, FZ-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 14362
HGNC: HGNC:4038
Homologene: 20750
Dgkb
Name: diacylglycerol kinase, beta
Synonyms: C630029D13Rik, DGK-beta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 217480
VEGA: 12
HGNC: HGNC:2850
Homologene: 37875
Vmn2r93
Name: vomeronasal 2, receptor 93
Synonyms: EG627132
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 627132
Homologene: 129750
Or10n1
Name: olfactory receptor family 10 subfamily N member 1
Synonyms: M30, Olfr148, MOR224-4, GA_x6K02T2PVTD-33310486-33311418
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 258498
VEGA: 9
Homologene: 128136
Atp9a
Name: ATPase, class II, type 9A
Synonyms: Class II, IIa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 11981
Homologene: 69194
Igsf9b
Name: immunoglobulin superfamily, member 9B
Synonyms: LOC235086, AI414108
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235086
Homologene: 19472
Akt1
Name: thymoma viral proto-oncogene 1
Synonyms: PKB/Akt, Akt, PKBalpha, PKB
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 11651
HGNC: HGNC:391
Homologene: 3785
Cyp17a1
Name: cytochrome P450, family 17, subfamily a, polypeptide 1
Synonyms: p450c17, Cyp17, steroid 17-alpha hydroxylase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 13074
HGNC: HGNC:2593
Homologene: 73875
Adgrg6
Name: adhesion G protein-coupled receptor G6
Synonyms: 1190004A11Rik, DREG, LOC215798, Gpr126
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 215798
Homologene: 10724
Or3a1d
Name: olfactory receptor family 3 subfamily A member 1D
Synonyms: Olfr411, MOR255-3, GA_x6K02T2P1NL-4481525-4480578
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 258704
Homologene: 74999
Ssx2ip
Name: SSX family member 2 interacting protein
Synonyms: Adip
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 99167
Homologene: 8522
Or1ad6
Name: olfactory receptor family 1 subfamily AD member 6
Synonyms: GA_x6K02T2QP88-4469162-4468215, MOR129-2, Olfr1378
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 258912
Homologene: 133679
C4bp
Name: complement component 4 binding protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 12269
HGNC: HGNC:1325
A730013G03Rik
Name: RIKEN cDNA A730013G03 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 320952
Lrat
Name: lecithin-retinol acyltransferase (phosphatidylcholine-retinol-O-acyltransferase)
Synonyms: 1300010A18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 79235
HGNC: HGNC:6685
Homologene: 3483
Dipk1a
Name: divergent protein kinase domain 1A
Synonyms: 2900024C23Rik, Fam69a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 67266
Homologene: 12104
Pcdhgb6
Name: protocadherin gamma subfamily B, 6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 93703
Homologene: 49573
Gm20997
Name: predicted gene, 20997
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Pramel42
Name: PRAME like 42
Synonyms: Gm3139
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 100041102
Homologene: 133100
Pcdha6
Name: protocadherin alpha 6
Synonyms: Cnr2, Crnr2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 12937
Homologene: 75098
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 130,642,955 bp
  • T to A, chromosome 1 at 192,833,423 bp
  • C to T, chromosome 1 at 194,749,317 bp
  • A to T, chromosome 2 at 22,600,137 bp
  • T to C, chromosome 2 at 32,430,609 bp
  • A to G, chromosome 2 at 76,856,866 bp
  • A to G, chromosome 2 at 124,664,059 bp
  • C to T, chromosome 2 at 168,681,997 bp
  • A to C, chromosome 3 at 57,820,589 bp
  • A to G, chromosome 3 at 82,896,986 bp
  • G to A, chromosome 3 at 146,426,431 bp
  • A to G, chromosome 5 at 4,755,777 bp
  • T to C, chromosome 5 at 8,973,643 bp
  • G to A, chromosome 5 at 92,020,309 bp
  • T to A, chromosome 5 at 94,537,843 bp
  • T to C, chromosome 5 at 107,924,634 bp
  • A to G, chromosome 6 at 84,205,872 bp
  • T to G, chromosome 6 at 113,702,540 bp
  • C to A, chromosome 6 at 130,483,310 bp
  • C to T, chromosome 7 at 100,374,477 bp
  • A to G, chromosome 7 at 119,466,056 bp
  • G to A, chromosome 8 at 70,290,143 bp
  • A to G, chromosome 9 at 27,334,252 bp
  • A to T, chromosome 9 at 39,613,998 bp
  • A to G, chromosome 10 at 14,409,763 bp
  • G to A, chromosome 11 at 4,488,143 bp
  • C to T, chromosome 11 at 30,139,114 bp
  • T to C, chromosome 11 at 40,715,321 bp
  • C to T, chromosome 11 at 50,969,863 bp
  • C to A, chromosome 11 at 70,012,219 bp
  • T to C, chromosome 11 at 74,346,762 bp
  • T to C, chromosome 11 at 120,064,819 bp
  • T to A, chromosome 12 at 3,501,774 bp
  • G to T, chromosome 12 at 38,184,953 bp
  • C to T, chromosome 12 at 112,659,133 bp
  • T to C, chromosome 13 at 80,889,063 bp
  • A to G, chromosome 14 at 78,628,083 bp
  • C to T, chromosome 14 at 99,302,230 bp
  • A to G, chromosome 15 at 78,452,839 bp
  • T to A, chromosome 17 at 18,304,050 bp
  • A to G, chromosome 18 at 36,967,760 bp
  • A to G, chromosome 18 at 37,675,885 bp
  • A to T, chromosome 19 at 41,963,933 bp
  • A to G, chromosome 19 at 46,668,023 bp
  • T to C, chromosome 19 at 55,302,737 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4446 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041707-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.