Strain Name:
C57BL/6J-MtgxR4460Btlr/Mmmh
Stock Number:
041719-MU
Citation ID:
RRID:MMRRC_041719-MU
Other Names:
R4460 (G1), C57BL/6J-MtgxR4460Btlr
Major Collection:

Gene Information

Pou2f1
Name: POU domain, class 2, transcription factor 1
Synonyms: Oct-1A, Otf1, Otf-1, Oct1, oct-1, 2810482H01Rik, Oct-1C, Oct-1z, Oct-1B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 18986
HGNC: HGNC:9212
Homologene: 37658
Snx2
Name: sorting nexin 2
Synonyms: 0610030A03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 67804
VEGA: 18
Homologene: 2332
Ddx23
Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 23
Synonyms: 3110082M05Rik, 4921506D17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 74351
Homologene: 3542
Nol9
Name: nucleolar protein 9
Synonyms: 4632412I24Rik, 6030462G04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 74035
Homologene: 32589
Snx30
Name: sorting nexin family member 30
Synonyms: C030041J06Rik, 4732481H14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 209131
Homologene: 2811
Nav2
Name: neuron navigator 2
Synonyms: RAINB2, HELAD1, Unc53H2, 5330421F07Rik, POMFIL2, Rainb1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 78286
Homologene: 52330
Rasal2
Name: RAS protein activator like 2
Synonyms: A330066M24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 226525
HGNC: HGNC:9874
Homologene: 35217
Ctr9
Name: CTR9 homolog, Paf1/RNA polymerase II complex component
Synonyms: Tsbp, Tsp, Sh2bp1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 22083
Homologene: 40668
Celf6
Name: CUGBP, Elav-like family member 6
Synonyms: Brunol6, 6330569O16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 76183
Homologene: 129318
Ubr2
Name: ubiquitin protein ligase E3 component n-recognin 2
Synonyms: E130209G04Rik, ENSMUSG00000043296, 9930021A08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 224826
VEGA: 17
Homologene: 26151
Kirrel
Name: kirre like nephrin family adhesion molecule 1
Synonyms: Neph1, Kirrel1, 6720469N11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 170643
Homologene: 10089
Ptov1
Name: prostate tumor over expressed gene 1
Synonyms: 1110030G05Rik, Gcap3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 84113
HGNC: HGNC:9632
Homologene: 9687
Klhl26
Name: kelch-like 26
Synonyms: C630013N10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 234378
Homologene: 41247
Dnajc13
Name: DnaJ heat shock protein family (Hsp40) member C13
Synonyms: D030002L11Rik, LOC382100, Rme8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 235567
Homologene: 13574
A830010M20Rik
Name: RIKEN cDNA A830010M20 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 231570
VEGA: 5
Homologene: 128318
Itga2
Name: integrin alpha 2
Synonyms: DX5, CD49B, VLA-2 receptor, alpha 2 subunit
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 16398
VEGA: 13
HGNC: HGNC:6137
Homologene: 1662
Pla2g4e
Name: phospholipase A2, group IVE
Synonyms: 2310026J01Rik, Pla2epsilon
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 329502
Homologene: 65339
Ttn
Name: titin
Synonyms: shru, connectin, mdm, 2310057K23Rik, D330041I19Rik, 1100001C23Rik, D830007G01Rik, 2310036G12Rik, L56, 2310074I15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 22138
Homologene: 130650
Mmp9
Name: matrix metallopeptidase 9
Synonyms: Gel B, MMP-9, Clg4b, B/MMP9, Gelatinase B, gelatinase B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 17395
HGNC: HGNC:7176
Homologene: 3659
Mx1
Name: MX dynamin-like GTPase 1
Synonyms: Mx, myxovirus (influenza) resistance 1 polypeptide, Mx-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 17857
Nlrp9c
Name: NLR family, pyrin domain containing 9C
Synonyms: Nalp-zeta, Nalp9c
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 330490
Homologene: 116072
9530053A07Rik
Name: RIKEN cDNA 9530053A07 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 319482
Homologene: 130055
Dscam
Name: DS cell adhesion molecule
Synonyms: 4932410A21Rik, Down syndrome cell adhesion molecule
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 13508
HGNC: HGNC:3039
Homologene: 74393
Zfp804b
Name: zinc finger protein 804B
Synonyms: LOC207618
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 207618
Homologene: 52304
Ulk4
Name: unc-51-like kinase 4
Synonyms: 4932415A06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 209012
Homologene: 41205
Guf1
Name: GUF1 homolog, GTPase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 231279
Homologene: 6505
Cts6
Name: cathepsin 6
Synonyms: 1600022N02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 58518
Homologene: 75165
Mroh5
Name: maestro heat-like repeat family member 5
Synonyms: LOC268816, Gm628
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 268816
Cav1
Name: caveolin 1, caveolae protein
Synonyms: Cav-1, caveolin-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 12389
HGNC: HGNC:1527
Homologene: 1330
Tmem143
Name: transmembrane protein 143
Synonyms: 2310076O21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 70209
Homologene: 10105
Vmn2r121
Name: vomeronasal 2, receptor 121
Synonyms: EG625699
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
Alteration at locus: Chemically Induced
NCBI: 100038941
Homologene: 129606
Ipo4
Name: importin 4
Synonyms: 8430408O15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 75751
Homologene: 5835
Prkn
Name: parkin RBR E3 ubiquitin protein ligase
Synonyms: PRKN, Park2, Parkin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 50873
HGNC: HGNC:8607
Homologene: 3355
Rbbp8nl
Name: RBBP8 N-terminal like
Synonyms: BC066135
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 271887
Homologene: 76811
Vmn1r203
Name: vomeronasal 1 receptor 203
Synonyms: V1rh11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 171270
Homologene: 110880
Muc1
Name: mucin 1, transmembrane
Synonyms: Muc-1, CD227, EMA
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 17829
HGNC: HGNC:7508
Homologene: 137248
Itpka
Name: inositol 1,4,5-trisphosphate 3-kinase A
Synonyms: IP3-kinase A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 228550
HGNC: HGNC:6178
Homologene: 1671
C030048H21Rik
Name: RIKEN cDNA C030048H21 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
Rgmb
Name: repulsive guidance molecule family member B
Synonyms: RGM domain family, member B, DRAGON, 1110059F19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 68799
VEGA: 17
Homologene: 65355
Ptcd1
Name: pentatricopeptide repeat domain 1
Synonyms: 1110069M14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 71799
Homologene: 33161
Gm17690
Name: predicted gene, 17690
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
Gm20517
Name: predicted gene 20517
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 157,175,832 bp
  • A to G, chromosome 1 at 165,895,006 bp
  • A to G, chromosome 2 at 26,255,863 bp
  • A to C, chromosome 2 at 76,814,647 bp
  • A to G, chromosome 2 at 119,755,613 bp
  • T to G, chromosome 2 at 120,186,382 bp
  • G to T, chromosome 2 at 164,949,038 bp
  • A to G, chromosome 2 at 180,280,971 bp
  • C to T, chromosome 3 at 87,089,151 bp
  • A to T, chromosome 3 at 89,231,563 bp
  • T to C, chromosome 3 at 96,167,891 bp
  • A to T, chromosome 4 at 59,885,022 bp
  • T to G, chromosome 4 at 152,057,836 bp
  • A to T, chromosome 5 at 6,771,481 bp
  • C to T, chromosome 5 at 69,565,493 bp
  • A to G, chromosome 5 at 107,503,765 bp
  • G to T, chromosome 5 at 145,159,506 bp
  • A to G, chromosome 6 at 17,306,472 bp
  • T to A, chromosome 7 at 26,378,098 bp
  • C to T, chromosome 7 at 28,152,856 bp
  • T to C, chromosome 7 at 44,865,576 bp
  • C to T, chromosome 7 at 45,906,952 bp
  • T to C, chromosome 7 at 49,362,121 bp
  • A to G, chromosome 7 at 111,046,894 bp
  • T to C, chromosome 8 at 70,451,544 bp
  • G to A, chromosome 9 at 59,603,044 bp
  • C to A, chromosome 9 at 104,181,063 bp
  • A to T, chromosome 9 at 121,156,884 bp
  • T to A, chromosome 13 at 22,524,682 bp
  • G to C, chromosome 13 at 61,195,458 bp
  • C to G, chromosome 13 at 114,843,483 bp
  • G to A, chromosome 14 at 55,629,820 bp
  • A to T, chromosome 15 at 73,791,796 bp
  • A to C, chromosome 15 at 98,650,269 bp
  • A to G, chromosome 16 at 96,610,319 bp
  • G to T, chromosome 16 at 97,454,081 bp
  • A to T, chromosome 17 at 12,061,646 bp
  • C to A, chromosome 17 at 15,807,626 bp
  • C to T, chromosome 17 at 46,945,045 bp
  • T to C, chromosome 17 at 47,618,917 bp
  • A to G, chromosome 18 at 53,176,444 bp
  • G to A, chromosome X at 124,128,584 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4460 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041719-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.