Strain Name:
C57BL/6J-MtgxR4469Btlr/Mmmh
Stock Number:
041726-MU
Citation ID:
RRID:MMRRC_041726-MU
Other Names:
R4469 (G1), C57BL/6J-MtgxR4469Btlr
Major Collection:

Gene Information

Herc3
Name: hect domain and RLD 3
Synonyms: 5730409F18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 73998
HGNC: HGNC:4876
Homologene: 57095
Dst
Name: dystonin
Synonyms: bullous pemphigoid antigen 1, BPAG1-n, BPAG1, Bpag1, Bpag, ah, bullous pemphigoid antigen 1, athetoid, Macf2, 2310001O04Rik, nmf203, A830042E19Rik, nmf339
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 13518
HGNC: HGNC:1090
Homologene: 136716
Ubp1
Name: upstream binding protein 1
Synonyms: NF2d9, LBP-1b, LBP-1a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 22221
VEGA: 9
Homologene: 8435
Chd1
Name: chromodomain helicase DNA binding protein 1
Synonyms: 4930525N21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 12648
HGNC: HGNC:1915
Homologene: 68174
Kif11
Name: kinesin family member 11
Synonyms: Eg5, Knsl1, Kifl1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 16551
VEGA: 19
HGNC: HGNC:6388
Homologene: 3322
Ankrd11
Name: ankyrin repeat domain 11
Synonyms: 2410104C19Rik, 9530048I21Rik, 3010027A04Rik, Yod
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 77087
Homologene: 69134
Skp1
Name: S-phase kinase-associated protein 1
Synonyms: 2610043E24Rik, 2610206H23Rik, p19Skp1, Tceb1l, Skp1a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 21402
Homologene: 38775
Brpf1
Name: bromodomain and PHD finger containing, 1
Synonyms: 4930540D11Rik, 4833438B11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 78783
Homologene: 31251
4833420G17Rik
Name: RIKEN cDNA 4833420G17 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 67392
VEGA: 13
Homologene: 18889
Ackr1
Name: atypical chemokine receptor 1 (Duffy blood group)
Synonyms: CCBP1, FY, CD234, ESTM35, Dfy, Darc
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 13349
HGNC: HGNC:4035
Homologene: 48067
Gpsm1
Name: G-protein signalling modulator 1 (AGS3-like, C. elegans)
Synonyms: 1810037C22Rik, Ags3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 67839
Homologene: 16987
Sos1
Name: SOS Ras/Rac guanine nucleotide exchange factor 1
Synonyms: 4430401P03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 20662
VEGA: 17
Homologene: 4117
Dnah7a
Name: dynein, axonemal, heavy chain 7A
Synonyms: LOC381341, Dnahc7, Dnahc7a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 627872
Homologene: 41287
Asb4
Name: ankyrin repeat and SOCS box-containing 4
Synonyms: 8430401O13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 65255
Homologene: 9392
Snx29
Name: sorting nexin 29
Synonyms: LOC381035, LOC385605, 4933437K13Rik, Gm11170, Rundc2a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 74478
Homologene: 32706
Fbxl5
Name: F-box and leucine-rich repeat protein 5
Synonyms: Fir4, Fbl4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 242960
Homologene: 8129
Kirrel
Name: kirre like nephrin family adhesion molecule 1
Synonyms: Neph1, Kirrel1, 6720469N11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 170643
Homologene: 10089
Dnmt3c
Name: DNA methyltransferase 3C
Synonyms: Gm14490, Dnmt3c-ps
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 668932
Ier3ip1
Name: immediate early response 3 interacting protein 1
Synonyms: 1110057H19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 66191
Homologene: 41106
Ttn
Name: titin
Synonyms: connectin, L56, mdm, 1100001C23Rik, D830007G01Rik, 2310036G12Rik, 2310074I15Rik, 2310057K23Rik, D330041I19Rik, shru
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 22138
Homologene: 130650
Txndc11
Name: thioredoxin domain containing 11
Synonyms: EF-hand binding protein 1, EFP1, Txdc11, 2810408E11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 106200
Homologene: 9301
Arsi
Name: arylsulfatase i
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 545260
Homologene: 44428
Csf1
Name: colony stimulating factor 1 (macrophage)
Synonyms: CSF-1, M-CSF, colony-stimulating factor-1, Csfm
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 12977
HGNC: HGNC:2432
Homologene: 7282
Efcab7
Name: EF-hand calcium binding domain 7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230500
Homologene: 19952
Ces2g
Name: carboxylesterase 2G
Synonyms: 2210023G05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 72361
HGNC: HGNC:1864
Homologene: 77154
Cntnap4
Name: contactin associated protein-like 4
Synonyms: Caspr4, E130114F09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 170571
Homologene: 24912
Nlrc5
Name: NLR family, CARD domain containing 5
Synonyms: AI451557
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 434341
Homologene: 88935
Hcrtr2
Name: hypocretin (orexin) receptor 2
Synonyms: OX2r, mOX2bR, mOX2aR, mOXR2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 387285
HGNC: HGNC:4849
Homologene: 1168
Arhgef5
Name: Rho guanine nucleotide exchange factor (GEF) 5
Synonyms: 2210412D05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 54324
Homologene: 66300
2210016F16Rik
Name: RIKEN cDNA 2210016F16 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 70153
Homologene: 13005
Ror2
Name: receptor tyrosine kinase-like orphan receptor 2
Synonyms: Ntrkr2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 26564
Homologene: 55831
Trim75
Name: tripartite motif-containing 75
Synonyms: LOC333307
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 333307
Homologene: 53452
Ssbp2
Name: single-stranded DNA binding protein 2
Synonyms: Hspc116, 2310079I02Rik, 9330163K02Rik, 1500004K09Rik, A830008M03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 66970
Homologene: 22724
Zfp616
Name: zinc finger protein 616
Synonyms: Gm12330
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 327963
Homologene: 88945
Nostrin
Name: nitric oxide synthase trafficker
Synonyms: mDaIP2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 329416
Homologene: 43142
Lancl2
Name: LanC (bacterial lantibiotic synthetase component C)-like 2
Synonyms: 1700003F10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 71835
HGNC: HGNC:6509
Homologene: 23116
Stom
Name: stomatin
Synonyms: stomatin, protein 7.2b, Epb7.2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 13830
HGNC: HGNC:3383
Homologene: 81681
Rabggta
Name: Rab geranylgeranyl transferase, a subunit
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 56187
VEGA: 14
HGNC: HGNC:9795
Homologene: 32443
G6pd2
Name: glucose-6-phosphate dehydrogenase 2
Synonyms: Gpd2, Gpd-2, G6pdx-ps1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 14380
Adgra1
Name: adhesion G protein-coupled receptor A1
Synonyms: 2900059M17Rik, D7Ertd680e, Gpr123
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 52389
Homologene: 18582
Gtpbp2
Name: GTP binding protein 2
Synonyms: nmf205
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 56055
HGNC: HGNC:4670
Homologene: 10420
Gm9376
Name: predicted gene 9376
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 668814
Trav16d-dv11
Name: T cell receptor alpha variable 16D-DV11
Synonyms: ENSMUSG00000072535
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 547329
Olfr125
Name: olfactory receptor 125
Synonyms: MOR218-8, GA_x6K02T2PSCP-2291580-2292542
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 258287
Homologene: 114633
Smad9
Name: SMAD family member 9
Synonyms: Madh9, SMAD8B, SMAD8A, MADH6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 55994
HGNC: HGNC:6774
Homologene: 21198
Hsd17b1
Name: hydroxysteroid (17-beta) dehydrogenase 1
Synonyms: 17beta-HSD, Hsd17ba
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 15485
HGNC: HGNC:5210
Homologene: 37303
Unc5cl
Name: unc-5 family C-terminal like
Synonyms: 2510009H09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 76589
Homologene: 18319
Gm8055
Name: predicted pseudogene 8055
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 666342
Rps6-ps2
Name: ribosomal protein S6, pseudogene 2
Synonyms: Gm16406
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 675985
Olfr104-ps
Name: olfactory receptor 104, pseudogene
Synonyms: MOR250-5, GA_x6K02T2PSCP-1823235-1824161, Olfr104
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 257948
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 34,191,842 bp
  • T to A, chromosome 1 at 53,444,526 bp
  • T to C, chromosome 1 at 173,332,545 bp
  • G to A, chromosome 2 at 26,319,831 bp
  • T to C, chromosome 2 at 35,321,533 bp
  • C to G, chromosome 2 at 69,175,717 bp
  • T to C, chromosome 2 at 76,717,761 bp
  • A to G, chromosome 2 at 153,720,360 bp
  • T to C, chromosome 3 at 54,782,761 bp
  • C to T, chromosome 3 at 87,089,151 bp
  • C to T, chromosome 3 at 107,750,681 bp
  • A to G, chromosome 4 at 99,909,704 bp
  • A to G, chromosome 5 at 43,768,186 bp
  • A to T, chromosome 5 at 61,808,945 bp
  • C to A, chromosome 6 at 5,423,409 bp
  • T to C, chromosome 6 at 43,275,099 bp
  • T to A, chromosome 6 at 57,713,034 bp
  • T to A, chromosome 6 at 58,876,809 bp
  • T to C, chromosome 6 at 113,321,981 bp
  • C to T, chromosome 7 at 139,876,061 bp
  • A to G, chromosome 8 at 64,983,717 bp
  • A to G, chromosome 8 at 88,806,691 bp
  • A to T, chromosome 8 at 94,520,839 bp
  • A to T, chromosome 8 at 104,965,970 bp
  • A to T, chromosome 8 at 112,665,266 bp
  • A to G, chromosome 8 at 122,896,587 bp
  • T to A, chromosome 9 at 76,230,556 bp
  • C to T, chromosome 9 at 113,958,707 bp
  • T to C, chromosome 10 at 40,012,095 bp
  • C to T, chromosome 11 at 52,245,078 bp
  • T to A, chromosome 11 at 74,071,124 bp
  • A to T, chromosome 11 at 101,080,012 bp
  • G to A, chromosome 13 at 53,131,980 bp
  • A to G, chromosome 13 at 58,382,425 bp
  • G to A, chromosome 13 at 91,694,056 bp
  • A to G, chromosome 13 at 119,469,809 bp
  • A to G, chromosome 14 at 53,047,578 bp
  • A to G, chromosome 14 at 55,716,487 bp
  • C to T, chromosome 14 at 118,267,599 bp
  • T to C, chromosome 16 at 11,075,223 bp
  • T to A, chromosome 16 at 11,420,701 bp
  • T to C, chromosome 17 at 15,760,395 bp
  • A to G, chromosome 17 at 37,362,637 bp
  • T to C, chromosome 17 at 37,835,716 bp
  • A to G, chromosome 17 at 46,161,313 bp
  • C to G, chromosome 17 at 48,459,805 bp
  • A to G, chromosome 17 at 80,453,811 bp
  • G to A, chromosome 18 at 60,916,651 bp
  • A to G, chromosome 18 at 76,940,598 bp
  • A to G, chromosome 19 at 37,416,492 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
(Deceased) Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4469 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041726-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.