Strain Name:
C57BL/6J-MtgxR4489Btlr/Mmmh
Stock Number:
041745-MU
Citation ID:
RRID:MMRRC_041745-MU
Other Names:
R4489 (G1), C57BL/6J-MtgxR4489Btlr
Major Collection:

Gene Information

Ano1
Name: anoctamin 1, calcium activated chloride channel
Synonyms: Tmem16a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 101772
Homologene: 75079
Htr1b
Name: 5-hydroxytryptamine (serotonin) receptor 1B
Synonyms: 5-HT1B receptor, 5HT1B receptor, 5-HT1B receptor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 15551
HGNC: HGNC:5287
Homologene: 669
Kifbp
Name: kinesin family binding protein
Synonyms: 2510003E04Rik, Kif1bp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 72320
Homologene: 9223
Smchd1
Name: SMC hinge domain containing 1
Synonyms: 4931400A14Rik, MommeD1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 74355
Homologene: 23665
Nufip2
Name: nuclear fragile X mental retardation protein interacting protein 2
Synonyms: 9530056D24Rik, 1110001M19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 68564
Homologene: 10808
4932438A13Rik
Name: RIKEN cDNA 4932438A13 gene
Synonyms: Tweek, FSA
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229227
Homologene: 52105
Ank3
Name: ankyrin 3, epithelial
Synonyms: Ank-3, Ankyrin-3, AnkG, 2900054D09Rik, Ankyrin-G
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 11735
HGNC: HGNC:494
Homologene: 56908
Tg
Name: thyroglobulin
Synonyms: Tgn
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 21819
Homologene: 2430
Washc3
Name: WASH complex subunit 3
Synonyms: 5730495F03Rik, 2900091E11Rik, Ccdc53
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 67282
Homologene: 9364
Gigyf2
Name: GRB10 interacting GYF protein 2
Synonyms: A830080H02Rik, 2610016F01Rik, Tnrc15
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 227331
Homologene: 41048
Lzts1
Name: leucine zipper, putative tumor suppressor 1
Synonyms: F37, FEZ1, PSD-Zip70
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 211134
Homologene: 10873
Rnf13
Name: ring finger protein 13
Synonyms: Rzf, 2010001H16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 24017
Homologene: 13493
Homer3
Name: homer scaffolding protein 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 26558
Homologene: 37972
Cand2
Name: cullin-associated and neddylation-dissociated 2 (putative)
Synonyms: Tp120b, 2210404G23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 67088
Homologene: 41657
Mzt1
Name: mitotic spindle organizing protein 1
Synonyms: 2410129H14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 76789
VEGA: 14
Homologene: 45495
Uggt1
Name: UDP-glucose glycoprotein glucosyltransferase 1
Synonyms: A930007H10Rik, C820010P03Rik, 0910001L17Rik, Ugcgl1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 320011
Homologene: 10586
Hectd4
Name: HECT domain E3 ubiquitin protein ligase 4
Synonyms: Gm15800
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 269700
Homologene: 28297
Plxna2
Name: plexin A2
Synonyms: OCT, Plxn2, 2810428A13Rik, PlexA2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 18845
HGNC: HGNC:9100
Homologene: 56427
Rgs21
Name: regulator of G-protein signalling 21
Synonyms: EG624910
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 624910
Homologene: 82452
Grm6
Name: glutamate receptor, metabotropic 6
Synonyms: mGluR6, nerg1, nob3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 108072
HGNC: HGNC:4598
Homologene: 20232
Dnah11
Name: dynein, axonemal, heavy chain 11
Synonyms: lrd, b2b1279Clo, b2b1203Clo, b2b598Clo, b2b1289Clo, b2b1727Clo, Dnahc11, avc4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 13411
HGNC: HGNC:2942
Homologene: 2801
Pgm5
Name: phosphoglucomutase 5
Synonyms: 9530034F03Rik, aciculin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 226041
HGNC: HGNC:8908
Homologene: 74881
Lrp1b
Name: low density lipoprotein-related protein 1B
Synonyms: 9630004P12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 94217
HGNC: HGNC:6693
Homologene: 56810
Tro
Name: trophinin
Synonyms: necdin and trophinin like, Tnn, trophinin-2, magphinin-gamma, magphinin-beta 2, magphinin-alpha, magphinin, Maged3l, Maged3, Trol
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 56191
Homologene: 75113
Arnt2
Name: aryl hydrocarbon receptor nuclear translocator 2
Synonyms: bHLHe1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 11864
Homologene: 7230
Obscn
Name: obscurin, cytoskeletal calmodulin and titin-interacting RhoGEF
Synonyms: LOC380698, OTTMUSG00000005786
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 380698
Homologene: 70869
Specc1
Name: sperm antigen with calponin homology and coiled-coil domains 1
Synonyms: 2810012G08Rik, B230396K10Rik, Cytsb
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 432572
Homologene: 45157
Csmd2
Name: CUB and Sushi multiple domains 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 329942
Homologene: 89034
Clta
Name: clathrin, light polypeptide (Lca)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 12757
HGNC: HGNC:2090
Homologene: 1384
Gbp3
Name: guanylate binding protein 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 55932
Homologene: 137329
Gbp4
Name: guanylate binding protein 4
Synonyms: Mag-2, Mpa-2, Mpa2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 17472
Homologene: 128731
Rgsl1
Name: regulator of G-protein signaling like 1
Synonyms: 4930415K13Rik, Rgsl2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 240816
Homologene: 129962
Mrpl22
Name: mitochondrial ribosomal protein L22
Synonyms: MRP-L25, Rpml25, HSPC158, E030011D16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 216767
Homologene: 56664
Sgf29
Name: SAGA complex associated factor 29
Synonyms: 1700023O11Rik, Ccdc101
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 75565
Homologene: 12607
Ccdc85a
Name: coiled-coil domain containing 85A
Synonyms: E030025D05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 216613
Homologene: 65605
Fhl4
Name: four and a half LIM domains 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 14202
VEGA: 10
Abcd2
Name: ATP-binding cassette, sub-family D (ALD), member 2
Synonyms: ALDR, ALDL1, adrenoleukodystrophy related, ABC39
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 26874
VEGA: 15
HGNC: HGNC:66
Homologene: 55873
Timm44
Name: translocase of inner mitochondrial membrane 44
Synonyms: Tim44, Mimt44, D8Ertd118e, 0710005E20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 21856
Homologene: 4631
A26c3
Name: ANKRD26-like family C, member 3
Synonyms: 4930430A15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 67575
Homologene: 69410
Nkx3-2
Name: NK3 homeobox 2
Synonyms: Nkx-3.2, Bapx1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 12020
HGNC: HGNC:951
Homologene: 68168
Olfr676
Name: olfactory receptor 676
Synonyms: GA_x6K02T2PBJ9-7664016-7664969, MOR32-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 259099
Homologene: 64955
Txndc9
Name: thioredoxin domain containing 9
Synonyms: ATP binding protein associated with cell differentiation, Apacd
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 98258
Homologene: 4225
Cnga2
Name: cyclic nucleotide gated channel alpha 2
Synonyms: OCNC1, Cnca, Cncg4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 12789
HGNC: HGNC:2149
Homologene: 32022
Gm21370
Name: predicted gene, 21370
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 100861977
VEGA: 13
Pcdha10
Name: protocadherin alpha 10
Synonyms: Cnr8, Cnr3, Crnr8, Crnr3, Pcdha14
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 12943
VEGA: 18
HGNC: HGNC:8666
Homologene: 135720
Xlr5a
Name: X-linked lymphocyte-regulated 5A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 574438
Homologene: 41832
Gm6489
Name: predicted gene 6489
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 624262
Gm13764
Name: predicted gene 13764
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 31,287,239 bp
  • C to T, chromosome 1 at 36,146,668 bp
  • G to T, chromosome 1 at 37,995,790 bp
  • A to G, chromosome 1 at 87,440,826 bp
  • T to C, chromosome 1 at 144,519,875 bp
  • T to A, chromosome 1 at 153,827,536 bp
  • C to T, chromosome 1 at 194,749,317 bp
  • T to C, chromosome 2 at 40,661,489 bp
  • T to C, chromosome 2 at 89,111,572 bp
  • A to G, chromosome 2 at 111,220,702 bp
  • A to T, chromosome 3 at 37,003,933 bp
  • A to C, chromosome 3 at 57,820,589 bp
  • T to A, chromosome 3 at 142,565,355 bp
  • T to G, chromosome 4 at 44,032,417 bp
  • G to A, chromosome 4 at 128,381,945 bp
  • T to G, chromosome 5 at 41,761,961 bp
  • T to A, chromosome 5 at 105,121,907 bp
  • T to G, chromosome 5 at 121,286,257 bp
  • A to G, chromosome 6 at 115,789,466 bp
  • T to A, chromosome 7 at 84,275,345 bp
  • T to C, chromosome 7 at 105,035,303 bp
  • T to C, chromosome 7 at 126,663,938 bp
  • T to C, chromosome 7 at 144,611,742 bp
  • A to C, chromosome 8 at 4,266,654 bp
  • T to A, chromosome 8 at 69,135,695 bp
  • G to A, chromosome 8 at 70,290,143 bp
  • A to T, chromosome 9 at 81,631,539 bp
  • A to G, chromosome 10 at 62,563,027 bp
  • C to T, chromosome 10 at 69,898,256 bp
  • T to C, chromosome 10 at 85,098,455 bp
  • T to C, chromosome 10 at 88,216,031 bp
  • C to T, chromosome 11 at 28,576,686 bp
  • T to C, chromosome 11 at 50,859,989 bp
  • T to A, chromosome 11 at 58,173,102 bp
  • G to A, chromosome 11 at 59,031,591 bp
  • T to G, chromosome 11 at 62,151,827 bp
  • T to G, chromosome 11 at 77,686,229 bp
  • C to T, chromosome 12 at 117,916,896 bp
  • T to A, chromosome 13 at 120,026,842 bp
  • T to C, chromosome 14 at 99,036,490 bp
  • A to T, chromosome 15 at 66,707,942 bp
  • A to G, chromosome 15 at 91,178,283 bp
  • T to C, chromosome 17 at 71,407,235 bp
  • C to A, chromosome 18 at 37,006,916 bp
  • T to C, chromosome 19 at 24,816,445 bp
  • T to A, chromosome X at 72,006,127 bp
  • T to C, chromosome X at 73,157,898 bp
  • T to C, chromosome X at 150,653,325 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4489 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041745-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.