Strain Name:
C57BL/6J-MtgxR4499Btlr/Mmmh
Stock Number:
041752-MU
Citation ID:
RRID:MMRRC_041752-MU
Other Names:
R4499 (G1), C57BL/6J-MtgxR4499Btlr
Major Collection:

Gene Information

Arfgef2
Name: ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited)
Synonyms: BIG2, E230011G24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 99371
Homologene: 111880
Lrrc2
Name: leucine rich repeat containing 2
Synonyms: 2400002D05Rik, 4933431K03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 74249
Homologene: 23454
Fbxl17
Name: F-box and leucine-rich repeat protein 17
Synonyms: C130023C01Rik, Fbx13, 6330576B01Rik, Fbxo13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 50758
Homologene: 79859
Ccdc18
Name: coiled-coil domain containing 18
Synonyms: 4932411G06Rik, 1700021E15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 73254
Homologene: 35455
Slc9a8
Name: solute carrier family 9 (sodium/hydrogen exchanger), member 8
Synonyms: 1200006P13Rik, NHE8, 6430709P13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 77031
Homologene: 75041
Dcaf4
Name: DDB1 and CUL4 associated factor 4
Synonyms: 1110018E21Rik, Wdr21
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 73828
VEGA: 12
Homologene: 9210
Prr11
Name: proline rich 11
Synonyms: B930067F20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 270906
Homologene: 10123
Gm14124
Name: predicted gene 14124
Synonyms: Gm10749, EG640962
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 100216455
HGNC: null
Homologene: 134546
Cdc42bpg
Name: CDC42 binding protein kinase gamma (DMPK-like)
Synonyms: MRCKgamma
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 240505
Homologene: 28384
Vps37b
Name: vacuolar protein sorting 37B
Synonyms: 2300007F24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 330192
Homologene: 11649
Thbs1
Name: thrombospondin 1
Synonyms: TSP-1, tbsp1, TSP1, Thbs-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 21825
Homologene: 31142
Adgrb2
Name: adhesion G protein-coupled receptor B2
Synonyms: Bai2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 230775
HGNC: HGNC:944
Homologene: 1288
Dcc
Name: deleted in colorectal carcinoma
Synonyms: Igdcc1, C030036D22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 13176
HGNC: HGNC:2701
Homologene: 21081
Msh6
Name: mutS homolog 6
Synonyms: Gtmbp, Msh6, GTBP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 17688
VEGA: 17
HGNC: HGNC:7329
Homologene: 149
Adgrl4
Name: adhesion G protein-coupled receptor L4
Synonyms: 1110033N21Rik, EGF-TM7 receptor, Etl, Eltd1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 170757
Homologene: 11170
Dpep2
Name: dipeptidase 2
Synonyms: F630103D06Rik, MBD-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 319446
Homologene: 49703
Mesd
Name: mesoderm development LRP chaperone
Synonyms: Mesdc2, msd, 2210015O11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 67943
Homologene: 11347
Ice1
Name: interactor of little elongation complex ELL subunit 1
Synonyms: BC018507
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 218333
VEGA: 13
Homologene: 18902
Ssh2
Name: slingshot protein phosphatase 2
Synonyms: SSH-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 237860
Homologene: 14116
Ttn
Name: titin
Synonyms: shru, connectin, mdm, 2310057K23Rik, D330041I19Rik, 1100001C23Rik, D830007G01Rik, 2310036G12Rik, L56, 2310074I15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 22138
Homologene: 130650
Camta2
Name: calmodulin binding transcription activator 2
Synonyms: Kiaa0909-hp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 216874
Homologene: 9021
Dennd4a
Name: DENN/MADD domain containing 4A
Synonyms: F730015K02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 102442
VEGA: 9
Homologene: 55933
Xirp2
Name: xin actin-binding repeat containing 2
Synonyms: A530024P18Rik, myomaxin, Cmya3, 2310008C07Rik, mXin beta, 2310003D02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 241431
Homologene: 19388
Bpifb5
Name: BPI fold containing family B, member 5
Synonyms: BC018465
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 228802
Homologene: 64814
Stard9
Name: START domain containing 9
Synonyms: E230025N21Rik, 4831403C07Rik, Kif16a, N-3 kinesin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 668880
Homologene: 130712
Akna
Name: AT-hook transcription factor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 100182
Homologene: 49947
Agbl3
Name: ATP/GTP binding protein-like 3
Synonyms: 4930431N21Rik, Ccp3, 2900053G10Rik, 6530406M24Rik, Ccp3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 76223
Homologene: 35330
Slc16a7
Name: solute carrier family 16 (monocarboxylic acid transporters), member 7
Synonyms: 9030411M13Rik, MCT2, D630004K10Rik, 4921534N07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 20503
Homologene: 20990
Ache
Name: acetylcholinesterase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 11423
HGNC: HGNC:108
Homologene: 543
Zfp53
Name: zinc finger protein 53
Synonyms: KRAZ1, Zfp-53, zfas8, Zfp118, D030067O06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 24132
VEGA: 17
HGNC: null
Homologene: 135946
Cep126
Name: centrosomal protein 126
Synonyms: AK129341
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 234915
VEGA: 9
Homologene: 28313
Stk19
Name: serine/threonine kinase 19
Synonyms: G11, RP1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 54402
Homologene: 10449
Nod1
Name: nucleotide-binding oligomerization domain containing 1
Synonyms: Nlrc1, Card4, F830007N14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 107607
Homologene: 4440
Myo15b
Name: myosin XVB
Synonyms: LOC380737, LOC217328, E330039G21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 217328
Homologene: null
Nrap
Name: nebulin-related anchoring protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 18175
HGNC: HGNC:7988
Homologene: 4499
Zswim8
Name: zinc finger SWIM-type containing 8
Synonyms: 2310021P13Rik, 4832404P21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 268721
VEGA: 14
Homologene: 34313
Dgkq
Name: diacylglycerol kinase, theta
Synonyms: DAGK7, Dgk theta, 110kDa, Dgkd, Dagk4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 110524
HGNC: HGNC:2856
Homologene: 20352
Bank1
Name: B cell scaffold protein with ankyrin repeats 1
Synonyms: A530094C12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 242248
Homologene: 9926
2210408I21Rik
Name: RIKEN cDNA 2210408I21 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 72371
Homologene: 89234
Slc47a1
Name: solute carrier family 47, member 1
Synonyms: MATE1, 1300013J15Rik, mMATE1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 67473
Homologene: 34364
Rusc1
Name: RUN and SH3 domain containing 1
Synonyms: 2210403N08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 72296
Homologene: 75028
Fmo3
Name: flavin containing monooxygenase 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 14262
HGNC: HGNC:3771
Homologene: 128199
P2ry12
Name: purinergic receptor P2Y, G-protein coupled 12
Synonyms: P2Y12, 2900079B22Rik, 4921504D23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 70839
Homologene: 11260
Psg25
Name: pregnancy-specific glycoprotein 25
Synonyms: cea13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 114868
HGNC: null
Homologene: 110989
Alg6
Name: asparagine-linked glycosylation 6 (alpha-1,3,-glucosyltransferase)
Synonyms: E230028F23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 320438
Homologene: 6920
Asnsd1
Name: asparagine synthetase domain containing 1
Synonyms: 2210409M21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 70396
Homologene: 6564
D10Bwg1379e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
Gm8221
Name: predicted gene 8221
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 666661
VEGA: 15
HGNC: null
Homologene: null
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 53,347,970 bp
  • T to C, chromosome 1 at 162,963,966 bp
  • A to T, chromosome 2 at 67,513,438 bp
  • T to A, chromosome 2 at 76,916,478 bp
  • T to C, chromosome 2 at 118,119,950 bp
  • T to A, chromosome 2 at 120,700,241 bp
  • T to G, chromosome 2 at 150,269,442 bp
  • A to T, chromosome 2 at 154,240,758 bp
  • G to A, chromosome 2 at 166,885,814 bp
  • T to A, chromosome 2 at 167,424,193 bp
  • A to G, chromosome 3 at 59,217,657 bp
  • T to C, chromosome 3 at 89,092,308 bp
  • T to C, chromosome 3 at 136,284,243 bp
  • A to G, chromosome 3 at 151,510,785 bp
  • T to C, chromosome 4 at 63,395,041 bp
  • A to T, chromosome 4 at 99,761,918 bp
  • A to T, chromosome 4 at 129,992,661 bp
  • T to G, chromosome 5 at 108,228,960 bp
  • C to G, chromosome 5 at 108,649,661 bp
  • T to C, chromosome 5 at 124,007,626 bp
  • A to C, chromosome 5 at 137,291,932 bp
  • T to C, chromosome 6 at 34,857,598 bp
  • T to A, chromosome 6 at 54,943,996 bp
  • C to T, chromosome 7 at 18,524,891 bp
  • G to A, chromosome 7 at 83,897,977 bp
  • T to C, chromosome 8 at 105,985,482 bp
  • T to C, chromosome 9 at 8,101,588 bp
  • G to A, chromosome 9 at 64,910,123 bp
  • A to T, chromosome 9 at 110,962,645 bp
  • T to C, chromosome 10 at 18,608,343 bp
  • T to C, chromosome 10 at 125,228,187 bp
  • T to A, chromosome 11 at 61,359,529 bp
  • T to C, chromosome 11 at 70,674,686 bp
  • T to A, chromosome 11 at 77,393,067 bp
  • T to G, chromosome 11 at 87,098,707 bp
  • A to G, chromosome 11 at 115,890,952 bp
  • T to A, chromosome 12 at 83,539,360 bp
  • T to C, chromosome 13 at 70,609,027 bp
  • T to A, chromosome 13 at 77,316,527 bp
  • T to A, chromosome 14 at 20,714,297 bp
  • T to A, chromosome 15 at 77,626,045 bp
  • A to G, chromosome 17 at 21,509,235 bp
  • T to C, chromosome 17 at 34,824,251 bp
  • A to C, chromosome 17 at 63,080,523 bp
  • T to A, chromosome 17 at 87,980,269 bp
  • A to T, chromosome 18 at 71,547,317 bp
  • C to T, chromosome 19 at 6,320,555 bp
  • G to A, chromosome 19 at 56,351,481 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4499 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041752-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.