Strain Name:
C57BL/6J-MtgxR4520Btlr/Mmmh
Stock Number:
041763-MU
Citation ID:
RRID:MMRRC_041763-MU
Other Names:
R4520 (G1), C57BL/6J-MtgxR4520Btlr
Major Collection:

Gene Information

Esr2
Name: estrogen receptor 2 (beta)
Synonyms: ER beta, Estrb, ERbeta, oestrogen receptor beta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 13983
HGNC: HGNC:3468
Homologene: 1100
Mga
Name: MAX gene associated
Synonyms: Mga, C130042M01Rik, D030062C11Rik, Mad5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 29808
Homologene: 49351
Ccdc88c
Name: coiled-coil domain containing 88C
Synonyms: 0610010D24Rik, Daple
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 68339
VEGA: 12
Homologene: 18903
Ascc3
Name: activating signal cointegrator 1 complex subunit 3
Synonyms: ASC1p200, Helic1, B630009I04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 77987
VEGA: 10
Homologene: 4973
Clic4
Name: chloride intracellular channel 4 (mitochondrial)
Synonyms: D0Jmb3, mc3s5, mtCLIC
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 29876
Homologene: 8490
Sspn
Name: sarcospan
Synonyms: Krag
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 16651
Homologene: 3727
Marf1
Name: meiosis regulator and mRNA stability 1
Synonyms: 4921513D23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 223989
VEGA: 16
Homologene: 40967
Slc6a3
Name: solute carrier family 6 (neurotransmitter transporter, dopamine), member 3
Synonyms: DAT, Dat1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 13162
VEGA: 13
Homologene: 55547
Pole
Name: polymerase (DNA directed), epsilon
Synonyms: pol-epsilon
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 18973
HGNC: HGNC:9177
Homologene: 4539
Golga3
Name: golgi autoantigen, golgin subfamily a, 3
Synonyms: 5430416E01Rik, repro27, G1-499-14, Mea2, Mea-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 269682
HGNC: HGNC:4426
Homologene: 4308
Pabpc1
Name: poly(A) binding protein, cytoplasmic 1
Synonyms: Pabpl1, Pabp1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 18458
HGNC: HGNC:8554
Homologene: 37638
Cspp1
Name: centrosome and spindle pole associated protein 1
Synonyms: 2310020J12Rik, 4930413O22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 211660
Homologene: 23487
Ntng1
Name: netrin G1
Synonyms: Lmnt1, A930010C08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 80883
Homologene: 8949
Degs1
Name: delta(4)-desaturase, sphingolipid 1
Synonyms: Des1, Mdes
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 13244
Homologene: 55770
Rpl10l
Name: ribosomal protein L10-like
Synonyms: EG238217
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 238217
Homologene: 68830
Tlr8
Name: toll-like receptor 8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 170744
Homologene: 44054
Dnhd1
Name: dynein heavy chain domain 1
Synonyms: 8030491N06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 77505
Homologene: 131117
Scn1a
Name: sodium channel, voltage-gated, type I, alpha
Synonyms: Nav1.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 20265
Homologene: 21375
Pigo
Name: phosphatidylinositol glycan anchor biosynthesis, class O
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 56703
Homologene: 31761
Naxe
Name: NAD(P)HX epimerase
Synonyms: AI-BP, APOA1BP, Apoa1bp, ESTM37
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 246703
Homologene: 70948
Zcchc14
Name: zinc finger, CCHC domain containing 14
Synonyms: Bdg29
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 142682
Homologene: 9037
1110002E22Rik
Name: RIKEN cDNA 1110002E22 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 102634333
Homologene: 131709
Otog
Name: otogelin
Synonyms: Otgn
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 18419
HGNC: HGNC:8516
Homologene: 8421
Cyp2b10
Name: cytochrome P450, family 2, subfamily b, polypeptide 10
Synonyms: phenobarbitol inducible, type b, Cyp2b20, Cyp2b, p16
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 13088
HGNC: HGNC:2615
Homologene: 73894
Olfr1097
Name: olfactory receptor 1097
Synonyms: MOR206-2, GA_x6K02T2Q125-48376288-48375341
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258840
Homologene: 37005
Rpgrip1
Name: retinitis pigmentosa GTPase regulator interacting protein 1
Synonyms: 4930505G06Rik, nmf247, A930002K18Rik, 4930401L23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 77945
Homologene: 10679
Ihh
Name: Indian hedgehog
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 16147
HGNC: HGNC:5956
Homologene: 22586
Plce1
Name: phospholipase C, epsilon 1
Synonyms: 4933403A21Rik, PLCepsilon
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 74055
Homologene: 9478
Vmn2r84
Name: vomeronasal 2, receptor 84
Synonyms: EG625068
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 625068
Homologene: 129606
Serpinb9c
Name: serine (or cysteine) peptidase inhibitor, clade B, member 9c
Synonyms: NK9, Spi11, ovalbumin, 3830421J05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 20707
HGNC: HGNC:8955
Rufy4
Name: RUN and FYVE domain containing 4
Synonyms: F930048N03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 435626
Homologene: 52359
Krt13
Name: keratin 13
Synonyms: Krt-1.13, Krt1-13, K13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 16663
HGNC: HGNC:6415
Homologene: 40740
Cyp2c50
Name: cytochrome P450, family 2, subfamily c, polypeptide 50
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 107141
Homologene: 137231
Fbxo41
Name: F-box protein 41
Synonyms: D6Ertd538e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 330369
Homologene: 19837
Mrpl23
Name: mitochondrial ribosomal protein L23
Synonyms: Rpl23l, L23mrp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 19935
Homologene: 7922
Mterf1a
Name: mitochondrial transcription termination factor 1a
Synonyms: 4931431L11Rik, Mterf, Mterf1, 9230106K09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 545725
Homologene: 5073
Psg28
Name: pregnancy-specific glycoprotein 28
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 114871
Homologene: 110989
Lrrc39
Name: leucine rich repeat containing 39
Synonyms: 9430028I06Rik, 2010005E21Rik, Myomasp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 109245
Homologene: 13668
Slc13a1
Name: solute carrier family 13 (sodium/sulfate symporters), member 1
Synonyms: Nas1, NaSi-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 55961
Homologene: 31893
Cel
Name: carboxyl ester lipase
Synonyms: BAL, 1810036E18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 12613
HGNC: HGNC:1848
Homologene: 37529
Trim15
Name: tripartite motif-containing 15
Synonyms: 1810012B10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 69097
Homologene: 23819
Ido2
Name: indoleamine 2,3-dioxygenase 2
Synonyms: C230043N17Rik, Indol1, Ido2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 209176
Homologene: 48830
Gtpbp6
Name: GTP binding protein 6 (putative)
Synonyms: Pgbpll, pgpl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 107999
Homologene: 8157
Gm7461
Name: predicted gene 7461
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Olfr572
Name: olfactory receptor 572
Synonyms: GA_x6K02T2PBJ9-5638785-5639741, MOR10-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 259093
Homologene: 131142
Olfr657
Name: olfactory receptor 657
Synonyms: GA_x6K02T2PBJ9-7263864-7264823, MOR40-13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 258309
Homologene: 17189
Sh2d6
Name: SH2 domain containing 6
Synonyms: 4933424C13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 71130
Homologene: 65986
Gm5444
Name: predicted gene 5444
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Shisa8
Name: shisa family member 8
Synonyms: Orf26, CKAMP39, AI848285
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 435145
VEGA: 15
Homologene: 46536
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 10,134,227 bp
  • T to C, chromosome 1 at 74,147,663 bp
  • T to A, chromosome 1 at 74,950,950 bp
  • T to C, chromosome 1 at 182,276,808 bp
  • C to T, chromosome 2 at 28,557,968 bp
  • T to C, chromosome 2 at 66,273,731 bp
  • T to C, chromosome 2 at 86,891,019 bp
  • T to A, chromosome 2 at 119,948,098 bp
  • A to G, chromosome 3 at 88,057,982 bp
  • A to G, chromosome 3 at 109,934,996 bp
  • A to T, chromosome 3 at 116,581,613 bp
  • A to G, chromosome 3 at 138,070,266 bp
  • A to T, chromosome 4 at 43,020,301 bp
  • T to C, chromosome 4 at 135,223,480 bp
  • A to G, chromosome 5 at 3,890,992 bp
  • T to C, chromosome 5 at 110,107,859 bp
  • T to A, chromosome 5 at 110,203,751 bp
  • T to A, chromosome 5 at 110,297,924 bp
  • T to C, chromosome 6 at 24,134,513 bp
  • T to C, chromosome 6 at 72,518,953 bp
  • A to G, chromosome 6 at 85,484,042 bp
  • A to G, chromosome 6 at 145,961,307 bp
  • T to A, chromosome 7 at 18,422,901 bp
  • T to C, chromosome 7 at 25,911,557 bp
  • T to A, chromosome 7 at 46,241,053 bp
  • A to T, chromosome 7 at 102,928,557 bp
  • T to A, chromosome 7 at 104,636,169 bp
  • A to T, chromosome 7 at 105,674,370 bp
  • T to A, chromosome 7 at 142,540,678 bp
  • G to T, chromosome 8 at 4,677,673 bp
  • T to C, chromosome 8 at 24,576,178 bp
  • A to T, chromosome 8 at 121,609,095 bp
  • A to G, chromosome 10 at 50,660,670 bp
  • T to A, chromosome 10 at 130,386,522 bp
  • A to G, chromosome 11 at 100,119,348 bp
  • T to C, chromosome 12 at 66,283,738 bp
  • G to A, chromosome 12 at 76,167,549 bp
  • G to T, chromosome 12 at 100,913,332 bp
  • A to G, chromosome 13 at 4,834,226 bp
  • T to C, chromosome 13 at 33,151,857 bp
  • G to A, chromosome 13 at 73,540,856 bp
  • A to G, chromosome 14 at 52,152,289 bp
  • A to G, chromosome 15 at 36,608,513 bp
  • C to G, chromosome 15 at 82,211,962 bp
  • T to C, chromosome 16 at 14,132,666 bp
  • T to C, chromosome 17 at 36,866,350 bp
  • T to C, chromosome 19 at 38,524,319 bp
  • A to G, chromosome 19 at 40,090,689 bp
  • C to T, chromosome X at 167,243,175 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4520 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041763-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.