Strain Name:
C57BL/6J-MtgxR4528Btlr/Mmmh
Stock Number:
041769-MU
Citation ID:
RRID:MMRRC_041769-MU
Other Names:
R4528 (G1), C57BL/6J-MtgxR4528Btlr
Major Collection:

Gene Information

Kmt2e
Name: lysine (K)-specific methyltransferase 2E
Synonyms: 1810033J14Rik, 9530077A04Rik, D230038D11Rik, Mll5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 69188
Homologene: 18822
Atm
Name: ataxia telangiectasia mutated
Synonyms: C030026E19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 11920
HGNC: HGNC:795
Homologene: 30952
Lims1
Name: LIM and senescent cell antigen-like domains 1
Synonyms: 4921524A02Rik, 2310016J22Rik, C430041B13Rik, Lims1l, PINCH1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 110829
HGNC: HGNC:6616
Homologene: 68428
Camsap3
Name: calmodulin regulated spectrin-associated protein family, member 3
Synonyms: Nezha, 2310057J16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 69697
Homologene: 18966
Zbtb43
Name: zinc finger and BTB domain containing 43
Synonyms: Zfp297b, 1700010E06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 71834
Homologene: 8514
Usp5
Name: ubiquitin specific peptidase 5 (isopeptidase T)
Synonyms: Ucht
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 22225
Homologene: 55758
Dcaf1
Name: DDB1 and CUL4 associated factor 1
Synonyms: B930007L02Rik, Vprbp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 321006
Homologene: 8805
Mki67
Name: antigen identified by monoclonal antibody Ki 67
Synonyms: D630048A14Rik, Ki67, Ki-67
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 17345
HGNC: HGNC:7107
Homologene: 1814
Zfp985
Name: zinc finger protein 985
Synonyms: Gm13154
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 433804
Homologene: 133076
Pex1
Name: peroxisomal biogenesis factor 1
Synonyms: peroxisome biogenesis factor 1, 5430414H02Rik, E330005K07Rik, ZWS1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 71382
HGNC: HGNC:8850
Homologene: 27006
Hr
Name: lysine demethylase and nuclear receptor corepressor
Synonyms: N, rh, rh-bmh, ba, bldy
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 15460
HGNC: HGNC:5172
Homologene: 3774
Strip2
Name: striatin interacting protein 2
Synonyms: D330017J20Rik, Fam40b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 320609
Homologene: 66198
Dnhd1
Name: dynein heavy chain domain 1
Synonyms: 8030491N06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 77505
Homologene: 131117
Idh2
Name: isocitrate dehydrogenase 2 (NADP+), mitochondrial
Synonyms: Idh-2, IDPm
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 269951
HGNC: HGNC:5383
Homologene: 37590
Col7a1
Name: collagen, type VII, alpha 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 12836
HGNC: HGNC:2214
Homologene: 73
Uba7
Name: ubiquitin-like modifier activating enzyme 7
Synonyms: 1300004C08Rik, Ube1l
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 74153
Homologene: 2502
Fat4
Name: FAT atypical cadherin 4
Synonyms: 6030410K14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 329628
Homologene: 14377
Scube3
Name: signal peptide, CUB domain, EGF-like 3
Synonyms: D030038I21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 268935
Homologene: 65059
Gbe1
Name: glucan (1,4-alpha-), branching enzyme 1
Synonyms: 2310045H19Rik, 2810426P10Rik, D16Ertd536e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 74185
HGNC: HGNC:4180
Homologene: 129
Pde4dip
Name: phosphodiesterase 4D interacting protein (myomegalin)
Synonyms: 9430063L05Rik, D3Bwg1078e, D130016K21Rik, Usmg4, 4732458A06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 83679
Homologene: 66961
Mrto4
Name: mRNA turnover 4, ribosome maturation factor
Synonyms: Mrt4, 2610012O22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 69902
Homologene: 6379
Olfr1308
Name: olfactory receptor 1308
Synonyms: GA_x6K02T2Q125-73008844-73007882, MOR245-22
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 258258
Homologene: 45797
Olfr417
Name: olfactory receptor 417
Synonyms: GA_x6K02T2P20D-20787051-20786119, MOR267-11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 258238
Homologene: 17358
Pus1
Name: pseudouridine synthase 1
Synonyms: A730013B20Rik, MPUS1, mPus1p
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 56361
Homologene: 5931
Ugt1a6b
Name: UDP glucuronosyltransferase 1 family, polypeptide A6B
Synonyms: A9'
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 394435
Homologene: 85959
Coro1b
Name: coronin, actin binding protein 1B
Synonyms: coronin 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 23789
HGNC: HGNC:2253
Homologene: 40790
Serpina11
Name: serine (or cysteine) peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 11
Synonyms: LOC380780
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 380780
Homologene: 28490
Foxred2
Name: FAD-dependent oxidoreductase domain containing 2
Synonyms: A430097D04Rik, D15Bwg0759e, LOC239554
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 239554
Homologene: 11800
Cenpu
Name: centromere protein U
Synonyms: Mlf1ip, 1700029A22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 71876
Homologene: 11629
Gm1527
Name: predicted gene 1527
Synonyms: LOC385263
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 385263
Homologene: 133976
Plscr2
Name: phospholipid scramblase 2
Synonyms: PL scramblase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 18828
Homologene: 134152
Zfpm1
Name: zinc finger protein, multitype 1
Synonyms: Fog1, Friend of GATA-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 22761
Homologene: 7606
Cpeb3
Name: cytoplasmic polyadenylation element binding protein 3
Synonyms: 4831444O18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 208922
Homologene: 40992
Olfr574
Name: olfactory receptor 574
Synonyms: MOR14-9, GA_x6K02T2PBJ9-5659738-5660748
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 258357
Homologene: 27137
Tlr12
Name: toll-like receptor 12
Synonyms: LOC384059, Tlr11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 384059
Homologene: 135964
Etfa
Name: electron transferring flavoprotein, alpha polypeptide
Synonyms: D9Ertd394e, 2010200I21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 110842
HGNC: HGNC:3481
Homologene: 100
Gm14410
Name: predicted gene 14410
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 100043403
Cyp2a22
Name: cytochrome P450, family 2, subfamily a, polypeptide 22
Synonyms: EG233005
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 233005
Homologene: 69128
Thtpa
Name: thiamine triphosphatase
Synonyms: ThTPase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 105663
Homologene: 17691
Olfr1174-ps
Name: olfactory receptor 1174, pseudogene
Synonyms: MOR174-18, GA_x6K02T2Q125-49796506-49795568
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 546769
Homologene: 77381
Gm26529
Name: predicted gene, 26529
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
Gm19224
Name: predicted gene, 19224
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 100418458
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 88,107,579 bp
  • T to A, chromosome 1 at 174,369,256 bp
  • A to G, chromosome 2 at 33,462,325 bp
  • C to A, chromosome 2 at 88,311,553 bp
  • T to C, chromosome 2 at 111,960,948 bp
  • G to T, chromosome 2 at 177,193,941 bp
  • A to T, chromosome 2 at 177,193,943 bp
  • T to A, chromosome 3 at 28,914,393 bp
  • T to A, chromosome 3 at 38,891,294 bp
  • C to T, chromosome 3 at 97,717,022 bp
  • A to C, chromosome 4 at 128,618,025 bp
  • A to G, chromosome 4 at 139,348,723 bp
  • A to T, chromosome 4 at 147,582,890 bp
  • T to C, chromosome 5 at 3,631,712 bp
  • T to C, chromosome 5 at 23,473,558 bp
  • T to C, chromosome 5 at 110,774,730 bp
  • T to C, chromosome 6 at 29,933,049 bp
  • C to G, chromosome 6 at 124,822,630 bp
  • T to C, chromosome 7 at 26,934,769 bp
  • GGCCAGC to G, chromosome 7 at 80,098,349 bp
  • T to C, chromosome 7 at 89,981,194 bp
  • A to G, chromosome 7 at 102,948,806 bp
  • A to G, chromosome 7 at 105,682,806 bp
  • A to T, chromosome 7 at 135,695,359 bp
  • A to G, chromosome 8 at 3,606,515 bp
  • G to T, chromosome 8 at 46,562,422 bp
  • G to A, chromosome 8 at 122,335,642 bp
  • A to T, chromosome 9 at 53,500,759 bp
  • A to G, chromosome 9 at 55,500,050 bp
  • G to A, chromosome 9 at 92,289,693 bp
  • A to T, chromosome 9 at 106,844,204 bp
  • A to G, chromosome 9 at 107,983,903 bp
  • C to A, chromosome 9 at 108,959,533 bp
  • T to C, chromosome 10 at 58,410,060 bp
  • A to T, chromosome 12 at 103,986,333 bp
  • T to A, chromosome 14 at 55,095,582 bp
  • T to C, chromosome 14 at 70,566,383 bp
  • T to A, chromosome 15 at 77,943,249 bp
  • A to T, chromosome 16 at 70,478,337 bp
  • T to A, chromosome 17 at 28,162,999 bp
  • T to C, chromosome 19 at 4,149,981 bp
  • T to A, chromosome 19 at 11,703,936 bp
  • T to C, chromosome 19 at 37,139,088 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4528 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041769-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.