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Strain Name:
C57BL/6J-MtgxR4572Btlr/Mmmh
Stock Number:
041796-MU
Citation ID:
RRID:MMRRC_041796-MU
Other Names:
R4572 (G1), C57BL/6J-MtgxR4572Btlr
Major Collection:

Strain Information

Rgs14
Name: regulator of G-protein signaling 14
Synonyms: Rap1/rap2 interacting protein
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 51791
HGNC: HGNC:9996
Homologene: 4735
Rpa1
Name: replication protein A1
Synonyms: Rpa, RF-A, RP-A, 5031405K23Rik, 70kDa
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 68275
Homologene: 2208
Zmym1
Name: zinc finger, MYM domain containing 1
Synonyms: 5830412B09Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 68310
Homologene: 32904
Phf14
Name: PHD finger protein 14
Synonyms: 1110001C23Rik, 4932409F11Rik, 5730446A07Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 75725
Homologene: 8775
Plxnd1
Name: plexin D1
Synonyms: 6230425C21Rik, b2b553Clo, b2b1863Clo
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 67784
HGNC: HGNC:9107
Homologene: 22866
Wnk1
Name: WNK lysine deficient protein kinase 1
Synonyms: 6430573H23Rik, Prkwnk1, EG406236, Hsn2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 232341
Homologene: 14253
Cnot2
Name: CCR4-NOT transcription complex, subunit 2
Synonyms: 2600016M12Rik, 2810470K03Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 72068
HGNC: HGNC:7878
Homologene: 40953
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 10,213,141 bp
  • T to C, chromosome 1 at 187,193,996 bp
  • A to G, chromosome 2 at 27,083,256 bp
  • G to A, chromosome 2 at 119,402,358 bp
  • T to A, chromosome 2 at 122,281,726 bp
  • G to A, chromosome 2 at 152,318,733 bp
  • A to G, chromosome 2 at 156,177,847 bp
  • A to T, chromosome 3 at 65,783,915 bp
  • A to T, chromosome 4 at 49,514,399 bp
  • A to G, chromosome 4 at 61,438,217 bp
  • A to G, chromosome 4 at 124,902,568 bp
  • A to T, chromosome 4 at 127,050,835 bp
  • A to C, chromosome 4 at 136,655,940 bp
  • A to G, chromosome 5 at 108,332,885 bp
  • G to A, chromosome 6 at 12,006,824 bp
  • T to A, chromosome 6 at 28,430,635 bp
  • A to T, chromosome 6 at 65,454,991 bp
  • C to A, chromosome 6 at 115,955,756 bp
  • T to C, chromosome 6 at 119,951,911 bp
  • T to C, chromosome 6 at 122,581,397 bp
  • A to G, chromosome 7 at 19,584,464 bp
  • G to A, chromosome 7 at 20,026,681 bp
  • G to A, chromosome 7 at 41,216,159 bp
  • G to T, chromosome 7 at 104,411,188 bp
  • G to A, chromosome 7 at 143,781,104 bp
  • A to G, chromosome 8 at 61,987,421 bp
  • A to G, chromosome 8 at 64,056,309 bp
  • A to G, chromosome 8 at 69,882,410 bp
  • A to G, chromosome 8 at 112,012,017 bp
  • A to T, chromosome 8 at 123,834,060 bp
  • G to A, chromosome 9 at 14,321,220 bp
  • A to C, chromosome 9 at 19,883,979 bp
  • A to T, chromosome 9 at 21,280,384 bp
  • A to G, chromosome 9 at 27,094,615 bp
  • T to C, chromosome 9 at 114,384,806 bp
  • T to C, chromosome 9 at 121,192,764 bp
  • T to C, chromosome 10 at 9,838,144 bp
  • T to A, chromosome 10 at 79,866,063 bp
  • T to C, chromosome 10 at 116,494,846 bp
  • A to T, chromosome 11 at 5,689,340 bp
  • CTCTTTCT to CTCT, chromosome 11 at 6,274,461 bp
  • A to T, chromosome 11 at 9,007,617 bp
  • A to G, chromosome 11 at 42,593,917 bp
  • A to G, chromosome 11 at 70,321,181 bp
  • A to G, chromosome 11 at 73,689,785 bp
  • A to G, chromosome 11 at 75,316,236 bp
  • A to T, chromosome 11 at 83,121,463 bp
  • A to C, chromosome 11 at 97,534,934 bp
  • G to A, chromosome 11 at 103,732,155 bp
  • A to T, chromosome 11 at 110,216,548 bp
  • A to G, chromosome 12 at 11,242,418 bp
  • A to T, chromosome 12 at 103,898,708 bp
  • A to T, chromosome 12 at 118,010,125 bp
  • A to T, chromosome 13 at 55,380,062 bp
  • A to G, chromosome 14 at 64,903,233 bp
  • T to C, chromosome 14 at 66,307,873 bp
  • A to C, chromosome 15 at 5,243,133 bp
  • T to A, chromosome 15 at 9,306,393 bp
  • G to T, chromosome 15 at 72,937,067 bp
  • T to C, chromosome 15 at 75,998,750 bp
  • A to G, chromosome 16 at 20,712,139 bp
  • T to C, chromosome 16 at 32,753,802 bp
  • A to T, chromosome 16 at 34,392,042 bp
  • A to T, chromosome 16 at 59,461,638 bp
  • A to T, chromosome 18 at 44,275,917 bp
  • A to T, chromosome 18 at 65,281,004 bp
  • T to A, chromosome 18 at 89,473,947 bp
  • A to G, chromosome 19 at 46,670,551 bp
  • C to T, chromosome X at 25,560,356 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4572 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041796-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.