Strain Name:
C57BL/6J-MtgxR4576Btlr/Mmmh
Stock Number:
041799-MU
Citation ID:
RRID:MMRRC_041799-MU
Other Names:
R4576 (G1), C57BL/6J-MtgxR4576Btlr
Major Collection:

Gene Information

Gene Symbol: Fzd9 [MGI:1313278] (Mus musculus (mouse))
Name: frizzled class receptor 9
Synonyms: Fz9, mfz9, frizzled 9
Chromosome: 5
Alteration at locus: Chemically Induced
Gene Symbol: Ppp1r14c [MGI:1923392] (Mus musculus (mouse))
Name: protein phosphatase 1, regulatory inhibitor subunit 14C
Synonyms: KEPI, 6330514J04Rik
Chromosome: 10
Alteration at locus: Chemically Induced
Gene Symbol: Enah [MGI:108360] (Mus musculus (mouse))
Name: ENAH actin regulator
Synonyms: Ndpp1, Mena
Chromosome: 1
Alteration at locus: Chemically Induced
Gene Symbol: Plxnd1 [MGI:2154244] (Mus musculus (mouse))
Name: plexin D1
Synonyms: 6230425C21Rik, b2b553Clo, b2b1863Clo
Chromosome: 6
Alteration at locus: Chemically Induced
Gene Symbol: Zc3h4 [MGI:2682314] (Mus musculus (mouse))
Name: zinc finger CCCH-type containing 4
Synonyms: Kiaa1064-hp, Bwq1, LOC330474
Chromosome: 7
Alteration at locus: Chemically Induced
Gene Symbol: Foxj3 [MGI:2443432] (Mus musculus (mouse))
Name: forkhead box J3
Synonyms: C330039G02Rik, Fhd6
Chromosome: 4
Alteration at locus: Chemically Induced
Gene Symbol: Auts2 [MGI:1919847] (Mus musculus (mouse))
Name: autism susceptibility candidate 2
Synonyms: A730011F23Rik, 2700063G02Rik, D830032G16Rik
Chromosome: 5
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 9,553,754 bp
  • T to A, chromosome 1 at 96,988,697 bp
  • A to G, chromosome 1 at 97,792,339 bp
  • G to T, chromosome 1 at 150,734,487 bp
  • G to T, chromosome 1 at 175,686,940 bp
  • G to A, chromosome 1 at 181,919,563 bp
  • T to C, chromosome 2 at 25,220,288 bp
  • A to T, chromosome 2 at 26,431,119 bp
  • A to T, chromosome 2 at 32,770,059 bp
  • C to T, chromosome 2 at 93,468,669 bp
  • G to A, chromosome 2 at 118,119,416 bp
  • A to T, chromosome 2 at 157,101,750 bp
  • A to T, chromosome 2 at 177,475,680 bp
  • G to A, chromosome 3 at 51,856,506 bp
  • A to G, chromosome 3 at 68,950,530 bp
  • C to A, chromosome 3 at 97,754,249 bp
  • A to T, chromosome 4 at 58,834,708 bp
  • A to G, chromosome 4 at 119,621,663 bp
  • G to T, chromosome 4 at 135,561,251 bp
  • A to T, chromosome 4 at 149,994,476 bp
  • T to A, chromosome 5 at 33,661,497 bp
  • C to T, chromosome 5 at 43,979,464 bp
  • A to G, chromosome 5 at 45,681,789 bp
  • T to C, chromosome 5 at 67,815,815 bp
  • A to G, chromosome 5 at 108,644,602 bp
  • A to T, chromosome 5 at 109,363,799 bp
  • C to T, chromosome 5 at 114,065,682 bp
  • T to G, chromosome 5 at 123,765,955 bp
  • C to T, chromosome 5 at 132,258,934 bp
  • A to G, chromosome 5 at 135,250,312 bp
  • C to A, chromosome 5 at 139,185,639 bp
  • T to C, chromosome 5 at 139,972,053 bp
  • G to A, chromosome 6 at 115,968,044 bp
  • A to G, chromosome 7 at 11,846,769 bp
  • G to A, chromosome 7 at 16,434,654 bp
  • A to G, chromosome 7 at 30,717,722 bp
  • A to G, chromosome 7 at 43,773,243 bp
  • T to C, chromosome 7 at 67,240,439 bp
  • T to C, chromosome 7 at 104,357,331 bp
  • A to G, chromosome 7 at 139,978,130 bp
  • T to A, chromosome 7 at 140,811,590 bp
  • T to C, chromosome 7 at 141,104,138 bp
  • T to C, chromosome 7 at 141,457,344 bp
  • A to T, chromosome 8 at 25,890,020 bp
  • T to C, chromosome 8 at 70,182,063 bp
  • A to G, chromosome 9 at 9,673,292 bp
  • T to C, chromosome 9 at 35,214,608 bp
  • A to G, chromosome 9 at 36,923,548 bp
  • G to T, chromosome 9 at 62,838,949 bp
  • T to C, chromosome 9 at 95,405,171 bp
  • G to T, chromosome 10 at 3,366,912 bp
  • A to G, chromosome 10 at 39,634,654 bp
  • T to C, chromosome 10 at 62,385,843 bp
  • A to G, chromosome 10 at 80,170,241 bp
  • C to T, chromosome 10 at 93,043,228 bp
  • ACAGGCGC to AC, chromosome 10 at 127,540,188 bp
  • T to A, chromosome 11 at 83,765,000 bp
  • T to C, chromosome 11 at 116,289,183 bp
  • A to T, chromosome 12 at 30,011,923 bp
  • G to A, chromosome 12 at 101,886,240 bp
  • T to C, chromosome 12 at 103,001,803 bp
  • C to T, chromosome 13 at 22,009,552 bp
  • C to T, chromosome 13 at 38,181,160 bp
  • C to A, chromosome 13 at 59,716,861 bp
  • A to T, chromosome 13 at 60,721,822 bp
  • T to C, chromosome 13 at 89,319,731 bp
  • T to C, chromosome 13 at 91,896,410 bp
  • A to G, chromosome 14 at 36,898,242 bp
  • C to A, chromosome 14 at 110,750,170 bp
  • A to G, chromosome 15 at 5,002,756 bp
  • T to C, chromosome 15 at 16,832,239 bp
  • C to T, chromosome 15 at 76,372,468 bp
  • A to T, chromosome 15 at 79,541,146 bp
  • G to A, chromosome 15 at 88,974,537 bp
  • A to T, chromosome 16 at 18,301,528 bp
  • A to G, chromosome 17 at 66,150,726 bp
  • A to G, chromosome 18 at 15,007,700 bp
  • A to C, chromosome 18 at 37,737,828 bp
  • C to T, chromosome 19 at 6,959,047 bp
  • T to C, chromosome 19 at 12,833,290 bp
  • G to A, chromosome 19 at 27,415,908 bp
  • C to T, chromosome 19 at 61,175,926 bp
  • A to G, chromosome X at 13,218,451 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4576 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041799-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.