Strain Name:
C57BL/6J-MtgxR4597Btlr/Mmmh
Stock Number:
041813-MU
Citation ID:
RRID:MMRRC_041813-MU
Other Names:
R4597 (G1), C57BL/6J-MtgxR4597Btlr
Major Collection:

Strain Information

Fga
Name: fibrinogen alpha chain
Synonyms: Fib, ENSMUSG00000059807
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 14161
HGNC: HGNC:3661
Homologene: 428
Drd4
Name: dopamine receptor D4
Synonyms: Drd-4, D4R
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 13491
HGNC: HGNC:3025
Homologene: 20215
Ank2
Name: ankyrin 2, brain
Synonyms: ankyrin B, Ank-2, Ankyrin-2, Ankyrin-B, Gm4392
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 109676
HGNC: HGNC:493
Cacna1h
Name: calcium channel, voltage-dependent, T type, alpha 1H subunit
Synonyms: Cav3.2, alpha13.2, T-type Cav3.2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 58226
HGNC: HGNC:1395
Homologene: 56913
Snx17
Name: sorting nexin 17
Synonyms: 5830447M19Rik, D5Ertd260e, b2b1625.1Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 266781
Homologene: 8838
Aopep
Name: aminopeptidase O
Synonyms: ApO, 2010111I01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 72061
HGNC: HGNC:1361
Homologene: 66273
Fkbp9
Name: FK506 binding protein 9
Synonyms: FKBP60, FKBP63
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 27055
HGNC: HGNC:3725
Homologene: 31434
Smg7
Name: SMG7 nonsense mediated mRNA decay factor
Synonyms: 9430023P16Rik, Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 226517
Homologene: 32235
Kif15
Name: kinesin family member 15
Synonyms: N-10 kinesin, HKLP2, 3930402I10Rik, 3110023M17Rik, Knsl7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 209737
VEGA: 9
Homologene: 23210
Arid2
Name: AT rich interactive domain 2 (ARID, RFX-like)
Synonyms: 4432409D24Rik, zipzap/p200, 1700124K17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 77044
Homologene: 14601
Cttnbp2nl
Name: CTTNBP2 N-terminal like
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 80281
Homologene: 36371
Carnmt1
Name: carnosine N-methyltransferase 1
Synonyms: 2410127L17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 67383
VEGA: 19
Homologene: 6806
Supt16
Name: SPT16, facilitates chromatin remodeling subunit
Synonyms: Cdc68, Fact140, Spt16, Supt16h
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 114741
VEGA: 14
Homologene: 5207
Mars1
Name: methionine-tRNA synthetase 1
Synonyms: MetRS, methionine tRNA ligase, methionyl-tRNA synthetase, Mars
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 216443
HGNC: HGNC:6898
Homologene: 90878
Sos1
Name: SOS Ras/Rac guanine nucleotide exchange factor 1
Synonyms: 4430401P03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 20662
VEGA: 17
Homologene: 4117
Gabbr1
Name: gamma-aminobutyric acid (GABA) B receptor, 1
Synonyms: GABAbR1, GABAB1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 54393
HGNC: HGNC:4070
Homologene: 1132
Casp12
Name: caspase 12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 12364
Homologene: 7654
Car9
Name: carbonic anhydrase 9
Synonyms: CAIX, MN/CA9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230099
HGNC: HGNC:1383
Homologene: 20325
Adh5
Name: alcohol dehydrogenase 5 (class III), chi polypeptide
Synonyms: Adh-5, Adh3, S-nitrosoglutathione reductase, GSNOR
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 11532
HGNC: HGNC:253
Homologene: 68076
Ncstn
Name: nicastrin
Synonyms: nicastrin, 9430068N19Rik, Nct, D1Dau13e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 59287
Homologene: 41029
Klhl2
Name: kelch-like 2, Mayven
Synonyms: ABP-KELCH, 6030411N21Rik, Mav, 8530402H02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 77113
HGNC: HGNC:6353
Homologene: 21416
Szt2
Name: SZT2 subunit of KICSTOR complex
Synonyms: seaizure threshold 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230676
Homologene: 49413
Itpr3
Name: inositol 1,4,5-triphosphate receptor 3
Synonyms: Itpr-3, Ip3r3, tf
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 16440
HGNC: HGNC:6182
Homologene: 1675
Clec10a
Name: C-type lectin domain family 10, member A
Synonyms: CD301a, Mgl1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 17312
Homologene: 7836
Qrfprl
Name: pyroglutamylated RFamide peptide receptor like
Synonyms: C130060K24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 243407
Homologene: 135976
Cdh23
Name: cadherin 23 (otocadherin)
Synonyms: 4930542A03Rik, USH1D, mdfw, ahl, bob, nmf112, nmf181, nmf252, sals
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 22295
Homologene: 11142
Pitpnm1
Name: phosphatidylinositol transfer protein, membrane-associated 1
Synonyms: DRES9, RdgB
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 18739
HGNC: HGNC:9003
Homologene: 3608
4931422A03Rik
Name: RIKEN cDNA 4931422A03 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 70986
Spata31d1c
Name: spermatogenesis associated 31 subfamily D, member 1C
Synonyms: Fam75d1c, 4932441B19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 238683
VEGA: 13
Mycbpap
Name: MYCBP associated protein
Synonyms: AMAP-1, 4932408B01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 104601
Homologene: 49982
Tnfsf8
Name: tumor necrosis factor (ligand) superfamily, member 8
Synonyms: Cd30L, CD153, CD30LG
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 21949
Homologene: 950
Itga9
Name: integrin alpha 9
Synonyms: 2610002H11Rik, D9Ertd428e, 6720458D17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 104099
HGNC: HGNC:6145
Homologene: 1664
Rin2
Name: Ras and Rab interactor 2
Synonyms: RASSF4, 4632403N06Rik, 2010003K16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 74030
Homologene: 32430
Myh2
Name: myosin, heavy polypeptide 2, skeletal muscle, adult
Synonyms: MyHC-IIa, MHC2A, Myhs-f1, Myhs-f, Myhsf1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 17882
HGNC: HGNC:7572
Homologene: 23019
Ltf
Name: lactotransferrin
Synonyms: Lf, lactoferrin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 17002
HGNC: HGNC:6720
Homologene: 1754
Or7e169
Name: olfactory receptor family 7 subfamily E member 169
Synonyms: GA_x6K02T2PVTD-13586614-13585661, MOR146-2, Olfr860
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 258521
Homologene: 134093
Il17a
Name: interleukin 17A
Synonyms: Ctla8, Ctla-8, Il17, IL-17A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 16171
HGNC: HGNC:5981
Homologene: 1651
Zfp770
Name: zinc finger protein 770
Synonyms: 6430601A21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 228491
Homologene: 82354
Klhl32
Name: kelch-like 32
Synonyms: LOC384000, D4Ertd389e, 6430524H05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 212390
Homologene: 14173
Col22a1
Name: collagen, type XXII, alpha 1
Synonyms: C80743, 2310067L16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 69700
Homologene: 43567
Frmpd1
Name: FERM and PDZ domain containing 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 666060
Homologene: 8939
Bcl3
Name: B cell leukemia/lymphoma 3
Synonyms: Bcl-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 12051
HGNC: HGNC:998
Homologene: 81738
Kif26b
Name: kinesin family member 26B
Synonyms: N-11 kinesin, D230039L06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 269152
Homologene: 18623
Lats1
Name: large tumor suppressor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 16798
VEGA: 10
HGNC: HGNC:6514
Homologene: 55843
Crocc
Name: ciliary rootlet coiled-coil, rootletin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230872
Homologene: 16811
Nipal4
Name: NIPA-like domain containing 4
Synonyms: 9530066K23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 214112
Homologene: 133769
Fam83d
Name: family with sequence similarity 83, member D
Synonyms: 2310007D09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 71878
Homologene: 23715
Ehbp1l1
Name: EH domain binding protein 1-like 1
Synonyms: G430002G23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 114601
VEGA: 19
Homologene: 136059
Sh2b2
Name: SH2B adaptor protein 2
Synonyms: Aps
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 23921
Homologene: 10309
Pira13
Name: paired-Ig-like receptor A13
Synonyms: ENSMUSG00000074419, Gm15448
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 100041146
HGNC: HGNC:6609
Homologene: 134028
Hoxa6
Name: homeobox A6
Synonyms: Hox-1.2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 15403
HGNC: HGNC:5107
Homologene: 7765
Vmn2r17
Name: vomeronasal 2, receptor 17
Synonyms: EG384221
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 384221
Homologene: 104825
Prpsap2
Name: phosphoribosyl pyrophosphate synthetase-associated protein 2
Synonyms: A230054F23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 212627
HGNC: HGNC:9467
Homologene: 2073
H60c
Name: histocompatibility 60c
Synonyms: Gm9491, 4632413I24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 670558
VEGA: 10
Homologene: 120804
H2-M10.2
Name: histocompatibility 2, M region locus 10.2
Synonyms: 4.7H
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 333715
Homologene: 117973
Krt1
Name: keratin 1
Synonyms: Krt2-1, Krt-2.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 16678
VEGA: 15
HGNC: HGNC:6412
Homologene: 38146
Dlgap1
Name: DLG associated protein 1
Synonyms: SAPAP1, DAP-1 beta, D17Bwg0511e, GKAP/SAPAP, Sapap1, 4933422O14Rik, Gkap
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 224997
HGNC: HGNC:2905
Homologene: 31258
Gm4353
Name: predicted gene 4353
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 100043313
VEGA: 7
Kcnc3
Name: potassium voltage gated channel, Shaw-related subfamily, member 3
Synonyms: Kv3.3, KShIIID, Kcr2-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 16504
HGNC: HGNC:6235
Homologene: 3650
H1f5
Name: H1.5 linker histone, cluster member
Synonyms: H1B, H1.5, H1s-3, H1f5, Hist1h1b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 56702
HGNC: HGNC:4719
Homologene: 135981
Gm4950
Name: predicted pseudogene 4950
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 240289
VEGA: 18
Pcdha9
Name: protocadherin alpha 9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 192161
Homologene: 130700
Vmn2r-ps36
Name: vomeronasal 2, receptor, pseudogene 36
Synonyms: EG665442
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 665442
Pex11g
Name: peroxisomal biogenesis factor 11 gamma
Synonyms: Pex11gamma, 1810049N02Rik, 1810022F11Rik, Pex11c
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 69129
Homologene: 12298
Aadacl2fm3
Name: AADACL2 family member 3
Synonyms: Gm8298
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 666803
Homologene: 86204
Gm27047
Name: predicted gene, 27047
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 102636949
Gm4925
Name: predicted gene 4925
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 237433
Homologene: 104935
Gm16710
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Gm16506
Name: predicted gene 16506
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 102634304
Homologene: 128452
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 20,730,993 bp
  • C to A, chromosome 1 at 152,840,301 bp
  • A to T, chromosome 1 at 172,068,256 bp
  • A to T, chromosome 1 at 178,916,793 bp
  • T to A, chromosome 2 at 104,026,155 bp
  • C to T, chromosome 2 at 114,196,770 bp
  • G to T, chromosome 2 at 145,860,905 bp
  • T to C, chromosome 2 at 158,785,222 bp
  • T to A, chromosome 3 at 59,876,793 bp
  • G to T, chromosome 3 at 83,031,235 bp
  • G to T, chromosome 3 at 105,005,875 bp
  • A to T, chromosome 3 at 126,988,151 bp
  • T to C, chromosome 3 at 138,445,357 bp
  • C to T, chromosome 4 at 24,629,339 bp
  • T to A, chromosome 4 at 43,509,138 bp
  • A to G, chromosome 4 at 45,274,441 bp
  • A to G, chromosome 4 at 63,837,100 bp
  • C to T, chromosome 4 at 118,372,681 bp
  • G to C, chromosome 4 at 141,019,777 bp
  • T to C, chromosome 5 at 31,198,513 bp
  • A to G, chromosome 5 at 109,429,562 bp
  • T to C, chromosome 5 at 136,231,762 bp
  • T to C, chromosome 6 at 52,208,407 bp
  • A to G, chromosome 6 at 56,832,382 bp
  • T to C, chromosome 6 at 65,447,424 bp
  • T to C, chromosome 6 at 130,630,336 bp
  • T to A, chromosome 7 at 3,822,155 bp
  • T to C, chromosome 7 at 7,430,137 bp
  • T to G, chromosome 7 at 19,812,503 bp
  • T to A, chromosome 7 at 44,595,816 bp
  • T to A, chromosome 7 at 116,083,612 bp
  • T to C, chromosome 7 at 141,294,479 bp
  • A to G, chromosome 8 at 3,464,043 bp
  • C to A, chromosome 8 at 64,754,387 bp
  • C to T, chromosome 9 at 5,348,941 bp
  • T to C, chromosome 9 at 19,845,691 bp
  • G to A, chromosome 9 at 111,022,933 bp
  • T to C, chromosome 9 at 118,843,514 bp
  • A to G, chromosome 9 at 122,993,849 bp
  • A to T, chromosome 10 at 3,259,968 bp
  • T to C, chromosome 10 at 7,691,746 bp
  • A to G, chromosome 10 at 60,409,044 bp
  • A to T, chromosome 10 at 88,730,746 bp
  • A to G, chromosome 10 at 127,300,453 bp
  • A to G, chromosome 11 at 46,151,329 bp
  • A to T, chromosome 11 at 61,748,949 bp
  • A to C, chromosome 11 at 67,189,418 bp
  • A to T, chromosome 11 at 70,169,980 bp
  • C to T, chromosome 11 at 94,503,177 bp
  • A to G, chromosome 12 at 115,802,656 bp
  • A to T, chromosome 13 at 21,780,511 bp
  • T to C, chromosome 13 at 63,068,092 bp
  • T to A, chromosome 13 at 65,035,613 bp
  • A to G, chromosome 14 at 43,725,115 bp
  • C to T, chromosome 14 at 52,173,589 bp
  • G to T, chromosome 15 at 71,964,662 bp
  • A to G, chromosome 15 at 96,370,856 bp
  • C to T, chromosome 15 at 101,847,628 bp
  • C to T, chromosome 17 at 25,433,124 bp
  • C to T, chromosome 17 at 27,093,283 bp
  • T to C, chromosome 17 at 36,285,393 bp
  • A to G, chromosome 17 at 37,056,899 bp
  • T to C, chromosome 17 at 70,516,600 bp
  • T to C, chromosome 17 at 80,433,826 bp
  • C to G, chromosome 18 at 36,931,906 bp
  • A to T, chromosome 18 at 51,865,793 bp
  • T to C, chromosome 19 at 4,105,419 bp
  • T to C, chromosome 19 at 5,717,927 bp
  • G to T, chromosome 19 at 18,671,087 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4597 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041813-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.