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Strain Name:
C57BL/6J-MtgxR4607Btlr/Mmmh
Stock Number:
041818-MU
Citation ID:
RRID:MMRRC_041818-MU
Other Names:
R4607 (G1), C57BL/6J-MtgxR4607Btlr
Major Collection:

Strain Information

Chrna9
Name: cholinergic receptor, nicotinic, alpha polypeptide 9
Synonyms: Acra9, 2410015I05Rik, Gm8311
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231252
Homologene: 9729
Pcdh9
Name: protocadherin 9
Synonyms: C630029H24Rik, 1500001L12Rik, LOC382930, A730003J17Rik, C530050I23Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 211712
HGNC: HGNC:8661
Homologene: 10698
Slit1
Name: slit guidance ligand 1
Synonyms: Slil1
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 20562
Homologene: 2302
Tlk2
Name: tousled-like kinase 2 (Arabidopsis)
Synonyms: protein kinase U-alpha, PKUalpha, 4933403M19Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 24086
Homologene: 4993
Uba2
Name: ubiquitin-like modifier activating enzyme 2
Synonyms: Sumo-1 activating enzyme subunit 2, UBA2, anthracycline-associated resistance, Arx, SAE2, Uble1b
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 50995
Homologene: 4018
Eif4g3
Name: eukaryotic translation initiation factor 4 gamma, 3
Synonyms: eIF4GII, 1500002J22Rik, 4930523M17Rik, G1-419-52, repro8
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230861
HGNC: HGNC:3298
Homologene: 2789
Ipo11
Name: importin 11
Synonyms: E330021B14Rik, 1700081H05Rik, 2510001A17Rik, Ranbp11
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 76582
Homologene: 7089
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 2 at 37,202,082 bp
  • T to A, chromosome 2 at 53,103,716 bp
  • G to A, chromosome 2 at 69,183,899 bp
  • G to T, chromosome 2 at 89,074,312 bp
  • T to A, chromosome 2 at 121,372,945 bp
  • T to A, chromosome 2 at 174,423,162 bp
  • C to A, chromosome 2 at 181,588,774 bp
  • C to T, chromosome 3 at 65,946,710 bp
  • T to C, chromosome 3 at 79,686,889 bp
  • G to A, chromosome 3 at 155,086,696 bp
  • C to A, chromosome 4 at 56,741,114 bp
  • G to T, chromosome 4 at 138,126,458 bp
  • A to C, chromosome 5 at 49,970,739 bp
  • A to G, chromosome 5 at 65,976,735 bp
  • G to A, chromosome 5 at 127,551,571 bp
  • T to C, chromosome 6 at 41,218,401 bp
  • T to A, chromosome 6 at 78,467,503 bp
  • T to A, chromosome 6 at 116,328,234 bp
  • T to A, chromosome 7 at 4,144,797 bp
  • T to A, chromosome 7 at 30,977,745 bp
  • T to C, chromosome 7 at 34,154,596 bp
  • C to A, chromosome 7 at 43,713,860 bp
  • G to A, chromosome 7 at 80,387,211 bp
  • A to T, chromosome 7 at 86,394,510 bp
  • A to G, chromosome 7 at 100,938,304 bp
  • T to A, chromosome 7 at 125,958,549 bp
  • A to G, chromosome 7 at 140,574,641 bp
  • T to C, chromosome 7 at 140,943,433 bp
  • G to A, chromosome 8 at 68,810,619 bp
  • C to T, chromosome 8 at 79,997,563 bp
  • T to A, chromosome 9 at 52,068,837 bp
  • T to A, chromosome 10 at 106,967,029 bp
  • T to G, chromosome 10 at 111,300,820 bp
  • A to G, chromosome 10 at 111,301,134 bp
  • G to A, chromosome 11 at 23,020,710 bp
  • G to A, chromosome 11 at 36,171,381 bp
  • A to G, chromosome 11 at 70,606,067 bp
  • T to A, chromosome 11 at 105,255,018 bp
  • C to T, chromosome 12 at 112,680,542 bp
  • G to A, chromosome 13 at 50,245,506 bp
  • A to G, chromosome 13 at 106,900,811 bp
  • A to G, chromosome 14 at 47,413,018 bp
  • T to A, chromosome 14 at 53,560,054 bp
  • T to A, chromosome 14 at 93,015,573 bp
  • G to T, chromosome 15 at 43,867,158 bp
  • T to C, chromosome 16 at 76,293,032 bp
  • A to T, chromosome 17 at 37,170,282 bp
  • G to A, chromosome 17 at 74,258,461 bp
  • C to T, chromosome 18 at 20,471,245 bp
  • T to C, chromosome 18 at 32,199,977 bp
  • A to G, chromosome 18 at 37,708,618 bp
  • C to T, chromosome 18 at 63,777,580 bp
  • G to T, chromosome 19 at 10,229,067 bp
  • A to G, chromosome 19 at 16,533,711 bp
  • A to T, chromosome 19 at 20,621,687 bp
  • A to G, chromosome 19 at 37,174,839 bp
  • C to A, chromosome 19 at 41,616,793 bp
  • C to T, chromosome 19 at 44,063,035 bp
  • C to T, chromosome Y at 1,131,134 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4607 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041818-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.