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Strain Name:
C57BL/6J-MtgxR4625Btlr/Mmmh
Stock Number:
041890-MU
Citation ID:
RRID:MMRRC_041890-MU
Other Names:
R4625 (G1), C57BL/6J-MtgxR4625Btlr
Major Collection:

Strain Information

Kera
Name: keratocan
Synonyms: SLRR2B, CNA2
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 16545
VEGA: 10
HGNC: HGNC:6309
Homologene: 5106
Ptch1
Name: patched 1
Synonyms: Ptc, Patched 1, Ptc1, A230106A15Rik, wig
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 19206
HGNC: HGNC:9585
Homologene: 223
Aopep
Name: aminopeptidase O
Synonyms: ApO, 2010111I01Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 72061
HGNC: HGNC:1361
Homologene: 66273
Ttc3
Name: tetratricopeptide repeat domain 3
Synonyms: TPRD, D16Ium21, 2610202A04Rik, D16Ium21e
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 22129
Homologene: 2487
Slc25a26
Name: solute carrier family 25 (mitochondrial carrier, phosphate carrier), member 26
Synonyms: 4930433D19Rik, 4933433F13Rik, D6Bwg0781e
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 67582
Homologene: 6834
Agrn
Name: agrin
Synonyms: NMF380, Agrin, nmf380
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11603
HGNC: HGNC:329
Homologene: 27907
Lin9
Name: lin-9 DREAM MuvB core complex component
Synonyms: 2700022J23Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 72568
Homologene: 35252
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to C, chromosome 1 at 85,577,329 bp
  • A to T, chromosome 1 at 88,254,965 bp
  • A to T, chromosome 1 at 93,042,659 bp
  • A to C, chromosome 1 at 97,459,626 bp
  • A to G, chromosome 1 at 153,242,696 bp
  • A to T, chromosome 1 at 158,580,294 bp
  • T to A, chromosome 1 at 180,689,280 bp
  • A to G, chromosome 2 at 36,688,071 bp
  • A to G, chromosome 2 at 160,969,492 bp
  • G to A, chromosome 2 at 163,314,416 bp
  • A to G, chromosome 2 at 165,112,606 bp
  • A to G, chromosome 2 at 167,188,233 bp
  • C to T, chromosome 3 at 102,778,145 bp
  • C to T, chromosome 3 at 105,658,766 bp
  • A to C, chromosome 3 at 106,128,940 bp
  • A to G, chromosome 4 at 55,530,370 bp
  • T to G, chromosome 4 at 58,072,698 bp
  • G to C, chromosome 4 at 156,171,404 bp
  • G to T, chromosome 5 at 22,548,005 bp
  • T to A, chromosome 5 at 34,829,785 bp
  • G to T, chromosome 5 at 90,571,752 bp
  • C to T, chromosome 5 at 114,124,226 bp
  • A to C, chromosome 5 at 145,044,883 bp
  • C to T, chromosome 6 at 88,836,287 bp
  • G to T, chromosome 6 at 94,507,652 bp
  • G to A, chromosome 7 at 30,038,595 bp
  • C to A, chromosome 7 at 47,052,865 bp
  • T to A, chromosome 7 at 79,601,604 bp
  • A to G, chromosome 7 at 97,347,575 bp
  • T to A, chromosome 7 at 101,901,032 bp
  • T to C, chromosome 7 at 108,367,688 bp
  • A to T, chromosome 7 at 131,393,753 bp
  • T to A, chromosome 8 at 80,710,563 bp
  • T to C, chromosome 8 at 111,276,358 bp
  • A to T, chromosome 9 at 8,677,962 bp
  • A to G, chromosome 9 at 21,929,466 bp
  • A to T, chromosome 9 at 39,258,318 bp
  • A to T, chromosome 9 at 54,404,120 bp
  • A to C, chromosome 9 at 64,235,798 bp
  • G to A, chromosome 9 at 104,036,159 bp
  • T to C, chromosome 9 at 121,915,585 bp
  • A to G, chromosome 10 at 97,609,631 bp
  • A to T, chromosome 10 at 105,303,650 bp
  • T to C, chromosome 11 at 6,512,240 bp
  • A to G, chromosome 11 at 59,067,258 bp
  • A to G, chromosome 11 at 69,463,661 bp
  • G to A, chromosome 11 at 84,911,490 bp
  • C to A, chromosome 12 at 76,587,326 bp
  • G to A, chromosome 13 at 34,932,465 bp
  • A to G, chromosome 13 at 49,557,425 bp
  • A to G, chromosome 13 at 58,409,136 bp
  • T to C, chromosome 13 at 63,068,092 bp
  • T to C, chromosome 13 at 63,523,164 bp
  • T to A, chromosome 13 at 65,152,860 bp
  • C to A, chromosome 13 at 100,655,673 bp
  • T to A, chromosome 13 at 104,249,393 bp
  • A to G, chromosome 13 at 118,386,714 bp
  • G to T, chromosome 13 at 120,035,229 bp
  • T to C, chromosome 14 at 13,158,798 bp
  • A to G, chromosome 14 at 33,956,099 bp
  • T to C, chromosome 15 at 9,647,438 bp
  • A to C, chromosome 15 at 22,714,042 bp
  • A to T, chromosome 15 at 100,313,058 bp
  • C to T, chromosome 15 at 101,846,187 bp
  • A to T, chromosome 16 at 69,858,754 bp
  • T to A, chromosome 16 at 94,388,272 bp
  • A to T, chromosome 17 at 20,048,181 bp
  • A to G, chromosome 17 at 34,379,751 bp
  • T to A, chromosome 17 at 55,964,598 bp
  • G to T, chromosome 17 at 65,929,551 bp
  • A to T, chromosome 17 at 80,050,862 bp
  • T to C, chromosome 17 at 88,985,720 bp
  • A to T, chromosome 19 at 29,810,863 bp
  • A to G, chromosome 19 at 43,803,739 bp
  • A to T, chromosome 19 at 45,029,762 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4625 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041890-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.