Strain Name:
C57BL/6J-MtgxR4645Btlr/Mmmh
Stock Number:
041906-MU
Citation ID:
RRID:MMRRC_041906-MU
Other Names:
R4645 (G1), C57BL/6J-MtgxR4645Btlr
Major Collection:

Strain Information

Sim1
Name: single-minded family bHLH transcription factor 1
Synonyms: bHLHe14
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 20464
VEGA: 10
Homologene: 3715
Pomt1
Name: protein-O-mannosyltransferase 1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 99011
HGNC: HGNC:9202
Homologene: 68548
Arpp21
Name: cyclic AMP-regulated phosphoprotein, 21
Synonyms: Tarpp, ARPP-21, 0710001E13Rik, R3hdm3, D9Bwg1012e
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 74100
Homologene: 32306
Mok
Name: MOK protein kinase
Synonyms: MAPK/MAK/MRK/ overlapping kinase, MOK, Stk30, Rage
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 26448
HGNC: HGNC:9833
Homologene: 8062
Zmym2
Name: zinc finger, MYM-type 2
Synonyms: RAMP, MYM, Zfp198, FIM, SCLL
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 76007
VEGA: 14
Homologene: 12631
Ccdc14
Name: coiled-coil domain containing 14
Synonyms: G630039H03Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 239839
VEGA: 16
Homologene: 32549
Lrp6
Name: low density lipoprotein receptor-related protein 6
Synonyms: skam26Jus, skax26, Cd, ska26
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 16974
HGNC: HGNC:6698
Homologene: 1747
Slc8a2
Name: solute carrier family 8 (sodium/calcium exchanger), member 2
Synonyms: Ncx2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 110891
Homologene: 27358
Gas2l3
Name: growth arrest-specific 2 like 3
Synonyms: LOC237436, 8430435B07Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 237436
VEGA: 10
Homologene: 18386
Immt
Name: inner membrane protein, mitochondrial
Synonyms: P87, 1700082C19Rik, D830041H16Rik, P89, P87/89, mitofilin, HMP, Micos60
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 76614
HGNC: HGNC:6047
Homologene: 38234
Traf3
Name: TNF receptor-associated factor 3
Synonyms: CAP-1, CRAF1, LAP1, CD40bp
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 22031
Homologene: 7981
Pyroxd1
Name: pyridine nucleotide-disulphide oxidoreductase domain 1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 232491
Homologene: 11758
Glud1
Name: glutamate dehydrogenase 1
Synonyms: Gdh-X, Glud
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 14661
Homologene: 55885
Thap1
Name: THAP domain containing, apoptosis associated protein 1
Synonyms: 4833431A01Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 73754
Homologene: 10005
Tenm4
Name: teneurin transmembrane protein 4
Synonyms: Doc4, l7Rn3, ELM2, Odz4, l(7)-3Rn, Ten-m4
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 23966
Homologene: 8034
Sympk
Name: symplekin
Synonyms: 1500016F02Rik, 4632415H16Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 68188
Homologene: 37969
Zfyve28
Name: zinc finger, FYVE domain containing 28
Synonyms: 9630058O20Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231125
Homologene: 15119
Klhl6
Name: kelch-like 6
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 239743
Homologene: 15603
Adgrf5
Name: adhesion G protein-coupled receptor F5
Synonyms: Gpr116, 8430401C09Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 224792
VEGA: 17
Homologene: 9065
Ttn
Name: titin
Synonyms: connectin, shru, D330041I19Rik, 2310057K23Rik, mdm, D830007G01Rik, 2310074I15Rik, 1100001C23Rik, 2310036G12Rik, L56
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 22138
Homologene: 130650
Celsr1
Name: cadherin, EGF LAG seven-pass G-type receptor 1
Synonyms: Scy, Crsh, Adgrc1, crash
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 12614
VEGA: 15
HGNC: HGNC:1850
Homologene: 7665
Fam135b
Name: family with sequence similarity 135, member B
Synonyms: A830008O07Rik, 1700010C24Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 70363
VEGA: 15
Homologene: 66605
Celsr2
Name: cadherin, EGF LAG seven-pass G-type receptor 2
Synonyms: flamingo, mfmi1, EGFL2, Adgrc2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 53883
HGNC: HGNC:3231
Homologene: 1078
Agfg2
Name: ArfGAP with FG repeats 2
Synonyms: A630095P14Rik, Hrbl
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231801
HGNC: HGNC:5177
Homologene: 4430
Spaca6
Name: sperm acrosome associated 6
Synonyms: 4930546H06Rik, B230206P06Rik, Ncrna00085
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 75202
Homologene: 53443
Ap4b1
Name: adaptor-related protein complex AP-4, beta 1
Synonyms: AP-4 beta-4, 1810038H16Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 67489
HGNC: HGNC:572
Homologene: 38203
Unc79
Name: unc-79 homolog
Synonyms: 9030205A07Rik, Mlca3
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 217843
Homologene: 41397
Mmp12
Name: matrix metallopeptidase 12
Synonyms: macrophage elastase, MMP12, Mmel
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 17381
HGNC: HGNC:7158
Homologene: 20547
Shank2
Name: SH3 and multiple ankyrin repeat domains 2
Synonyms: ProSAP1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 210274
Homologene: 105965
Lrit2
Name: leucine-rich repeat, immunoglobulin-like and transmembrane domains 2
Synonyms: A930010E21Rik, Lrrc22
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 239038
VEGA: 14
Homologene: 18292
Vmn2r74
Name: vomeronasal 2, receptor 74
Synonyms: EG546980
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 546980
Homologene: 115466
Adprs
Name: ADP-ribosylserine hydrolase
Synonyms: Arh3, Adprhl2
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 100206
Homologene: 9863
Iqsec1
Name: IQ motif and Sec7 domain 1
Synonyms: cI-43, D6Ertd349e, BRAG2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 232227
Homologene: 82429
Zscan4e
Name: zinc finger and SCAN domain containing 4E
Synonyms: EG665848
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 665848
Homologene: 85986
Cntnap3
Name: contactin associated protein-like 3
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 238680
VEGA: 13
Homologene: 129602
Ccr9
Name: C-C motif chemokine receptor 9
Synonyms: Cmkbr10, GPR-9-6
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 12769
HGNC: HGNC:1610
Homologene: 22546
Lrriq4
Name: leucine-rich repeats and IQ motif containing 4
Synonyms: 4930558O21Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 68307
Homologene: 12260
Hsp90b1
Name: heat shock protein 90, beta (Grp94), member 1
Synonyms: tumor rejection antigen (gp96) 1, gp96, Targ2, Tra1, endoplasmin, Tra-1, GRP94, ERp99, 90 kDa
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 22027
Homologene: 2476
Lztr1
Name: leucine-zipper-like transcriptional regulator, 1
Synonyms: TCFL2, 1200003E21Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 66863
HGNC: HGNC:6742
Homologene: 4925
Abcc10
Name: ATP-binding cassette, sub-family C member 10
Synonyms: Mrp7
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 224814
HGNC: HGNC:52
Homologene: 58616
Fam184a
Name: family with sequence similarity 184, member A
Synonyms: 4930589M24Rik, 4930438C08Rik, 3110012E06Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 75906
Homologene: 11600
Vmn2r50
Name: vomeronasal 2, receptor 50
Synonyms: EG434117
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 434117
Homologene: 113703
Zswim2
Name: zinc finger SWIM-type containing 2
Synonyms: 1700025P14Rik, 4933437F18Rik, MEX
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 71861
Homologene: 32689
Mpi
Name: mannose phosphate isomerase
Synonyms: 1110002E17Rik, Mpi-1, Mpi1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 110119
HGNC: HGNC:7216
Homologene: 1825
Ly96
Name: lymphocyte antigen 96
Synonyms: myeloid differentiation factor-2, ESOP-1, MD2, MD-2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 17087
Homologene: 9109
Or4c101
Name: olfactory receptor family 4 subfamily C member 101
Synonyms: GA_x6K02T2Q125-50046879-50047784, Olfr1188, MOR238-2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258921
Erlin1
Name: ER lipid raft associated 1
Synonyms: Spfh1, Keo4, 2810439N09Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 226144
Homologene: 4716
Ptpn20
Name: protein tyrosine phosphatase, non-receptor type 20
Synonyms: typ
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 19256
VEGA: 14
Homologene: 74905
Gm17415
Name: predicted gene, 17415
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 108167670
AY702103
Name: cDNA sequence AY702103
Synonyms: STDP2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 446210
Tysnd1
Name: trypsin domain containing 1
Synonyms: 1300019N10Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 71767
VEGA: 10
Homologene: 87946
Lbhd1
Name: LBH domain containing 1
Synonyms: Gm21743
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 102308570
Homologene: 75203
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to T, chromosome 1 at 16,691,716 bp
  • A to G, chromosome 1 at 93,422,120 bp
  • G to T, chromosome 2 at 32,242,876 bp
  • T to C, chromosome 2 at 76,748,336 bp
  • G to T, chromosome 2 at 83,915,547 bp
  • T to C, chromosome 2 at 88,560,378 bp
  • A to G, chromosome 3 at 30,650,743 bp
  • A to G, chromosome 3 at 103,821,449 bp
  • C to A, chromosome 3 at 108,395,969 bp
  • C to A, chromosome 4 at 126,316,576 bp
  • T to G, chromosome 5 at 34,222,443 bp
  • T to A, chromosome 5 at 137,684,592 bp
  • T to C, chromosome 6 at 71,856,939 bp
  • T to G, chromosome 6 at 90,668,013 bp
  • T to C, chromosome 6 at 134,484,250 bp
  • C to G, chromosome 6 at 142,354,741 bp
  • T to A, chromosome 7 at 10,037,235 bp
  • T to C, chromosome 7 at 11,307,075 bp
  • C to T, chromosome 7 at 16,134,239 bp
  • G to T, chromosome 7 at 19,043,460 bp
  • C to T, chromosome 7 at 85,957,109 bp
  • G to T, chromosome 7 at 96,895,742 bp
  • A to G, chromosome 7 at 144,410,422 bp
  • A to G, chromosome 8 at 26,162,569 bp
  • A to G, chromosome 9 at 7,347,515 bp
  • T to C, chromosome 9 at 57,550,757 bp
  • A to G, chromosome 9 at 112,176,951 bp
  • G to T, chromosome 9 at 123,779,593 bp
  • A to T, chromosome 10 at 50,983,997 bp
  • G to A, chromosome 10 at 53,698,498 bp
  • G to T, chromosome 10 at 61,696,183 bp
  • A to G, chromosome 10 at 86,695,835 bp
  • CACTCGTCATACT to CACT, chromosome 10 at 89,430,958 bp
  • T to C, chromosome 12 at 103,112,822 bp
  • A to T, chromosome 12 at 110,808,439 bp
  • T to C, chromosome 12 at 111,261,966 bp
  • A to G, chromosome 13 at 64,778,788 bp
  • G to A, chromosome 14 at 33,631,212 bp
  • T to C, chromosome 14 at 34,311,106 bp
  • C to T, chromosome 14 at 37,072,475 bp
  • T to C, chromosome 14 at 56,928,307 bp
  • T to C, chromosome 15 at 71,462,340 bp
  • A to T, chromosome 15 at 85,916,756 bp
  • C to T, chromosome 16 at 17,524,091 bp
  • A to T, chromosome 16 at 19,947,147 bp
  • T to C, chromosome 16 at 34,721,740 bp
  • A to T, chromosome 17 at 17,836,045 bp
  • A to G, chromosome 17 at 43,437,525 bp
  • T to C, chromosome 17 at 46,324,774 bp
  • G to T, chromosome 17 at 50,239,913 bp
  • T to G, chromosome 19 at 8,884,088 bp
  • T to C, chromosome 19 at 44,069,320 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4645 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041906-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.