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Strain Name:
C57BL/6J-MtgxR4649Btlr/Mmmh
Stock Number:
041910-MU
Citation ID:
RRID:MMRRC_041910-MU
Other Names:
R4649 (G1), C57BL/6J-MtgxR4649Btlr
Major Collection:

Strain Information

Agpat5
Name: 1-acylglycerol-3-phosphate O-acyltransferase 5
Synonyms: 1110013A05Rik, D8Ertd319e
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 52123
Homologene: 10153
Adcy10
Name: adenylate cyclase 10
Synonyms: soluble adenylyl cyclase, sAC, 4930431D04Rik, Sacy
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 271639
Homologene: 10188
Mga
Name: MAX gene associated
Synonyms: Mga, Mad5, C130042M01Rik, D030062C11Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 29808
Homologene: 49351
Itsn1
Name: intersectin 1 (SH3 domain protein 1A)
Synonyms: Ese1, EHSH1, Sh3p17, Eh domain, SH3 domain regulator of endocytosis 1, Intersectin-L
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 16443
HGNC: HGNC:6183
Homologene: 2277
Nudt5
Name: nudix hydrolase 5
Synonyms: nudix (nucleoside diphosphate linked moiety X)-type motif 5
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 53893
HGNC: HGNC:8052
Homologene: 5388
Znfx1
Name: zinc finger, NFX1-type containing 1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 98999
Homologene: 10877
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 73,953,771 bp
  • G to A, chromosome 1 at 87,125,810 bp
  • T to C, chromosome 1 at 128,184,444 bp
  • T to G, chromosome 1 at 153,228,777 bp
  • A to T, chromosome 1 at 153,817,582 bp
  • G to A, chromosome 1 at 165,504,049 bp
  • A to G, chromosome 1 at 175,687,396 bp
  • G to A, chromosome 2 at 5,856,065 bp
  • T to C, chromosome 2 at 11,279,522 bp
  • A to G, chromosome 2 at 26,339,742 bp
  • G to A, chromosome 2 at 68,606,215 bp
  • C to A, chromosome 2 at 89,789,293 bp
  • T to A, chromosome 2 at 90,139,088 bp
  • T to C, chromosome 2 at 119,431,008 bp
  • T to A, chromosome 2 at 119,941,493 bp
  • G to T, chromosome 2 at 167,056,356 bp
  • G to A, chromosome 2 at 173,207,815 bp
  • A to G, chromosome 2 at 181,797,414 bp
  • T to A, chromosome 3 at 30,810,534 bp
  • T to C, chromosome 3 at 95,888,265 bp
  • G to A, chromosome 3 at 108,904,092 bp
  • A to T, chromosome 3 at 113,151,210 bp
  • A to G, chromosome 3 at 146,048,167 bp
  • G to A, chromosome 3 at 152,279,005 bp
  • C to A, chromosome 4 at 3,738,850 bp
  • T to A, chromosome 4 at 55,009,349 bp
  • T to C, chromosome 4 at 95,967,396 bp
  • A to G, chromosome 4 at 103,213,229 bp
  • T to A, chromosome 4 at 125,650,485 bp
  • G to A, chromosome 4 at 128,546,073 bp
  • A to C, chromosome 4 at 152,312,155 bp
  • T to C, chromosome 4 at 156,166,571 bp
  • A to T, chromosome 5 at 21,226,311 bp
  • G to T, chromosome 5 at 36,071,351 bp
  • T to C, chromosome 5 at 88,492,968 bp
  • C to T, chromosome 5 at 97,053,111 bp
  • C to T, chromosome 5 at 105,041,941 bp
  • T to C, chromosome 5 at 114,946,129 bp
  • A to G, chromosome 6 at 70,956,976 bp
  • T to A, chromosome 6 at 120,224,620 bp
  • G to T, chromosome 7 at 23,418,178 bp
  • TCTCCTCCTCCTCCTCCTCCTCCTCC to TCTCCTCCTCCTCCTCCTCCTCC, chromosome 7 at 30,586,358 bp
  • A to G, chromosome 7 at 41,158,772 bp
  • A to G, chromosome 7 at 42,903,690 bp
  • T to C, chromosome 7 at 66,273,053 bp
  • C to A, chromosome 7 at 85,221,535 bp
  • C to A, chromosome 7 at 120,047,698 bp
  • T to C, chromosome 7 at 126,291,129 bp
  • A to G, chromosome 7 at 126,414,492 bp
  • A to G, chromosome 7 at 128,189,531 bp
  • A to G, chromosome 7 at 141,297,573 bp
  • A to G, chromosome 8 at 18,879,652 bp
  • G to A, chromosome 8 at 71,570,764 bp
  • A to T, chromosome 8 at 78,333,579 bp
  • A to G, chromosome 8 at 88,806,691 bp
  • A to G, chromosome 8 at 126,447,195 bp
  • G to T, chromosome 9 at 20,470,627 bp
  • A to C, chromosome 9 at 24,482,054 bp
  • T to A, chromosome 9 at 55,221,049 bp
  • T to A, chromosome 9 at 56,886,865 bp
  • A to T, chromosome 9 at 79,639,794 bp
  • T to C, chromosome 9 at 86,679,852 bp
  • A to G, chromosome 9 at 96,471,830 bp
  • C to A, chromosome 9 at 97,362,945 bp
  • A to G, chromosome 10 at 3,956,985 bp
  • T to A, chromosome 10 at 6,919,733 bp
  • C to A, chromosome 10 at 14,468,827 bp
  • T to A, chromosome 10 at 52,129,668 bp
  • A to T, chromosome 10 at 80,669,339 bp
  • A to T, chromosome 10 at 103,009,332 bp
  • C to A, chromosome 10 at 127,710,217 bp
  • T to A, chromosome 10 at 128,397,267 bp
  • A to T, chromosome 12 at 36,123,981 bp
  • G to T, chromosome 12 at 71,164,546 bp
  • A to T, chromosome 12 at 71,164,547 bp
  • G to A, chromosome 12 at 101,541,512 bp
  • T to A, chromosome 13 at 12,508,423 bp
  • A to T, chromosome 13 at 23,782,969 bp
  • A to T, chromosome 13 at 34,899,971 bp
  • C to T, chromosome 13 at 49,945,215 bp
  • G to A, chromosome 13 at 54,379,548 bp
  • G to A, chromosome 13 at 56,887,554 bp
  • A to T, chromosome 13 at 59,528,399 bp
  • A to G, chromosome 13 at 74,746,014 bp
  • A to G, chromosome 13 at 85,221,428 bp
  • C to T, chromosome 14 at 30,095,408 bp
  • T to A, chromosome 14 at 32,102,409 bp
  • A to T, chromosome 14 at 47,529,628 bp
  • C to A, chromosome 14 at 70,315,793 bp
  • G to A, chromosome 14 at 80,021,307 bp
  • T to C, chromosome 14 at 121,478,092 bp
  • G to A, chromosome 15 at 39,138,242 bp
  • A to G, chromosome 15 at 68,184,476 bp
  • T to C, chromosome 15 at 78,255,989 bp
  • T to A, chromosome 16 at 33,196,082 bp
  • T to A, chromosome 16 at 91,841,588 bp
  • T to A, chromosome 17 at 24,904,752 bp
  • C to T, chromosome 17 at 28,778,487 bp
  • T to A, chromosome 17 at 33,002,925 bp
  • A to G, chromosome 17 at 34,472,628 bp
  • T to C, chromosome 17 at 35,832,544 bp
  • T to C, chromosome 17 at 36,057,876 bp
  • T to C, chromosome 17 at 84,575,263 bp
  • A to G, chromosome 17 at 88,549,513 bp
  • T to A, chromosome 18 at 20,602,245 bp
  • C to T, chromosome 18 at 49,878,631 bp
  • T to C, chromosome 18 at 50,568,188 bp
  • G to A, chromosome 18 at 60,916,651 bp
  • A to G, chromosome 18 at 60,917,098 bp
  • A to T, chromosome 18 at 82,597,659 bp
  • A to G, chromosome 19 at 29,810,321 bp
  • G to C, chromosome X at 37,245,309 bp
  • A to T, chromosome X at 37,245,362 bp
  • T to C, chromosome X at 167,352,292 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4649 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041910-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.