Strain Name:
C57BL/6J-MtgxR4660Btlr/Mmmh
Stock Number:
041920-MU
Citation ID:
RRID:MMRRC_041920-MU
Other Names:
R4660 (G1), C57BL/6J-MtgxR4660Btlr
Major Collection:

Gene Information

Gene Symbol: Itga8 [MGI:109442] (Mus musculus (mouse))
Name: integrin alpha 8
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: Sema3c [MGI:107557] (Mus musculus (mouse))
Name: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3C
Synonyms: Semae, 1110036B02Rik
Chromosome: 5
Alteration at locus: Chemically Induced
Gene Symbol: Tnf [MGI:104798] (Mus musculus (mouse))
Name: tumor necrosis factor
Synonyms: Tnfa, TNFalpha, TNF-alpha, Tnfsf1a, tumor necrosis factor-alpha, DIF, TNF alpha
Chromosome: 17
Alteration at locus: Chemically Induced
Gene Symbol: Ltbp3 [MGI:1101355] (Mus musculus (mouse))
Name: latent transforming growth factor beta binding protein 3
Synonyms: Ltbp2
Chromosome: 19
Alteration at locus: Chemically Induced
Gene Symbol: Mga [MGI:1352483] (Mus musculus (mouse))
Name: MAX gene associated
Synonyms: Mad5, C130042M01Rik, Mga, D030062C11Rik
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: Msantd3 [MGI:1913915] (Mus musculus (mouse))
Name: Myb/SANT-like DNA-binding domain containing 3
Synonyms: 2410046L14Rik, 2810429P03Rik, 5730528L13Rik
Chromosome: 4
Alteration at locus: Chemically Induced
Gene Symbol: Eif2s2 [MGI:1914454] (Mus musculus (mouse))
Name: eukaryotic translation initiation factor 2, subunit 2 (beta)
Synonyms: 2810026E11Rik, EIF2, EIF2B, 38kDa, D2Ertd303e
Chromosome: 2
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 37,946,861 bp
  • A to T, chromosome 1 at 46,289,536 bp
  • T to C, chromosome 1 at 51,301,351 bp
  • T to C, chromosome 1 at 78,498,478 bp
  • A to T, chromosome 1 at 151,407,597 bp
  • G to A, chromosome 1 at 162,680,895 bp
  • C to T, chromosome 1 at 164,306,493 bp
  • A to G, chromosome 2 at 5,873,693 bp
  • A to G, chromosome 2 at 12,265,258 bp
  • T to A, chromosome 2 at 52,255,588 bp
  • A to T, chromosome 2 at 94,364,429 bp
  • T to A, chromosome 2 at 119,938,623 bp
  • T to C, chromosome 2 at 131,561,142 bp
  • G to A, chromosome 2 at 154,888,269 bp
  • C to T, chromosome 2 at 162,997,843 bp
  • T to C, chromosome 3 at 32,520,530 bp
  • T to A, chromosome 3 at 40,821,937 bp
  • C to T, chromosome 3 at 59,618,703 bp
  • T to G, chromosome 3 at 88,120,176 bp
  • T to A, chromosome 3 at 94,403,365 bp
  • T to A, chromosome 3 at 106,731,795 bp
  • T to A, chromosome 3 at 108,572,977 bp
  • A to T, chromosome 3 at 133,326,684 bp
  • A to G, chromosome 3 at 152,287,518 bp
  • T to C, chromosome 4 at 11,513,505 bp
  • C to T, chromosome 4 at 32,562,777 bp
  • A to G, chromosome 4 at 48,552,536 bp
  • C to T, chromosome 4 at 96,195,342 bp
  • C to T, chromosome 4 at 141,813,623 bp
  • T to A, chromosome 4 at 143,654,277 bp
  • A to G, chromosome 4 at 143,811,865 bp
  • C to T, chromosome 4 at 147,674,718 bp
  • G to A, chromosome 5 at 8,977,518 bp
  • T to C, chromosome 5 at 17,672,513 bp
  • T to A, chromosome 5 at 25,203,379 bp
  • T to G, chromosome 5 at 38,300,024 bp
  • A to T, chromosome 5 at 72,552,668 bp
  • T to C, chromosome 5 at 75,162,271 bp
  • A to T, chromosome 5 at 98,004,054 bp
  • G to A, chromosome 5 at 113,273,684 bp
  • C to T, chromosome 5 at 121,292,139 bp
  • G to A, chromosome 5 at 123,579,374 bp
  • G to T, chromosome 6 at 7,678,012 bp
  • A to G, chromosome 6 at 18,406,537 bp
  • A to T, chromosome 6 at 21,973,711 bp
  • A to G, chromosome 6 at 41,357,827 bp
  • T to C, chromosome 6 at 88,617,790 bp
  • A to G, chromosome 6 at 90,378,838 bp
  • G to A, chromosome 6 at 128,323,054 bp
  • A to G, chromosome 6 at 141,249,622 bp
  • G to A, chromosome 6 at 147,257,298 bp
  • A to G, chromosome 7 at 19,396,510 bp
  • G to T, chromosome 7 at 28,546,335 bp
  • T to C, chromosome 7 at 123,265,263 bp
  • A to G, chromosome 7 at 128,750,286 bp
  • T to C, chromosome 7 at 140,323,412 bp
  • A to T, chromosome 7 at 140,504,020 bp
  • G to A, chromosome 9 at 22,578,767 bp
  • G to A, chromosome 9 at 31,327,328 bp
  • A to T, chromosome 9 at 35,235,255 bp
  • A to G, chromosome 9 at 53,236,398 bp
  • A to T, chromosome 9 at 57,036,498 bp
  • T to C, chromosome 9 at 108,570,369 bp
  • C to T, chromosome 9 at 108,861,341 bp
  • G to T, chromosome 9 at 120,016,992 bp
  • T to C, chromosome 10 at 53,548,527 bp
  • G to T, chromosome 10 at 77,894,032 bp
  • T to A, chromosome 11 at 5,016,760 bp
  • T to C, chromosome 11 at 72,445,712 bp
  • C to T, chromosome 11 at 94,845,480 bp
  • T to A, chromosome 11 at 101,434,004 bp
  • T to C, chromosome 11 at 102,285,019 bp
  • T to A, chromosome 11 at 107,635,077 bp
  • T to C, chromosome 11 at 118,019,677 bp
  • T to C, chromosome 12 at 11,274,007 bp
  • T to C, chromosome 12 at 51,595,485 bp
  • C to T, chromosome 12 at 108,202,316 bp
  • T to C, chromosome 13 at 24,982,603 bp
  • T to A, chromosome 13 at 55,604,298 bp
  • T to C, chromosome 13 at 58,323,916 bp
  • A to T, chromosome 14 at 31,154,915 bp
  • A to T, chromosome 14 at 31,667,803 bp
  • G to A, chromosome 15 at 76,065,336 bp
  • T to G, chromosome 15 at 81,792,250 bp
  • T to A, chromosome 16 at 36,887,618 bp
  • G to A, chromosome 16 at 84,812,952 bp
  • T to A, chromosome 16 at 87,923,131 bp
  • G to A, chromosome 16 at 88,562,408 bp
  • A to T, chromosome 17 at 19,811,815 bp
  • A to T, chromosome 17 at 33,777,275 bp
  • A to G, chromosome 17 at 35,200,180 bp
  • T to G, chromosome 17 at 36,030,216 bp
  • G to T, chromosome 17 at 46,477,788 bp
  • A to G, chromosome 18 at 7,211,609 bp
  • A to G, chromosome 18 at 22,516,477 bp
  • C to T, chromosome 18 at 67,401,946 bp
  • T to A, chromosome 19 at 5,748,786 bp
  • T to C, chromosome 19 at 11,871,048 bp
  • C to T, chromosome 19 at 33,620,960 bp
  • A to G, chromosome X at 13,123,508 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4660 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041920-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.