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Strain Name:
C57BL/6J-MtgxR4666Btlr/Mmmh
Stock Number:
041924-MU
Citation ID:
RRID:MMRRC_041924-MU
Other Names:
R4666 (G1), C57BL/6J-MtgxR4666Btlr
Major Collection:

Strain Information

Rptor
Name: regulatory associated protein of MTOR, complex 1
Synonyms: raptor, 4932417H02Rik, Rap
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 74370
Homologene: 80210
Pax6
Name: paired box 6
Synonyms: Pax-6, Dey, Dickie's small eye, 1500038E17Rik, AEY11, Gsfaey11
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 18508
HGNC: HGNC:8620
Homologene: 1212
Vcan
Name: versican
Synonyms: PG-M, hdf, heart defect, 5430420N07Rik, Cspg2, DPEAAE
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 13003
HGNC: HGNC:2464
Homologene: 3228
Chrna4
Name: cholinergic receptor, nicotinic, alpha polypeptide 4
Synonyms: alpha4 nAChR, Acra-4, Acra4, a4 nicotinic receptor, alpha4-nAChR
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 11438
HGNC: HGNC:1958
Homologene: 592
Adipor1
Name: adiponectin receptor 1
Synonyms: 2810031L11Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 72674
Homologene: 69199
Foxa3
Name: forkhead box A3
Synonyms: Tcf-3g, Hnf3g, Hnf-3g, Tcf3g
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 15377
HGNC: HGNC:5023
Homologene: 3308
Galr2
Name: galanin receptor 2
Synonyms: mGalR, GalR2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 14428
HGNC: HGNC:4133
Homologene: 2863
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 58,304,597 bp
  • C to T, chromosome 1 at 74,890,048 bp
  • C to T, chromosome 1 at 106,982,844 bp
  • T to A, chromosome 1 at 134,424,905 bp
  • G to A, chromosome 1 at 172,957,590 bp
  • T to A, chromosome 2 at 27,189,499 bp
  • C to A, chromosome 2 at 30,085,457 bp
  • T to C, chromosome 2 at 44,591,925 bp
  • G to A, chromosome 2 at 71,018,377 bp
  • C to G, chromosome 2 at 71,018,378 bp
  • T to A, chromosome 2 at 87,387,876 bp
  • C to A, chromosome 2 at 105,683,998 bp
  • C to T, chromosome 2 at 122,319,475 bp
  • T to C, chromosome 2 at 129,038,230 bp
  • T to C, chromosome 2 at 142,217,599 bp
  • T to A, chromosome 2 at 167,610,377 bp
  • A to G, chromosome 2 at 181,037,493 bp
  • A to T, chromosome 2 at 181,574,868 bp
  • T to C, chromosome 3 at 19,260,256 bp
  • T to A, chromosome 3 at 116,024,207 bp
  • G to T, chromosome 4 at 43,653,408 bp
  • A to G, chromosome 4 at 84,971,216 bp
  • T to C, chromosome 4 at 88,571,336 bp
  • A to T, chromosome 4 at 107,165,560 bp
  • C to T, chromosome 4 at 116,816,801 bp
  • C to A, chromosome 4 at 127,351,778 bp
  • T to A, chromosome 5 at 24,751,021 bp
  • C to A, chromosome 5 at 30,114,667 bp
  • T to A, chromosome 5 at 30,176,958 bp
  • T to C, chromosome 5 at 30,655,613 bp
  • C to A, chromosome 5 at 76,616,854 bp
  • T to C, chromosome 5 at 86,309,401 bp
  • T to C, chromosome 5 at 108,653,140 bp
  • C to T, chromosome 5 at 110,144,867 bp
  • T to A, chromosome 5 at 115,335,547 bp
  • T to C, chromosome 5 at 124,828,472 bp
  • T to A, chromosome 5 at 134,383,902 bp
  • A to T, chromosome 5 at 143,503,349 bp
  • T to A, chromosome 6 at 12,337,314 bp
  • T to A, chromosome 6 at 12,504,013 bp
  • G to T, chromosome 6 at 50,595,828 bp
  • C to T, chromosome 6 at 53,931,933 bp
  • A to G, chromosome 6 at 104,728,284 bp
  • C to A, chromosome 6 at 142,376,841 bp
  • T to A, chromosome 7 at 5,319,189 bp
  • T to C, chromosome 7 at 5,884,358 bp
  • G to T, chromosome 7 at 19,014,372 bp
  • T to A, chromosome 7 at 23,336,780 bp
  • A to G, chromosome 7 at 30,241,854 bp
  • T to C, chromosome 7 at 34,284,773 bp
  • G to T, chromosome 7 at 64,203,034 bp
  • T to C, chromosome 7 at 85,150,623 bp
  • T to A, chromosome 7 at 114,028,969 bp
  • T to A, chromosome 7 at 127,577,984 bp
  • G to A, chromosome 8 at 70,290,143 bp
  • A to G, chromosome 8 at 70,760,415 bp
  • G to A, chromosome 8 at 70,768,859 bp
  • A to G, chromosome 8 at 79,687,486 bp
  • T to C, chromosome 8 at 85,080,051 bp
  • T to C, chromosome 8 at 99,024,902 bp
  • A to G, chromosome 8 at 116,957,959 bp
  • T to C, chromosome 8 at 123,268,972 bp
  • C to T, chromosome 9 at 21,114,207 bp
  • C to T, chromosome 9 at 35,210,855 bp
  • T to A, chromosome 9 at 39,217,846 bp
  • A to T, chromosome 9 at 42,004,051 bp
  • C to T, chromosome 9 at 96,688,383 bp
  • T to A, chromosome 9 at 99,320,876 bp
  • T to A, chromosome 9 at 105,767,342 bp
  • G to A, chromosome 10 at 18,531,405 bp
  • A to C, chromosome 10 at 20,438,750 bp
  • G to A, chromosome 10 at 59,456,699 bp
  • A to G, chromosome 10 at 60,300,545 bp
  • A to T, chromosome 11 at 6,475,615 bp
  • A to G, chromosome 11 at 44,991,557 bp
  • A to G, chromosome 11 at 45,933,813 bp
  • G to A, chromosome 11 at 67,289,904 bp
  • G to T, chromosome 11 at 67,289,905 bp
  • A to G, chromosome 11 at 75,181,330 bp
  • A to G, chromosome 11 at 96,274,831 bp
  • C to A, chromosome 11 at 97,021,875 bp
  • A to G, chromosome 11 at 116,283,629 bp
  • A to G, chromosome 11 at 116,668,164 bp
  • A to T, chromosome 11 at 119,743,882 bp
  • T to C, chromosome 12 at 69,312,280 bp
  • T to C, chromosome 12 at 101,983,207 bp
  • C to T, chromosome 13 at 62,528,920 bp
  • T to C, chromosome 13 at 67,765,094 bp
  • T to A, chromosome 13 at 73,538,581 bp
  • C to A, chromosome 13 at 89,679,934 bp
  • T to A, chromosome 13 at 93,402,159 bp
  • T to A, chromosome 14 at 53,337,496 bp
  • T to A, chromosome 15 at 11,311,492 bp
  • A to G, chromosome 15 at 35,640,544 bp
  • A to G, chromosome 15 at 73,570,860 bp
  • A to G, chromosome 15 at 80,080,538 bp
  • A to G, chromosome 15 at 86,030,494 bp
  • C to T, chromosome 15 at 89,295,246 bp
  • T to C, chromosome 15 at 103,421,205 bp
  • A to G, chromosome 16 at 23,972,256 bp
  • T to C, chromosome 16 at 36,970,723 bp
  • T to C, chromosome 16 at 58,916,584 bp
  • A to G, chromosome 16 at 59,329,210 bp
  • T to C, chromosome 17 at 15,370,612 bp
  • A to G, chromosome 17 at 24,392,909 bp
  • G to T, chromosome 17 at 27,893,503 bp
  • G to A, chromosome 17 at 36,978,902 bp
  • A to T, chromosome 17 at 37,151,379 bp
  • T to C, chromosome 17 at 80,274,961 bp
  • T to A, chromosome 18 at 30,459,534 bp
  • A to G, chromosome 18 at 78,012,864 bp
  • C to T, chromosome 19 at 17,120,188 bp
  • A to G, chromosome 19 at 37,570,505 bp
  • T to A, chromosome 19 at 46,137,101 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4666 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041924-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.