Strain Name:
C57BL/6J-MtgxR4714Btlr/Mmmh
Stock Number:
041956-MU
Citation ID:
RRID:MMRRC_041956-MU
Other Names:
R4714 (G1), C57BL/6J-MtgxR4714Btlr
Major Collection:

Strain Information

Pax6
Name: paired box 6
Synonyms: Pax-6, Gsfaey11, Dey, AEY11, Dickie's small eye, 1500038E17Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 18508
HGNC: HGNC:8620
Homologene: 1212
Abca12
Name: ATP-binding cassette, sub-family A member 12
Synonyms: 4832428G11Rik, 4833417A11Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 74591
Homologene: 45441
Dlg1
Name: discs large MAGUK scaffold protein 1
Synonyms: SAP97, B130052P05Rik, Dlgh1
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 13383
HGNC: HGNC:2900
Homologene: 20869
Rbm47
Name: RNA binding motif protein 47
Synonyms: 9530077J19Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 245945
Homologene: 36932
Lrp5
Name: low density lipoprotein receptor-related protein 5
Synonyms: LRP7, LR3
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 16973
Homologene: 1746
Psme4
Name: proteasome (prosome, macropain) activator subunit 4
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 103554
Homologene: 113742
Trpm7
Name: transient receptor potential cation channel, subfamily M, member 7
Synonyms: CHAK1, 5033407O22Rik, Ltpr7, 4833414K03Rik, 2310022G15Rik, TRP-PLIK, LTRPC7, CHAK
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 58800
Homologene: 9774
Agrn
Name: agrin
Synonyms: nmf380, Agrin, NMF380
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11603
HGNC: HGNC:329
Homologene: 27907
Msh2
Name: mutS homolog 2
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 17685
HGNC: HGNC:7325
Homologene: 210
Helz
Name: helicase with zinc finger domain
Synonyms: 9430093I07Rik, 3110078M01Rik, 9630002H22Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 78455
Homologene: 8918
Atp2c1
Name: ATPase, Ca++-sequestering
Synonyms: SPCA, D930003G21Rik, 1700121J11Rik, ATP2C1A, PMR1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235574
Homologene: 56672
Ruvbl1
Name: RuvB-like AAA ATPase 1
Synonyms: Tip49a, 2510009G06Rik, Pontin52
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 56505
Homologene: 37839
Ap1g1
Name: adaptor protein complex AP-1, gamma 1 subunit
Synonyms: Adtg, gamma-adaptin, D8Ertd374e
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 11765
HGNC: HGNC:555
Homologene: 47995
Abl1
Name: c-abl oncogene 1, non-receptor tyrosine kinase
Synonyms: c-Abl, E430008G22Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 11350
HGNC: HGNC:76
Homologene: 3783
Tank
Name: TRAF family member-associated Nf-kappa B activator
Synonyms: E430026L09Rik, I-TRAF
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 21353
Homologene: 3081
Zzef1
Name: zinc finger, ZZ-type with EF hand domain 1
Synonyms: 8430405D05Rik, C130099L13Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 195018
Homologene: 9027
Zwint
Name: ZW10 interactor
Synonyms: 2010007E07Rik, 2600001N01Rik, D10Ertd749e
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 52696
Homologene: 48496
Grm7
Name: glutamate receptor, metabotropic 7
Synonyms: mGlu7a receptor, Gpr1g, 6330570A01Rik, mGluR7, E130018M02Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 108073
HGNC: HGNC:4599
Homologene: 20233
Ppfia1
Name: protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 1
Synonyms: Liprin-alpha1, C030014K08Rik, liprin
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233977
HGNC: HGNC:9245
Homologene: 20802
Tenm4
Name: teneurin transmembrane protein 4
Synonyms: Ten-m4, ELM2, Odz4, Doc4, l7Rn3, l(7)-3Rn
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 23966
Homologene: 8034
Adam30
Name: a disintegrin and metallopeptidase domain 30
Synonyms: 4933424D07Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 71078
HGNC: HGNC:208
Homologene: 11038
Dync2h1
Name: dynein cytoplasmic 2 heavy chain 1
Synonyms: b2b414Clo, D330044F14Rik, 4432416O06Rik, DHC1b, D030010H02Rik, Dnchc2, m407Asp, DHC2, m152Asp
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 110350
HGNC: HGNC:2962
Homologene: 14468
Zbtb8os
Name: zinc finger and BTB domain containing 8 opposite strand
Synonyms: 2010001H09Rik, Arch, 2310028N13Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 67106
Homologene: 12083
2700049A03Rik
Name: RIKEN cDNA 2700049A03 gene
Synonyms: talpid3, Ta3
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 76967
Homologene: 8839
Vps13a
Name: vacuolar protein sorting 13A
Synonyms: D330038K10Rik, 4930516E05Rik, 4930543C13Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 271564
VEGA: 19
HGNC: HGNC:1908
Homologene: 22068
Pnkd
Name: paroxysmal nonkinesiogenic dyskinesia
Synonyms: 2810403H05Rik, Brp17, 2210013N15Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 56695
HGNC: HGNC:9153
Homologene: 75045
Enam
Name: enamelin
Synonyms: abte
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 13801
HGNC: HGNC:3344
Homologene: 9698
Sohlh2
Name: spermatogenesis and oogenesis specific basic helix-loop-helix 2
Synonyms: 4933406N12Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 74434
Homologene: 9864
Tnik
Name: TRAF2 and NCK interacting kinase
Synonyms: C530008O15Rik, 1500031A17Rik, 4831440I19Rik, C630040K21Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 665113
Homologene: 77943
Ghr
Name: growth hormone receptor
Synonyms: GHBP, GHR/BP
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 14600
HGNC: HGNC:4263
Homologene: 134
Abcb4
Name: ATP-binding cassette, sub-family B member 4
Synonyms: Mdr2, Pgy2, mdr-2, Pgy-2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 18670
HGNC: HGNC:45
Homologene: 136368
Trpm6
Name: transient receptor potential cation channel, subfamily M, member 6
Synonyms: CHAK2
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 225997
VEGA: 19
Homologene: 9767
Usp40
Name: ubiquitin specific peptidase 40
Synonyms: B230215L03Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 227334
Homologene: 32400
Lrp1b
Name: low density lipoprotein-related protein 1B
Synonyms: 9630004P12Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 94217
HGNC: HGNC:6693
Homologene: 56810
Atp11b
Name: ATPase, class VI, type 11B
Synonyms: 1110019I14Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 76295
Homologene: 32919
Cgn
Name: cingulin
Synonyms: 6330408J11Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 70737
Homologene: 41394
Fmo2
Name: flavin containing monooxygenase 2
Synonyms: 2310042I22Rik, 2310008D08Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 55990
HGNC: HGNC:3770
Homologene: 86882
Sorbs2
Name: sorbin and SH3 domain containing 2
Synonyms: 9430041O17Rik, 2010203O03Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 234214
Homologene: 83295
Pnpla8
Name: patatin-like phospholipase domain containing 8
Synonyms: iPLA2 gamma, 1200006O19Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 67452
Homologene: 12136
Metrnl
Name: meteorin, glial cell differentiation regulator-like
Synonyms: 9430048M07Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 210029
Homologene: 16993
Frmpd1
Name: FERM and PDZ domain containing 1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 666060
Homologene: 8939
4930486L24Rik
Name: RIKEN cDNA 4930486L24 gene
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 214639
VEGA: 13
Homologene: 138410
3930402G23Rik
Name: RIKEN cDNA 3930402G23 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 665306
Vmn2r89
Name: vomeronasal 2, receptor 89
Synonyms: V2r10, V2r11
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 22301
Kcnc2
Name: potassium voltage gated channel, Shaw-related subfamily, member 2
Synonyms: KShIIIA, Kv3.2
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 268345
VEGA: 10
HGNC: HGNC:6234
Homologene: 71199
Ttc28
Name: tetratricopeptide repeat domain 28
Synonyms: 2310015L07Rik, TPRBK
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 209683
Homologene: 41023
Oas2
Name: 2'-5' oligoadenylate synthetase 2
Synonyms: Oasl11, 2'-5' oligoadenylate synthetase-like 11
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 246728
HGNC: HGNC:8087
Homologene: 49478
Zfp467
Name: zinc finger protein 467
Synonyms: EZI, MNCb-3350, 1190001I08Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 68910
Homologene: 10758
Rasip1
Name: Ras interacting protein 1
Synonyms: Rain, 2610025P08Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 69903
Homologene: 9847
Hif3a
Name: hypoxia inducible factor 3, alpha subunit
Synonyms: MOP7, Nepas, bHLHe17
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 53417
Homologene: 9646
Alkbh6
Name: alkB homolog 6
Synonyms: Abh6
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233065
Homologene: 15555
Or2n1d
Name: olfactory receptor family 2 subfamily N member 1D
Synonyms: Olfr136, GA_x6K02T2PSCP-2779375-2780313, MOR256-7
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 258803
Homologene: 119758
Gmppb
Name: GDP-mannose pyrophosphorylase B
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 331026
Homologene: 5578
Flt4
Name: FMS-like tyrosine kinase 4
Synonyms: VEGFR-3, Flt-4, VEGFR3
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 14257
HGNC: HGNC:3767
Homologene: 7321
Vps52
Name: VPS52 GARP complex subunit
Synonyms: tcl-w5, tclw5, ARE1, D430041K17Rik, Sacm2l
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 224705
Homologene: 5756
Necab2
Name: N-terminal EF-hand calcium binding protein 2
Synonyms: Necab2, Efcbp2
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 117148
Homologene: 62200
Or1j1
Name: olfactory receptor family 1 subfamily J member 1
Synonyms: GA_x6K02T2NLDC-33507606-33506665, MOR136-14, Y71, Olfr3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 18328
HGNC: HGNC:8208
Homologene: 66579
Dcun1d1
Name: defective in cullin neddylation 1 domain containing 1
Synonyms: Tes3, DCN1, defective in cullin neddylation 1, domain containing 1 (S. cerevisiae), Rp42, pTes3, SCCRO
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 114893
Homologene: 10773
Vmn1r202
Name: vomeronasal 1 receptor 202
Synonyms: V1ri7
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 171258
Homologene: 110880
Or4k6
Name: olfactory receptor family 4 subfamily K member 6
Synonyms: MOR246-5, GA_x6K02T2PMLR-5936117-5935137, Olfr731
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 258360
Homologene: 84579
Ifit1
Name: interferon-induced protein with tetratricopeptide repeats 1
Synonyms: Ifi56, ISG56
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 15957
Homologene: 22462
Champ1
Name: chromosome alignment maintaining phosphoprotein 1
Synonyms: D8Ertd569e, Zfp828, D8Ertd457e
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 101994
Homologene: 18780
Tymp
Name: thymidine phosphorylase
Synonyms: 2900072D10Rik, gliostatin, Ecgf1, Pdgfec, PD-ECGF, PDECGF
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 72962
VEGA: 15
HGNC: HGNC:3148
Homologene: 1474
Cpm
Name: carboxypeptidase M
Synonyms: 1110060I01Rik, 5730456K23Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 70574
HGNC: HGNC:2311
Homologene: 35367
Defb8
Name: defensin beta 8
Synonyms: Defr1, mBD8
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 244334
A430093F15Rik
Name: RIKEN cDNA A430093F15 gene
Synonyms: LOC269062
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 403202
Or4k1
Name: olfactory receptor family 4 subfamily K member 1
Synonyms: GA_x6K02T2PMLR-5831021-5830086, MOR246-1P, Olfr728
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 258039
Homologene: 74224
Or6c216
Name: olfactory receptor family 6 subfamily C member 216
Synonyms: MOR110-1, GA_x6K02T2PULF-11521598-11520666, Olfr812
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 258791
Homologene: 73931
Haus4
Name: HAUS augmin-like complex, subunit 4
Synonyms: D14Ertd500e, 9430093H08Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 219072
VEGA: 14
Homologene: 9854
Dcaf15
Name: DDB1 and CUL4 associated factor 15
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 212123
Homologene: 18182
Gm1988
Name: predicted gene 1988
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 100038967
Homologene: 80005
RP24-390L20.1
Name:
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Zfp36l1
Name: zinc finger protein 36, C3H type-like 1
Synonyms: cMG1, D530020L18Rik, TIS11b, Brf1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 12192
VEGA: 12
HGNC: HGNC:1107
Homologene: 31276
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 32,520,682 bp
  • A to G, chromosome 1 at 71,321,450 bp
  • C to T, chromosome 1 at 74,351,782 bp
  • C to T, chromosome 1 at 87,967,179 bp
  • T to A, chromosome 1 at 162,876,716 bp
  • C to T, chromosome 2 at 31,797,731 bp
  • A to T, chromosome 2 at 36,813,035 bp
  • A to T, chromosome 2 at 41,110,759 bp
  • C to T, chromosome 2 at 61,650,229 bp
  • A to G, chromosome 2 at 105,695,400 bp
  • G to A, chromosome 2 at 126,840,783 bp
  • A to G, chromosome 3 at 28,594,077 bp
  • G to A, chromosome 3 at 35,834,394 bp
  • C to T, chromosome 3 at 35,895,670 bp
  • A to G, chromosome 3 at 55,190,529 bp
  • C to A, chromosome 3 at 94,779,438 bp
  • A to T, chromosome 3 at 98,162,854 bp
  • A to G, chromosome 4 at 45,284,785 bp
  • T to C, chromosome 4 at 129,341,764 bp
  • A to G, chromosome 4 at 156,178,472 bp
  • A to G, chromosome 5 at 8,930,906 bp
  • A to T, chromosome 5 at 66,025,052 bp
  • A to G, chromosome 5 at 88,503,536 bp
  • G to A, chromosome 5 at 111,285,229 bp
  • A to T, chromosome 5 at 120,733,472 bp
  • A to G, chromosome 6 at 48,427,817 bp
  • T to A, chromosome 6 at 88,484,430 bp
  • G to A, chromosome 6 at 111,080,422 bp
  • A to G, chromosome 7 at 17,056,271 bp
  • T to C, chromosome 7 at 30,314,225 bp
  • A to G, chromosome 7 at 39,173,728 bp
  • CGG to CGGG, chromosome 7 at 45,632,396 bp
  • A to G, chromosome 7 at 96,894,924 bp
  • A to G, chromosome 7 at 144,477,975 bp
  • C to T, chromosome 8 at 10,926,393 bp
  • T to C, chromosome 8 at 13,878,063 bp
  • A to G, chromosome 8 at 19,447,559 bp
  • A to G, chromosome 8 at 45,795,293 bp
  • T to C, chromosome 8 at 84,102,216 bp
  • T to A, chromosome 8 at 109,829,620 bp
  • T to C, chromosome 8 at 119,467,595 bp
  • G to T, chromosome 9 at 7,118,932 bp
  • A to T, chromosome 9 at 105,439,440 bp
  • A to G, chromosome 9 at 108,052,439 bp
  • T to A, chromosome 10 at 72,656,400 bp
  • A to G, chromosome 10 at 92,815,918 bp
  • T to C, chromosome 10 at 112,455,828 bp
  • T to C, chromosome 10 at 117,675,985 bp
  • A to T, chromosome 10 at 129,842,945 bp
  • T to A, chromosome 11 at 30,832,573 bp
  • T to A, chromosome 11 at 49,627,207 bp
  • T to A, chromosome 11 at 72,837,212 bp
  • T to C, chromosome 11 at 107,626,716 bp
  • C to T, chromosome 11 at 121,716,013 bp
  • T to C, chromosome 12 at 44,295,913 bp
  • G to T, chromosome 12 at 71,164,546 bp
  • A to T, chromosome 12 at 71,164,547 bp
  • T to C, chromosome 12 at 80,110,496 bp
  • T to A, chromosome 13 at 22,501,807 bp
  • A to G, chromosome 13 at 60,844,318 bp
  • C to T, chromosome 13 at 74,798,715 bp
  • A to G, chromosome 14 at 50,139,979 bp
  • C to A, chromosome 14 at 50,238,367 bp
  • T to C, chromosome 14 at 51,452,231 bp
  • T to C, chromosome 14 at 54,542,120 bp
  • T to A, chromosome 15 at 3,320,397 bp
  • A to G, chromosome 15 at 89,376,307 bp
  • A to T, chromosome 16 at 31,790,261 bp
  • A to G, chromosome 17 at 33,961,179 bp
  • A to C, chromosome 17 at 38,335,840 bp
  • A to G, chromosome 17 at 87,718,789 bp
  • T to C, chromosome 19 at 3,659,454 bp
  • T to G, chromosome 19 at 10,785,379 bp
  • A to T, chromosome 19 at 16,749,856 bp
  • C to A, chromosome 19 at 18,854,200 bp
  • A to G, chromosome 19 at 34,648,163 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4714 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041956-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.