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Strain Name:
C57BL/6J-MtgxR4738Btlr/Mmmh
Stock Number:
041964-MU
Citation ID:
RRID:MMRRC_041964-MU
Other Names:
R4738 (G1), C57BL/6J-MtgxR4738Btlr
Major Collection:

Strain Information

Tyr
Name: tyrosinase
Synonyms: skc35, Oca1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 22173
Homologene: 30969
Met
Name: met proto-oncogene
Synonyms: HGF receptor, c-Met, Par4
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 17295
HGNC: HGNC:7029
Homologene: 206
Angpt2
Name: angiopoietin 2
Synonyms: Ang2, Ang-2
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 11601
HGNC: HGNC:485
Homologene: 22401
Areg
Name: amphiregulin
Synonyms: AR, Sdgf, Mcub
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 11839
HGNC: HGNC:651
Homologene: 1252
Lhx9
Name: LIM homeobox protein 9
Synonyms: LH2B, Lhx9 alpha, 3110009O07Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 16876
Homologene: 7816
Abtb3
Name: ankyrin repeat and BTB domain containing 3
Synonyms: 6330404E16Rik, Btbd11
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 74007
Homologene: 72536
Tlk2
Name: tousled-like kinase 2 (Arabidopsis)
Synonyms: protein kinase U-alpha, PKUalpha, 4933403M19Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 24086
Homologene: 4993
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 39,402,878 bp
  • A to T, chromosome 1 at 97,923,132 bp
  • A to G, chromosome 1 at 127,934,436 bp
  • A to G, chromosome 1 at 138,832,748 bp
  • T to C, chromosome 1 at 158,957,012 bp
  • G to A, chromosome 1 at 170,311,886 bp
  • T to C, chromosome 1 at 177,872,020 bp
  • T to C, chromosome 2 at 24,277,491 bp
  • T to C, chromosome 2 at 24,963,131 bp
  • A to G, chromosome 2 at 28,545,416 bp
  • A to C, chromosome 2 at 52,187,482 bp
  • T to C, chromosome 2 at 76,880,546 bp
  • A to G, chromosome 2 at 80,424,597 bp
  • T to A, chromosome 2 at 83,915,395 bp
  • A to G, chromosome 2 at 85,070,129 bp
  • G to A, chromosome 2 at 90,440,643 bp
  • A to G, chromosome 2 at 118,790,326 bp
  • T to A, chromosome 2 at 120,452,594 bp
  • A to G, chromosome 2 at 168,668,181 bp
  • C to T, chromosome 3 at 32,708,922 bp
  • T to C, chromosome 3 at 93,797,089 bp
  • T to C, chromosome 3 at 105,979,918 bp
  • T to C, chromosome 3 at 116,539,132 bp
  • A to G, chromosome 4 at 86,600,749 bp
  • G to T, chromosome 4 at 139,362,202 bp
  • T to C, chromosome 4 at 156,199,862 bp
  • A to G, chromosome 5 at 22,010,215 bp
  • T to A, chromosome 5 at 31,901,142 bp
  • A to G, chromosome 5 at 32,975,322 bp
  • T to C, chromosome 5 at 91,146,724 bp
  • A to G, chromosome 5 at 104,973,983 bp
  • T to C, chromosome 5 at 129,769,386 bp
  • T to A, chromosome 5 at 140,430,078 bp
  • T to C, chromosome 6 at 17,491,541 bp
  • T to C, chromosome 6 at 35,051,376 bp
  • T to A, chromosome 6 at 40,477,980 bp
  • C to A, chromosome 6 at 87,054,747 bp
  • G to T, chromosome 7 at 30,563,348 bp
  • G to A, chromosome 7 at 44,541,329 bp
  • T to C, chromosome 7 at 87,492,647 bp
  • A to G, chromosome 7 at 101,297,178 bp
  • T to A, chromosome 7 at 102,710,171 bp
  • C to T, chromosome 7 at 104,149,718 bp
  • C to T, chromosome 7 at 105,758,673 bp
  • A to T, chromosome 7 at 108,211,994 bp
  • A to G, chromosome 7 at 119,787,732 bp
  • G to T, chromosome 8 at 10,373,527 bp
  • C to T, chromosome 8 at 18,741,059 bp
  • T to A, chromosome 8 at 40,464,142 bp
  • T to A, chromosome 8 at 69,103,410 bp
  • C to T, chromosome 8 at 69,864,969 bp
  • T to C, chromosome 8 at 76,909,307 bp
  • C to T, chromosome 8 at 79,992,570 bp
  • T to C, chromosome 9 at 21,774,619 bp
  • T to C, chromosome 9 at 79,699,282 bp
  • A to G, chromosome 9 at 115,216,574 bp
  • A to G, chromosome 10 at 39,874,149 bp
  • C to T, chromosome 10 at 85,627,248 bp
  • T to A, chromosome 10 at 130,447,070 bp
  • C to T, chromosome 11 at 45,954,191 bp
  • T to C, chromosome 11 at 59,372,945 bp
  • A to T, chromosome 11 at 69,601,204 bp
  • G to T, chromosome 11 at 73,375,350 bp
  • T to A, chromosome 11 at 94,239,076 bp
  • G to A, chromosome 11 at 98,378,143 bp
  • T to A, chromosome 11 at 105,256,882 bp
  • T to C, chromosome 11 at 120,783,964 bp
  • A to T, chromosome 12 at 69,969,381 bp
  • T to C, chromosome 12 at 76,576,914 bp
  • G to T, chromosome 12 at 85,140,387 bp
  • T to A, chromosome 13 at 54,795,064 bp
  • A to T, chromosome 13 at 67,317,165 bp
  • T to G, chromosome 13 at 74,357,068 bp
  • T to A, chromosome 14 at 56,026,999 bp
  • C to T, chromosome 14 at 104,510,383 bp
  • A to T, chromosome 15 at 63,826,801 bp
  • C to T, chromosome 15 at 77,488,641 bp
  • G to A, chromosome 15 at 78,605,874 bp
  • C to T, chromosome 16 at 18,251,286 bp
  • T to C, chromosome 16 at 59,036,195 bp
  • A to G, chromosome 17 at 33,215,810 bp
  • T to C, chromosome 18 at 13,844,054 bp
  • T to C, chromosome 18 at 37,403,415 bp
  • A to G, chromosome 18 at 50,090,502 bp
  • T to C, chromosome 18 at 64,545,180 bp
  • A to T, chromosome 18 at 67,884,830 bp
  • A to G, chromosome 19 at 7,604,099 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4738 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041964-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.