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Strain Name:
C57BL/6J-MtgxR4722Btlr/Mmmh
Stock Number:
041987-MU
Citation ID:
RRID:MMRRC_041987-MU
Other Names:
R4722 (G1), C57BL/6J-MtgxR4722Btlr
Major Collection:

Strain Information

Chd7
Name: chromodomain helicase DNA binding protein 7
Synonyms: GENA 60, GENA 47, Gena 52, Cyn, A730019I05Rik, WBE1, Whi, Todo, Obt, Mt, Lda, Flo, Edy, Dz, Cycn
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 320790
Homologene: 19067
Zic4
Name: zinc finger protein of the cerebellum 4
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 22774
Homologene: 32075
Oxr1
Name: oxidation resistance 1
Synonyms: C7B, C7, 2210416C20Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 170719
VEGA: 15
Homologene: 24993
Rapgef2
Name: Rap guanine nucleotide exchange factor (GEF) 2
Synonyms: 5830453M24Rik, Pdzgef1, RA-GEF-1, CNRasGEF, nRapGEP
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 76089
Homologene: 35477
Enpp2
Name: ectonucleotide pyrophosphatase/phosphodiesterase 2
Synonyms: Autotaxin, Npps2, PD-Ialpha, Pdnp2, ATX
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 18606
HGNC: HGNC:3357
Homologene: 4526
Tnrc6a
Name: trinucleotide repeat containing 6a
Synonyms: CAGH26, 3110054G10Rik, D130023A07Rik, 2010321I05Rik, Tnrc6
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233833
Homologene: 41399
Rnmt
Name: RNA (guanine-7-) methyltransferase
Synonyms: 2610002P10Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 67897
Homologene: 2816
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 43,774,846 bp
  • T to C, chromosome 1 at 72,611,337 bp
  • A to T, chromosome 1 at 156,302,375 bp
  • G to T, chromosome 1 at 191,556,841 bp
  • T to A, chromosome 2 at 29,524,435 bp
  • T to A, chromosome 2 at 66,700,884 bp
  • T to G, chromosome 2 at 88,756,012 bp
  • T to C, chromosome 2 at 88,929,980 bp
  • C to T, chromosome 2 at 119,613,011 bp
  • C to T, chromosome 2 at 131,193,351 bp
  • T to G, chromosome 2 at 177,104,746 bp
  • A to G, chromosome 3 at 79,069,173 bp
  • A to T, chromosome 3 at 153,411,529 bp
  • T to A, chromosome 4 at 8,822,445 bp
  • T to C, chromosome 4 at 25,799,734 bp
  • T to G, chromosome 4 at 45,803,472 bp
  • T to A, chromosome 4 at 113,893,855 bp
  • A to T, chromosome 4 at 116,805,200 bp
  • T to A, chromosome 4 at 116,816,872 bp
  • T to C, chromosome 4 at 118,808,949 bp
  • T to A, chromosome 5 at 3,543,949 bp
  • A to G, chromosome 5 at 33,146,711 bp
  • T to C, chromosome 5 at 64,263,557 bp
  • G to A, chromosome 5 at 69,341,201 bp
  • A to T, chromosome 5 at 123,447,202 bp
  • A to G, chromosome 5 at 143,321,781 bp
  • C to T, chromosome 6 at 87,383,525 bp
  • T to C, chromosome 6 at 121,038,525 bp
  • C to T, chromosome 7 at 19,812,523 bp
  • G to A, chromosome 7 at 30,583,202 bp
  • T to A, chromosome 7 at 44,313,214 bp
  • T to A, chromosome 7 at 107,097,570 bp
  • T to A, chromosome 7 at 108,159,238 bp
  • T to C, chromosome 7 at 123,192,090 bp
  • A to G, chromosome 7 at 140,099,567 bp
  • A to T, chromosome 8 at 84,258,810 bp
  • T to A, chromosome 8 at 123,525,635 bp
  • C to T, chromosome 9 at 83,868,593 bp
  • G to T, chromosome 9 at 91,379,204 bp
  • TGTCGCCG to TG, chromosome 9 at 111,272,084 bp
  • T to G, chromosome 10 at 81,341,295 bp
  • T to A, chromosome 10 at 122,449,001 bp
  • T to A, chromosome 11 at 34,695,471 bp
  • A to T, chromosome 11 at 81,968,183 bp
  • T to C, chromosome 11 at 83,283,418 bp
  • C to T, chromosome 11 at 109,434,631 bp
  • G to T, chromosome 12 at 55,061,137 bp
  • G to T, chromosome 13 at 3,928,185 bp
  • T to C, chromosome 13 at 22,883,908 bp
  • A to G, chromosome 13 at 27,660,875 bp
  • T to C, chromosome 13 at 41,767,347 bp
  • A to G, chromosome 13 at 67,042,113 bp
  • G to T, chromosome 13 at 100,307,072 bp
  • A to G, chromosome 14 at 55,854,395 bp
  • T to C, chromosome 15 at 41,813,649 bp
  • T to G, chromosome 15 at 54,887,589 bp
  • C to A, chromosome 15 at 91,688,901 bp
  • A to T, chromosome 15 at 101,528,411 bp
  • A to G, chromosome 17 at 24,265,429 bp
  • T to C, chromosome 17 at 35,958,041 bp
  • C to A, chromosome 18 at 25,174,715 bp
  • C to T, chromosome 18 at 37,091,880 bp
  • G to A, chromosome 18 at 60,564,391 bp
  • T to A, chromosome 18 at 68,305,881 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4722 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041987-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.