Strain Name:
C57BL/6J-MtgxR4799Btlr/Mmmh
Stock Number:
041997-MU
Citation ID:
RRID:MMRRC_041997-MU
Other Names:
R4799 (G1), C57BL/6J-MtgxR4799Btlr
Major Collection:

Gene Information

Gene Symbol: Utrn [MGI:104631] (Mus musculus (mouse))
Name: utrophin
Synonyms: DRP, G-utrophin, Dmdl
Chromosome: 10
Alteration at locus: Chemically Induced
Gene Symbol: Mtmr3 [MGI:1921552] (Mus musculus (mouse))
Name: myotubularin related protein 3
Synonyms: 1700092A20Rik, FYVE-DSP1, ZFYVE10
Chromosome: 11
Alteration at locus: Chemically Induced
Gene Symbol: Csf2 [MGI:1339752] (Mus musculus (mouse))
Name: colony stimulating factor 2 (granulocyte-macrophage)
Synonyms: MGI-IGM, GMCSF, Csfgm, Gm-CSf
Chromosome: 11
Alteration at locus: Chemically Induced
Gene Symbol: Ddc [MGI:94876] (Mus musculus (mouse))
Name: dopa decarboxylase
Synonyms: Aadc, aromatic L-amino acid decarboxylase
Chromosome: 11
Alteration at locus: Chemically Induced
Gene Symbol: Plin2 [MGI:87920] (Mus musculus (mouse))
Name: perilipin 2
Synonyms: Adrp, adipophilin, ADPH, Adfp
Chromosome: 4
Alteration at locus: Chemically Induced
Gene Symbol: Slco1a5 [MGI:1351865] (Mus musculus (mouse))
Name: solute carrier organic anion transporter family, member 1a5
Synonyms: Slc21a7, Oatp3
Chromosome: 6
Alteration at locus: Chemically Induced
Gene Symbol: Gcm2 [MGI:1861438] (Mus musculus (mouse))
Name: glial cells missing homolog 2
Synonyms: Gcm1-rs2
Chromosome: 13
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 24,744,975 bp
  • C to A, chromosome 1 at 63,160,949 bp
  • T to C, chromosome 1 at 67,142,986 bp
  • C to A, chromosome 1 at 75,489,501 bp
  • TAAAAAAAAA to TAAAAAAA, chromosome 1 at 88,215,928 bp
  • G to A, chromosome 1 at 100,358,725 bp
  • T to A, chromosome 1 at 143,627,875 bp
  • A to G, chromosome 1 at 190,153,472 bp
  • C to T, chromosome 2 at 11,755,747 bp
  • T to A, chromosome 2 at 13,287,024 bp
  • A to G, chromosome 2 at 13,351,058 bp
  • A to T, chromosome 2 at 59,342,105 bp
  • T to A, chromosome 2 at 75,977,385 bp
  • T to C, chromosome 2 at 84,693,431 bp
  • C to T, chromosome 2 at 86,732,674 bp
  • C to T, chromosome 2 at 89,721,362 bp
  • T to C, chromosome 2 at 140,660,166 bp
  • A to G, chromosome 2 at 156,134,449 bp
  • C to T, chromosome 3 at 14,536,096 bp
  • G to A, chromosome 3 at 28,717,532 bp
  • C to T, chromosome 3 at 84,903,861 bp
  • T to A, chromosome 3 at 98,852,865 bp
  • C to T, chromosome 3 at 105,658,766 bp
  • C to T, chromosome 3 at 159,897,445 bp
  • A to T, chromosome 4 at 24,502,944 bp
  • A to T, chromosome 4 at 24,580,052 bp
  • G to C, chromosome 4 at 49,649,962 bp
  • A to G, chromosome 4 at 76,091,532 bp
  • G to A, chromosome 4 at 86,668,491 bp
  • G to A, chromosome 4 at 109,366,530 bp
  • A to G, chromosome 4 at 116,972,972 bp
  • C to T, chromosome 4 at 118,954,488 bp
  • A to T, chromosome 4 at 119,621,660 bp
  • A to T, chromosome 4 at 126,347,872 bp
  • A to G, chromosome 4 at 126,839,669 bp
  • A to G, chromosome 4 at 139,192,999 bp
  • A to G, chromosome 4 at 140,977,466 bp
  • G to T, chromosome 4 at 155,659,772 bp
  • T to C, chromosome 5 at 3,557,815 bp
  • T to A, chromosome 5 at 4,083,256 bp
  • C to A, chromosome 5 at 21,250,943 bp
  • G to T, chromosome 5 at 31,167,994 bp
  • A to G, chromosome 5 at 38,224,779 bp
  • A to G, chromosome 5 at 87,590,168 bp
  • A to T, chromosome 5 at 107,620,247 bp
  • T to A, chromosome 5 at 115,113,090 bp
  • C to T, chromosome 5 at 123,302,772 bp
  • A to T, chromosome 5 at 125,037,060 bp
  • G to A, chromosome 5 at 136,990,800 bp
  • G to T, chromosome 6 at 49,045,483 bp
  • C to T, chromosome 6 at 115,845,322 bp
  • T to C, chromosome 6 at 142,248,807 bp
  • T to A, chromosome 7 at 19,065,858 bp
  • T to C, chromosome 7 at 23,632,944 bp
  • A to T, chromosome 7 at 73,484,436 bp
  • A to T, chromosome 7 at 103,787,481 bp
  • T to A, chromosome 7 at 141,847,716 bp
  • A to T, chromosome 7 at 143,534,463 bp
  • A to T, chromosome 8 at 8,623,104 bp
  • A to G, chromosome 8 at 72,058,094 bp
  • A to C, chromosome 8 at 72,725,643 bp
  • A to G, chromosome 8 at 83,935,573 bp
  • G to T, chromosome 8 at 107,076,675 bp
  • A to G, chromosome 8 at 123,288,202 bp
  • A to G, chromosome 9 at 70,589,057 bp
  • T to C, chromosome 9 at 107,998,699 bp
  • A to G, chromosome 9 at 110,863,796 bp
  • A to G, chromosome 9 at 121,255,150 bp
  • T to C, chromosome 10 at 12,750,069 bp
  • T to A, chromosome 10 at 22,181,300 bp
  • C to T, chromosome 10 at 57,808,015 bp
  • A to C, chromosome 10 at 62,665,737 bp
  • T to G, chromosome 10 at 78,956,097 bp
  • C to T, chromosome 10 at 116,048,218 bp
  • C to A, chromosome 10 at 118,205,153 bp
  • G to A, chromosome 11 at 4,487,764 bp
  • T to C, chromosome 11 at 11,846,632 bp
  • A to T, chromosome 11 at 46,677,267 bp
  • T to C, chromosome 11 at 54,249,480 bp
  • G to T, chromosome 11 at 72,859,623 bp
  • A to T, chromosome 11 at 94,048,516 bp
  • T to A, chromosome 11 at 94,048,517 bp
  • T to C, chromosome 11 at 118,183,942 bp
  • C to T, chromosome 12 at 38,114,568 bp
  • C to G, chromosome 12 at 70,169,064 bp
  • A to G, chromosome 12 at 75,899,167 bp
  • A to T, chromosome 12 at 100,850,343 bp
  • T to A, chromosome 13 at 23,011,846 bp
  • T to C, chromosome 13 at 24,878,040 bp
  • T to C, chromosome 13 at 40,001,892 bp
  • T to C, chromosome 13 at 41,103,494 bp
  • G to A, chromosome 13 at 95,513,969 bp
  • C to T, chromosome 14 at 14,120,126 bp
  • C to A, chromosome 14 at 31,377,495 bp
  • A to G, chromosome 14 at 49,081,070 bp
  • T to C, chromosome 14 at 70,139,554 bp
  • G to A, chromosome 14 at 75,339,423 bp
  • T to C, chromosome 15 at 54,910,094 bp
  • T to C, chromosome 15 at 77,091,818 bp
  • T to C, chromosome 15 at 85,376,708 bp
  • A to G, chromosome 16 at 38,591,162 bp
  • T to A, chromosome 16 at 57,192,628 bp
  • C to T, chromosome 17 at 5,339,221 bp
  • T to C, chromosome 17 at 13,120,895 bp
  • T to A, chromosome 17 at 17,088,436 bp
  • T to A, chromosome 17 at 20,462,629 bp
  • T to A, chromosome 17 at 21,434,140 bp
  • A to T, chromosome 17 at 21,818,985 bp
  • T to A, chromosome 17 at 31,632,852 bp
  • T to C, chromosome 17 at 33,838,213 bp
  • G to A, chromosome 17 at 35,150,042 bp
  • T to C, chromosome 17 at 46,500,844 bp
  • T to C, chromosome 17 at 46,677,300 bp
  • A to G, chromosome 18 at 37,355,653 bp
  • A to G, chromosome 18 at 42,395,987 bp
  • C to A, chromosome 18 at 87,756,449 bp
  • A to G, chromosome 19 at 8,344,404 bp
  • A to T, chromosome 19 at 39,773,849 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4799 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041997-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.