Strain Name:
C57BL/6J-MtgxR4558Btlr/Mmmh
Stock Number:
042005-MU
Citation ID:
RRID:MMRRC_042005-MU
Other Names:
R4558 (G1), C57BL/6J-MtgxR4558Btlr
Major Collection:

Gene Information

Sgsm1
Name: small G protein signaling modulator 1
Synonyms: D5Bwg1524e, 2410098H20Rik, Rutbc2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 52850
Homologene: 64485
Tmem161b
Name: transmembrane protein 161B
Synonyms: 2810446P07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 72745
Homologene: 14519
Fam122b
Name: family with sequence similarity 122, member B
Synonyms: 4632404H22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
Alteration at locus: Chemically Induced
NCBI: 78755
Homologene: 12700
Bmpr2
Name: bone morphogenetic protein receptor, type II (serine/threonine kinase)
Synonyms: BMPR-II, BMP-2, 2610024H22Rik, BMPRII
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 12168
HGNC: HGNC:1078
Homologene: 929
Casp12
Name: caspase 12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 12364
Homologene: 7654
Med13
Name: mediator complex subunit 13
Synonyms: Trap240, 1110067M05Rik, Thrap1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 327987
Homologene: 21067
Upf2
Name: UPF2 regulator of nonsense transcripts homolog (yeast)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 326622
Homologene: 6101
Cacna2d3
Name: calcium channel, voltage-dependent, alpha2/delta subunit 3
Synonyms: alpha 2 delta-3, alpha2delta3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 12294
Homologene: 74929
Rasa3
Name: RAS p21 protein activator 3
Synonyms: R-Ras gap, GAPIII activator 3, hlb381, GAPIII, Ras GTPase-activating protein III, scat
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 19414
Homologene: 7217
Eml2
Name: echinoderm microtubule associated protein like 2
Synonyms: 1600029N02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 72205
Homologene: 8125
Ncapg
Name: non-SMC condensin I complex, subunit G
Synonyms: 5730507H05Rik, MFT.M05.13, Hcapg
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 54392
Homologene: 44071
Nbeal1
Name: neurobeachin like 1
Synonyms: ALS2CR17, 2310076G13Rik, A530083I02Rik, A530050O19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 269198
Homologene: 16453
Atad2b
Name: ATPase family, AAA domain containing 2B
Synonyms: D530031C13Rik, 1110014E10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 320817
VEGA: 12
Homologene: 86351
Kalrn
Name: kalirin, RhoGEF kinase
Synonyms: 2210407G14Rik, Hapip, E530005C20Rik, LOC224126
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 545156
HGNC: HGNC:4814
Homologene: 57160
Cnbd1
Name: cyclic nucleotide binding domain containing 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 435772
Homologene: 27843
Pcdhb4
Name: protocadherin beta 4
Synonyms: Pcdhb5A, PcdhbD
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 93875
Homologene: 62176
Pramef12
Name: PRAME family member 12
Synonyms: 4930569K13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 77632
Homologene: 135701
Fsip2
Name: fibrous sheath-interacting protein 2
Synonyms: OTTMUSG00000013335
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 241516
Homologene: 110349
Obscn
Name: obscurin, cytoskeletal calmodulin and titin-interacting RhoGEF
Synonyms: LOC380698, OTTMUSG00000005786
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 380698
Homologene: 70869
Fam227b
Name: family with sequence similarity 227, member B
Synonyms: 4930525F21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 75823
Homologene: 27384
Serpinb9c
Name: serine (or cysteine) peptidase inhibitor, clade B, member 9c
Synonyms: NK9, Spi11, 3830421J05Rik, ovalbumin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 20707
HGNC: HGNC:8955
Ppp4r4
Name: protein phosphatase 4, regulatory subunit 4
Synonyms: 8430415E04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 74521
Homologene: 12571
Gm10764
Name: predicted gene 10764
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
Psd4
Name: pleckstrin and Sec7 domain containing 4
Synonyms: EFA6B, SEC7 homolog
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 215632
Homologene: 8261
Alox12
Name: arachidonate 12-lipoxygenase
Synonyms: P-12LO, 9930022G08Rik, Alox12p
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 11684
HGNC: HGNC:429
Homologene: 560
Acot11
Name: acyl-CoA thioesterase 11
Synonyms: Them1, BFIT1, 2010309H15Rik, Thea, 1110020M10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 329910
Homologene: 11977
Fam189a2
Name: family with sequence similarity 189, member A2
Synonyms: LOC381217
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 381217
VEGA: 19
Homologene: 3540
A830018L16Rik
Name: RIKEN cDNA A830018L16 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 320492
Homologene: 14194
H2-Q6
Name: histocompatibility 2, Q region locus 6
Synonyms: H-2Q6, Qa-6, 0610037M15Rik, Qa6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 110557
HGNC: HGNC:4931
Homologene: 128352
Kng1
Name: kininogen 1
Synonyms: H-kininigen, L-kininogen
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 16644
HGNC: HGNC:6383
Homologene: 88343
Hecw1
Name: HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1
Synonyms: E130207I19Rik, NEDL1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 94253
VEGA: 13
Homologene: 9004
Catsperb
Name: cation channel sperm associated auxiliary subunit beta
Synonyms: 4932415G16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 271036
VEGA: 12
Homologene: 81904
1700017G19Rik
Name: RIKEN cDNA 1700017G19 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 100040882
Vmn1r207-ps
Name: vomeronasal 1 receptor 207, pseudogene
Synonyms: Gm11312
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 432735
Homologene: 110880
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to A, chromosome 1 at 11,972,076 bp
  • T to A, chromosome 1 at 59,845,692 bp
  • C to T, chromosome 1 at 60,281,310 bp
  • A to G, chromosome 2 at 5,973,593 bp
  • G to A, chromosome 2 at 24,404,794 bp
  • T to C, chromosome 2 at 82,984,953 bp
  • C to T, chromosome 2 at 126,127,043 bp
  • A to G, chromosome 3 at 40,513,091 bp
  • G to T, chromosome 4 at 19,055,095 bp
  • T to C, chromosome 4 at 106,748,366 bp
  • T to C, chromosome 4 at 144,395,972 bp
  • A to T, chromosome 5 at 45,676,644 bp
  • G to A, chromosome 5 at 113,258,111 bp
  • C to T, chromosome 7 at 19,184,941 bp
  • T to C, chromosome 8 at 13,598,259 bp
  • A to T, chromosome 9 at 5,352,742 bp
  • A to G, chromosome 10 at 87,290,820 bp
  • T to C, chromosome 11 at 59,131,646 bp
  • T to G, chromosome 11 at 70,253,063 bp
  • T to C, chromosome 11 at 86,299,054 bp
  • A to G, chromosome 12 at 4,943,223 bp
  • A to G, chromosome 12 at 101,591,540 bp
  • T to C, chromosome 12 at 103,606,933 bp
  • T to A, chromosome 13 at 14,247,605 bp
  • A to G, chromosome 13 at 22,726,411 bp
  • T to A, chromosome 13 at 33,154,499 bp
  • A to G, chromosome 13 at 84,251,244 bp
  • T to C, chromosome 14 at 29,103,713 bp
  • C to A, chromosome 16 at 23,077,418 bp
  • G to A, chromosome 16 at 33,987,208 bp
  • T to C, chromosome 17 at 35,428,315 bp
  • A to G, chromosome 18 at 37,309,964 bp
  • G to A, chromosome 19 at 24,030,549 bp
  • G to A, chromosome X at 53,260,677 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4558 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
042005-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.