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Strain Name:
C57BL/6J-MtgxR4620Btlr/Mmmh
Stock Number:
042008-MU
Citation ID:
RRID:MMRRC_042008-MU
Other Names:
R4620 (G1), C57BL/6J-MtgxR4620Btlr
Major Collection:

Strain Information

Chat
Name: choline O-acetyltransferase
Synonyms: B230380D24Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 12647
VEGA: 14
HGNC: HGNC:1912
Homologene: 40693
Ubap2
Name: ubiquitin-associated protein 2
Synonyms: 1190005K07Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 68926
Homologene: 73649
Ranbp1
Name: RAN binding protein 1
Synonyms: Htf9a
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 19385
HGNC: HGNC:9847
Homologene: 21600
Apod
Name: apolipoprotein D
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 11815
HGNC: HGNC:612
Homologene: 1246
Hdgf
Name: heparin binding growth factor
Synonyms: D3Ertd299e
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 15191
HGNC: HGNC:4856
Homologene: 3306
Gart
Name: phosphoribosylglycinamide formyltransferase
Synonyms: Gaps, Prgs
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 14450
HGNC: HGNC:4163
Homologene: 637
Wdfy3
Name: WD repeat and FYVE domain containing 3
Synonyms: 2610509D04Rik, Ggtb3, D5Ertd66e, Bwf1, Bchs, Alfy
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 72145
Homologene: 22855
Eml4
Name: echinoderm microtubule associated protein like 4
Synonyms: 4930443C24Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 78798
VEGA: 17
HGNC: HGNC:1316
Homologene: 56841
Ociad1
Name: OCIA domain containing 1
Synonyms: expressed during mesenchymal induction 2, Emi2, B230209J16Rik, 6030432N09Rik, TPA018, Asrij, Imi2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 68095
Homologene: 9866
Usp32
Name: ubiquitin specific peptidase 32
Synonyms: 6430526O11Rik, 2900074J03Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 237898
Homologene: 13066
Brd7
Name: bromodomain containing 7
Synonyms: bromodomain protein 75 kDa, BP75, CELTIX1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 26992
Homologene: 8085
Birc6
Name: baculoviral IAP repeat-containing 6
Synonyms: apollon, Bruce, A430032G04Rik, D630005A10Rik, A430040A19Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 12211
Homologene: 7248
Cdk5rap2
Name: CDK5 regulatory subunit associated protein 2
Synonyms: 2900018K03Rik, an
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 214444
Homologene: 49533
Xpo4
Name: exportin 4
Synonyms: B430309A01Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 57258
Homologene: 10733
Srbd1
Name: S1 RNA binding domain 1
Synonyms: D530025C17Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 78586
VEGA: 17
Homologene: 9994
Cc2d1b
Name: coiled-coil and C2 domain containing 1B
Synonyms: A830039B04Rik, Freud2
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 319965
Homologene: 70951
Npepps
Name: aminopeptidase puromycin sensitive
Synonyms: MP100, Psa
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 19155
HGNC: HGNC:7900
Homologene: 36199
Senp3
Name: SUMO/sentrin specific peptidase 3
Synonyms: Smt3ip1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 80886
Homologene: 9236
Orc5
Name: origin recognition complex, subunit 5
Synonyms: mouse origin recognition complex 5, MmORC5, Orc5l
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 26429
HGNC: HGNC:8491
Homologene: 37636
Trio
Name: triple functional domain (PTPRF interacting)
Synonyms: 6720464I07Rik, Solo
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 223435
VEGA: 15
Homologene: 20847
Zbtb21
Name: zinc finger and BTB domain containing 21
Synonyms: Znf295, 5430437K12Rik, B430213I24Rik, Zfp295
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 114565
VEGA: 16
Homologene: 10799
Rag1
Name: recombination activating 1
Synonyms: Rag-1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 19373
HGNC: HGNC:9831
Homologene: 387
Prpf39
Name: pre-mRNA processing factor 39
Synonyms: Srcs1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 328110
Homologene: 32377
Sbf1
Name: SET binding factor 1
Synonyms: Mtmr5, B230113C15Rik, 2610510A08Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 77980
Homologene: 84710
Sptb
Name: spectrin beta, erythrocytic
Synonyms: Spnb-1, spectrin R, D330027P03Rik, LOC383567, brain erythroid spectrin (235E), Spnb1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 20741
Homologene: 295
Dnaja3
Name: DnaJ heat shock protein family (Hsp40) member A3
Synonyms: 1200003J13Rik, Tid-1, 1810053A11Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 83945
Homologene: 36170
Kalrn
Name: kalirin, RhoGEF kinase
Synonyms: LOC224126, Hapip, 2210407G14Rik, E530005C20Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 545156
HGNC: HGNC:4814
Homologene: 57160
Sucnr1
Name: succinate receptor 1
Synonyms: Gpr91
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 84112
HGNC: HGNC:4542
Homologene: 41865
B3gnt5
Name: UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 108105
Homologene: 24989
Csmd1
Name: CUB and Sushi multiple domains 1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 94109
Homologene: 69536
Eci3
Name: enoyl-Coenzyme A delta isomerase 3
Synonyms: 1810022C23Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 69123
Homologene: 74357
Dnai3
Name: dynein axonemal intermediate chain 3
Synonyms: 4931433A13Rik, IC140, Ida7, Wdr63
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 242253
Homologene: 44922
Fat3
Name: FAT atypical cadherin 3
Synonyms: LOC234973, LOC382129, 9430076A06Rik, D430038H04Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 270120
VEGA: 9
Homologene: 82252
Miox
Name: myo-inositol oxygenase
Synonyms: RSOR, 0610009I10Rik, C85427, Aldrl6
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 56727
Homologene: 9732
Tmem62
Name: transmembrane protein 62
Synonyms: B830009D23Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 96957
Homologene: 11801
Csmd3
Name: CUB and Sushi multiple domains 3
Synonyms: 4930500N14Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 239420
Homologene: 65982
Kif6
Name: kinesin family member 6
Synonyms: D130004B10Rik, D130084M03Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 319991
Homologene: 35308
Rab11fip1
Name: RAB11 family interacting protein 1 (class I)
Synonyms: 2010200K21Rik, 4833414G05Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 75767
Homologene: 11853
Gas2l2
Name: growth arrest-specific 2 like 2
Synonyms: OTTMUSG00000000934
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 237891
Homologene: 16409
Dll1
Name: delta like canonical Notch ligand 1
Synonyms: Delta1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 13388
HGNC: HGNC:2908
Homologene: 4104
Megf8
Name: multiple EGF-like-domains 8
Synonyms: Egfl4, b2b288Clo, b2b1702Clo, m687Ddg
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 269878
HGNC: HGNC:3233
Homologene: 15988
Pappa
Name: pregnancy-associated plasma protein A
Synonyms: PAG1, IGFBP-4ase, PAPP-A, 8430414N03Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 18491
HGNC: HGNC:8602
Homologene: 31097
Hoxb3
Name: homeobox B3
Synonyms: Hox-2.7
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 15410
HGNC: HGNC:5114
Homologene: 1617
Frem3
Name: Fras1 related extracellular matrix protein 3
Synonyms: LOC333315
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 333315
Homologene: 35388
Dmrta1
Name: doublesex and mab-3 related transcription factor like family A1
Synonyms: Dmrt4
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 242523
Homologene: 18477
Tmprss15
Name: transmembrane protease, serine 15
Synonyms: enteropeptidase, enterokinase, A130097D21Rik, Prss7
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 19146
HGNC: HGNC:9490
Homologene: 2075
Themis2
Name: thymocyte selection associated family member 2
Synonyms: ICB-1, BC013712
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230787
Homologene: 21009
Cdh2
Name: cadherin 2
Synonyms: N-cadherin, Ncad, N-CAD
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 12558
HGNC: HGNC:1759
Homologene: 20424
Postn
Name: periostin, osteoblast specific factor
Synonyms: OSF-2, Periostin, Osf2, peri, A630052E07Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 50706
Homologene: 4730
Cdcp2
Name: CUB domain containing protein 2
Synonyms: D030010E02Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 242603
Homologene: 138406
Pnliprp2
Name: pancreatic lipase-related protein 2
Synonyms: PLRP2
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 18947
VEGA: 19
HGNC: HGNC:9157
Homologene: 3936
Itgav
Name: integrin alpha V
Synonyms: vitronectin receptor alpha polypeptide (VNRA), CD51, alphav-integrin, 1110004F14Rik, 2610028E01Rik, D430040G12Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 16410
HGNC: HGNC:6150
Homologene: 20510
Tmem168
Name: transmembrane protein 168
Synonyms: 5730526F17Rik, 8430437G11Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 101118
Homologene: 11202
Krt2
Name: keratin 2
Synonyms: Krt2-2, Krt2e, Krt2-17
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 16681
HGNC: HGNC:6439
Cenpj
Name: centromere protein J
Synonyms: 4932437H03Rik, Sas4
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 219103
Homologene: 10204
Pde6a
Name: phosphodiesterase 6A, cGMP-specific, rod, alpha
Synonyms: Pdea
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 225600
HGNC: HGNC:8785
Homologene: 380
Sorcs2
Name: sortilin-related VPS10 domain containing receptor 2
Synonyms: VPS10 domain receptor protein
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 81840
Homologene: 56899
Nags
Name: N-acetylglutamate synthase
Synonyms: 1700120E20Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 217214
Homologene: 77363
Rexo5
Name: RNA exonuclease 5
Synonyms: 2610020H08Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 434234
Homologene: 12803
Apba2
Name: amyloid beta precursor protein binding family A member 2
Synonyms: X11-like, X11L, Mint 2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 11784
HGNC: HGNC:579
Homologene: 4021
Or52e15
Name: olfactory receptor family 52 subfamily E member 15
Synonyms: GA_x6K02T2PBJ9-7625746-7624808, MOR32-4, Olfr672
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 258755
Homologene: 138317
Zfp780b
Name: zinc finger protein 780B
Synonyms: B230208L21Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 338354
Homologene: 85969
Kcnh3
Name: potassium voltage-gated channel, subfamily H (eag-related), member 3
Synonyms: ether a go-go like, Elk2, Melk2, C030044P22Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 16512
VEGA: 15
HGNC: HGNC:6252
Homologene: 32150
Cyp2c68
Name: cytochrome P450, family 2, subfamily c, polypeptide 68
Synonyms: 9030012A22Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 433247
Homologene: 74936
Or52e19
Name: olfactory receptor family 52 subfamily E member 19
Synonyms: GA_x6K02T2PBJ9-6019769-6019943, ENSMUSG00000073953, Olfr596-ps1, Gm15117, Olfr596
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 100041187
Homologene: 121535
Rragc
Name: Ras-related GTP binding C
Synonyms: YGR163W, TIB929, Gtr2
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 54170
Homologene: 39141
Blvra
Name: biliverdin reductase A
Synonyms: Blvr, 0610006A11Rik, 2500001N03Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 109778
HGNC: HGNC:1062
Homologene: 572
Or10d5j
Name: olfactory receptor family 10 subfamily D member 5J
Synonyms: GA_x6K02T2PVTD-33657378-33656440, MOR224-10, Olfr976
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 258364
VEGA: 9
Homologene: 64863
Pxdc1
Name: PX domain containing 1
Synonyms: 1300014I06Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 66895
Homologene: 12051
H2-M3
Name: histocompatibility 2, M region locus 3
Synonyms: Hmt, H-2M3, R4B2
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 14991
HGNC: HGNC:4964
Homologene: 133255
Or8b12c
Name: olfactory receptor family 8 subfamily B member 12C
Synonyms: GA_x6K02T2PVTD-31489645-31490577, MOR161-1, Olfr876
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 258883
Homologene: 74154
Ndufb7
Name: NADH:ubiquinone oxidoreductase subunit B7
Synonyms: 1110002H15Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 66916
HGNC: HGNC:7702
Homologene: 3059
Rps23-ps1
Name: ribosomal protein S23, pseudogene 1
Synonyms: Gm15450, Rps23-ps
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 100038875
Trav5-4
Name: T cell receptor alpha variable 5-4
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 21507
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 2 at 83,755,902 bp
  • A to T, chromosome 2 at 101,643,680 bp
  • A to G, chromosome 2 at 120,996,364 bp
  • T to C, chromosome 2 at 127,096,965 bp
  • A to G, chromosome 3 at 37,714,773 bp
  • A to C, chromosome 3 at 54,376,993 bp
  • A to G, chromosome 3 at 60,086,769 bp
  • A to G, chromosome 3 at 87,914,576 bp
  • A to T, chromosome 3 at 146,042,809 bp
  • C to T, chromosome 4 at 41,233,698 bp
  • A to G, chromosome 4 at 65,327,028 bp
  • A to G, chromosome 4 at 70,266,706 bp
  • A to T, chromosome 4 at 89,688,784 bp
  • A to G, chromosome 4 at 107,106,730 bp
  • A to G, chromosome 4 at 108,631,669 bp
  • A to G, chromosome 4 at 123,924,829 bp
  • A to G, chromosome 4 at 132,786,022 bp
  • G to A, chromosome 5 at 22,018,073 bp
  • A to T, chromosome 5 at 22,529,176 bp
  • C to T, chromosome 5 at 36,037,494 bp
  • G to A, chromosome 5 at 73,304,701 bp
  • A to T, chromosome 5 at 101,906,145 bp
  • T to G, chromosome 6 at 13,594,953 bp
  • G to A, chromosome 7 at 25,355,098 bp
  • A to T, chromosome 7 at 27,962,753 bp
  • C to T, chromosome 7 at 64,714,467 bp
  • T to C, chromosome 7 at 98,129,371 bp
  • C to T, chromosome 7 at 103,309,958 bp
  • T to A, chromosome 7 at 104,996,623 bp
  • A to G, chromosome 7 at 119,827,303 bp
  • A to T, chromosome 7 at 131,343,606 bp
  • T to C, chromosome 8 at 16,002,694 bp
  • T to A, chromosome 8 at 27,154,215 bp
  • T to C, chromosome 8 at 80,668,957 bp
  • T to C, chromosome 8 at 83,566,858 bp
  • T to C, chromosome 8 at 88,334,151 bp
  • A to T, chromosome 9 at 15,996,894 bp
  • A to G, chromosome 9 at 37,804,819 bp
  • C to T, chromosome 9 at 39,956,909 bp
  • T to A, chromosome 10 at 92,969,757 bp
  • T to A, chromosome 11 at 69,677,118 bp
  • G to A, chromosome 11 at 77,817,947 bp
  • G to A, chromosome 11 at 82,961,652 bp
  • T to C, chromosome 11 at 83,423,098 bp
  • C to A, chromosome 11 at 85,059,127 bp
  • A to T, chromosome 11 at 96,345,773 bp
  • A to T, chromosome 11 at 97,238,244 bp
  • A to T, chromosome 11 at 102,146,732 bp
  • T to C, chromosome 12 at 65,042,563 bp
  • A to T, chromosome 12 at 76,583,807 bp
  • A to G, chromosome 13 at 34,652,314 bp
  • A to T, chromosome 13 at 34,948,758 bp
  • A to G, chromosome 14 at 32,453,818 bp
  • G to T, chromosome 14 at 53,704,396 bp
  • A to G, chromosome 14 at 56,535,454 bp
  • A to T, chromosome 14 at 57,630,325 bp
  • T to A, chromosome 15 at 27,871,171 bp
  • T to C, chromosome 15 at 47,585,753 bp
  • A to G, chromosome 15 at 89,306,926 bp
  • A to T, chromosome 15 at 89,336,121 bp
  • G to A, chromosome 15 at 99,234,101 bp
  • T to A, chromosome 15 at 101,817,591 bp
  • C to A, chromosome 16 at 4,690,028 bp
  • A to G, chromosome 16 at 18,240,104 bp
  • A to T, chromosome 16 at 19,769,882 bp
  • T to C, chromosome 16 at 31,297,393 bp
  • T to G, chromosome 16 at 34,028,705 bp
  • T to C, chromosome 16 at 79,021,470 bp
  • T to C, chromosome 16 at 91,625,433 bp
  • T to C, chromosome 16 at 97,949,892 bp
  • C to T, chromosome 17 at 15,370,566 bp
  • C to A, chromosome 17 at 37,272,419 bp
  • T to C, chromosome 17 at 49,901,296 bp
  • A to C, chromosome 17 at 74,640,150 bp
  • T to C, chromosome 17 at 83,461,533 bp
  • A to T, chromosome 17 at 86,109,265 bp
  • A to G, chromosome 18 at 16,648,608 bp
  • A to G, chromosome 18 at 61,262,492 bp
  • A to T, chromosome 19 at 39,712,562 bp
  • G to A, chromosome 19 at 58,762,286 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4620 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042008-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.