Strain Name:
C57BL/6J-MtgxR4748Btlr/Mmmh
Stock Number:
042030-MU
Citation ID:
RRID:MMRRC_042030-MU
Other Names:
R4748 (G1), C57BL/6J-MtgxR4748Btlr
Major Collection:

Gene Information

Gene Symbol: Shprh [MGI:1917581] (Mus musculus (mouse))
Name: SNF2 histone linker PHD RING helicase
Synonyms: 2610103K11Rik, D230017O13Rik
Chromosome: 10
Alteration at locus: Chemically Induced
Gene Symbol: Otx1 [MGI:97450] (Mus musculus (mouse))
Name: orthodenticle homeobox 1
Synonyms: A730044F23Rik, jv
Chromosome: 11
Alteration at locus: Chemically Induced
Gene Symbol: D630045J12Rik [MGI:2669829] (Mus musculus (mouse))
Name: RIKEN cDNA D630045J12 gene
Chromosome: 6
Alteration at locus: Chemically Induced
Gene Symbol: Frem2 [MGI:2444465] (Mus musculus (mouse))
Name: Fras1 related extracellular matrix protein 2
Synonyms: 8430406N05Rik, my, 6030440P17Rik, b2b1562Clo, ne
Chromosome: 3
Alteration at locus: Chemically Induced
Gene Symbol: Sgsm1 [MGI:107320] (Mus musculus (mouse))
Name: small G protein signaling modulator 1
Synonyms: D5Bwg1524e, Rutbc2, 2410098H20Rik
Chromosome: 5
Alteration at locus: Chemically Induced
Gene Symbol: Ptpn12 [MGI:104673] (Mus musculus (mouse))
Name: protein tyrosine phosphatase, non-receptor type 12
Synonyms: PTP-PEST, PTP-P19, P19-PTP
Chromosome: 5
Alteration at locus: Chemically Induced
Gene Symbol: Rabep1 [MGI:1860236] (Mus musculus (mouse))
Name: rabaptin, RAB GTPase binding effector protein 1
Synonyms: rabaptin-5, RAB5 effector protein, neurocrescin
Chromosome: 11
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 20,935,413 bp
  • T to C, chromosome 1 at 23,101,870 bp
  • G to T, chromosome 1 at 44,013,578 bp
  • C to T, chromosome 1 at 74,421,266 bp
  • C to A, chromosome 1 at 75,177,358 bp
  • T to A, chromosome 1 at 134,041,174 bp
  • C to A, chromosome 1 at 134,542,028 bp
  • T to A, chromosome 1 at 138,856,656 bp
  • G to A, chromosome 1 at 171,219,562 bp
  • A to C, chromosome 2 at 77,057,980 bp
  • T to A, chromosome 2 at 88,070,612 bp
  • C to T, chromosome 2 at 89,406,255 bp
  • A to T, chromosome 2 at 105,378,646 bp
  • T to C, chromosome 2 at 112,964,405 bp
  • A to T, chromosome 2 at 127,336,907 bp
  • G to A, chromosome 2 at 129,002,240 bp
  • G to A, chromosome 2 at 158,272,021 bp
  • A to G, chromosome 3 at 53,541,093 bp
  • G to A, chromosome 3 at 57,646,794 bp
  • C to T, chromosome 3 at 87,089,151 bp
  • A to T, chromosome 3 at 106,122,449 bp
  • A to G, chromosome 3 at 129,332,163 bp
  • A to G, chromosome 3 at 142,538,087 bp
  • A to C, chromosome 4 at 43,030,055 bp
  • A to G, chromosome 4 at 52,856,076 bp
  • T to C, chromosome 4 at 62,524,875 bp
  • A to T, chromosome 4 at 87,840,781 bp
  • T to A, chromosome 4 at 99,981,979 bp
  • G to A, chromosome 4 at 118,389,191 bp
  • G to T, chromosome 5 at 9,130,698 bp
  • A to T, chromosome 5 at 21,005,385 bp
  • C to A, chromosome 5 at 88,501,543 bp
  • A to C, chromosome 5 at 93,392,130 bp
  • T to C, chromosome 5 at 110,855,839 bp
  • A to T, chromosome 5 at 113,285,592 bp
  • A to G, chromosome 5 at 130,236,890 bp
  • G to A, chromosome 6 at 24,137,027 bp
  • T to A, chromosome 6 at 36,937,327 bp
  • T to C, chromosome 6 at 38,196,841 bp
  • A to G, chromosome 6 at 48,064,556 bp
  • A to G, chromosome 6 at 86,628,423 bp
  • A to C, chromosome 7 at 24,781,052 bp
  • A to T, chromosome 7 at 42,267,141 bp
  • C to T, chromosome 7 at 42,928,120 bp
  • T to C, chromosome 7 at 63,735,915 bp
  • G to T, chromosome 7 at 80,290,048 bp
  • C to T, chromosome 7 at 132,705,735 bp
  • T to C, chromosome 7 at 141,071,720 bp
  • G to A, chromosome 8 at 4,744,512 bp
  • A to G, chromosome 8 at 4,813,143 bp
  • A to G, chromosome 8 at 13,123,526 bp
  • C to A, chromosome 8 at 14,745,433 bp
  • A to G, chromosome 8 at 71,580,675 bp
  • C to T, chromosome 8 at 71,580,809 bp
  • T to C, chromosome 8 at 80,611,459 bp
  • T to A, chromosome 8 at 110,088,672 bp
  • A to T, chromosome 9 at 53,771,785 bp
  • G to C, chromosome 9 at 70,581,365 bp
  • C to A, chromosome 9 at 82,908,869 bp
  • A to G, chromosome 9 at 99,642,651 bp
  • G to A, chromosome 10 at 11,170,476 bp
  • T to C, chromosome 10 at 49,535,341 bp
  • C to T, chromosome 10 at 52,115,997 bp
  • A to T, chromosome 10 at 59,424,082 bp
  • A to G, chromosome 10 at 60,716,326 bp
  • C to T, chromosome 10 at 81,086,047 bp
  • T to A, chromosome 10 at 88,675,890 bp
  • T to C, chromosome 10 at 120,084,849 bp
  • T to A, chromosome 11 at 6,271,716 bp
  • A to G, chromosome 11 at 21,998,690 bp
  • T to A, chromosome 11 at 70,908,468 bp
  • C to T, chromosome 11 at 78,154,603 bp
  • T to C, chromosome 11 at 98,041,372 bp
  • C to T, chromosome 11 at 101,072,021 bp
  • T to C, chromosome 11 at 107,095,880 bp
  • C to A, chromosome 11 at 115,448,138 bp
  • T to C, chromosome 11 at 116,843,594 bp
  • T to C, chromosome 11 at 120,701,507 bp
  • C to A, chromosome 12 at 69,174,462 bp
  • T to C, chromosome 12 at 70,248,273 bp
  • T to A, chromosome 12 at 71,151,397 bp
  • T to C, chromosome 12 at 80,480,576 bp
  • A to G, chromosome 12 at 81,378,056 bp
  • T to G, chromosome 12 at 84,147,723 bp
  • T to A, chromosome 13 at 46,639,826 bp
  • T to A, chromosome 13 at 85,189,639 bp
  • A to G, chromosome 14 at 31,319,945 bp
  • T to G, chromosome 14 at 50,413,876 bp
  • C to T, chromosome 14 at 79,411,650 bp
  • C to A, chromosome 15 at 66,906,640 bp
  • T to A, chromosome 15 at 71,464,055 bp
  • G to A, chromosome 15 at 96,420,421 bp
  • G to A, chromosome 15 at 101,555,333 bp
  • T to A, chromosome 15 at 103,473,056 bp
  • A to T, chromosome 18 at 39,774,269 bp
  • G to A, chromosome 18 at 44,082,361 bp
  • A to C, chromosome 18 at 60,550,236 bp
  • C to T, chromosome 19 at 7,925,876 bp
  • G to T, chromosome 19 at 10,794,225 bp
  • T to A, chromosome 19 at 41,944,558 bp
  • T to C, chromosome 19 at 44,016,737 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4748 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
042030-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.