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Strain Name:
C57BL/6J-MtgxR4783Btlr/Mmmh
Stock Number:
042416-MU
Citation ID:
RRID:MMRRC_042416-MU
Other Names:
R4783 (G1), C57BL/6J-MtgxR4783Btlr
Major Collection:

Strain Information

Hlcs
Name: holocarboxylase synthetase (biotin- [propriony-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase)
Synonyms: 410I21.SP6, D16Jhu34
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 110948
VEGA: 16
HGNC: HGNC:4976
Homologene: 37302
Elf1
Name: E74 like ETS transcription factor 1
Synonyms: Elf-1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 13709
VEGA: 14
HGNC: HGNC:3316
Homologene: 7303
Rprd2
Name: regulation of nuclear pre-mRNA domain containing 2
Synonyms: 6720469I21Rik, 2810036A19Rik, 4930535B03Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 75137
Homologene: 19997
Cdr2
Name: cerebellar degeneration-related 2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 12585
HGNC: HGNC:1799
Homologene: 7262
Hmgcr
Name: 3-hydroxy-3-methylglutaryl-Coenzyme A reductase
Synonyms: HMG-CoAR, 3-hydroxy-3-methylglutaryl-CoA reductase, Red
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 15357
HGNC: HGNC:5006
Homologene: 30994
Pik3c2a
Name: phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 alpha
Synonyms: PI3KC2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 18704
HGNC: HGNC:8971
Homologene: 20581
Rbm6
Name: RNA binding motif protein 6
Synonyms: NY-LU-12, g16, Def-3
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 19654
HGNC: HGNC:9903
Homologene: 31336
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 36,779,173 bp
  • A to G, chromosome 1 at 86,078,712 bp
  • A to G, chromosome 1 at 107,604,742 bp
  • A to G, chromosome 1 at 146,727,640 bp
  • A to T, chromosome 1 at 173,480,866 bp
  • T to C, chromosome 1 at 178,111,590 bp
  • T to C, chromosome 2 at 5,054,627 bp
  • T to A, chromosome 2 at 25,292,381 bp
  • T to A, chromosome 2 at 32,297,156 bp
  • T to C, chromosome 2 at 52,112,747 bp
  • T to C, chromosome 2 at 66,540,623 bp
  • G to T, chromosome 2 at 76,721,797 bp
  • A to G, chromosome 2 at 76,769,603 bp
  • A to G, chromosome 2 at 85,590,938 bp
  • A to G, chromosome 2 at 88,323,156 bp
  • A to T, chromosome 2 at 111,873,050 bp
  • G to A, chromosome 2 at 112,248,936 bp
  • T to C, chromosome 2 at 118,114,792 bp
  • A to T, chromosome 2 at 121,008,522 bp
  • T to C, chromosome 2 at 121,340,848 bp
  • A to T, chromosome 2 at 125,324,919 bp
  • T to C, chromosome 2 at 143,687,679 bp
  • T to C, chromosome 2 at 156,096,564 bp
  • C to A, chromosome 3 at 35,975,873 bp
  • G to T, chromosome 3 at 85,688,570 bp
  • A to G, chromosome 3 at 95,774,333 bp
  • A to G, chromosome 3 at 158,127,213 bp
  • T to C, chromosome 4 at 41,758,189 bp
  • T to A, chromosome 4 at 44,570,086 bp
  • A to T, chromosome 4 at 126,368,503 bp
  • A to T, chromosome 4 at 132,902,117 bp
  • A to G, chromosome 4 at 132,916,190 bp
  • A to T, chromosome 4 at 134,344,006 bp
  • G to T, chromosome 4 at 139,300,001 bp
  • T to C, chromosome 4 at 139,421,733 bp
  • G to A, chromosome 4 at 152,554,546 bp
  • G to A, chromosome 5 at 30,105,200 bp
  • T to C, chromosome 5 at 86,891,473 bp
  • A to T, chromosome 5 at 109,086,513 bp
  • C to A, chromosome 5 at 120,814,513 bp
  • C to A, chromosome 5 at 124,636,079 bp
  • T to A, chromosome 7 at 6,305,685 bp
  • A to G, chromosome 7 at 6,961,391 bp
  • A to T, chromosome 7 at 13,084,861 bp
  • A to G, chromosome 7 at 29,377,641 bp
  • A to G, chromosome 7 at 47,335,470 bp
  • T to C, chromosome 7 at 98,646,479 bp
  • A to G, chromosome 7 at 102,013,566 bp
  • A to T, chromosome 7 at 116,417,825 bp
  • A to T, chromosome 7 at 120,958,421 bp
  • T to C, chromosome 8 at 9,208,026 bp
  • G to A, chromosome 9 at 3,006,915 bp
  • T to C, chromosome 9 at 107,852,903 bp
  • A to G, chromosome 10 at 78,464,792 bp
  • A to T, chromosome 10 at 94,910,629 bp
  • G to A, chromosome 11 at 4,646,653 bp
  • A to G, chromosome 11 at 50,171,298 bp
  • A to T, chromosome 11 at 54,336,993 bp
  • T to A, chromosome 11 at 58,988,924 bp
  • A to T, chromosome 11 at 67,341,270 bp
  • A to G, chromosome 11 at 73,984,008 bp
  • C to T, chromosome 11 at 75,510,641 bp
  • T to G, chromosome 11 at 77,499,837 bp
  • T to A, chromosome 11 at 106,203,604 bp
  • G to A, chromosome 11 at 115,800,362 bp
  • A to G, chromosome 11 at 117,791,605 bp
  • G to A, chromosome 11 at 118,393,514 bp
  • A to G, chromosome 11 at 121,707,924 bp
  • T to A, chromosome 12 at 35,098,286 bp
  • T to A, chromosome 12 at 103,590,858 bp
  • T to C, chromosome 12 at 103,767,824 bp
  • T to C, chromosome 12 at 104,409,110 bp
  • T to A, chromosome 12 at 108,855,334 bp
  • C to T, chromosome 13 at 13,499,741 bp
  • G to A, chromosome 13 at 60,800,395 bp
  • T to C, chromosome 13 at 81,095,445 bp
  • G to A, chromosome 13 at 96,666,193 bp
  • A to T, chromosome 14 at 7,905,701 bp
  • A to C, chromosome 14 at 14,407,729 bp
  • T to A, chromosome 14 at 31,263,479 bp
  • A to G, chromosome 14 at 43,179,966 bp
  • C to A, chromosome 14 at 52,205,368 bp
  • T to C, chromosome 14 at 55,501,321 bp
  • C to T, chromosome 14 at 70,777,874 bp
  • T to A, chromosome 14 at 79,580,743 bp
  • T to A, chromosome 14 at 87,472,101 bp
  • T to C, chromosome 14 at 120,022,364 bp
  • T to C, chromosome 15 at 3,969,041 bp
  • T to C, chromosome 15 at 89,149,488 bp
  • G to A, chromosome 16 at 18,258,310 bp
  • G to T, chromosome 16 at 59,027,897 bp
  • G to T, chromosome 16 at 59,215,859 bp
  • C to T, chromosome 16 at 94,268,539 bp
  • A to T, chromosome 17 at 18,929,288 bp
  • A to G, chromosome 17 at 20,735,134 bp
  • T to A, chromosome 17 at 23,953,330 bp
  • T to A, chromosome 17 at 32,396,781 bp
  • A to G, chromosome 18 at 50,568,065 bp
  • C to A, chromosome 18 at 65,172,927 bp
  • G to A, chromosome 19 at 8,766,798 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4783 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042416-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.