Strain Name:
C57BL/6J-MtgxR4785Btlr/Mmmh
Stock Number:
042417-MU
Citation ID:
RRID:MMRRC_042417-MU
Other Names:
R4785 (G1), C57BL/6J-MtgxR4785Btlr
Major Collection:

Gene Information

Gene Symbol: Utrn [MGI:104631] (Mus musculus (mouse))
Name: utrophin
Synonyms: DRP, G-utrophin, Dmdl
Chromosome: 10
Alteration at locus: Chemically Induced
Gene Symbol: Ccr2 [MGI:106185] (Mus musculus (mouse))
Name: chemokine (C-C motif) receptor 2
Synonyms: CC-CKR-2, Cmkbr2, CKR2, CKR2B, CKR2A, CCR2A, CCR2B
Chromosome: 9
Alteration at locus: Chemically Induced
Gene Symbol: Chrna6 [MGI:106213] (Mus musculus (mouse))
Name: cholinergic receptor, nicotinic, alpha polypeptide 6
Synonyms: Acra6, alpha6 nAChR
Chromosome: 8
Alteration at locus: Chemically Induced
Gene Symbol: Nos1 [MGI:97360] (Mus musculus (mouse))
Name: nitric oxide synthase 1, neuronal
Synonyms: nNOS, bNOS, Nos-1, 2310005C01Rik, NO
Chromosome: 5
Alteration at locus: Chemically Induced
Gene Symbol: Hcfc1 [MGI:105942] (Mus musculus (mouse))
Name: host cell factor C1
Synonyms: HCF1, VP16 accessory protein, HCF-1
Chromosome: X
Alteration at locus: Chemically Induced
Gene Symbol: Vcan [MGI:102889] (Mus musculus (mouse))
Name: versican
Synonyms: DPEAAE, heart defect, Cspg2, PG-M, hdf, 5430420N07Rik
Chromosome: 13
Alteration at locus: Chemically Induced
Gene Symbol: Kat2b [MGI:1343094] (Mus musculus (mouse))
Name: K(lysine) acetyltransferase 2B
Synonyms: A930006P13Rik, Pcaf
Chromosome: 17
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 54,259,995 bp
  • C to T, chromosome 1 at 58,752,567 bp
  • A to G, chromosome 1 at 59,215,313 bp
  • A to G, chromosome 1 at 65,267,846 bp
  • A to T, chromosome 1 at 74,679,007 bp
  • T to C, chromosome 1 at 75,530,281 bp
  • C to T, chromosome 1 at 116,101,565 bp
  • T to C, chromosome 1 at 153,231,740 bp
  • A to T, chromosome 1 at 172,254,110 bp
  • G to T, chromosome 1 at 172,584,214 bp
  • A to T, chromosome 1 at 173,480,866 bp
  • A to G, chromosome 1 at 182,448,997 bp
  • A to T, chromosome 1 at 190,750,188 bp
  • T to A, chromosome 2 at 25,292,381 bp
  • T to A, chromosome 2 at 26,983,042 bp
  • T to A, chromosome 2 at 32,297,156 bp
  • T to C, chromosome 2 at 52,112,747 bp
  • T to A, chromosome 2 at 69,959,603 bp
  • G to T, chromosome 2 at 76,721,797 bp
  • A to G, chromosome 2 at 76,769,603 bp
  • T to C, chromosome 2 at 85,063,034 bp
  • A to G, chromosome 2 at 85,590,938 bp
  • A to T, chromosome 2 at 111,873,050 bp
  • G to A, chromosome 2 at 112,248,936 bp
  • A to C, chromosome 2 at 112,479,585 bp
  • A to T, chromosome 2 at 121,008,522 bp
  • A to T, chromosome 2 at 125,324,919 bp
  • A to G, chromosome 2 at 125,586,329 bp
  • T to C, chromosome 2 at 156,096,564 bp
  • C to A, chromosome 3 at 35,975,873 bp
  • G to T, chromosome 3 at 85,688,570 bp
  • T to C, chromosome 3 at 87,922,050 bp
  • A to G, chromosome 3 at 95,134,986 bp
  • C to A, chromosome 3 at 102,853,489 bp
  • A to C, chromosome 3 at 107,624,393 bp
  • A to T, chromosome 3 at 108,458,227 bp
  • T to C, chromosome 3 at 119,726,225 bp
  • T to C, chromosome 3 at 121,527,074 bp
  • G to T, chromosome 3 at 127,687,592 bp
  • A to T, chromosome 4 at 43,356,980 bp
  • A to C, chromosome 4 at 43,568,315 bp
  • T to C, chromosome 4 at 76,140,553 bp
  • A to T, chromosome 4 at 126,368,503 bp
  • A to T, chromosome 4 at 132,902,117 bp
  • A to G, chromosome 4 at 132,916,190 bp
  • T to C, chromosome 4 at 138,998,039 bp
  • G to T, chromosome 4 at 139,300,001 bp
  • G to T, chromosome 5 at 24,714,023 bp
  • C to T, chromosome 5 at 86,306,281 bp
  • C to A, chromosome 5 at 88,584,266 bp
  • A to G, chromosome 5 at 100,459,738 bp
  • A to G, chromosome 5 at 114,360,599 bp
  • A to G, chromosome 5 at 117,919,352 bp
  • T to C, chromosome 5 at 123,579,293 bp
  • T to G, chromosome 5 at 123,816,762 bp
  • C to G, chromosome 5 at 123,864,450 bp
  • A to G, chromosome 5 at 129,513,083 bp
  • T to G, chromosome 5 at 135,217,413 bp
  • C to A, chromosome 5 at 138,444,789 bp
  • T to A, chromosome 6 at 8,222,464 bp
  • T to G, chromosome 6 at 42,141,284 bp
  • T to C, chromosome 6 at 43,094,436 bp
  • A to G, chromosome 6 at 85,961,509 bp
  • G to T, chromosome 6 at 88,618,021 bp
  • T to C, chromosome 6 at 124,437,372 bp
  • T to C, chromosome 6 at 127,148,798 bp
  • A to T, chromosome 7 at 5,611,125 bp
  • A to T, chromosome 7 at 5,611,127 bp
  • A to G, chromosome 7 at 6,961,391 bp
  • A to T, chromosome 7 at 13,084,861 bp
  • G to A, chromosome 7 at 26,312,875 bp
  • A to G, chromosome 7 at 29,377,641 bp
  • A to G, chromosome 7 at 45,856,781 bp
  • A to G, chromosome 7 at 47,335,470 bp
  • C to A, chromosome 7 at 86,363,735 bp
  • T to C, chromosome 7 at 86,694,528 bp
  • A to C, chromosome 7 at 96,774,046 bp
  • G to A, chromosome 7 at 102,113,219 bp
  • C to T, chromosome 7 at 111,076,796 bp
  • T to A, chromosome 7 at 119,967,824 bp
  • T to A, chromosome 7 at 135,268,788 bp
  • T to A, chromosome 8 at 27,407,106 bp
  • T to A, chromosome 8 at 34,111,679 bp
  • T to A, chromosome 8 at 40,911,058 bp
  • A to T, chromosome 9 at 24,424,823 bp
  • A to G, chromosome 9 at 32,129,653 bp
  • T to C, chromosome 9 at 32,260,780 bp
  • T to C, chromosome 9 at 44,513,341 bp
  • A to G, chromosome 9 at 65,413,448 bp
  • C to A, chromosome 9 at 79,678,494 bp
  • T to A, chromosome 9 at 80,286,447 bp
  • A to G, chromosome 9 at 80,398,450 bp
  • A to T, chromosome 9 at 87,019,796 bp
  • T to C, chromosome 9 at 102,629,821 bp
  • T to C, chromosome 9 at 107,787,352 bp
  • A to G, chromosome 9 at 109,724,800 bp
  • G to A, chromosome 9 at 124,106,372 bp
  • T to G, chromosome 10 at 12,654,745 bp
  • T to A, chromosome 10 at 64,088,002 bp
  • A to G, chromosome 10 at 76,226,221 bp
  • TTCTCTCTCTCTCTCTCT to TTCTCTCTCTCTCTCT, chromosome 10 at 80,712,778 bp
  • C to T, chromosome 10 at 81,183,144 bp
  • A to T, chromosome 10 at 82,660,749 bp
  • A to T, chromosome 10 at 90,914,750 bp
  • T to C, chromosome 10 at 127,558,133 bp
  • T to C, chromosome 11 at 6,349,875 bp
  • G to A, chromosome 11 at 9,038,266 bp
  • T to A, chromosome 11 at 23,002,866 bp
  • A to G, chromosome 11 at 50,792,722 bp
  • A to G, chromosome 11 at 50,857,277 bp
  • G to A, chromosome 11 at 91,576,428 bp
  • G to A, chromosome 11 at 118,393,514 bp
  • A to G, chromosome 11 at 121,707,924 bp
  • A to G, chromosome 12 at 4,206,542 bp
  • A to T, chromosome 12 at 17,924,519 bp
  • T to C, chromosome 12 at 32,205,199 bp
  • T to C, chromosome 12 at 32,848,714 bp
  • A to G, chromosome 12 at 51,297,568 bp
  • C to A, chromosome 13 at 19,657,930 bp
  • C to T, chromosome 13 at 21,312,264 bp
  • C to T, chromosome 13 at 47,063,077 bp
  • T to A, chromosome 13 at 49,724,663 bp
  • T to C, chromosome 13 at 59,691,592 bp
  • T to C, chromosome 13 at 89,705,789 bp
  • C to G, chromosome 13 at 108,052,780 bp
  • T to C, chromosome 13 at 110,061,900 bp
  • A to T, chromosome 14 at 7,905,701 bp
  • A to C, chromosome 14 at 14,407,729 bp
  • A to T, chromosome 14 at 14,855,714 bp
  • T to A, chromosome 14 at 31,263,479 bp
  • T to G, chromosome 14 at 51,985,337 bp
  • A to T, chromosome 14 at 65,953,501 bp
  • T to A, chromosome 14 at 67,734,164 bp
  • T to C, chromosome 15 at 3,969,041 bp
  • A to T, chromosome 15 at 54,752,207 bp
  • A to G, chromosome 15 at 60,823,932 bp
  • T to C, chromosome 15 at 83,131,501 bp
  • T to G, chromosome 15 at 101,293,528 bp
  • T to C, chromosome 16 at 15,648,976 bp
  • G to A, chromosome 16 at 17,895,062 bp
  • A to C, chromosome 16 at 97,950,455 bp
  • A to T, chromosome 17 at 21,929,419 bp
  • A to G, chromosome 17 at 33,598,191 bp
  • A to T, chromosome 17 at 35,321,126 bp
  • T to C, chromosome 17 at 43,625,576 bp
  • T to A, chromosome 17 at 47,788,227 bp
  • A to G, chromosome 17 at 53,653,203 bp
  • A to T, chromosome 17 at 54,278,700 bp
  • T to A, chromosome 17 at 65,982,999 bp
  • T to A, chromosome 17 at 73,240,070 bp
  • A to G, chromosome 18 at 50,568,065 bp
  • A to G, chromosome 19 at 7,374,091 bp
  • C to A, chromosome 19 at 9,877,690 bp
  • T to A, chromosome 19 at 9,877,691 bp
  • A to T, chromosome 19 at 12,422,977 bp
  • A to G, chromosome 19 at 43,502,937 bp
  • G to T, chromosome 19 at 46,268,395 bp
  • A to G, chromosome 19 at 57,629,158 bp
  • T to C, chromosome X at 73,965,946 bp
  • A to T, chromosome X at 106,437,702 bp
  • A to C, chromosome X at 152,660,907 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4785 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
042417-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.